{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:17:50Z","timestamp":1792624670544,"version":"4.5.0"},"reference-count":83,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,22]],"date-time":"2022-04-22T00:00:00Z","timestamp":1650585600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["UID\/CVT\/00276\/2020"],"award-info":[{"award-number":["UID\/CVT\/00276\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/113142\/2015"],"award-info":[{"award-number":["SFRH\/BD\/113142\/2015"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["2020.07562.BD"],"award-info":[{"award-number":["2020.07562.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","doi-asserted-by":"publisher","award":["JPIAMR\/0002\/2016"],"award-info":[{"award-number":["JPIAMR\/0002\/2016"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Urinary tract infections (UTI) caused by Escherichia coli are frequently diagnosed in humans and companion animals. Extended-spectrum beta-lactamase (ESBL)- and cephalosporinase (pAmpC)-producing Escherichia coli are worldwide-disseminated and frequently multidrug-resistant, hence leading to treatment failure and public health concerns. This study aimed to characterize and compare ESBL\/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and non-related humans. Third-generation cephalosporin (3GC)-resistant E. coli (companion animals n = 35; humans n = 85) isolated from patients with UTI were tested against 14 antimicrobials following CLSI guidelines. PCR-based assays were used to detect the major E. coli phylogenetic groups, pathogenicity associated-islands (PAIs), virulence genes, and ESBLs\/pAmpC resistance genes. ESBL\/pAmpC-producing E. coli isolates were typed by multi-locus sequence typing (MLST) and PCR. E. coli strains from companion animals and humans shared two MDR high-risk clonal lineages: ST131 and ST648. To the best of our knowledge, this study reports the first description of E. coli ST131 clade C1-M27 and the clonal lineage ST131 clade A in humans with community-acquired UTI in Portugal. Considering that companion animals with UTI are generally treated at home by the owners, measures should be implemented to avoid the spread of multidrug-resistant high-risk clones to humans and their household environment.<\/jats:p>","DOI":"10.3390\/antibiotics11050559","type":"journal-article","created":{"date-parts":[[2022,4,23]],"date-time":"2022-04-23T08:14:06Z","timestamp":1650701646000},"page":"559","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["ESBL\/pAmpC-Producing Escherichia coli Causing Urinary Tract Infections in Non-Related Companion Animals and Humans"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6564-6839","authenticated-orcid":false,"given":"Adriana","family":"Belas","sequence":"first","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"},{"name":"Faculty of Veterinary Medicine, Lus\u00f3fona University, 1749-024 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9648-6380","authenticated-orcid":false,"given":"C\u00e1tia","family":"Marques","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"},{"name":"Faculty of Veterinary Medicine, Lus\u00f3fona University, 1749-024 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4392-5372","authenticated-orcid":false,"given":"Juliana","family":"Menezes","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3894-3488","authenticated-orcid":false,"given":"Lu\u00eds Telo","family":"da Gama","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2463-0176","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Cavaco-Silva","sequence":"additional","affiliation":[{"name":"Centro de Investiga\u00e7\u00e3o Interdisciplinar Egas Moniz, Instituto Universit\u00e1rio Egas Moniz, 2829-511 Caparica, Portugal"},{"name":"Technophage, 1649-028 Lisboa, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Constan\u00e7a","family":"Pomba","sequence":"additional","affiliation":[{"name":"Centre for Interdisciplinary Research in Animal Health (CIISA), Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal"},{"name":"Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), 1300-477 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1038\/nrurol.2010.190","article-title":"The epidemiology of urinary tract infection","volume":"7","author":"Foxman","year":"2010","journal-title":"Nat. Rev. Urol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1016\/j.ijfoodmicro.2010.06.025","article-title":"Broiler chikens, broiler chiken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community\u2014Dwelling humans and UTI patients","volume":"142","author":"Jakobsen","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1093\/jac\/dkx401","article-title":"Increase in antimicrobial resistance and emergence of major international high-risk clonal lineages in dogs and cats with urinary tract infection: 16-year retrospective study","volume":"73","author":"Marques","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_5","unstructured":"World Health Organization (WHO) (2022, January 15). Critically Important Antimicrobials for Human Medicine. 