{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:27:08Z","timestamp":1793726828007,"version":"4.6.1"},"reference-count":37,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T00:00:00Z","timestamp":1661040000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: There is great interest in the search for new alternatives to antimicrobial drugs, and the use of synbiotics is a promising approach to this problem. This study evaluated the growth inhibition and antibiofilm activity of the short-chain fatty acids produced by Lacticaseibacillus rhamnosus and Pediococcus acidilactici in combination with inulin-type fructans against Candida albicans. Methods: The growth inhibition of Candida was evaluated using microdilution analysis in 96-well microtiter plates; different concentrations of cell-free supernatants of Lacticaseibacillus rhamnosus and Pediococcus acidilactici were exposed to Candida albicans. The antibiofilm assessment was carried out using the crystal violet staining assay. The short-chain fatty acids were analyzed by gas chromatography. Results: The clinically isolated Candida albicans interacted with supernatants from Lacticaseibacillus rhamnosus and Pediococcus acidilactici and showed significant growth inhibition and antibiofilm formation versus the controls. Lactate and acetic acid were elevated in the supernatants. The results suggest that the supernatants obtained from the synbiotic combinations of Lacticaseibacillus rhamnosus and Pediococcus acidilactici with inulin-type fructans can inhibit the growth and biofilm formation against a clinically isolated Candida albicans strain. Conclusions: These results suggest that synbiotic formulations could be a promising alternative to antifungal drugs in candidiasis therapy.<\/jats:p>","DOI":"10.3390\/antibiotics11081135","type":"journal-article","created":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T21:05:51Z","timestamp":1661115951000},"page":"1135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Anticandidal and Antibiofilm Effect of Synbiotics including Probiotics and Inulin-Type Fructans"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7226-3315","authenticated-orcid":false,"given":"Ricardo","family":"Garc\u00eda-Gamboa","sequence":"first","affiliation":[{"name":"Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0400-6680","authenticated-orcid":false,"given":"Miguel","family":"Dom\u00ednguez-Simi","sequence":"additional","affiliation":[{"name":"Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8236-4400","authenticated-orcid":false,"given":"Misael S.","family":"Gradilla-Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Tecnol\u00f3gico de Monterrey, Escuela de Ingenier\u00eda y Ciencias, Av. General Ram\u00f3n Corona 2514, Nuevo Mexico, Zapopan 45138, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6977-9778","authenticated-orcid":false,"given":"Jorge","family":"Bravo","sequence":"additional","affiliation":[{"name":"Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2867-1119","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Moya","sequence":"additional","affiliation":[{"name":"Foundation for the Promotion of Health and Biomedical Research in the Valencian Community Public Health (FISABIO), 46020 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca","family":"Ruiz-\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisela","family":"Gonz\u00e1lez-Avila","sequence":"additional","affiliation":[{"name":"Centre for Research and Assistance in Technology and Design of the State of Jalisco A.C., Normalistas 800, Guadalajara 44270, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,8,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.cofs.2020.03.009","article-title":"Probiotic: Conceptualization from a New Approach","volume":"32","author":"Zendeboodi","year":"2020","journal-title":"Curr. 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