{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T16:18:09Z","timestamp":1791389889346,"version":"4.3.3"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2022,12,17]],"date-time":"2022-12-17T00:00:00Z","timestamp":1671235200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000865","name":"National Council for Science and Technological Development (CNPq) and the Bill &amp; Melinda Gates Foundation","doi-asserted-by":"publisher","award":["402659\/2018-0"],"award-info":[{"award-number":["402659\/2018-0"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"National Council for Science and Technological Development (CNPq) and the Bill &amp; Melinda Gates Foundation","doi-asserted-by":"publisher","award":["443805\/2018-0"],"award-info":[{"award-number":["443805\/2018-0"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"National Council for Science and Technological Development (CNPq) and the Bill &amp; Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1193112"],"award-info":[{"award-number":["OPP1193112"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"National Council for Science and Technological Development (CNPq) and the Bill &amp; Melinda Gates Foundation","doi-asserted-by":"publisher","award":["312066\/2019-8"],"award-info":[{"award-number":["312066\/2019-8"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["402659\/2018-0"],"award-info":[{"award-number":["402659\/2018-0"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["443805\/2018-0"],"award-info":[{"award-number":["443805\/2018-0"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["OPP1193112"],"award-info":[{"award-number":["OPP1193112"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["312066\/2019-8"],"award-info":[{"award-number":["312066\/2019-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>This study aimed to characterize a Klebsiella pneumoniae strain (KP411) recovered from the stool samples of poultry (Gallus gallus) in the Brazilian Amazon Region. The whole-genome sequencing of KP411 revealed the presence of an important arsenal of antimicrobial resistance genes to \u03b2-lactams (blaCTX-M-14, blaTEM-1B, blaKPC-2, blaSVH-11), aminoglycosides [aph(3\u2033)- Ib, aph(6)-Id, aph(3\u2032)-Ia], sulfonamides (sul1, sul2), quinolones (oqxAB), fosfomycin (fosAKP), and macrolides [mph(A)]. Furthermore, our analyses revealed that the KP411 strain belongs to the ST258 clonal lineage, which is one of the main epidemic clones responsible for the dissemination of KPC-2 worldwide. Our data suggest that food-producing animals may act as reservoirs of multidrug-resistant K. pneumoniae belonging to the ST258 clone, and, consequently, contribute to their dissemination to humans and the environment.<\/jats:p>","DOI":"10.3390\/antibiotics11121835","type":"journal-article","created":{"date-parts":[[2022,12,19]],"date-time":"2022-12-19T05:55:43Z","timestamp":1671429343000},"page":"1835","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Genomic Analysis of Klebsiella pneumoniae ST258 Strain Coproducing KPC-2 and CTX-M-14 Isolated from Poultry in the Brazilian Amazon Region"],"prefix":"10.3390","volume":"11","author":[{"given":"Tiago Barcelos","family":"Valiatti","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo","family":"Cay\u00f4","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ci\u00eancias Biol\u00f3gicas (DCB), Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas (ICAQF), Universidade Federal de S\u00e3o Paulo (UNIFESP), Diadema 09972-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda Fernandes","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Oz\u00f3rio","family":"Bessa-Neto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ci\u00eancias Biol\u00f3gicas (DCB), Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas (ICAQF), Universidade Federal de S\u00e3o Paulo (UNIFESP), Diadema 09972-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramon Giovani","family":"Brand\u00e3o Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"},{"name":"Laborat\u00f3rio de Imunologia e Microbiologia (LIB), Setor de Biologia Molecular, Microbiologia e Imunologia, Departamento de Ci\u00eancias Biol\u00f3gicas (DCB), Instituto de Ci\u00eancias Ambientais, Qu\u00edmicas e Farmac\u00eauticas (ICAQF), Universidade Federal de S\u00e3o Paulo (UNIFESP), Diadema 09972-270, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruanita","family":"Veiga","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia","family":"de Nazar\u00e9 Miranda Bahia","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00edvia Maria Guimar\u00e3es Dutra","family":"Guerra","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ant\u00f4nio Carlos