6th Revision WHO Advisory Group on Integrated Surveillance of Antimicrobial Resistance (AGISAR). Available online: https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/312266\/9789241515528-eng.pdf."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e1182","DOI":"10.54034\/mic.e1182","article-title":"Antimicrobial Resistance, from Bench-to-Publicside","volume":"1","author":"Ruiz","year":"2021","journal-title":"Microbes Infect. Chemother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1128\/AEM.01324-08","article-title":"Intestine and environment of the chicken as reservoirs for extraintestinal pathogenic Escherichia coli strains with zoonotic potential","volume":"75","author":"Ewers","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1093\/jac\/dkq004","article-title":"Emergence of human pandemic O25:H4-ST131 CTX-M-15 extended-spectrum-beta-lactamase-producing Escherichia coli among companion animals","volume":"65","author":"Ewers","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1007\/s10096-011-1431-7","article-title":"Persistence of uropathogenic Escherichia coli strains in the host for long periods of time: Relationship between phylogenetic groups and virulence factors","volume":"31","author":"Narciso","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1111\/j.1469-0691.2012.03850.x","article-title":"Extended-spectrum \u03b2-lactamase-producing and AmpC-producing Escherichia coli from livestock and companion animals, and their putative impact on public health: A global perspective","volume":"18","author":"Ewers","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"565","DOI":"10.1128\/CMR.00116-14","article-title":"The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae","volume":"28","author":"Mathers","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1007\/s40265-019-01180-3","article-title":"Extended-Spectrum \u03b2-Lactamase-Producing Enterobacteriaceae: Update on molecular epidemiology and treatment options","volume":"79","author":"Peirano","year":"2019","journal-title":"Drugs"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.1093\/jac\/dkt516","article-title":"CTX-M-15-D-ST648 Escherichia coli from companion animals and horses: Another pandemic clone combining multiresistance and extraintestinal virulence?","volume":"69","author":"Ewers","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_14","first-page":"957","article-title":"Public health risk of antimicrobial resistance transfer from companion animals","volume":"72","author":"Pomba","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"3721","DOI":"10.1128\/JCM.01581-09","article-title":"Sharing of Escherichia coli Sequence Type ST131 and other multidrug-resistant and urovirulent E. coli strains among dogs and cats within a household","volume":"47","author":"Johnson","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2733","DOI":"10.1128\/AAC.00297-09","article-title":"Epidemic clonal groups of Escherichia coli as a cause of antimicrobial\u2014Resistant urinary tract infections in Canada, 2002\u20132004","volume":"53","author":"Johnson","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1093\/jac\/dkt343","article-title":"Within-lineage variability of ST131 Escherichia coli isolates from humans and companion animals in the south of Europe","volume":"69","author":"Pomba","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1186\/s13756-018-0427-9","article-title":"Intestinal colonization due to Escherichia coli ST131: Risk factors and prevalence","volume":"7","author":"Barroso","year":"2018","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_19","first-page":"266","article-title":"Emergence of Escherichia coli ST131 H30\/H30-Rx subclones in companion animals","volume":"74","author":"Belas","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_20","first-page":"1057","article-title":"Occurrence of OXA-48 carbapenemases and other \u03b2-lactamase genes in ESBL-producing multidrug resistant Escherichia coli from dogs and cats in the United States, 2009\u20132013","volume":"7","author":"Liu","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1111\/j.1469-0691.2006.01461.x","article-title":"Pathogenicity Island markers in commensal and uropathogenic Escherichia coli isolates","volume":"12","author":"Moreno","year":"2006","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/s13099-019-0290-0","article-title":"Virulence factors, prevalence and potential transmission of extraintestinal pathogenic Escherichia coli isolated from different sources: Recent reports","volume":"11","author":"Sarowska","year":"2019","journal-title":"Gut Pathog."