{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T10:57:12Z","timestamp":1778669832983,"version":"3.51.4"},"reference-count":92,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T00:00:00Z","timestamp":1671148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008431","name":"Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["LE018P20"],"award-info":[{"award-number":["LE018P20"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008431","name":"Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["RTI2018-098267-R-C33"],"award-info":[{"award-number":["RTI2018-098267-R-C33"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008431","name":"Junta de Castilla y Le\u00f3n","doi-asserted-by":"publisher","award":["18BB282"],"award-info":[{"award-number":["18BB282"]}],"id":[{"id":"10.13039\/501100008431","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["LE018P20"],"award-info":[{"award-number":["LE018P20"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["RTI2018-098267-R-C33"],"award-info":[{"award-number":["RTI2018-098267-R-C33"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014440","name":"Ministerio de Ciencia, Innovaci\u00f3n y Universidades","doi-asserted-by":"publisher","award":["18BB282"],"award-info":[{"award-number":["18BB282"]}],"id":[{"id":"10.13039\/100014440","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Universidad de Le\u00f3n","award":["LE018P20"],"award-info":[{"award-number":["LE018P20"]}]},{"name":"Universidad de Le\u00f3n","award":["RTI2018-098267-R-C33"],"award-info":[{"award-number":["RTI2018-098267-R-C33"]}]},{"name":"Universidad de Le\u00f3n","award":["18BB282"],"award-info":[{"award-number":["18BB282"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Twenty samples of minced chicken meat procured from butcher\u2019s shops in Le\u00f3n (Spain; 10 samples) and Vila Real (Portugal; 10 samples) were analyzed. Microbial concentrations (log10 cfu\/g) of 7.53 \u00b1 1.02 (viable aerobic microbiota), 7.13 \u00b1 1.07 (psychrotrophic microorganisms), and 4.23 \u00b1 0.88 (enterobacteria) were found. The detection method described in the UNE-EN ISO 11290-1 standard (based on isolation from the chromogenic medium OCLA) with confirmation by the polymerase chain reaction (PCR; lmo1030) (OCLA\u2013PCR), revealed Listeria monocytogenes in 14 samples (70.0% of the total), nine of Spanish origin and five of Portuguese (p &gt; 0.05). The levels of viable and inactivated L. monocytogenes in the samples were determined with a q-PCR using propidium monoazide (PMAxx) as a viability marker. Seven samples tested positive both with the OCLA\u2013PCR and with the q-PCR, with estimated concentrations of viable cells varying between 2.15 log10 cfu\/g (detection limit) and 2.94 log10 cfu\/g. Three samples tested negative both with the OCLA\u2013PCR and with the q-PCR. Seven samples were positive with the OCLA\u2013PCR, but negative with the q-PCR, and three samples tested negative with the OCLA\u2013PCR and positive with the q-PCR. The percentage of viable cells relative to the total ranged between 2.4% and 86.0%. Seventy isolates of L. monocytogenes (five from each positive sample) were classified in PCR serogroups with a multiplex PCR assay. L. monocytogenes isolates belonged to serogroups IIa (52 isolates; 74.3%), IIc (7; 10.0%), IVa (2; 2.9%), and IVb (9; 12.9%). The susceptibility of the 70 isolates to 15 antibiotics of clinical interest was tested. The strains presented resistance to between three and eight antibiotics. The average number of resistances was greater (p &lt; 0.001) among strains isolated from Spanish samples (6.20 \u00b1 1.08), than in those from Portugal (5.00 \u00b1 1.08). In both groups of strains, a prevalence of resistance higher than 95% was observed for oxacillin, cefoxitin, cefotaxime, and cefepime. The need to handle minced chicken meat correctly, taking care to cook it sufficiently and to avoid cross-contamination, so as to reduce the danger of listeriosis, is emphasized. A combination of culture-dependent and culture-independent methods offers complementary routes for the detection in food of the cells of L. monocytogenes in various different physiological states.<\/jats:p>","DOI":"10.3390\/antibiotics11121828","type":"journal-article","created":{"date-parts":[[2022,12,16]],"date-time":"2022-12-16T02:16:41Z","timestamp":1671157001000},"page":"1828","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantification of Total and Viable Cells and Determination of Serogroups and Antibiotic Resistance Patterns of Listeria monocytogenes in Chicken Meat from the North-Western Iberian Peninsula"],"prefix":"10.3390","volume":"11","author":[{"given":"Cristina","family":"Rodr\u00edguez-Melc\u00f3n","sequence":"first","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Esteves","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences, School of Agrarian and Veterinary Sciences, University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"},{"name":"Veterinary and