{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:28:25Z","timestamp":1782779305692,"version":"3.54.5"},"reference-count":41,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>A chair-side test (CST) for five periodontal pathogens (Aggregatibacter actinomycetemcomitans, A.a.; Porphyromonas gingivalis, P.g.; Prevotella intermedia, P.i.; Treponema denticola, T.d.; Tannerella forsythia, T.f.) was compared with qPCR in a previous clinical study on 100 periodontitis patients at first diagnosis (T0). Following non-surgical treatment alone (SRP) or in combination with systemic or local antibiotics, 74 patients (57.4 \u00b1 13.5 years) were again tested at the same sites from 14 to 24 months after T0. Bacterial elimination (%; compared to T0) was determined for each single species and compared between both test systems. In all patients, all five pathogens could not be fully eliminated regardless of therapy or test method. Tested with CST, the mean elimination ranged from 90% for SRP + Amoxicillin\/Metronidazole to 59.13% for SRP only. The corresponding qPCR values were 30% and 29.6%. Only A.a. was eradicated in 100% by SRP + Amoxicillin\/Metronidazole tested by CST, and it was 80% when qPCR was the test method. CST agreed with qPCR in 98.7% in the detection of A.a., and 74.3%, 78.4%, 73.0%, and 48.7% for P.g., P.i., T.d., and T.f., respectively. Neither conventional treatment nor the additional use of antibiotics\u2014even with the correct indication\u2014could completely eradicate the tested pathogens or prevent pocket reinfection.<\/jats:p>","DOI":"10.3390\/antibiotics11050577","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T11:45:55Z","timestamp":1650973555000},"page":"577","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Influence of Anti-Infective Periodontal Therapy on Subgingival Microbiota Evaluated by Chair-Side Test Compared to qPCR\u2014A Clinical Follow-Up Study"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6432-2151","authenticated-orcid":false,"given":"Oliver","family":"Laugisch","sequence":"first","affiliation":[{"name":"Department of Periodontology and Peri-Implant Diseases, Philipps-University, 35039 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten M.","family":"Auschill","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Peri-Implant Diseases, Philipps-University, 35039 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Tumbrink","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Peri-Implant Diseases, Philipps-University, 35039 Marburg, Germany"},{"name":"Private Practice, 48324 Sendenhorst, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2836-5477","authenticated-orcid":false,"given":"Anton","family":"Sculean","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dental Medicine, University of Bern, 3010 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1453-0697","authenticated-orcid":false,"given":"Nicole B.","family":"Arweiler","sequence":"additional","affiliation":[{"name":"Department of Periodontology and Peri-Implant Diseases, Philipps-University, 35039 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1111\/j.1600-051X.1998.tb02419.x","article-title":"Microbial complexes in subgingival plaque","volume":"25","author":"Socransky","year":"1998","journal-title":"J. Clin. Periodontol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1111\/jcpe.13290","article-title":"Treatment of stage I-III periodontitis-The EFP S3 level clinical practice guideline","volume":"47","author":"Sanz","year":"2020","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1111\/prd.12344","article-title":"Clinical and public health implications of periodontal and systemic diseases: An overview","volume":"83","author":"Genco","year":"2020","journal-title":"Periodontol. 2000"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1007\/978-3-319-31248-4_4","article-title":"The Oral Microbiota","volume":"902","author":"Arweiler","year":"2016","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/JPER.19-0180","article-title":"Clinical evaluation of a newly developed chairside test to determine periodontal pathogens","volume":"91","author":"Arweiler","year":"2020","journal-title":"J. Periodontol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1902\/jop.2012.120281","article-title":"Are there specific benefits of amoxicillin plus metronidazole in Aggregatibacter actinomycetemcomitans-associated periodontitis? Double-masked, randomized clinical trial of efficacy and safety","volume":"84","author":"Mombelli","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1253","DOI":"10.1902\/jop.2017.170286","article-title":"Microbiologic Response to Periodontal Therapy and Multivariable Prediction of Clinical Outcome","volume":"88","author":"Mombelli","year":"2017","journal-title":"J. Periodontol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1111\/jcpe.13164","article-title":"Empirical or microbiologically guided systemic antimicrobials as adjuncts to non-surgical periodontal therapy? A systematic review","volume":"46","author":"Nibali","year":"2019","journal-title":"J. Clin. Periodontol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1007\/s00784-016-1722-6","article-title":"Trends in antibiotic use and microbial diagnostics in periodontal treatment: Comparing surveys of German dentists in a ten-year period","volume":"20","author":"Falkenstein","year":"2016","journal-title":"Clin. Oral Investig."