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1093\/infdis\/jit480","article-title":"Escherichia coli uropathogenic-specific protein, Usp, is a bacteriocin-like genotoxin","volume":"208","author":"Podlesek","year":"2013","journal-title":"J. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Crnigoj, M., Podlesek, Z., Budi\u010d, M., and Zgur-Bertok, D. (2014). The Escherichia coli uropathogenic-specific-protein-associated immunity protein 3 (Imu3) has nucleic acid -binding activity. BMC Microbiol., 14.","DOI":"10.1186\/1471-2180-14-16"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1900","DOI":"10.3201\/eid2211.160519","article-title":"Global Escherichia coli sequence Type 131 clade with blaCTX-M-27 gene","volume":"22","author":"Matsumura","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1093\/jac\/dkv485","article-title":"Diversity of STs, plasmids and ESBL genes among Escherichia coli from humans, animals and food in Germany, the Netherlands and the UK","volume":"71","author":"Day","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4705","DOI":"10.1128\/AEM.00495-16","article-title":"Host-specific patterns of genetic diversity among IncI1-Igamma and IncK plasmids encoding CMY-2 beta-lactamase in Escherichia coli isolates from humans, poultry meat, poultry, and dogs in Denmark","volume":"82","author":"Hansen","year":"2016","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1111\/j.1348-0421.2012.00463.x","article-title":"Mechanisms of resistance to cephalosporin and emergence of O25b-ST131 clone harboring CTX-M-27 \u03b2-lactamase in extraintestinal pathogenic Escherichia coli from dogs and cats in Japan","volume":"56","author":"Harada","year":"2012","journal-title":"Microbiol. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2145","DOI":"10.1093\/jac\/dkx146","article-title":"Global epidemiology of CTX-M \u03b2-lactamases: Temporal and geographical shifts in genotype","volume":"72","author":"Bevan","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3111","DOI":"10.1093\/jac\/dkz304","article-title":"Emergence of the C1-M27 cluster in ST131 Escherichia coli from companion animals in France","volume":"74","author":"Melo","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"885","DOI":"10.3201\/eid2305.161865","article-title":"CTX-M-27-producing Escherichia coli of sequence type 131 and clade C1-M27, France","volume":"23","author":"Birgy","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1754","DOI":"10.3201\/eid2310.170938","article-title":"blaCTX-M-27-encoding Escherichia coli sequence type 131 lineage C1-M27 clone in clinical isolates, Germany","volume":"23","author":"Ghosh","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2973","DOI":"10.1093\/jac\/dky296","article-title":"Emergence of ESBL-producing Escherichia coli ST131-C1-M27 clade colonizing patients in Europe","volume":"73","author":"Merino","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1089\/mdr.2019.0351","article-title":"Occurrence of ESBL-producing Escherichia coli ST131, including the H30-Rx and C1-M27 subclones, among urban seagulls from the United Kingdom","volume":"26","author":"Zendri","year":"2020","journal-title":"Microb. Drug Resist."