Campos","family":"Pignatari","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Especial de Microbiologia Cl\u00ednica (LEMC), Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04025-010, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5496-3713","authenticated-orcid":false,"given":"Cintya","family":"de Oliveira Souza","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2772-639X","authenticated-orcid":false,"given":"Danielle Murici","family":"Brasiliense","sequence":"additional","affiliation":[{"name":"Se\u00e7\u00e3o de Bacteriologia e Micologia, Instituto Evandro Chagas (IEC), Ananindeua 67030-000, PA, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina","family":"Gales","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio Alerta, Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04039-032, SP, Brazil"},{"name":"Laborat\u00f3rio Especial de Microbiologia Cl\u00ednica (LEMC), Disciplina Infectologia, Departamento de Medicina, Escola Paulista de Medicina (EPM), Universidade Federal de S\u00e3o Paulo (UNIFESP), S\u00e3o Paulo 04025-010, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2022,12,17]]},"reference":[{"key":"ref_1","first-page":"858","article-title":"Surveillance of Antibiotic Use and Resistance in Intensive Care Units (SARI)","volume":"114","author":"Remschmidt","year":"2017","journal-title":"Dtsch. Arztebl. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"895","DOI":"10.3389\/fmicb.2016.00895","article-title":"Global dissemination of Carbapenemase-producing Klebsiella pneumoniae: Epidemiology, genetic context, treatment options, and detection methods","volume":"7","author":"Lee","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1016\/j.molmed.2012.03.003","article-title":"Carbapenem resistance in Enterobacteriaceae: Here is the storm!","volume":"18","author":"Nordmann","year":"2012","journal-title":"Trends Mol. Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of Carbapenemase-producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1093\/cid\/ciz824","article-title":"Epidemiology and Diagnostics of Carbapenem Resistance in Gram-negative Bacteria","volume":"69","author":"Nordmann","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"823684","DOI":"10.3389\/fcimb.2022.823684","article-title":"Global Threat of Carbapenem-Resistant Gram-Negative Bacteria","volume":"15","author":"Jean","year":"2022","journal-title":"Front. Cell Infect. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1111\/nyas.14223","article-title":"Plasmid evolution in carbapenemase-producing Enterobacteriaceae: A review","volume":"1457","author":"Kopotsa","year":"2019","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.1073\/pnas.1503141112","article-title":"Global trends in antimicrobial use in food animals","volume":"112","author":"Brower","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"18377","DOI":"10.1007\/s11356-018-1852-2","article-title":"Antimicrobial use and antimicrobial resistance in food animals","volume":"25","author":"Xiong","year":"2018","journal-title":"Environ. Sci. Pollut. Res. Int."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1086\/499968","article-title":"Genetic Determinants of Capsular Serotype K1 of Klebsiella pneumoniae Causing Primary Pyogenic Liver Abscess","volume":"193","author":"Chuang","year":"2006","journal-title":"J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"3446","DOI":"10.1099\/mic.0.050336-0","article-title":"The role of Klebsiella pneumoniae rmpA in capsular polysaccharide synthesis and virulence revisited","volume":"157","author":"Hsu","year":"2011","journal-title":"Microbiology"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1016\/j.ijid.2010.01.007","article-title":"Hypermucoviscosity associated with Klebsiella pneumoniae-mediated invasive syndrome: A prospective cross-sectional study in Taiwan","volume":"14","author":"Lee","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4344","DOI":"10.1177\/0300060519863544","article-title":"The pathogenicity of rmpA or aerobactin-positive Klebsiella pneumoniae in infected mice","volume":"47","author":"Li","year":"2019","journal-title":"J. Int. Med. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/cid\/ciz1095","article-title":"An Emerging Clone, Klebsiella pneumoniae Carbapenemase 2-Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting","volume":"71","author":"Andrey","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1089\/mdr.2019.0166","article-title":"Population Structure of KPC-2-Producing Klebsiella pneumoniae Isolated from Surveillance Rectal Swabs in Brazil","volume":"26","author":"Aires","year":"2020","journal-title":"Microb. Drug. Resist."