Animal Research Centre (CECAV), University of Tr\u00e1s-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah","family":"Panera-Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9584-0755","authenticated-orcid":false,"given":"Rosa","family":"Capita","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-0912","authenticated-orcid":false,"given":"Carlos","family":"Alonso-Calleja","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene and Technology, Veterinary Faculty, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"},{"name":"Institute of Food Science and Technology, University of Le\u00f3n, E-24071 Le\u00f3n, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"ref_1","unstructured":"FAOSTAT (2021, September 22). Consumo Carne de Pollo. Available online: http:\/\/www.fao.org\/faostat\/es\/#data\/QL."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.meatsci.2012.09.018","article-title":"Meat nutritional composition and nutritive role in the human diet","volume":"93","year":"2013","journal-title":"Meat Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Capita, R., Casta\u00f1o-Arriba, A., Rodr\u00edguez-Melc\u00f3n, C., Igrejas, G., Poeta, P., and Alonso-Calleja, C. (2020). Diversity, antibiotic resistance, and biofilm-forming ability of enterobacteria isolated from red meat and poultry preparations. Microorganisms, 8.","DOI":"10.3390\/microorganisms8081226"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4046","DOI":"10.3382\/ps\/pex200","article-title":"Microbial loads and antibiotic resistance patterns of Staphylococcus aureus in different types of raw poultry-based meat preparations","volume":"96","author":"Capita","year":"2017","journal-title":"Poult. Sci."},{"key":"ref_5","first-page":"55","article-title":"Microbial quality of retail meat products available in Chennai city","volume":"2","author":"Selvan","year":"2007","journal-title":"Am. J. Food Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1016\/j.ijfoodmicro.2006.10.048","article-title":"Effect of various chemical decontamination treatments on natural microflora and sensory characteristics of poultry","volume":"115","author":"Prieto","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1016\/j.meatsci.2017.01.007","article-title":"Lactic acid concentrations that reduce microbial load yet minimally impact colour and sensory characteristics of beef","volume":"129","author":"Capita","year":"2017","journal-title":"Meat Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0309-1740(01)00225-X","article-title":"Microbiological quality of retail chicken by-products in Spain","volume":"62","author":"Capita","year":"2002","journal-title":"Meat Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1016\/j.fm.2018.06.016","article-title":"Structure and viability of 24- and 72-h-old biofilms formed by four pathogenic bacteria on polystyrene and glass contact surfaces","volume":"76","author":"Carballo","year":"2018","journal-title":"Food Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1016\/j.fm.2019.03.020","article-title":"Susceptibility of Listeria monocytogenes planktonic cultures and biofilms to sodium hypochlorite and benzalkonium chloride","volume":"82","author":"Capita","year":"2019","journal-title":"Food Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S1473-3099(14)70870-9","article-title":"The global burden of listeriosis: A systematic review and meta-analysis","volume":"14","author":"Devleesschauwer","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"EFSA, and ECDC (2021). The European Union One Health 2019 Zoonoses Report. EFSA J., 19, e06406.","DOI":"10.2903\/j.efsa.2021.6406"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"792162","DOI":"10.3389\/fmicb.2021.792162","article-title":"Different shades of Listeria monocytogenes: Strain, serotype, and lineage-based variability in virulence and stress tolerance profiles","volume":"4","author":"Muchaamba","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"54","DOI":"10.4315\/JFP-21-172","article-title":"Diversity and antibiotic resistance profiles of Listeria monocytogenes serogroups in different food products from the Transylvania Region of Central Romania","volume":"85","author":"Herwman","year":"2022","journal-title":"J. Food Prot."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Alonso-Calleja, C., G\u00f3mez-Fern\u00e1ndez, S., Carballo, J., and Capita, R. (2019). Prevalence, molecular typing, and determination of the biofilm-forming ability of Listeria monocytogenes serotypes from poultry meat and poultry preparations in Spain. Microorganisms, 7.","DOI":"10.3390\/microorganisms7110529"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Capita, R., Felices-Mercado, A., Garc\u00eda-Fern\u00e1ndez, C., and Alonso-Calleja, C. (2019). Characterization of Listeria monocytogenes originating from the Spanish meat-processing chain. Foods, 8.","DOI":"10.3390\/foods8110542"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"132","DOI":"10.1016\/j.lwt.2019.03.015","article-title":"Listeria