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/jac\/dkaa407","article-title":"Detection in livestock of the human pandemic Escherichia coli ST131 fimH30(R) clone carrying blaCTX-M-27","volume":"76","author":"Duggett","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1093\/jac\/dkv443","article-title":"An update on faecal carriage of ESBL-producing Enterobacteriaceae by Portuguese healthy humans: Detection of the H30 subclone of B2-ST131 Escherichia coli producing CTX-M-27","volume":"71","author":"Rodrigues","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"E497","DOI":"10.1111\/j.1469-0691.2012.03999.x","article-title":"Transmission dynamics of ESBL-producing Escherichia coli clones in rehabilitation wards at a tertiary care centre","volume":"18","author":"Adler","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"789280","DOI":"10.3389\/fmicb.2021.789280","article-title":"Whole-genome sequencing of extended-spectrum beta-lactamase-producing Escherichia coli from human infections in Finland revealed isolates belonging to internationally successful ST131-C1-M27 subclade but distinct from non-human sources","volume":"12","author":"Kurittu","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1128\/JCM.03502-13","article-title":"Rapid and specific detection, molecular epidemiology, and experimental virulence of the O16 subgroup within Escherichia coli sequence type 131","volume":"52","author":"Johnson","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"4736","DOI":"10.1128\/AAC.00641-13","article-title":"Association of fluoroquinolone resistance, virulence genes, and IncF plasmids with extended-spectrum-\u03b2-lactamase-producing Escherichia coli sequence type 131 (ST131) and ST405 clonal groups","volume":"57","author":"Matsumura","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"4997","DOI":"10.1128\/AAC.02824-14","article-title":"A new clone sweeps clean: The enigmatic emergence of Escherichia coli sequence type 131","volume":"58","author":"Banerjee","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"992","DOI":"10.1016\/j.vetmic.2012.10.029","article-title":"ESBL-producing uropathogenic Escherichia coli isolated from dogs and cats in Switzerland","volume":"162","author":"Huber","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1128\/AAC.05598-11","article-title":"Molecular characterization of extended-spectrum-\u03b2-lactamase-producing and plasmid-mediated AmpC \u03b2-lactamase-producing Escherichia coli isolated from stray dogs in South Korea","volume":"56","author":"Tamang","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.3201\/eid2106.141578","article-title":"Extensively drug-resistant New Delhi metallo-\u03b2-lactamase-encoding bacteria in the environment, Dhaka, Bangladesh, 2012","volume":"21","author":"Toleman","year":"2015","journal-title":"Emerg. Infect. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"282","DOI":"10.3855\/jidc.8138","article-title":"Characterization of ESBL-producing Escherichia coli recovered from companion dogs in Taiwan, China","volume":"11","author":"Li","year":"2017","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/j.meegid.2017.10.003","article-title":"Emergence of carbapenem resistant Escherichia coli isolates producing blaNDM and blaOXA-48-like carried on IncA\/C and IncL\/M plasmids at two Iranian university hospitals","volume":"55","author":"Solgi","year":"2017","journal-title":"Infect. Genet. Evol."},{"key":"ref_47","first-page":"2259","article-title":"Identification of KPC-2-producing Escherichia coli in a companion animal: A new challenge for veterinary clinicians","volume":"73","author":"Sellera","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.meegid.2018.09.009","article-title":"International high-risk clonal lineages of CTX-M-producing Escherichia coli F-ST648 in free-roaming cats, South America","volume":"66","author":"Fernandes","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1128\/microbiolspec.ARBA-0026-2017","article-title":"Antimicrobial resistance in Escherichia coli","volume":"6","author":"Poirel","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref_50","first-page":"3298","article-title":"Longitudinal study of ESBL Escherichia coli carriage on an organic broiler farm","volume":"73","author":"Veenman","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1089\/fpd.2019.2680","article-title":"Escherichia coli from commercial broiler and backyard chickens share sequence types, antimicrobial resistance profiles, and resistance genes with human extraintestinal pathogenic Escherichia coli","volume":"16","author":"Borges","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"101370","DOI":"10.1016\/j.psj.2021.101370","article-title":"Characterization of antimicrobial resistance in chicken-source phylogroup F Escherichia coli: Similar populations and resistance spectrums between E. coli recovered from chicken colibacillosis tissues and retail raw meats in Eastern China","volume":"100","author":"Wang","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1016\/j.ijantimicag.2010.09.012","article-title":"Emergence of clonal groups O1:HNM-D-ST59, O15:H1-D-ST393, O20:H34\/HNM-D-ST354, O25b:H4-B2-ST131 and ONT:H21,42-B1-ST101 among CTX-M-14-producing Escherichia coli clinical isolates in Galicia, northwest Spain","volume":"37","author":"Mora","year":"2011","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1743","DOI":"10.1099\/jmm.0.062729-0","article-title":"Co-selection may explain high rates of ciprofloxacin non-susceptible Escherichia coli from retail poultry reared without prior fluoroquinolone exposure","volume":"62","author":"Ingram","year":"2013","journal-title":"J. Med. