{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:36:37Z","timestamp":1790750197869,"version":"4.1.0"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Internal Collaborative Project of Pasteur Institute of Tunis (PCI)","award":["PCI-23"],"award-info":[{"award-number":["PCI-23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Antibiotic resistance in foodborne pathogens is an emergent global health concern. The objectives of this study were to assess antimicrobial resistance (AMR) in Campylobacter isolates from chicken carcasses and to investigate the AMR molecular mechanisms as well as the presence of virulence determinants. The study was performed on 257 samples collected from abattoirs and retail shops in northeastern Tunisia. Forty-eight Campylobacter isolates were recovered and identified as C. jejuni (n = 33) and C. coli (n = 15). Antibiotic resistance was tested against eight antibiotics and high resistance rates were observed against tetracycline (100%), erythromycin (97.9%), ciprofloxacin (73%), nalidixic acid (85.4%), ampicillin (83.3%), amoxicillin\/clavulanic acid (22.9%), chloramphenicol (75%), and gentamicin (27.1%). All isolates were multidrug-resistant, and 22 resistance patterns were found. All isolates were screened for AMR genes (tet(O), tet(A), tet(B), tet(L), cmeB, ermB, blaOXA-61, and aphA-3), and for point mutations in gyrA (C257T substitution) and 23SrRNA (A2075G\/A2074C) genes. All screened AMR genes, as well as the C257T and the A2075G mutations, were detected. The virulence genotypes were also determined, and all isolates carried the motility (flaA) and invasion (cadF) genes. Most of them also harbored the cdtA, cdtB, and cdtC genes, encoding the Campylobacter toxin. The screening of the cgtB and the wlaN genes, involved in Guillain-Barr\u00e9 Syndrome expression, revealed the presence of the cgtB in 21.2% of C. jejuni strains, whereas none of them carried the wlaN gene. Our findings highlight the emergence of Campylobacter strains simultaneously harboring several virulence and AMR determinants, which emphasizes the risk of transmission of MDR strains to humans via the food chain. Hence, controlling the dissemination of foodborne pathogens \u201cfrom the farm to the fork\u201d as well as restricting the use of antimicrobials in husbandry are mandatory to prevent the risk for consumers and to mitigate the dissemination of MDR pathogens.<\/jats:p>","DOI":"10.3390\/antibiotics11070830","type":"journal-article","created":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T21:55:19Z","timestamp":1655848519000},"page":"830","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Virulence Profiling, Multidrug Resistance and Molecular Mechanisms of Campylobacter Strains from Chicken Carcasses in Tunisia"],"prefix":"10.3390","volume":"11","author":[{"given":"Awatef","family":"B\u00e9jaoui","sequence":"first","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manel","family":"Gharbi","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarra","family":"Bitri","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorsaf","family":"Nasraoui","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wassim","family":"Ben Aziza","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kais","family":"Ghedira","sequence":"additional","affiliation":[{"name":"Laboratory of Bioinformatics, Biomathematics and Biostatistics, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryem","family":"Rfaik","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Marzougui","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdeljelil","family":"Ghram","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abderrazek","family":"Maaroufi","sequence":"additional","affiliation":[{"name":"Laboratory of Epidemiology and Veterinary Microbiology, Group of Bacteriology and Biotechnology Development, Institut Pasteur de Tunis, University of Tunis El Manar (UTM), Tunis 1002, Tunisia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1128\/CMR.00006-15","article-title":"Global Epidemiology of Campylobacter Infection","volume":"28","author":"Kaakoush","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9088","DOI":"10.1371\/journal.pone.0219088","article-title":"Molecular characterization of Campylobacter causing human clinical infection using whole-genome sequencing: Virulence, antimicrobial resistance and phylogeny in Ireland","volume":"14","author":"Redondo","year":"2019","journal-title":"PLoS ONE"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"852195","DOI":"10.1155\/2013\/852195","article-title":"Role of Campylobacter jejuni Infection in the Pathogenesis of Guillain-Barr\u00e9 Syndrome: An Update","volume":"2013","author":"Nyati","year":"2013","journal-title":"BioMed Res. Int."