{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T13:01:11Z","timestamp":1789563671628,"version":"build-2803163510"},"reference-count":33,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T00:00:00Z","timestamp":1668988800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Basic Science Research Program","award":["NRF-2016R1D1A3A03918533"],"award-info":[{"award-number":["NRF-2016R1D1A3A03918533"]}]},{"name":"Basic Science Research Program","award":["NIE-A-2022-07"],"award-info":[{"award-number":["NIE-A-2022-07"]}]},{"name":"Basic Science Research Program","award":["NIE-A-2022-11"],"award-info":[{"award-number":["NIE-A-2022-11"]}]},{"name":"National Institute of Ecology (NIE)","award":["NRF-2016R1D1A3A03918533"],"award-info":[{"award-number":["NRF-2016R1D1A3A03918533"]}]},{"name":"National Institute of Ecology (NIE)","award":["NIE-A-2022-07"],"award-info":[{"award-number":["NIE-A-2022-07"]}]},{"name":"National Institute of Ecology (NIE)","award":["NIE-A-2022-11"],"award-info":[{"award-number":["NIE-A-2022-11"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Although considerable scientific research data is available for sepsis and cytokine storm syndrome, there is a need to develop new treatments or drugs for sepsis management. Antimicrobial peptides (AMPs) possess anti-bacterial and anti-inflammatory activity, neutralizing toxins such as lipopolysaccharides (LPS, endotoxin). Most AMPs have been designed as a substitute for conventional antibiotics, which kill drug-resistant pathogens. The present study aimed to determine the anti-inflammatory potential of 10 designed XIW (X: lysine, arginine, or glutamic acid) \u03b1-helical peptides in macrophages and a mouse model in the presence of LPS. Among them, WIKE-14, a peptide with a helix-to-helix structure, having the 12th amino acid substituted with glutamic acid, suppressed pro-inflammatory cytokines in RAW 264.7 macrophages. This reaction was mediated by the inhibition of the binding between LPS and macrophages. In addition, the WIKE-14 peptide exhibited a potent anti-inflammatory activity in mice ears and lungs inflamed using LPS. Thus, our results may provide useful insights for the development of anti-sepsis agents via the sequence and structure information of the WIKE-14 peptide.<\/jats:p>","DOI":"10.3390\/antibiotics11111675","type":"journal-article","created":{"date-parts":[[2022,11,22]],"date-time":"2022-11-22T03:02:16Z","timestamp":1669086136000},"page":"1675","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Potent Anti-Inflammatory Effects of a Helix-to-Helix Peptide against Pseudomonas aeruginosa Endotoxin-Mediated Sepsis"],"prefix":"10.3390","volume":"11","author":[{"given":"Hyosuk","family":"Son","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"},{"name":"Department of Exhibition and Education, National Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9051-0183","authenticated-orcid":false,"given":"Seong-Cheol","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Young-Min","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jong-Kook","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyoung","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taeuk","family":"Guk","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A-Mi","family":"Yoon","sequence":"additional","affiliation":[{"name":"LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea"},{"name":"Division of Life Sciences, Jeonbuk National University, Jeonju 54896, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hye Song","family":"Lim","sequence":"additional","affiliation":[{"name":"LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mi-Kyeong","family":"Jang","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering, Sunchon National University, Suncheon 57922, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9596-3330","authenticated-orcid":false,"given":"Jung Ro","family":"Lee","sequence":"additional","affiliation":[{"name":"LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,11,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1017\/S1462399404007409","article-title":"Sepsis: Molecular mechanisms underlying lipopolysaccharide recognition","volume":"6","author":"Triantafilou","year":"2004","journal-title":"Expert Rev. 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