monocytogenes survival in raw Atlantic salmon (Salmo salar) fillet under in vitro simulated gastrointestinal conditions by culture, qPCR and PMA-qPCR detection methods","volume":"107","author":"Barretta","year":"2019","journal-title":"LWT"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1080\/10408398.2010.519837","article-title":"Antibiotic-resistant bacteria: A challenge for the food industry","volume":"53","author":"Capita","year":"2013","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_19","unstructured":"O\u2019Neill, J. (2022, November 14). Antimicrobial Resistance: Tackling a Crisis for the Health and Wealth of Nations. Available online: https:\/\/amr-review.org\/sites\/default\/files\/AMR%20Review%20Paper%20-%20Tackling%20a%20crisis%20for%20the%20health%20and%20wealth%20of%20nations_1.pdf."},{"key":"ref_20","unstructured":"O\u2019Neill, J. (2021, September 22). Tackling Drug-Resistant Infections Globally: Final Report and Recommendations. Available online: https:\/\/amr-review.org\/sites\/default\/files\/160518_Final%20paper_with%20cover.pdf."},{"key":"ref_21","unstructured":"OCDE (2021, September 22). Antimicrobial Resistance. Tackling the Burden in the European Union., Available online: https:\/\/www.oecd.org\/health\/health-systems\/AMR-Tackling-the-Burden-in-the-EU-OECD-ECDC-Briefing-Note-2019.pdf."},{"key":"ref_22","first-page":"777","article-title":"Microbial load and antibiotic resistance in raw beef preparations from northwest Spain","volume":"8","author":"Capita","year":"2019","journal-title":"Food Sci. Nutr."},{"key":"ref_23","unstructured":"ICMSF (2011). Microorganisms in Foods 8. Use of Data for Assessing Process Control and Product Acceptance, Springer."},{"key":"ref_24","unstructured":"IFST (1997). Development and use of microbiological criteria for foods. Food Sci. Technol. Today, 11, 137\u2013176."},{"key":"#cr-split#-ref_25.1","unstructured":"OJEU (2005). Commission regulation"},{"key":"#cr-split#-ref_25.2","unstructured":"(EC) N\u00b0 2073\/2005 of 15 November 2005 on microbiological criteria for foodstuffs. Off. J. Eur. Union, L338, 1-26."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/j.ifset.2014.02.009","article-title":"Combined use of modified atmosphere packaging and high pressure to extend the shelf-life of raw poultry sausage","volume":"23","author":"Lerasle","year":"2014","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.psj.2020.09.066","article-title":"The effect of selected lactic acid bacterial strains on the technological and microbiological quality of mechanically separated poultry meat cured with a reduced amount of sodium nitrite","volume":"100","year":"2021","journal-title":"Poult. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/S0740-0020(03)00060-1","article-title":"Microbiological quality of vacuum-packed retail ostrich meat in Spain","volume":"21","author":"Prieto","year":"2004","journal-title":"Food Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"880","DOI":"10.1111\/j.1365-2621.2000.tb13604.x","article-title":"Prevalence of salmonellae on beef products at butcheries and their antibiotic resistance profiles","volume":"65","author":"Gashe","year":"2000","journal-title":"J. Food Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0168-1605(00)00497-9","article-title":"Occurrence of Listeria species in retail poultry meat and comparison of a cultural\/immunoassay for their detection","volume":"65","author":"Capita","year":"2001","journal-title":"Int. J. Food Microbiol."},{"key":"ref_31","unstructured":"Pascual-Anderson, M.R. (1992). Microbiolog\u00eda Alimentaria. Metodolog\u00eda Anal\u00edtica Para Alimentos Y Bebidas, Diaz de Santos."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Casta\u00f1o-Arriba, A., Gonz\u00e1lez-Machado, C., Igrejas, G., Poeta, P., Alonso-Calleja, C., and Capita, R. (2020). Antibiotic resistance and biofilm-forming ability in enterococcal isolates from red meat and poultry preparations. Pathogens, 9.","DOI":"10.3390\/pathogens9121021"},{"key":"ref_33","first-page":"103S","article-title":"The relationship between the phenotypic properties of bacteria from chill-stored meat and spoilage processes","volume":"73","author":"Dainty","year":"1992","journal-title":"J. Appl. Bacteriol. Symp. Suppl."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1111\/j.1365-2621.2002.tb10673.x","article-title":"Methods to detect the occurrence of various indicator bacteria on the surface of retail poultry in Spain","volume":"67","author":"Capita","year":"2002","journal-title":"J. Food Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.4315\/0362-028X-65.7.1200","article-title":"A Review of aerobic and psychrotrophic plate count procedures for fresh meat and poultry products","volume":"65","author":"Jay","year":"2002","journal-title":"J. Food Prot."