{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T23:50:11Z","timestamp":1762300211632,"version":"build-2065373602"},"reference-count":28,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Key R&amp;D Program of China","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Science and Technology Program","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}]},{"DOI":"10.13039\/501100012151","name":"Sanming Project of Medicine in Shenzhen","doi-asserted-by":"publisher","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}],"id":[{"id":"10.13039\/501100012151","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Shenzhen Key Medical Discipline Construction Fund","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}]},{"name":"Shenzhen Science and Technology Planning Project","award":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"],"award-info":[{"award-number":["2019YFC1605104","22193064","JCYJ20210324124201004","SZSM202011008","SZXK066","JCYJ20170413101841798"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Phorate is a systemic, broad-spectrum organophosphorus insecticide. Although it is commonly used worldwide, phorate, like other pesticides, not only causes environmental pollution but also poses serious threats to human and animal health. Herein, we measured the blood glucose concentrations of high-fat-diet-fed mice exposed to various concentrations of phorate (0, 0.005, 0.05, or 0.5 mg\/kg); we also assessed the blood glucose concentrations of high-fat-diet-fed mice exposed to phorate; we also assessed the distribution characteristics of the resistance genes in the intestinal microbiota of these mice. We found that 0.005 and 0.5 mg\/kg of phorate induced obvious hyperglycaemia in the high-fat-diet-fed mice. Exposure to phorate markedly reduced the abundance of Akkermansia muciniphila in the mouse intestine. The resistance genes vanRG, tetW\/N\/W, acrD, and evgS were significantly upregulated in the test group compared with the control group. Efflux pumping was the primary mechanism of drug resistance in the Firmicutes, Proteobacteria, Bacteroidetes, Verrucomicrobia, Synergistetes, Spirochaetes, and Actinobacteria found in the mouse intestine. Our findings indicate that changes in the abundance of the intestinal microbiota are closely related to the presence of antibiotic-resistant bacteria in the intestinal tract and the metabolic health of the host.<\/jats:p>","DOI":"10.3390\/antibiotics11111584","type":"journal-article","created":{"date-parts":[[2022,11,10]],"date-time":"2022-11-10T02:01:45Z","timestamp":1668045705000},"page":"1584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Phorate on the Development of Hyperglycaemia in Mouse and Resistance Genes in Intestinal Microbiota"],"prefix":"10.3390","volume":"11","author":[{"given":"Tingting","family":"Cao","sequence":"first","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yajie","family":"Guo","sequence":"additional","affiliation":[{"name":"The Eighth Affiliated Hospital, Sun Yat-Sen University, Shenzhen 518033, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suli","family":"Huang","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changfeng","family":"Peng","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0866-4584","authenticated-orcid":false,"given":"Shaolin","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Lu","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fan","family":"Xiao","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoyi","family":"Liang","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sijia","family":"Zheng","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianzhong","family":"Shen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, China Agricultural University, Beijing 100091, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6430-1302","authenticated-orcid":false,"given":"Yongning","family":"Wu","sequence":"additional","affiliation":[{"name":"Food Safety Research Unit (2019RU014), Chinese Academy of Medical Science, NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ziquan","family":"Lv","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6252-0904","authenticated-orcid":false,"given":"Yuebin","family":"Ke","sequence":"additional","affiliation":[{"name":"Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China"},{"name":"School of Public Health, Southern Medical University, Guangzhou 510515, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1039\/C4MB00044G","article-title":"Metabonomic analysis of the joint toxic action of long-term low-level exposure to a mixture of four organophosphate pesticides in rat plasma","volume":"10","author":"Du","year":"2014","journal-title":"Mol. 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