{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:08:51Z","timestamp":1789502931240,"version":"build-2803163510"},"reference-count":63,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T00:00:00Z","timestamp":1668470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Spanish Ministry of Science, Innovation and Universities","award":["RTI2018-101991-B-I00"],"award-info":[{"award-number":["RTI2018-101991-B-I00"]}]},{"name":"Spanish Ministry of Science, Innovation and Universities","award":["PTQ2018-009961"],"award-info":[{"award-number":["PTQ2018-009961"]}]},{"name":"Spanish Ministry of Science, Innovation and Universities","award":["2017DI037"],"award-info":[{"award-number":["2017DI037"]}]},{"name":"European Social Fund and the Spanish Ministry of Science and Innovation","award":["RTI2018-101991-B-I00"],"award-info":[{"award-number":["RTI2018-101991-B-I00"]}]},{"name":"European Social Fund and the Spanish Ministry of Science and Innovation","award":["PTQ2018-009961"],"award-info":[{"award-number":["PTQ2018-009961"]}]},{"name":"European Social Fund and the Spanish Ministry of Science and Innovation","award":["2017DI037"],"award-info":[{"award-number":["2017DI037"]}]},{"name":"AGAUR, Generalitat de Catalunya, Spain","award":["RTI2018-101991-B-I00"],"award-info":[{"award-number":["RTI2018-101991-B-I00"]}]},{"name":"AGAUR, Generalitat de Catalunya, Spain","award":["PTQ2018-009961"],"award-info":[{"award-number":["PTQ2018-009961"]}]},{"name":"AGAUR, Generalitat de Catalunya, Spain","award":["2017DI037"],"award-info":[{"award-number":["2017DI037"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Staphylococcus pseudintermedius, a common commensal canine bacterium, is the main cause of skin infections in dogs and is a potential zoonotic pathogen. The emergence of methicillin-resistant S. pseudintermedius (MRSP) has compromised the treatment of infections caused by these bacteria. In this study, we compared the phenotypic results obtained by minimum inhibitory concentration (MICs) for 67 S. pseudintermedius isolates from the skin of nine healthy dogs versus the genotypic data obtained with Nanopore sequencing. A total of 17 antibiotic resistance genes (ARGs) were detected among the isolates. A good correlation between phenotype and genotype was observed for some antimicrobial classes, such as ciprofloxacin (fluoroquinolone), macrolides, or tetracycline. However, for oxacillin (beta-lactam) or aminoglycosides the correlation was low. Two antibiotic resistance genes were located on plasmids integrated in the chromosome, and a third one was in a circular plasmid. To our knowledge, this is the first study assessing the correlation between phenotype and genotype regarding antimicrobial resistance of S. pseudintermedius from healthy dogs using Nanopore sequencing technology.<\/jats:p>","DOI":"10.3390\/antibiotics11111625","type":"journal-article","created":{"date-parts":[[2022,11,16]],"date-time":"2022-11-16T02:27:34Z","timestamp":1668565654000},"page":"1625","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Concordance between Antimicrobial Resistance Phenotype and Genotype of Staphylococcus pseudintermedius from Healthy Dogs"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0709-5236","authenticated-orcid":false,"given":"Joaquim","family":"Vi\u00f1es","sequence":"first","affiliation":[{"name":"Vetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"},{"name":"Molecular Genetics Veterinary Service (SVGM), Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norma","family":"F\u00e0bregas","sequence":"additional","affiliation":[{"name":"Vetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9574-5755","authenticated-orcid":false,"given":"Anna","family":"Cusc\u00f3","sequence":"additional","affiliation":[{"name":"Vetgenomics, Edifici EUREKA, PRUAB, Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"},{"name":"Institute of Science and Technology for Brain-Inspired Intelligence, Fudan University, Shanghai 200433, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roc\u00edo","family":"Fonticoba","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-3835","authenticated-orcid":false,"given":"Olga","family":"Francino","sequence":"additional","affiliation":[{"name":"Molecular Genetics Veterinary Service (SVGM), Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1537-0074","authenticated-orcid":false,"given":"Llu\u00eds","family":"Ferrer","sequence":"additional","affiliation":[{"name":"Department of Animal Medicine and Surgery, Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2935-928X","authenticated-orcid":false,"given":"Lourdes","family":"Migura-Garcia","sequence":"additional","affiliation":[{"name":"Unitat mixta d\u2019Investigaci\u00f3 IRTA-UAB en Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"},{"name":"Institut de Recerca i Tecnologia Agroaliment\u00e0ries (IRTA), Programa de Sanitat Animal, Centre de Recerca en Sanitat Animal (CReSA), Campus de la Universitat Aut\u00f2noma de Barcelona (UAB), Bellaterra, 08193 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"253-e52","DOI":"10.1111\/j.1365-3164.2012.01046.x","article-title":"Staphylococcus pseudintermedius in the dog: Taxonomy, diagnostics, ecology, epidemiology and pathogenicity","volume":"23","author":"Bannoehr","year":"2012","journal-title":"Vet. 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