{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T03:13:06Z","timestamp":1762053186029,"version":"build-2065373602"},"reference-count":31,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2022,6,8]],"date-time":"2022-06-08T00:00:00Z","timestamp":1654646400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office for Research of the WesternU College of Veterinary Medicine"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>An interdisciplinary approach to antimicrobial resistance (AMR) is essential to effectively address what is projected to soon become a public health disaster. Veterinary medicine accounts for a majority of antimicrobial use, and mainly in support of industrial food animal production (IFAP), which has significant exposure implications for human and nonhuman animals. Companion dogs live in close proximity to humans and share environmental exposures, including food sources. This study aimed to elucidate the AMR-gene presence in microorganisms recovered from urine from clinically healthy dogs to highlight public health considerations in the context of a species-spanning framework. Urine was collected through cystocentesis from 50 companion dogs in Southern California, and microbial DNA was analyzed using next-generation sequencing. Thirteen AMR genes in urine from 48% of the dogs {n=24} were detected. The most common AMR genes were aph(3\u2032)Ia, and ermB, which confer resistance to aminoglycosides and MLS (macrolides, lincosamides, streptogramins) antibiotics, respectively. Antibiotic-resistance profiles based on the AMR genes detected, and the intrinsic resistance profiles of bacterial species, were inferred in 24% of the samples {n=12} for 57 species, with most belonging to Streptococcus, Staphylococcus, and Corynebacterium genera. The presence of AMR genes that confer resistance to medically important antibiotics suggests that dogs may serve as reservoirs of clinically relevant resistomes, which is likely rooted in excessive IFAP antimicrobial use.<\/jats:p>","DOI":"10.3390\/antibiotics11060780","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T10:49:14Z","timestamp":1654771754000},"page":"780","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["The Urinary Resistome of Clinically Healthy Companion Dogs: Potential One Health Implications"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-6218","authenticated-orcid":false,"given":"Tonatiuh","family":"Melgarejo","sequence":"first","affiliation":[{"name":"Veterinary Clinical Center, College of Veterinary Medicine, Western University of Health Sciences, 611 E. Second Street, Pomona, CA 91766, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan","family":"Sharp","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Center, College of Veterinary Medicine, Western University of Health Sciences, 611 E. Second Street, Pomona, CA 91766, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4865-7170","authenticated-orcid":false,"given":"Janina","family":"Krumbeck","sequence":"additional","affiliation":[{"name":"MiDOG LLC, 14672 Bentley Cir, Tustin, CA 92780, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guangxi","family":"Wu","sequence":"additional","affiliation":[{"name":"MiDOG LLC, 14672 Bentley Cir, Tustin, CA 92780, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Center, College of Veterinary Medicine, Western University of Health Sciences, 611 E. Second Street, Pomona, CA 91766, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5749-4852","authenticated-orcid":false,"given":"Annika","family":"Linde","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Center, College of Veterinary Medicine, Western University of Health Sciences, 611 E. Second Street, Pomona, CA 91766, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,8]]},"reference":[{"key":"ref_1","unstructured":"O\u2019Neil, J. (2021, August 10). Tackling Drug-Resistant Infections Globally: Final Report and Recommendations. The Review on Antimicrobial Resistance. United Kingdom. Available online: https:\/\/amr-review.org\/sites\/default\/files\/160518_Final%20paper_with%20cover.pdf."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Centers for Disease Control and Prevention (2021, August 10). 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