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1016\/j.meatsci.2012.02.036","article-title":"The microbiological condition of minced pork prepared at retail stores in Athens, Greece","volume":"91","author":"Andritsos","year":"2012","journal-title":"Meat Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1108\/00070700410558184","article-title":"Enumeration of Campylobacter and Salmonella on chicken packs","volume":"106","author":"Wong","year":"2004","journal-title":"Br. Food J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1080\/00071660701821675","article-title":"Spoilage of light (PSE-like) and dark turkey meat under aerobic or modified atmosphere package: Microbial indicators and their relationship with total volatile basic nitrogen","volume":"49","author":"Fraqueza","year":"2008","journal-title":"Br. Poult. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.fm.2012.10.007","article-title":"Predominant enterobacteria on modified-atmosphere packaged meat and poultry","volume":"34","author":"Murros","year":"2013","journal-title":"Food Microbiol."},{"key":"ref_40","first-page":"1","article-title":"Validaci\u00f3n de PCR para Listeria monocytogenes en Leches","volume":"10","author":"Burbano","year":"2011","journal-title":"Aliment. Hoy"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"25","DOI":"10.19136\/era.a5n13.1428","article-title":"Standardization of PCR technique for detecting Listeria monocytogenes in chicken, beef and pork","volume":"5","year":"2018","journal-title":"Ecosistemas y Recur. Agropecu."},{"key":"ref_42","first-page":"61","article-title":"Estandarizaci\u00f3n de la extracci\u00f3n de ADN y validaci\u00f3n de la PCR m\u00faltiple para detectar Listeria monocytogenes en queso, leche, carne de res y pollo","volume":"10","author":"Poutou","year":"2005","journal-title":"Univ. Sci."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"7835253","DOI":"10.1155\/2019\/7835253","article-title":"Significance and characteristics of Listeria monocytogenes in poultry products","volume":"2019","author":"Jamshidi","year":"2019","journal-title":"Int. J. Food Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.foodcont.2011.06.006","article-title":"Increase over time in the prevalence of multiple antibiotic resistance among isolates of Listeria monocytogenes from poultry in Spain","volume":"23","author":"Prieto","year":"2012","journal-title":"Food Control."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.foodcont.2018.01.015","article-title":"Listeria monocytogenes at chicken slaughterhouse: Occurrence, genetic relationship among isolates and evaluation of antimicrobial susceptibility","volume":"88","author":"Oliveira","year":"2012","journal-title":"Food Control."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Gon\u00e7alves-Ten\u00f3rio, A., Nunes Silva, B., Rodrigues, V., Cadavez, V., and Gonzales-Barron, U. (2018). Prevalence of pathogens in poultry meat: A meta-analysis of European published surveys. Foods, 7.","DOI":"10.3390\/foods7050069"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1888","DOI":"10.4315\/0362-028X-65.12.1888","article-title":"Incidence of susceptibility to antimicrobial agents of Listeria spp. and Listeria monocytogenes isolated from poultry carcasses in Porto, Portugal","volume":"65","author":"Antunes","year":"2002","journal-title":"J. Food Prot."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0740-0020(03)00057-1","article-title":"Incidence of Listeria monocytogenes in different food products commercialized in Portugal","volume":"21","author":"Mena","year":"2004","journal-title":"Food Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.4315\/0362-028X.JFP-13-453","article-title":"Prevalence and antimicrobial susceptibility of Listeria monocytogenes on chicken carcasses in Bandung, Indonesia","volume":"77","author":"Sugiri","year":"2014","journal-title":"J. Food Prot."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0168-1605(91)90052-Q","article-title":"Listeria monocytogenes in foods in Norway","volume":"13","author":"Yndestad","year":"1991","journal-title":"Int. J. Food Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1111\/j.1472-765X.2004.01580.x","article-title":"Quantification of Listeria monocytogenes in fermented sausages by MPN-PCR method","volume":"39","author":"Garriga","year":"2004","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.ijfoodmicro.2007.11.006","article-title":"Detection quantification and vitality of Listeria monocytogenes in food as determined by quantitative PCR","volume":"21","author":"Rantsiou","year":"2008","journal-title":"Int. J. Food Microbiol."},{"key":"ref_53","unstructured":"AESAN (2021, September 21). Listeriosis. Available online: https:\/\/www.aesan.gob.es\/AECOSAN\/web\/seguridad_alimentaria\/subdetalle\/listeria.htm."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"6299","DOI":"10.1128\/AEM.70.10.6299-6301.2004","article-title":"Rapid quantitative detection of Listeria monocytogenes in meat products by Real-Time PCR","volume":"70","author":"Aymerich","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.4315\/0362-028X-68.7.1467","article-title":"Rapid quantitative detection of Listeria monocytogenes in salmon products: Evaluation of Pre\u2013Real-Time PCR strategies","volume":"68","author":"Aymerich","year":"2005","journal-title":"J. Food Prot."