{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:42:26Z","timestamp":1787168546424,"version":"build-2736575974"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T00:00:00Z","timestamp":1658707200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Universidad de Concepci\u00f3n","award":["VRID N\u00b0 218.085.040-1.0IN"],"award-info":[{"award-number":["VRID N\u00b0 218.085.040-1.0IN"]}]},{"name":"Universidad de Concepci\u00f3n","award":["2017 21171278"],"award-info":[{"award-number":["2017 21171278"]}]},{"name":"Universidad de Concepci\u00f3n","award":["FONDECYT 1171805"],"award-info":[{"award-number":["FONDECYT 1171805"]}]},{"name":"National Agency for Research and Development (ANID)\/Scholarship Program\/DOCTORADO NACIONAL","award":["VRID N\u00b0 218.085.040-1.0IN"],"award-info":[{"award-number":["VRID N\u00b0 218.085.040-1.0IN"]}]},{"name":"National Agency for Research and Development (ANID)\/Scholarship Program\/DOCTORADO NACIONAL","award":["2017 21171278"],"award-info":[{"award-number":["2017 21171278"]}]},{"name":"National Agency for Research and Development (ANID)\/Scholarship Program\/DOCTORADO NACIONAL","award":["FONDECYT 1171805"],"award-info":[{"award-number":["FONDECYT 1171805"]}]},{"DOI":"10.13039\/501100002848","name":"National Fund for Scientific and Technological Development (FONDECYT) of Chile","doi-asserted-by":"publisher","award":["VRID N\u00b0 218.085.040-1.0IN"],"award-info":[{"award-number":["VRID N\u00b0 218.085.040-1.0IN"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"National Fund for Scientific and Technological Development (FONDECYT) of Chile","doi-asserted-by":"publisher","award":["2017 21171278"],"award-info":[{"award-number":["2017 21171278"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002848","name":"National Fund for Scientific and Technological Development (FONDECYT) of Chile","doi-asserted-by":"publisher","award":["FONDECYT 1171805"],"award-info":[{"award-number":["FONDECYT 1171805"]}],"id":[{"id":"10.13039\/501100002848","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Macrolides, lincosamides, and type B streptogramins (MLSB) are important therapeutic options to treat methicillin-resistant Staphylococcus aureus (MRSA) infections; however, resistance to these antibiotics has been emerging. In Chile, data on the MLSB resistance phenotypes are scarce in both community-(CA) and hospital-acquired (HA) MRSA isolates. Antimicrobial susceptibility to MLSB was determined for sixty-eight non-repetitive isolates of each HA-(32) and CA-MRSA (36). Detection of SCCmec elements, ermA, ermB, ermC, and msrA genes was performed by PCR. The predominant clones were SCCmec I-ST5 (HA-MRSA) and type IVc-ST8 (CA-MRSA). Most of the HA-MRSA isolates (97%) showed resistance to clindamycin, erythromycin, azithromycin, and clarithromycin. Among CA-MRSA isolates, 28% were resistant to erythromycin, azithromycin, and 25% to clarithromycin. All isolates were susceptible to linezolid, vancomycin, daptomycin and trimethoprim\/sulfamethoxazole, and over 97% to rifampicin. The ermA gene was amplified in 88% of HA-MRSA and 17% of CA-MRSA isolates (p &lt; 0.001). The ermC gene was detected in 6% of HA-SARM and none of CA-SARM isolates, whereas the msrA gene was only amplified in 22% of CA-MRSA (p &lt; 0.005). Our results demonstrate the prevalence of the cMLSB resistance phenotype in all HA-MRSA isolates in Chile, with the ermA being the predominant gene identified among these isolates.<\/jats:p>","DOI":"10.3390\/antibiotics11081000","type":"journal-article","created":{"date-parts":[[2022,7,25]],"date-time":"2022-07-25T21:20:59Z","timestamp":1658784059000},"page":"1000","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Phenotypic and Genotypic Characterization of Macrolide, Lincosamide and Streptogramin B Resistance among Clinical Methicillin-Resistant Staphylococcus aureus Isolates in Chile"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-8368","authenticated-orcid":false,"given":"Mario","family":"Quezada-Aguiluz","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile"},{"name":"Centro Regional de Telemedicina y Telesalud del Biob\u00edo (CRT Biob\u00edo), Concepci\u00f3n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Aguayo-Reyes","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Unidad de Medicina Interna, Hospital Traumatol\u00f3gico de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Infectolog\u00eda, Hospital Regional \u201cDr. Guillermo Grant B.\u201d, Concepci\u00f3n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinthia","family":"Carrasco","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Mej\u00edas","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela","family":"Saavedra","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Mella-Montecinos","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Unidad de Medicina Interna, Hospital Traumatol\u00f3gico de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Infectolog\u00eda, Hospital Regional \u201cDr. Guillermo Grant B.