{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T18:45:38Z","timestamp":1770144338233,"version":"3.49.0"},"reference-count":14,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T00:00:00Z","timestamp":1650931200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: The direct identification of uropathogens from urine samples, in combination with the rapid detection of resistance, would allow early adjustment of empirical antimicrobial treatment. Methods: Two hundred and ninety-eight urine samples processed between 1 June and 31 December 2020, selected with flow cytometry, with direct identification by MALDI-TOF mass spectrometry, and rapid detection of extended-spectrum beta-lactamase (ESBL) and carbapenemases-producing strains by lateral flow were analyzed. Results: The positive predictive value of the direct identification of the 86 samples that met the flow cytometry criterion (&gt;5000 bacteria\/\u00b5L) was 96.4%. Reliable direct identification was obtained in 14 of the 27 (51.8%) urinary source bacteraemias. There was 100% agreement between the lateral flow and antibiogram in the detection of ESBL and carbapenemases. Conclusion: the protocol for the direct identification and rapid detection of ESBL and carbapenemases-producing strains from urine samples is a reliable and useful tool.<\/jats:p>","DOI":"10.3390\/antibiotics11050582","type":"journal-article","created":{"date-parts":[[2022,4,26]],"date-time":"2022-04-26T11:45:55Z","timestamp":1650973555000},"page":"582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Implementation of a New Protocol for Direct Identification from Urine in the Routine Microbiological Diagnosis"],"prefix":"10.3390","volume":"11","author":[{"given":"Yuliya","family":"Zboromyrska","sequence":"first","affiliation":[{"name":"Microbiology Department, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ver\u00f3nica","family":"Rico","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cristina","family":"Pitart","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Clinic, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana Jos\u00e9","family":"Fern\u00e1ndez-Pittol","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9374-0811","authenticated-orcid":false,"given":"\u00c1lex","family":"Soriano","sequence":"additional","affiliation":[{"name":"Infectious Diseases Department, Hospital Clinic, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jordi","family":"Bosch","sequence":"additional","affiliation":[{"name":"Microbiology Department, Hospital Clinic, 08036 Barcelona, Spain"},{"name":"Barcelona Institute for Global Health (ISGlobal), Hospital Clinic, University of Barcelona, 08036 Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,4,26]]},"reference":[{"key":"ref_1","first-page":"145","article-title":"Monitoring the antimicrobial susceptibility of Gram-negative organisms involved in intraabdominal and urinary tract infections recovered during the SMART study (Spain, 2016 and 2017)","volume":"32","author":"Loza","year":"2019","journal-title":"Rev. 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