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.3168\/jds.2014-8938","article-title":"Propidium monoazide combined with real-time PCR for selective detection of viable Staphylococcus aureus in milk powder and meat products","volume":"98","author":"Zhang","year":"2015","journal-title":"J. Dairy Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.ijfoodmicro.2012.07.016","article-title":"Antimicrobial susceptibilities of Listeria monocytogenes strains isolated from food and food processing environment in Poland","volume":"158","author":"Korsak","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"946","DOI":"10.3389\/fmicb.2019.00946","article-title":"Isolation, potential virulence, and population diversity of Listeria monocytogenes from meat and meat products in China","volume":"10","author":"Chen","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1016\/j.bjm.2017.01.010","article-title":"Prevalence and serotype distribution of Listeria monocytogenes isolated from foods in Montevideo-Uruguay","volume":"48","author":"Braga","year":"2017","journal-title":"Braz. J. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"9066","DOI":"10.1038\/s41598-021-88514-y","article-title":"Environmental dissemination of pathogenic Listeria monocytogenes in fowing surface waters in Switzerland","volume":"11","author":"Raschle","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"551020","DOI":"10.3389\/fmicb.2020.551020","article-title":"10-year molecular surveillance of Listeria monocytogenes using whole-genome sequencing in Shanghai, China, 2009\u20132019","volume":"11","author":"Zhang","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Wu, S., Wu, Q., Zhang, J., Chen, M., Yan, Z., and Hu, H. (2015). Listeria monocytogenes prevalence and characteristics in retail foods in China. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0136682"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Lachtara, B., Wieczorek, K., and Osek, J. (2022). Genetic diversity and relationships of Listeria monocytogenes serogroup IIa isolated in Poland. Microorganisms, 10.","DOI":"10.3390\/microorganisms10030532"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1292\/jvms.15-0470","article-title":"Multiplex PCR serogrouping of Listeria monocytogenes isolated in Japan","volume":"78","author":"Shimojima","year":"2016","journal-title":"J. Vet. Med. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Varsaki, A., Ortiz, S., Santorum, P., L\u00f3pez, P., L\u00f3pez-Alonso, V., Hern\u00e1ndez, M., Abad, D., Rodr\u00edguez-Grande, J., Ocampo-Sosa, A.A., and Mart\u00ednez-Su\u00e1rez, J.V. (2022). Prevalence and population diversity of Listeria monocytogenes isolated from dairy cattle farms in the Cantabria Region of Spain. Animals, 12.","DOI":"10.3390\/ani12182477"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1016\/j.foodcont.2017.08.023","article-title":"Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco","volume":"84","author":"Amajoud","year":"2018","journal-title":"Food Control."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"109562","DOI":"10.1016\/j.ijfoodmicro.2022.109562","article-title":"Genetic diversity of Listeria monocytogenes strains contaminating food and food producing environment as single based sample in Italy (retrospective study)","volume":"366","author":"Acciari","year":"2022","journal-title":"Int. J. Food Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.foodcont.2012.05.014","article-title":"Prevalence and antimicrobial resistance patterns of Listeria species isolated from poultry products marketed in Iran","volume":"28","author":"Fallah","year":"2012","journal-title":"Food Control."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1277","DOI":"10.1111\/1541-4337.12387","article-title":"Emergence of antibiotic resistance in Listeria monocytogenes isolated from food products: A comprehensive review","volume":"17","author":"Olaimat","year":"2018","journal-title":"Compr. Rev. Food Sci. Food Saf."},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Cordero, J., Alonso-Calleja, C., Garc\u00eda-Fern\u00e1ndez, C., and Capita, R. (2019). Microbial load and antibiotic resistance patterns of Escherichia coli and Enterococcus faecalis isolates from the meat of wild and domestic pigeons. Foods, 8.","DOI":"10.3390\/foods8110536"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_72","unstructured":"WHO (2019). Critically Important Antimicrobials for Human Medicine."},{"key":"ref_73","unstructured":"OIE (2021, September 23). OIE List of Antimicrobial Agents of Veterinary Importance. Available online: http:\/\/www.oie.int\/fileadmin\/Home\/eng\/Our_scientific_expertise\/docs\/pdf\/Eng_OIE_List_antimicrobials_May2015.pdf."