\u201d, Concepci\u00f3n 4030000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6382-7625","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Opazo-Capurro","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-4681","authenticated-orcid":false,"given":"Helia","family":"Bello-Toledo","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7870-1056","authenticated-orcid":false,"given":"Jos\u00e9 M.","family":"Munita","sequence":"additional","affiliation":[{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile"},{"name":"Genomics and Resistant Microbes (GeRM) Group, Facultad de Medicina, Cl\u00ednica Alemana Universidad del Desarrollo, Santiago 7550000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan C.","family":"Hormaz\u00e1bal","sequence":"additional","affiliation":[{"name":"Subdepartamento de Enfermedades Infecciosas, Instituto de Salud P\u00fablica de Chile, Santiago 7780050, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2351-1236","authenticated-orcid":false,"given":"Gerardo","family":"Gonz\u00e1lez-Rocha","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos, Departamento de Microbiolog\u00eda, Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4070386, Chile"},{"name":"Millennium Initiative for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 3580000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,7,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1923","DOI":"10.1007\/s10096-016-2763-0","article-title":"The Remarkably Multifunctional Fibronectin Binding Proteins of Staphylococcus aureus","volume":"35","author":"Foster","year":"2016","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"4251","DOI":"10.3168\/jds.2016-10912","article-title":"Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus in Dairy Sheep and in-Contact Humans: An Intra-Farm Study","volume":"99","author":"Carfora","year":"2016","journal-title":"J. Dairy Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nrmicro2200","article-title":"Waves of Resistance: Staphylococcus aureus in the Antibiotic Era","volume":"7","author":"Chambers","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1007\/s00018-010-0389-4","article-title":"Mobile Genetic Elements of Staphylococcus aureus","volume":"67","author":"Malachowa","year":"2010","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_5","first-page":"81","article-title":"Clinical Practice Guidelines for the Diagnosis and Management of Intravascular Catheter-Related Infection: 2009 Update by the Infectious Diseases Society of America","volume":"10","author":"Mermel","year":"2010","journal-title":"Chin. J. Infect. Chemother."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1342","DOI":"10.1056\/NEJMoa025025","article-title":"Infection with Vancomycin-Resistant Staphylococcus aureus Containing the vanA Resistance Gene","volume":"348","author":"Chang","year":"2003","journal-title":"N. Engl. J. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"445","DOI":"10.4161\/viru.2.5.17724","article-title":"Staphylococcus aureus Biofilms: Properties, Regulation and Roles in Human Disease","volume":"2","author":"Archer","year":"2011","journal-title":"Virulence"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1038\/s41579-018-0147-4","article-title":"Methicillin-Resistant Staphylococcus aureus: An Overview of Basic and Clinical Research","volume":"17","author":"Turner","year":"2019","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/j.bjid.2016.03.003","article-title":"Phenotypic and Molecular Characterization of Resistance to Macrolides, Lincosamides and Type B Streptogramin of Clinical Isolates of Staphylococcus spp. of a University Hospital in Recife, Pernambuco, Brazil","volume":"20","author":"Rabelo","year":"2016","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e00816-17","DOI":"10.1128\/AAC.00816-17","article-title":"A Prospective Cohort Multicenter Study of Molecular Epidemiology and Phylogenomics of Staphylococcus aureus Bacteremia in Nine Latin American Countries","volume":"61","author":"Arias","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1089\/mdr.2018.0259","article-title":"MLSB-Resistant Staphylococcus aureus in Central Greece: Rate of Resistance and Molecular Characterization","volume":"25","author":"Sarrou","year":"2019","journal-title":"Microb. Drug Resist."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1128\/JCM.43.4.1716-1721.2005","article-title":"Testing for Induction of Clindamycin Resistance in Erythromycin-Resistant Isolates of Staphylococcus aureus","volume":"43","author":"Steward","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1128\/AAC.39.3.577","article-title":"Erythromycin Resistance by Ribosome Modification","volume":"39","author":"Weisblum","year":"1995","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Miklas\u00ed, M., and Kostoulias, X. (2021). Mechanisms of Resistance to Macrolide Antibiotics among Staphylococcus aureus. Antibiotics, 10.","DOI":"10.3390\/antibiotics10111406"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"228","DOI":"10.1016\/S0924-8579(03)00218-8","article-title":"Msr(A) and Related Macrolide\/Streptogramin Resistance Determinants: Incomplete Transporters?","volume":"22","author":"Reynolds","year":"2003","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"315","DOI":"10.1093\/jac\/48.2.315","article-title":"Clindamycin Treatment of Staphylococcus aureus Expressing Inducible Clindamycin Resistance","volume":"48","author":"Drinkovic","year":"2001","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.plasmid.2018.05.001","article-title":"Mobile Macrolide Resistance Genes in Staphylococci","volume":"99","author":"Wang","year":"2018","journal-title":"Plasmid"},{"key":"ref_18","unstructured":"Ota\u00edza, F., Orsini, M., Pohlenz, M., and Tarride, T. (2022, March 13). Informe de Vigilancia de Infecciones Asociadas a la Atenci\u00f3n en Salud. Available online: https:\/\/www.minsal.cl\/wpcontent\/uploads\/2015\/09\/informe-vigilancia-2017.pdf."