{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T19:07:58Z","timestamp":1793128078568,"version":"4.5.0"},"reference-count":55,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development, AMED","doi-asserted-by":"publisher","award":["JP21fk0108103"],"award-info":[{"award-number":["JP21fk0108103"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development, AMED","doi-asserted-by":"publisher","award":["JP22fk0108131"],"award-info":[{"award-number":["JP22fk0108131"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development, AMED","doi-asserted-by":"publisher","award":["H30 Shinkogyosei-Ippan-002"],"award-info":[{"award-number":["H30 Shinkogyosei-Ippan-002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development, AMED","doi-asserted-by":"publisher","award":["R4 Shinkogyosei-Ippan-002"],"award-info":[{"award-number":["R4 Shinkogyosei-Ippan-002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Health, Labour and Welfare, Japan","award":["JP21fk0108103"],"award-info":[{"award-number":["JP21fk0108103"]}]},{"name":"Ministry of Health, Labour and Welfare, Japan","award":["JP22fk0108131"],"award-info":[{"award-number":["JP22fk0108131"]}]},{"name":"Ministry of Health, Labour and Welfare, Japan","award":["H30 Shinkogyosei-Ippan-002"],"award-info":[{"award-number":["H30 Shinkogyosei-Ippan-002"]}]},{"name":"Ministry of Health, Labour and Welfare, Japan","award":["R4 Shinkogyosei-Ippan-002"],"award-info":[{"award-number":["R4 Shinkogyosei-Ippan-002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>To characterize environmental antimicrobial resistance (AMR) in urban areas, extended-spectrum \u03b2-lactamase- (ESBL)\/carbapenemase-producing bacteria (EPB\/CPB, respectively) from urban wastewater treatment plant effluents in Tokyo were isolated on CHROMagar ESBL plate. Complete genome sequence analysis, including plasmids, indicated that 126 CTX-M-positive isolates (31%) were identified among the 404 obtained isolates. The CTX-M-9 group was predominant (n = 65, 52%), followed by the CTX-M-1 group (n = 44, 35%). Comparative genome analysis revealed that CTX-M-27-positive E. coli O16:H5-ST131-fimH41 exhibited a stable genome structure and clonal-global dissemination. Plasmidome network analysis revealed that 304 complete plasmid sequences among 85 isolates were grouped into 14 incompatibility (Inc) network communities (Co1 to Co14). Co10 consisted of primarily IncFIA\/IncFIB plasmids harboring blaCTX-M in E. coli, whereas Co12 consisted primarily of IncFIA(HI1)\/Inc FIB(K) plasmids harboring blaCTX-M, blaKPC, and blaGES in Klebsiella spp. Co11 was markedly located around Co10 and Co12. Co11 exhibited blaCTX-M, blaKPC, and blaNDM, and was mainly detected in E. coli and Klebsiella spp. from human and animal sources, suggesting a mutual role of Co11 in horizontal gene transfer between E. coli and Klebsiella spp. This comprehensive resistome analysis uncovers the mode of relational transfer among bacterial species, highlighting the potential source of AMR burden on public health in urban communities.<\/jats:p>","DOI":"10.3390\/antibiotics11101283","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T21:22:23Z","timestamp":1663795343000},"page":"1283","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Comprehensive Genome and Plasmidome Analysis of Antimicrobial Resistant Bacteria in Wastewater Treatment Plant Effluent of Tokyo"],"prefix":"10.3390","volume":"11","author":[{"given":"Tsuyoshi","family":"Sekizuka","sequence":"first","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rina","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Hashino","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Yatsu","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0487-4405","authenticated-orcid":false,"given":"Makoto","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Pathogen Genomics Center, National Institute of Infectious Diseases, Shinjyuku-ku, Tokyo 162-8640, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,9,21]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2016). 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