},{"key":"ref_4","unstructured":"World Health Organization (WHO) (2022, June 11). \u00a0Campylobacter. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/campylobacter."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1016\/j.mimet.2016.06.028","article-title":"Influence of enrichment and isolation media on the detection of Campylobacter spp. in naturally contaminated chicken samples","volume":"128","author":"Laisney","year":"2016","journal-title":"J. Microbiol. Methods"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"215","DOI":"10.1016\/bs.afnr.2018.04.005","article-title":"Campylobacter in the Food Chain","volume":"86","author":"Melero","year":"2018","journal-title":"Adv. Food Nutr. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.3389\/fmicb.2018.02092","article-title":"Prevalence and Characteristics of Campylobacter Throughout the Slaughter Process of Different Broiler Batches","volume":"9","author":"Zhang","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.fm.2014.11.017","article-title":"Campylobacter virulence and survival factors","volume":"48","author":"Bolton","year":"2015","journal-title":"Food Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1089\/mdr.2013.0110","article-title":"Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli from Poultry in Italy","volume":"20","author":"Giacomelli","year":"2014","journal-title":"Microb. Drug Resist."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Machowska, A., and Lundborg, C.S. (2018). Drivers of Irrational Use of Antibiotics in Europe. Int. J. Environ. Res. Public Health, 16.","DOI":"10.3390\/ijerph16010027"},{"key":"ref_11","unstructured":"WHO (2022, June 11). Tunisia: National Action Plan to Fight against Antimicrobial Resistance. Available online: https:\/\/www.who.int\/publications\/m\/item\/tunisia-national-action-plan-to-fight-against-antimicrobial-resistance."},{"key":"ref_12","unstructured":"WHO Regional Office for the Eastern Mediterranean (2022, June 11). Antimicrobial Resistance in the Region. Available online: http:\/\/www.emro.who.int\/health-topics\/drug-resistance\/regional-situation.html."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Di Giannatale, E., Calistri, P., Di Donato, G., Decastelli, L., Goffredo, E., Adriano, D., Mancini, M.E., Galleggiante, A., Neri, D., and Antoci, S. (2019). Thermotolerant Campylobacter spp. in chicken and bovine meat in Italy: Prevalence, level of contamination and molecular characterization of isolates. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0225957"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Kim, J., Park, H., Kim, J., Kim, J.H., Jung, J.I., Cho, S., Ryu, S., and Jeon, B. (2019). Comparative Analysis of Aerotolerance, Antibiotic Resistance, and Virulence Gene Prevalence in Campylobacter jejuni Isolates from Retail Raw Chicken and Duck Meat in South Korea. Microorganisms, 7.","DOI":"10.3390\/microorganisms7100433"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"7943786","DOI":"10.1155\/2018\/7943786","article-title":"Prevalence and Antibiotic Resistance Patterns of Campylobacter spp. Isolated from Broiler Chickens in the North of Tunisia","volume":"2018","author":"Gharbi","year":"2018","journal-title":"BioMed. Res. Int."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.mimet.2006.12.011","article-title":"Efficacy of supplemented buffered peptone water for the isolation of Campylobacter jejuni and C. coli from broiler retail products","volume":"69","author":"Oyarzabal","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ijfoodmicro.2009.11.013","article-title":"Survival of Campylobacter spp. in poultry meat preparations subjected to freezing, refrigeration, minor salt concentration, and heat treatment","volume":"137","author":"Sampers","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.ijfoodmicro.2017.04.019","article-title":"Epidemiology of antimicrobial resistant Campylobacter spp. isolated from retail meats in Canada","volume":"253","author":"Taboada","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_19","first-page":"762","article-title":"Survival of Campylobacter jejuni and Campylobacter coli water isolates in lake and well water","volume":"126","author":"Nilsson","year":"2018","journal-title":"J. Pathol. Microbiol. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"295","DOI":"10.3389\/fmicb.2015.00295","article-title":"Impact of oxidative stress defense on bacterial survival and morphological change in Campylobacter jejuni under aerobic conditions","volume":"6","author":"Oh","year":"2015","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/j.ijfoodmicro.2012.01.006","article-title":"Prevalence, antibiotic resistance and RAPD typing of Campylobacter species isolated from ducks, their rearing and processing environments in Penang, Malaysia","volume":"154","author":"Adzitey","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.vetmic.2009.12.035","article-title":"Prevalence and antimicrobial resistance of Campylobacter isolates in broilers from China","volume":"144","author":"Chen","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_23","first-page":"59","article-title":"Molecular epidemiology of Campylobacter jejuni isolates from the broiler production chain: First report of MLST profiles in Argentina","volume":"53","author":"Zbrun","year":"2021","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4091856","DOI":"10.1155\/2017\/4091856","article-title":"Antibiotic Resistance of Campylobacter Recovered from Faeces and Carcasses of Healthy Livestock","volume":"2017","author":"Karikari","year":"2017","journal-title":"BioMed. Res. Int."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"592496","DOI":"10.3389\/fmicb.2020.592496","article-title":"Antibiotic Resistance Profiles and Molecular Mechanisms of Campylobacter from Chicken and Pig in China","volume":"11","author":"Tang","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1016\/j.micinf.2005.12.032","article-title":"Mechanisms of fluoroquinolone and macrolide resistance in Campylobacter spp.","volume":"8","author":"Payot","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1111\/j.1574-6968.2002.tb11007.x","article-title":"Identification and molecular characterisation of CmeB, a Campylobacter jejuni multidrug efflux pump","volume":"206","author":"Pumbwe","year":"2002","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.foodcont.2013.12.016","article-title":"Frequency of contamination and antimicrobial resistance of thermotolerant Campylobacter isolated from some broiler farms and slaughterhouses in the region of Algiers","volume":"40","author":"Messad","year":"2014","journal-title":"Food Control"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wysok, B., Wojtacka, J., Wiszniewska-\u0141aszczych, A., and Szteyn, J. (2020). Antimicrobial Resistance and Virulence Properties of Campylobacter spp. Originating from Domestic Geese in Poland. Animals, 10.","DOI":"10.3390\/ani10040742"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.3389\/fmicb.2018.01014","article-title":"Genetic Basis and Clonal Population Structure of Antibiotic Resistance in Campylobacter jejuni Isolated from Broiler Carcasses in Belgium","volume":"9","author":"Elhadidy","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"18216","DOI":"10.1038\/s41598-019-54620-1","article-title":"Acquisition of fluoroquinolone resistance leads to increased biofilm formation and pathogenicity in Campylobacter jejuni","volume":"9","author":"Whelan","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"666","DOI":"10.1093\/jac\/dkv382","article-title":"Species shift and multidrug resistance of Campylobacter from chicken and swine, China, 2008\u20132014","volume":"71","author":"Wang","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"82","DOI":"10.7883\/yoken.JJID.2008.82","article-title":"Trends in antibiotic sensitivity pattern and molecular detection of tet(O)-mediated tetracycline resistance in Campylobacter jejuni isolates from human and poultry sources","volume":"61","author":"Mazi","year":"2008","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1186\/s13099-016-0121-5","article-title":"Antimicrobial resistance of Campylobacter isolates from small scale and backyard chicken in Kenya","volume":"8","author":"Nguyen","year":"2016","journal-title":"Gut Pathog."},{"key":"ref_35","first-page":"e12129","article-title":"Tetracycline Resistance Genes in Campylobacter jejuni and C. coli Isolated from Poultry Carcasses","volume":"7","author":"Hachesoo","year":"2014","journal-title":"Jundishapur J. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Francesco, A., Salvatore, D., Sakhria, S., Catelli, E., Lupini, C., Abbassi, M., Bessoussa, G., Ben Yahia, S., and Ben Chehida, N. (2021). High Frequency and Diversity of Tetracycline Resistance Genes in the Microbiota of Broiler Chickens in Tunisia. Animals, 11.","DOI":"10.3390\/ani11020377"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"596","DOI":"10.1093\/jac\/dkaa483","article-title":"Global distribution, dissemination and overexpression of potent multidrug efflux pump RE-CmeABC in Campylobacter jejuni","volume":"76","author":"Yao","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_38","first-page":"2126","article-title":"Status of antimicrobial resistance in clinical isolates of Campylobacter jejuni and Campylobacter coli in Australia","volume":"82","author":"Wallace","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.1128\/AAC.01655-08","article-title":"\u03b2-Lactamase-Mediated \u03b2-Lactam Resistance in Campylobacter Species: Prevalence of Cj0299 (blaOXA-61) and Evidence for a Novel \u03b2-Lactamase in C. jejuni","volume":"53","author":"Griggs","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"108656","DOI":"10.1016\/j.ijfoodmicro.2020.108656","article-title":"Prevalence, antibiotic resistance and genotypes of Campylobacter jejuni and  Campylobacter coli isolated from chickens in Irbid governorate, Jordan","volume":"327","author":"Alaboudi","year":"2020","journal-title":"Int. J. Food Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.foodcont.2017.03.048","article-title":"Prevalence and antimicrobial resistance patterns of Campylobacter spp. isolated from retail meat in Lahore, Pakistan","volume":"80","author":"Nisar","year":"2017","journal-title":"Food Control"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e00390-18","DOI":"10.1128\/JCM.00390-18","article-title":"Whole-Genome Sequence Analysis of Multidrug-Resistant Campylobacter Isolates: A Focus on Aminoglycoside Resistance Determinants","volume":"56","author":"Fabre","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA) (2014). Scientific Opinion on Chloramphenicol in food and feed. EFSA J., 12, 3907.","DOI":"10.2903\/j.efsa.2014.3907"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1089\/fpd.2017.2344","article-title":"Prevalence and Antibiotic Resistance Profiles of Campylobacter jejuni Isolated from Poultry Meat and Related Samples at Retail Shops in Northern India","volume":"15","author":"Khan","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Udo, E.E., Boswihi, S.S., Mathew, B., Noronha, B., and Verghese, T. (2021). Resurgence of Chloramphenicol Resistance in Methicillin-Resistant Staphylococcus aureus Due to the Acquisition of a Variant Florfenicol Exporter (fexAv)-Mediated Chloramphenicol Resistance in Kuwait Hospitals. Antibiotics, 10.","DOI":"10.3390\/antibiotics10101250"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"802404","DOI":"10.3389\/fmicb.2021.802404","article-title":"Genomic Analysis and Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli in Peru","volume":"12","author":"Quino","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Gharbi, M., B\u00e9jaoui, A., Ben Hamda, C., Ghedira, K., Ghram, A., and Maaroufi, A. (2021). Distribution of virulence and antibiotic re-sistance genes in Campylobacter jejuni and Campylobacter coli isolated from broiler chickens in Tunisia. J. Microbiol. Immunol. Infect.","DOI":"10.1016\/j.jmii.2021.07.001"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1099\/jmm.0.000424","article-title":"Pathogenic potential and genotypic diversity of Campylobacter jejuni: A neglected food-borne pathogen in Brazil","volume":"66","author":"Medeiros","year":"2017","journal-title":"J. Med. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1186\/s13099-016-0132-2","article-title":"Molecular epidemiology, virulence determinants and antimicrobial resistance of Campylobacter spreading in retail chicken meat in Central China","volume":"8","author":"Zhang","year":"2016","journal-title":"Gut Pathog."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1485","DOI":"10.1128\/JCM.01912-07","article-title":"Prevalence of Virulence Genes and Cytolethal Distending Toxin Production in Campylobacter jejuni Isolates from Diarrheal Patients in Bangladesh","volume":"46","author":"Talukder","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1046\/j.1365-2672.2003.01926.x","article-title":"PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates","volume":"94","author":"Bang","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1656","DOI":"10.1016\/S2095-3119(19)62844-3","article-title":"Molecular detection of virulence genes in Campylobacter species isolated from livestock production systems in South Africa","volume":"19","author":"Ngobese","year":"2020","journal-title":"J. Integr. Agric."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1016\/j.foodcont.2019.04.038","article-title":"Prevalence, antimicrobial resistance profiles and virulence-associated genes of thermophilic Campylobacter spp. isolated from ducks in a Chinese slaughterhouse","volume":"104","author":"Han","year":"2019","journal-title":"Food Control"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1089\/fpd.2013.1489","article-title":"Distribution of Nine Virulence-Associated Genes in Campylobacter jejuni and C. coli Isolated from Broiler Feces in Shiraz, Southern Iran","volume":"10","author":"Khoshbakht","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Guirado, P., Paytubi, S., Mir\u00f3, E., Iglesias-Torrens, Y., Navarro, F., Cerd\u00e0-Cu\u00e9llar, M., Attolini, C.S.-O., Balsalobre, C., and Madrid, C. (2020). Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barr\u00e9 Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds. Microorganisms, 8.","DOI":"10.3390\/microorganisms8030325"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1089\/mdr.2015.0055","article-title":"Characterization of Antimicrobial Susceptibility and Its Association with Virulence Genes Related to Adherence, Invasion, and Cytotoxicity in Campylobacter jejuni and Campylobacter coli Isolates from Animals, Meat, and Humans","volume":"22","author":"Lapierre","year":"2016","journal-title":"Microb. Drug Resist."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/S0923-2508(97)85118-2","article-title":"Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals","volume":"147","author":"Linton","year":"1996","journal-title":"Res. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1046\/j.1472-765X.1999.00658.x","article-title":"Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and C. coli","volume":"29","author":"Denis","year":"1999","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_59","unstructured":"(2017, May 16). European Committee on Antimicrobial Susceptibility Testing (EUCAST). Available online: http:\/\/mic.eucast.org."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.mimet.2005.03.013","article-title":"MAMA-PCR assay for the detection of point mutations associated with high-level erythromycin resistance in Campylobacter jejuni and Campylobacter coli strains","volume":"63","author":"Alonso","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.1128\/JCM.37.10.3276-3280.1999","article-title":"Ciprofloxacin Resistance in Campylobacter jejuni Isolates: Detection of gyrA Resistance Mutations by Mismatch Amplification Mutation Assay PCR and DNA Sequence Analysis","volume":"37","author":"Zirnstein","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-6968.2000.tb09253.x","article-title":"Characterization of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli by DNA sequence analysis and MAMA PCR","volume":"190","author":"Zirnstein","year":"2000","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1093\/jac\/dki040","article-title":"Tetracycline resistance of Australian Campylobacter jejuni and Campylobacter coli isolates","volume":"55","author":"Pratt","year":"2005","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1016\/j.fm.2005.06.004","article-title":"Prevalence of the Campylobacter multi-drug efflux pump (CmeABC) in Campylobacter spp. Isolated from freshly processed Turkeys","volume":"23","author":"Olah","year":"2006","journal-title":"Food Microbiol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/j.1365-2672.2012.05354.x","article-title":"Antimicrobial susceptibilities and resistance genes in Campylobacter strains isolated from poultry and pigs in Australia","volume":"113","author":"Obeng","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1099\/jmm.0.05056-0","article-title":"Prevalence of 11 pathogenic genes of Campylobacter jejuni by PCR in strains isolated from humans, poultry meat and broiler and bovine faeces","volume":"52","author":"Datta","year":"2003","journal-title":"J. Med. Microbiol."},{"key":"ref_67","first-page":"55","article-title":"The pathogenesis of Campylobacter jejuni-mediated enteritis","volume":"2","author":"Konkel","year":"2001","journal-title":"Curr. Issues Intest. Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"6535","DOI":"10.1128\/IAI.68.12.6535-6541.2000","article-title":"Campylobacter jejuni Cytolethal Distending Toxin Mediates Release of Interleukin-8 from Intestinal Epithelial Cells","volume":"68","author":"Hickey","year":"2000","journal-title":"Infect. Immun."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1046\/j.1365-2958.2000.02020.x","article-title":"Phase variation of a \u03b2-1,3 galactosyltransferase involved in generation of the ganglioside GM1-like lipo-oligosaccharide of Campylobacter jejuni","volume":"37","author":"Linton","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1128\/jcm.35.3.759-763.1997","article-title":"Specific identification of the enteropathogens Campylobacter jejuni and Campylobacter coli by using a PCR test based on the ceuE gene encoding a putative virulence determinant","volume":"35","author":"Gonzalez","year":"1997","journal-title":"J. Clin. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/7\/830\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:36:16Z","timestamp":1760139376000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/7\/830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,21]]},"references-count":70,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2022,7]]}},"alternative-id":["antibiotics11070830"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11070830","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,21]]}}}