{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:56:23Z","timestamp":1772826983936,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T00:00:00Z","timestamp":1657670400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Institute of General Medical Sciences (NIGMS)","doi-asserted-by":"publisher","award":["P20 GM103429"],"award-info":[{"award-number":["P20 GM103429"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Institute of General Medical Sciences (NIGMS)","doi-asserted-by":"publisher","award":["2117138"],"award-info":[{"award-number":["2117138"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>From a library of compounds, 11 hit antibacterial agents have been identified as potent anti-Gram-positive bacterial agents. These pyrazole derivatives are active against two groups of pathogens, staphylococci and enterococci, with minimum inhibitory concentration (MIC) values as low as 0.78 \u03bcg\/mL. These potent compounds showed bactericidal action, and some were effective at inhibiting and eradicating Staphylococcus aureus and Enterococcus faecalis biofilms. Real-time biofilm inhibition by the potent compounds was studied, by using Bioscreen C. These lead compounds were also very potent against S. aureus persisters as compared to controls, gentamycin and vancomycin. In multiple passage studies, bacteria developed little resistance to these compounds (no more than 2 \u00d7 MIC). The plausible mode of action of the lead compounds is the permeabilization of the cell membrane determined by flow cytometry and protein leakage assays. With the detailed antimicrobial studies, both in planktonic and biofilm contexts, some of these potent compounds have the potential for further antimicrobial drug development.<\/jats:p>","DOI":"10.3390\/antibiotics11070939","type":"journal-article","created":{"date-parts":[[2022,7,13]],"date-time":"2022-07-13T22:06:00Z","timestamp":1657749960000},"page":"939","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Development of 4-[4-(Anilinomethyl)-3-phenyl-pyrazol-1-yl] Benzoic Acid Derivatives as Potent Anti-Staphylococci and Anti-Enterococci Agents"],"prefix":"10.3390","volume":"11","author":[{"given":"Hansa","family":"Raj KC","sequence":"first","affiliation":[{"name":"Department of Chemistry and Physics, The College of Sciences and Mathematics, Arkansas State University, Jonesboro, AR 72401, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4426-1299","authenticated-orcid":false,"given":"David F.","family":"Gilmore","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, The College of Sciences and Mathematics, Arkansas State University, Jonesboro, AR 72401, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0258-3732","authenticated-orcid":false,"given":"Mohammad A.","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Physics, The College of Sciences and Mathematics, Arkansas State University, Jonesboro, AR 72401, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1016\/S0140-6736(21)02724-0","article-title":"Global burden of bacterial antimicrobial resistance in 2019: A systematic analysis","volume":"399","author":"Murray","year":"2022","journal-title":"Lancet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"737635","DOI":"10.3389\/fmicb.2021.737635","article-title":"Non-thermal plasma treatment of ESKAPE pathogens: A review","volume":"12","author":"Scholtz","year":"2021","journal-title":"Front Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/s13756-019-0550-2","article-title":"Control of MSSA and MRSA in the United States: Protocols, policies, risk adjustment and excuses","volume":"8","author":"Kavanagh","year":"2019","journal-title":"Antimicrob. 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