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1007\/s10096-013-1907-8","article-title":"Molecular Epidemiology of Hospital-Onset Methicillin-Resistant Staphylococcus aureus Infections in Southern Chile","volume":"32","author":"Medina","year":"2013","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_20","unstructured":"Instituto de Salud P\u00fablica de Chile (2017). Vigilancia de Staphylococcus aureus Meticilina Resistente Adquirido en la Comunidad, Instituto de Salud P\u00fablica de Chile. Available online: https:\/\/www.ispch.cl\/sites\/default\/files\/BoletinStahylococcusResistente-20062018A%20(1).pdf."},{"key":"ref_21","first-page":"202","article-title":"Macrolide-Lincosamide-Streptogramin B Resistance Phenotypes and Their Associated Genotypes in Staphylococcus aureus Isolates from a Tertiary Level Public Hospital of Uruguay","volume":"52","author":"Pardo","year":"2020","journal-title":"Rev. Argent. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.ijid.2006.09.005","article-title":"Characterization of Macrolide Resistance in Gram-Positive Cocci from Colombian Hospitals: A Countrywide Surveillance","volume":"11","author":"Reyes","year":"2007","journal-title":"Int. J. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1861","DOI":"10.1086\/648426","article-title":"Dissemination of Methicillin-Resistant Staphylococcus aureus (MRSA), USA300 Sequence Type 8 Lineage in Latin-America","volume":"49","author":"Reyes","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1086","DOI":"10.1016\/j.ijmm.2014.08.002","article-title":"New Patterns of Methicillin-Resistant Staphylococcus aureus (MRSA) Clones, Community-Associated MRSA Genotypes Behave like Healthcare-Associated MRSA Genotypes within Hospitals, Argentina","volume":"304","author":"Egea","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1874","DOI":"10.1093\/infdis\/jiv320","article-title":"Parallel Epidemics of Community-Associated Methicillin-Resistant Staphylococcus aureus USA300 Infection in North and South America","volume":"212","author":"Planet","year":"2015","journal-title":"J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"588","DOI":"10.4067\/S0716-10182015000600017","article-title":"Aislamiento de Staphylococcus aureus Hetero-Resistente a Vancomicina En Hospital Cl\u00ednico Regional de Concepci\u00f3n, Chile","volume":"32","author":"Vega","year":"2015","journal-title":"Revista chilena de infectolog\u00eda"},{"key":"ref_27","unstructured":"Clinical and Laboratory Standards Institute (2003). Performance Standards for Antimicrobial Disk Susceptibility Tests: Approved Standard, Clinical and Laboratory Standards Institute."},{"key":"ref_28","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2018). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard\u2014Ninth Edition, CLSI. [11th ed.]. CLSI Document M07-A9."},{"key":"ref_29","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2020). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement, CLSI. [30th ed.]. CLSI Document M100."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"218","DOI":"10.1016\/j.mcp.2012.04.002","article-title":"Enhanced Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome mec Types I to V in Methicillin-Resistant Staphylococcus aureus","volume":"26","author":"Zhang","year":"2012","journal-title":"Mol. Cell. Probes"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/AAC.00165-06","article-title":"Combination of Multiplex PCRs for Staphylococcal Cassette Chromosome mec Type Assignment: Rapid Identification System for mec, ccr, and Major Differences in Junkyard Regions","volume":"51","author":"Kondo","year":"2007","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1128","DOI":"10.1086\/313461","article-title":"Involvement of Panton-Valentine Leukocidin-Producing Staphylococcus aureus in Primary Skin Infections and Pneumonia","volume":"29","author":"Lina","year":"1999","journal-title":"Clin. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"328","DOI":"10.3389\/fcimb.2019.00328","article-title":"Genetic Features of Antarctic Acinetobacter radioresistens Strain A154 Harboring Multiple Antibiotic-Resistance Genes","volume":"9","author":"Higgins","year":"2019","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_34","first-page":"886","article-title":"Detection of Aminoglycoside and Macrolide Resistance Mechanisms in Methicillin-Resistant Staphylococcus aureus","volume":"120","author":"Dorneanu","year":"2016","journal-title":"Rev. Med. Chir. Soc. Med. Nat. Iasi"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3145","DOI":"10.1038\/s41598-020-60035-0","article-title":"Antibiotic Resistance in Bacterial Isolates from Freshwater Samples in Fildes Peninsula, King George Island, Antarctica","volume":"10","author":"Jara","year":"2020","journal-title":"Sci. Rep."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/8\/1000\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T23:56:10Z","timestamp":1760140570000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/8\/1000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,25]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2022,8]]}},"alternative-id":["antibiotics11081000"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11081000","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,25]]}}}