{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T06:57:17Z","timestamp":1762066637914,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T00:00:00Z","timestamp":1662508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"The National Fund for Scientific and Technological Development","award":["1130838","11190602","NCN17_081"],"award-info":[{"award-number":["1130838","11190602","NCN17_081"]}]},{"name":"ANID Millennium Science Initiative\/Millennium Initiative for Collaborative Research on Bacterial Resistance","award":["1130838","11190602","NCN17_081"],"award-info":[{"award-number":["1130838","11190602","NCN17_081"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Carbapenem-resistant Enterobacterales (CRE) is a critical public health problem in South America, where the prevalence of NDM metallo-betalactamases has increased substantially in recent years. In this study, we used whole genome sequencing to characterize a multidrug-resistant (MDR) Klebsiella pneumoniae (UCO-361 strain) clinical isolate from a teaching hospital in Chile. Using long-read (Nanopore) and short-read (Illumina) sequence data, we identified a novel un-typeable megaplasmid (314,976 kb, pNDM-1_UCO-361) carrying the blaNDM-1 carbapenem resistance gene within a Tn3000 transposon. Strikingly, conjugal transfer of pNDM-1_UCO-361 plasmid only occurs at low temperatures with a high frequency of 4.3 \u00d7 10\u22126 transconjugants\/receptors at 27 \u00b0C. UCO-361 belonged to the ST1588 clone, previously identified in Latin America, and harbored aminoglycoside, extended-spectrum \u03b2-lactamases (ESBLs), carbapenem, and quinolone-resistance determinants. These findings suggest that blaNDM-1-bearing megaplasmids can be adapted to carriage by some K. pneumoniae lineages, whereas its conjugation at low temperatures could contribute to rapid dissemination at the human\u2013environmental interface.<\/jats:p>","DOI":"10.3390\/antibiotics11091207","type":"journal-article","created":{"date-parts":[[2022,9,7]],"date-time":"2022-09-07T20:52:03Z","timestamp":1662583923000},"page":"1207","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel Megaplasmid Driving NDM-1-Mediated Carbapenem Resistance in Klebsiella pneumoniae ST1588 in South America"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9644-8368","authenticated-orcid":false,"given":"Mario","family":"Quezada-Aguiluz","sequence":"first","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 8320000, Chile"},{"name":"Centro Regional de Telemedicina y Telesalud del Biob\u00edo (CRT Biob\u00edo), Concepci\u00f3n 4030000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6382-7625","authenticated-orcid":false,"given":"Andr\u00e9s","family":"Opazo-Capurro","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0161-5800","authenticated-orcid":false,"given":"Nilton","family":"Lincopan","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Department of Microbiology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda","family":"Esposito","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna","family":"Fuga","sequence":"additional","affiliation":[{"name":"Department of Clinical Analysis, School of Pharmacy, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"},{"name":"Department of Microbiology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Mella-Montecino","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Infectolog\u00eda, Hospital Regional \u201cDr. Guillermo Grant Benavente\u201d, Concepci\u00f3n 4030000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gisela","family":"Riedel","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Unidad de Infectolog\u00eda, Hospital Regional \u201cDr. Guillermo Grant Benavente\u201d, Concepci\u00f3n 4030000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celia A.","family":"Lima","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9277-4681","authenticated-orcid":false,"given":"Helia","family":"Bello-Toledo","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela","family":"Cifuentes","sequence":"additional","affiliation":[{"name":"Servicio de Laboratorio Cl\u00ednico, Hospital Cl\u00ednico Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco","family":"Silva-Ojeda","sequence":"additional","affiliation":[{"name":"Servicio de Laboratorio Cl\u00ednico, Hospital Cl\u00ednico Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Boris","family":"Barrera","sequence":"additional","affiliation":[{"name":"Servicio de Laboratorio Cl\u00ednico, Hospital Cl\u00ednico Universidad de Chile, Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Hormaz\u00e1bal","sequence":"additional","affiliation":[{"name":"Subdepartamento de Enfermedades Infecciosas, Instituto de Salud P\u00fablica de Chile (ISP), Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2351-1236","authenticated-orcid":false,"given":"Gerardo","family":"Gonz\u00e1lez-Rocha","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Agentes Antibacterianos (LIAA-UdeC), Facultad de Ciencias Biol\u00f3gicas, Universidad de Concepci\u00f3n, Concepci\u00f3n 4030000, Chile"},{"name":"Millennium Nucleus for Collaborative Research on Bacterial Resistance (MICROB-R), Santiago 8320000, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S20","DOI":"10.1016\/j.amjmed.2006.03.013","article-title":"Resistance in Gram-Negative Bacteria: Enterobacteriaceae","volume":"119","author":"Paterson","year":"2006","journal-title":"Am. 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