{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T13:03:57Z","timestamp":1781615037802,"version":"3.54.5"},"reference-count":78,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T00:00:00Z","timestamp":1651104000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Banat University of Agricultural Sciences and Veterinary Medicine","award":["Increasing the impact of excellence research on the capacity for innovation and technology transfer within USAMVB Timi\u0219oara\" code 6PFE, submitted in the competition Program 1 - Development of the national system of research - development, Subprogram 1.2 -"],"award-info":[{"award-number":["Increasing the impact of excellence research on the capacity for innovation and technology transfer within USAMVB Timi\u0219oara\" code 6PFE, submitted in the competition Program 1 - Development of the national system of research - development, Subprogram 1.2 -"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>This study aimed to investigate the antioxidant profile and the antimicrobial activity of four different types of monofloral honey (manuka (MH), brassica rapeseed (BH), acacia (AH), and linden honey (LH)) against some bacterial\/fungal ATCC strains and some multidrug-resistant strains isolated from chronic otitis in dogs. For the characterisation of the antioxidant profile of each honey, we extracted the honey samples by hydroalcoholic extraction and analysed them in terms of total polyphenols (TPC), total flavonoids (TFC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) using the spectrophotometric method. The antimicrobial activity was determined using the microdilution method at concentrations of 10%, 15%, and 20%, with the results expressed in OD (optical density) calculated as BIR% (bacterial inhibition rate)\/MIR% (mycelial inhibition rate). The antioxidant characterisation of the analysed honey samples showed the highest antioxidant activity and concentrations of TPC and TFC in MH, followed by LH. MH was proven to be the most effective on most clinical isolates concerning the antimicrobial activity in comparison with BH, AH, and LH. Except for B. cepacia and P. vulgaris, all the clinical isolates were sensitive to the antibacterial activity of honey. Regarding the ATCC strains, MH 10% was the most effective in inhibiting all the strains tested except for P. aeruginosa. In conclusion, the efficacy classification in our study was MH &gt; BH &gt; AH &gt; LH.<\/jats:p>","DOI":"10.3390\/antibiotics11050595","type":"journal-article","created":{"date-parts":[[2022,4,28]],"date-time":"2022-04-28T12:06:01Z","timestamp":1651147561000},"page":"595","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Diversity of Monofloral Honey Based on the Antimicrobial and Antioxidant Potential"],"prefix":"10.3390","volume":"11","author":[{"given":"Anca","family":"Hulea","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1481-6954","authenticated-orcid":false,"given":"Diana","family":"Obi\u0219tioiu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7621-402X","authenticated-orcid":false,"given":"Ileana","family":"Cocan","sequence":"additional","affiliation":[{"name":"Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4641-7365","authenticated-orcid":false,"given":"Ersilia","family":"Alexa","sequence":"additional","affiliation":[{"name":"Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9765-8498","authenticated-orcid":false,"given":"Monica","family":"Negrea","sequence":"additional","affiliation":[{"name":"Faculty of Food Engineering, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, 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Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luminita","family":"Costinar","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ionica","family":"Iancu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Banat University of Agricultural Sciences and Veterinary Medicine \u201cKing Michael I of Romania\u201d Timisoara, Calea Aradului No. 119, 300645 Timisoara, 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