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1904","DOI":"10.1099\/mic.0.000367","article-title":"Phylogenetic diversity, antimicrobial susceptibility and virulence characteristics of phylogroup F Escherichia coli in Australia","volume":"162","author":"Vangchhia","year":"2016","journal-title":"Microbiology"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"599031","DOI":"10.3389\/fmicb.2020.599031","article-title":"Corrigendum: Comprehensive molecular characterization of Escherichia coli isolates from urine samples of hospitalized patients in Rio de Janeiro, Brazil","volume":"11","author":"Campos","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"457","DOI":"10.1016\/j.ijantimicag.2016.03.019","article-title":"Circulation of clonal populations of fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST410 in humans and animals in Germany","volume":"47","author":"Falgenhauer","year":"2016","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"fiv155","DOI":"10.1093\/femsec\/fiv155","article-title":"Clonal spread and interspecies transmission of clinically relevant ESBL-producing Escherichia coli of ST410\u2014Another successful pandemic clone?","volume":"92","author":"Schaufler","year":"2016","journal-title":"FEMS Microbiol. Ecol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"e118","DOI":"10.1038\/emi.2016.117","article-title":"First identification of NDM-4-producing Escherichia coli ST410 in China","volume":"5","author":"Qin","year":"2016","journal-title":"Emerg. Microbes Infect."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Roer, L., Overballe-Petersen, S., Hansen, F., Sch\u00f8nning, K., Wang, M., R\u00f8der, B.L., Hansen, D.S., Justesen, U.S., Andersen, L.P., and Fulgsang-Damgaard, D. (2018). Escherichia coli sequence type 410 is causing new international high-risk clones. mSphere, 3.","DOI":"10.1128\/mSphere.00337-18"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.3201\/eid2209.160692","article-title":"Chromosomal locations of mcr-1 and blaCTX-M-15 in fluoroquinolone-resistant Escherichia coli ST410","volume":"22","author":"Falgenhauer","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1900071","DOI":"10.2807\/1560-7917.ES.2019.24.39.1900071","article-title":"Shedding of OXA-181 carbapenemase-producing Escherichia coli from companion animals after hospitalisation in Switzerland: An outbreak in 2018","volume":"24","author":"Nigg","year":"2019","journal-title":"Eurosurveillance"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Brilhante, M., Menezes, J., Belas, A., Feudi, C., Schwarz, S., Pomba, C., and Perreten, V. (2020). OXA-181-producing extraintestinal pathogenic Escherichia coli sequence type 410 isolated from a dog in Portugal. Antimicrob. Agents Chemother., 64.","DOI":"10.1128\/AAC.02298-19"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.1128\/AAC.01706-08","article-title":"Spread of bla(CTX-M-14) is driven mainly by IncK plasmids disseminated among Escherichia coli phylogroups A, B1, and D in Spain","volume":"53","author":"Valverde","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1016\/j.jgar.2016.02.001","article-title":"CTX-M-15 in combination with aac(6\u2032)-Ib-cr is the most prevalent mechanism of resistance both in Escherichia coli and Klebsiella pneumoniae, including K. pneumoniae ST258, in an ICU in Uruguay","volume":"6","author":"Bado","year":"2016","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Maluta, R.P., Logue, C.M., Casas, M.R., Meng, T., Guastalli, E.A., Rojas, T.C., Montelli, A.C., Sadatsune, T., de Carvalho Ramos, M., and Nolan, L.K. (2014). Overlapped sequence types (STs) and serogroups of avian pathogenic (APEC) and human extra-intestinal pathogenic (ExPEC) Escherichia coli isolated in Brazil. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0105016"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1264\/jsme2.ME17046","article-title":"Zoonotic potential and antibiotic resistance of Escherichia coli in neonatal calves in Uruguay","volume":"32","author":"Bado","year":"2017","journal-title":"Microbes Environ."