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"607","DOI":"10.1016\/j.foodcont.2012.12.016","article-title":"Prevalence, antibiotic resistance and genetic diversity of Listeria monocytogenes isolated from ducks, their rearing and processing environments in Penang, Malaysia","volume":"163","author":"Adzitey","year":"2013","journal-title":"Food Control."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.foodres.2014.07.049","article-title":"The sub-species characterization and antimicrobial resistance of Listeria monocytogenes isolated from domestic and imported food products from 2004 to 2011","volume":"64","author":"Bae","year":"2014","journal-title":"Food Res. Int."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1016\/j.foodcont.2014.06.043","article-title":"Prevalence and antimicrobial resistance of Listeria monocytogenes and Salmonella strains isolated in ready-to-eat foods in Eastern Spain","volume":"47","author":"Amoros","year":"2015","journal-title":"Food Control."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.ijfoodmicro.2017.01.013","article-title":"Survival of Listeria monocytogenes with different antibiotic resistance patterns to food-associated stresses","volume":"245","author":"Komora","year":"2017","journal-title":"Int. J. Food Microbiol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.foodcont.2012.11.032","article-title":"Occurrence and antimicrobial susceptibility of Listeria monocytogenes isolates from retail raw foods","volume":"32","author":"Wang","year":"2013","journal-title":"Food Control."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1136\/vr.102462","article-title":"Antibiotics used most commonly to treat animals in Europe","volume":"175","author":"Atkinson","year":"2014","journal-title":"Vet. Rec."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s40104-016-0127-3","article-title":"Antimicrobial usage and resistance in beef production","volume":"7","author":"Cameron","year":"2016","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3382\/ps\/pey539","article-title":"The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: A global overview","volume":"98","author":"Roth","year":"2019","journal-title":"Poult. Sci."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.ijfoodmicro.2011.11.011","article-title":"Prevalence and antimicrobial resistance of Salmonella serotypes isolates from poultry in Spain: Comparison between 1993 and 2006","volume":"153","author":"Capita","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1016\/j.jiph.2017.12.007","article-title":"Antibiotic susceptibility of 259 Listeria monocytogenes strains isolated from food, food-processing plants and human samples in Germany","volume":"11","author":"Noll","year":"2018","journal-title":"J. Infect. Public Health"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Downes, F.P., and Ito, K. (2001). Psychrotrophic microorganisms. Compendium of Methods for the Microbiological Examination of Foods, American Public Health Association. [4th ed.].","DOI":"10.2105\/9780875531755"},{"key":"ref_85","first-page":"221","article-title":"Pharmacopeia of culture media for food microbiology","volume":"5","author":"Baird","year":"1987","journal-title":"Int. J. Food Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1016\/j.foodcont.2013.01.048","article-title":"Simultaneous detection of Listeria species isolated from meat processed foods using multiplex PCR","volume":"32","author":"Ryu","year":"2013","journal-title":"Food Control"},{"key":"ref_87","first-page":"108609","article-title":"Prevalence, quantification and antibiotic resistance of Listeria monocytogenes in poultry preparations","volume":"135","author":"Capita","year":"2022","journal-title":"Food Control"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1126\/science.1063447","article-title":"Comparative genomics of Listeria species","volume":"294","author":"Glaser","year":"2001","journal-title":"Science"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"3819","DOI":"10.1128\/JCM.42.8.3819-3822.2004","article-title":"Differentiation of the major Listeria monocytogenes serovars by multiplex PCR","volume":"42","author":"Doumith","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_90","unstructured":"CLSI (2018). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Test for Bacteria Isolated from Animals, National Committee for Clinical Laboratory Standards. CLSI VET08-ED4:2018."},{"key":"ref_91","unstructured":"EUCAST (2021, September 03). European Committee on Antimicrobial Susceptibility Testing. Available online: https:\/\/www.eucast.org\/clinical_breakpoints\/."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/12\/1828\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:42:26Z","timestamp":1760146946000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/12\/1828"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,16]]},"references-count":92,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["antibiotics11121828"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11121828","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,16]]}}}