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"e0155221","DOI":"10.1128\/AAC.01552-21","article-title":"Small IncQ1 Plasmid Encoding KPC-2 Expands to Invasive Nontyphoidal Salmonella","volume":"65","author":"Fontana","year":"2021","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5873","DOI":"10.1128\/AAC.01019-15","article-title":"Carbapenemase-Producing Klebsiella pneumoniae, a Key Pathogen Set for Global Nosocomial Dominance","volume":"59","author":"Pitout","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3579","DOI":"10.1128\/AAC.01783-10","article-title":"Dissemination of blaKPC-2 by the spread of Klebsiella pneumoniae clonal complex 258 clones (ST258, ST11, ST437) and plasmids (IncFII, IncN, IncL\/M) among Enterobacteriaceae species in Brazil","volume":"55","author":"Andrade","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1080\/21505594.2017.1279778","article-title":"Endotoxin neutralization by an O-antigen specific monoclonal antibody: A potential novel therapeutic approach against Klebsiella pneumoniae ST258","volume":"8","author":"Guachalla","year":"2017","journal-title":"Virulence"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2729","DOI":"10.2147\/IDR.S219155","article-title":"High rate of multiresistant Klebsiella pneumoniae from human and animal origin","volume":"12","author":"Yang","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1007\/s00284-013-0398-2","article-title":"Molecular characterization of an atypical IncX3 plasmid pKPC-NY79 carrying blaKPC-2 in a Klebsiella pneumoniae","volume":"67","author":"Ho","year":"2013","journal-title":"Curr. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/j.ijantimicag.2014.10.006","article-title":"Emergence of blaKPC carbapenemase genes in Australia","volume":"45","author":"Partridge","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"7674","DOI":"10.1038\/s41598-018-26073-5","article-title":"Genomic and functional characterisation of IncX3 plasmids encoding blaSHV -12 in Escherichia coli from human and animal origin","volume":"8","author":"Liakopoulos","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3453","DOI":"10.1128\/JB.00286-11","article-title":"Type 3 fimbriae and biofilm formation are regulated by the transcriptional regulators MrkHI in Klebsiella pneumoniae","volume":"193","author":"Johnson","year":"2011","journal-title":"J. Bacteriol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1099\/00222615-19-1-61","article-title":"Contribution of the traT gene to serum resistance among clinical isolates of Enterobacteriaceae","volume":"19","author":"Kanukollu","year":"1985","journal-title":"J. Med. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1128\/iai.28.2.359-367.1980","article-title":"Plasmid-determined resistance to serum bactericidal activity: A major outer membrane protein, the traT gene product, is responsible for plasmid-specified serum resistance in Escherichia coli","volume":"28","author":"Moll","year":"1980","journal-title":"Infect. Immun."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e0056522","DOI":"10.1128\/spectrum.00565-22","article-title":"Exploring the Bacteriome and Resistome of Humans and Food-Producing Animals in Brazil","volume":"10","author":"Carvalho","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"697","DOI":"10.1084\/jem.20030857","article-title":"A Novel Virulence Gene in Klebsiella pneumoniae Strains Causing Primary Liver Abscess and Septic Metastatic Complications","volume":"199","author":"Fang","year":"2004","journal-title":"J. Exp. Med."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A flexible trimmer for Illumina sequence data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2068","DOI":"10.1093\/bioinformatics\/btu153","article-title":"Prokka: Rapid prokaryotic genome annotation","volume":"30","author":"Seemann","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1093\/nar\/gkl858","article-title":"PATRIC: The VBI PathoSystems Resource Integration Center","volume":"35","author":"Snyder","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1093\/bioinformatics\/btl529","article-title":"Interactive Tree Of Life (iTOL): An online tool for phylogenetic tree display and annotation","volume":"23","author":"Letunic","year":"2007","journal-title":"Bioinformatics"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/12\/1835\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:43:17Z","timestamp":1760146997000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/11\/12\/1835"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,17]]},"references-count":33,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2022,12]]}},"alternative-id":["antibiotics11121835"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics11121835","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,12,17]]}}}