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"3350","DOI":"10.1128\/aem.62.9.3350-3354.1996","article-title":"Confirmational identification of Escherichia coli, a comparison of genotypic and phenotypic assays for glutamate decarboxylase and beta-d-glucuronidase","volume":"62","author":"McDaniels","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_69","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2019). Performance Standards for Antimicrobial Susceptibility Testing\u201427th Edition. Approved Standard. M100-S29, Clinical and Laboratory Standards Institute."},{"key":"ref_70","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2020). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated from Animals\u20145th Edition Approved Standard, Clinical and Laboratory Standards Institute."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.diagmicrobio.2006.03.005","article-title":"Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin","volume":"56","author":"Pomba","year":"2006","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"3724","DOI":"10.1128\/AAC.47.12.3724-3732.2003","article-title":"Prevalence and molecular epidemiology of CTX-M Extended-Spectrum\u03b2-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Russian Hospitals","volume":"47","author":"Edelstein","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1093\/jac\/dki412","article-title":"Multiplex PCR for rapid detection of genes encoding CTX-M extended-spectrum (beta)-lactamases","volume":"57","author":"Woodford","year":"2006","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1128\/JCM.40.6.2153-2162.2002","article-title":"Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using Multiplex PCR","volume":"40","author":"Hanson","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"202","DOI":"10.1136\/vr.101978","article-title":"Risk factors for faecal colonisation with Escherichia coli producing extended-spectrum and plasmid-mediated AmpC \u03b2-lactamases in dogs","volume":"175","author":"Belas","year":"2014","journal-title":"Vet. Rec."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.diagmicrobio.2010.12.002","article-title":"Multiplex PCR for detection of acquired carbapenemase genes","volume":"70","author":"Poirel","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"3108","DOI":"10.1128\/JCM.01468-12","article-title":"Improved multiplex PCR strategy for rapid assignment of the four major Escherichia coli phylogenetic groups","volume":"50","author":"Doumith","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1099\/mic.0.2007\/013086-0","article-title":"A subset of mucosa associated Escherichia coli isolates from patients with colon cancer, but not Crohn\u2019s disease, share pathogenicity islands with urinary pathogenic E. coli","volume":"154","author":"Bronowski","year":"2008","journal-title":"Microbiology"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1007\/0-306-46840-9_40","article-title":"Detection of virulence factors in uropathogenic Escherichia coli isolated from humans, dogs and cats in Portugal","volume":"485","author":"Correia","year":"2002","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"6385","DOI":"10.1128\/AAC.01604-13","article-title":"Molecular epidemiology of Escherichia coli Sequence Type 131 and its H30 and H30-Rx subclones among Extended-Spectrum-\u03b2-Lactamase-positive and -negative E. coli clinical isolates from the Chicago region, 2007 to 2010","volume":"57","author":"Banerjee","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1093\/cid\/cit503","article-title":"Escherichia coli sequence type 131 (ST131) subclone H30 as an emergent multidrug-resistant pathogen among US veterans","volume":"57","author":"Colpan","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1111\/j.1365-2958.2006.05172.x","article-title":"Sex and virulence in Escherichia coli: An evolutionary perspective","volume":"60","author":"Wirth","year":"2006","journal-title":"Mol. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/5\/559\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T22:58:45Z","timestamp":1760137125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/5\/559"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,22]]},"references-count":83,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["antibiotics11050559"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11050559","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,22]]}}}