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3031","DOI":"10.1007\/s00784-018-2392-3","article-title":"Microbiological analysis and the outcomes of periodontal treatment with or without adjunctive systemic antibiotics-a retrospective study","volume":"22","author":"Eick","year":"2018","journal-title":"Clin. Oral Investig."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1080","DOI":"10.1111\/jcpe.12299","article-title":"Impact of baseline microbiological status on clinical outcomes in generalized aggressive periodontitis patients treated with or without adjunctive amoxicillin and metronidazole: An exploratory analysis from a randomized controlled clinical trial","volume":"41","author":"Guerrero","year":"2014","journal-title":"J. Clin. Periodontol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.archoralbio.2019.05.023","article-title":"Applications of the oral microbiome in personalized dentistry","volume":"104","author":"Belibasakis","year":"2019","journal-title":"Arch. Oral Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"258","DOI":"10.3109\/00016357.2014.920106","article-title":"The effect of metronidazole on the presence of P. gingivalis and T. forsythia at 3 and 12 months after different periodontal treatment strategies evaluated in a randomized, clinical trial","volume":"73","author":"Preus","year":"2015","journal-title":"Acta Odontol. Scand."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1111\/jcpe.12559","article-title":"Non-surgical periodontal treatment in conjunction with 3 or 7 days systemic administration of amoxicillin and metronidazole in severe chronic periodontitis patients. A placebo-controlled randomized clinical study","volume":"43","author":"Cosgarea","year":"2016","journal-title":"J. Clin. Periodontol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1034\/j.1600-0757.2002.2801123.x","article-title":"Effective, safe, practical and affordable periodontal antimicrobial therapy: Where are we going, and are we there yet?","volume":"28","author":"Slots","year":"2002","journal-title":"Periodontol. 2000"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1902\/jop.2012.120040","article-title":"The clinical effect of scaling and root planing and the concomitant administration of systemic amoxicillin and metronidazole: A systematic review","volume":"84","author":"Zandbergen","year":"2013","journal-title":"J. Periodontol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3770","DOI":"10.1128\/JB.183.12.3770-3783.2001","article-title":"Bacterial diversity in human subgingival plaque","volume":"183","author":"Paster","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1111\/j.1600-051X.2005.00740.x","article-title":"Comparison of culture and real-time PCR for detection and quantification of five putative periodontopathogenic bacteria in subgingival plaque samples","volume":"32","author":"Koltzscher","year":"2005","journal-title":"J. Clin. Periodontol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3109\/00016350903514848","article-title":"Comparison of two different microbiological test kits for detection of periodontal pathogens","volume":"68","author":"Cosgarea","year":"2010","journal-title":"Acta Odontol. Scand."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.diagmicrobio.2010.08.017","article-title":"Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment","volume":"69","author":"Eick","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"253","DOI":"10.1111\/j.2041-1014.2011.00615.x","article-title":"Live\/dead real-time polymerase chain reaction to assess new therapies against dental plaque-related pathologies","volume":"26","author":"Loozen","year":"2011","journal-title":"Mol. Oral Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1111\/jre.12052","article-title":"Assessment of viable periodontal pathogens by reverse transcription quantitative polymerase chain reaction","volume":"48","author":"Polonyi","year":"2013","journal-title":"J. Periodontal. Res."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2045","DOI":"10.1007\/s00784-015-1418-3","article-title":"Inter- and intra-test agreement of three commercially available molecular diagnostic tests for the identification of periodontal pathogens","volume":"19","author":"Untch","year":"2015","journal-title":"Clin. Oral Investig."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1111\/j.1600-0757.1996.tb00075.x","article-title":"Compliance and its role in periodontal therapy","volume":"12","author":"Wilson","year":"1996","journal-title":"Periodontol. 2000"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.1177\/00220345950740080501","article-title":"Full- vs. partial-mouth disinfection in the treatment of periodontal infections: Short-term clinical and microbiological observations","volume":"74","author":"Quirynen","year":"1995","journal-title":"J. Dent. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1111\/jcpe.12117","article-title":"Effect of periodontal therapy on the subgingival microbiota over a 2-year monitoring period. I. Overall effect and kinetics of change","volume":"40","author":"Socransky","year":"2013","journal-title":"J. Clin. Periodontol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"106","DOI":"10.4103\/0972-124X.70829","article-title":"Compliance to recall visits by patients with periodontitis-Is the practitioner responsible?","volume":"14","author":"Fenol","year":"2010","journal-title":"J. Indian Soc. Periodontol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"976","DOI":"10.1902\/jop.1996.67.10.976","article-title":"Compliance with supportive periodontal therapy and its relation to the bleeding index","volume":"67","author":"Novaes","year":"1996","journal-title":"J. Periodontol."},{"key":"ref_29","first-page":"e42","article-title":"Compliance with periodontal maintenance at the University of Pittsburgh: Retrospective analysis of 315 cases","volume":"58","author":"Famili","year":"2010","journal-title":"Gen. Dent."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1902\/jop.1995.66.2.145","article-title":"Compliance with supportive periodontal treatment in private periodontal practice. A 14-year retrospective study","volume":"66","author":"Demetriou","year":"1995","journal-title":"J. Periodontol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1111\/idh.12290","article-title":"Supportive periodontal treatment: Pocket depth changes and tooth loss","volume":"16","author":"Slot","year":"2018","journal-title":"Int. J. Dent. Hyg."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1765","DOI":"10.1007\/s00784-015-1683-1","article-title":"Nonsurgical therapy of chronic periodontitis with adjunctive systemic azithromycin or amoxicillin\/metronidazole","volume":"20","author":"Jentsch","year":"2016","journal-title":"Clin. Oral Investig."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.ejphar.2013.02.044","article-title":"Azithromycin: A new concept in adjuvant treatment of periodontitis","volume":"705","author":"Muniz","year":"2013","journal-title":"Eur. J. Pharmacol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1111\/j.1600-051X.2010.01607.x","article-title":"Azithromycin as an adjunct to scaling and root planing in the treatment of Porphyromonas gingivalis-associated periodontitis: A pilot study","volume":"37","author":"Oteo","year":"2010","journal-title":"J. Clin. Periodontol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Butera, A., Gallo, S., Pascadopoli, M., Maiorani, C., Milone, A., Alovisi, M., and Scribante, A. (2022). Paraprobiotics in Non-Surgical Periodontal Therapy: Clinical and Microbiological Aspects in a 6-Month Follow-Up Domiciliary Protocol for Oral Hygiene. Microorganisms, 10.","DOI":"10.3390\/microorganisms10020337"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Butera, A., Gallo, S., Pascadopoli, M., Taccardi, D., and Scribante, A. (2022). Home Oral Care of Periodontal Patients Using Antimicrobial Gel with Postbiotics, Lactoferrin, and Aloe Barbadensis Leaf Juice Powder vs. Conventional Chlorhexidine Gel: A Split-Mouth Randomized Clinical Trial. Antibiotics, 11.","DOI":"10.3390\/antibiotics11010118"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Butera, A., Gallo, S., Maiorani, C., Molino, D., Chiesa, A., Preda, C., Esposito, F., and Scribante, A. (2020). Probiotic Alternative to Chlorhexidine in Periodontal Therapy: Evaluation of Clinical and Microbiological Parameters. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010069"},{"key":"ref_38","unstructured":"Meier, K. (2007). Guidance for Industry and FDA Staff-Statistical Guidance on Reporting Results from Studies Evaluating Diagnostic Tests."},{"key":"ref_39","unstructured":"Garrett, P.E., Lasky, F.D., Meier, K.L., and Clark, L.W. (2008). User Protocol for Evaluating of Qualitative Test Performance: Approved Guideline, Clinical and Laboratory Standars Institute. [2nd ed.]."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1111\/j.1600-0757.2005.00116.x","article-title":"Microbiological diagnostics in periodontics: Biological significance and clinical validity","volume":"39","author":"Winkel","year":"2005","journal-title":"Periodontol. 2000"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1034\/j.1600-051x.2002.290204.x","article-title":"Non-surgical periodontal therapy with adjunctive topical doxycycline: A double-blind randomized controlled multicenter study","volume":"29","author":"Eickholz","year":"2002","journal-title":"J. Clin. Periodontol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/5\/577\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:01:06Z","timestamp":1760137266000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/5\/577"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,26]]},"references-count":41,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2022,5]]}},"alternative-id":["antibiotics11050577"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11050577","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,26]]}}}