{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:54:43Z","timestamp":1792803283566,"version":"4.5.0"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073940"],"award-info":[{"award-number":["82073940"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82104306"],"award-info":[{"award-number":["82104306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2021JJ40815"],"award-info":[{"award-number":["2021JJ40815"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2022RC1229"],"award-info":[{"award-number":["2022RC1229"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21B0013"],"award-info":[{"award-number":["21B0013"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Hunan Provincial Natural Science Foundation of China","award":["82073940"],"award-info":[{"award-number":["82073940"]}]},{"name":"Hunan Provincial Natural Science Foundation of China","award":["82104306"],"award-info":[{"award-number":["82104306"]}]},{"name":"Hunan Provincial Natural Science Foundation of China","award":["2021JJ40815"],"award-info":[{"award-number":["2021JJ40815"]}]},{"name":"Hunan Provincial Natural Science Foundation of China","award":["2022RC1229"],"award-info":[{"award-number":["2022RC1229"]}]},{"name":"Hunan Provincial Natural Science Foundation of China","award":["21B0013"],"award-info":[{"award-number":["21B0013"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["82073940"],"award-info":[{"award-number":["82073940"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["82104306"],"award-info":[{"award-number":["82104306"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2021JJ40815"],"award-info":[{"award-number":["2021JJ40815"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["2022RC1229"],"award-info":[{"award-number":["2022RC1229"]}]},{"name":"Science and Technology Innovation Program of Hunan Province","award":["21B0013"],"award-info":[{"award-number":["21B0013"]}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department","award":["82073940"],"award-info":[{"award-number":["82073940"]}],"id":[{"id":"https:\/\/ror.org\/026meb765","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department","award":["82104306"],"award-info":[{"award-number":["82104306"]}],"id":[{"id":"https:\/\/ror.org\/026meb765","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department","award":["2021JJ40815"],"award-info":[{"award-number":["2021JJ40815"]}],"id":[{"id":"https:\/\/ror.org\/026meb765","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department","award":["2022RC1229"],"award-info":[{"award-number":["2022RC1229"]}],"id":[{"id":"https:\/\/ror.org\/026meb765","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Scientific Research Fund of Hunan Provincial Education Department","award":["21B0013"],"award-info":[{"award-number":["21B0013"]}],"id":[{"id":"https:\/\/ror.org\/026meb765","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Objectives: Conduction of pharmacokinetic (PK) study in pediatric patients is challenging due to blood sampling limits. The dried blood spots (DBS) method represents a potential matrix for microsampling in support of PK studies in children. Herein, we used the Capitainer\u00ae qDBS device to develop a DBS method that can collect an exact 10 \u00b5L volume of blood on a paper card. This DBS method was developed to simultaneously quantify the concentrations of eight antibiotics, including sulbactam, tazobactam, ampicillin, meropenem, cefotaxime, cefoperazone, piperacillin, and metronidazole using ultra-high performance liquid chromatography\u2013tandem mass spectrometry (UHPLC\u2013MS\/MS). Methods: The prepared DBS samples were extracted in methanol containing acetaminophen as the internal standard at 20 \u00b0C on a block bath shaker at 500 rpm for 30 min. The extracted antibiotics were eluted on an Acquity UPLC HSS T3 column (2.1 \u00d7 50 mm, 1.8 \u00b5m) using gradient elution with a total chromatographic run time of 6.5 min. The precursor and product ions of the analytes were detected by use of the multiple reaction monitoring (MRM) mode. Results: No interfering peaks at the respective retention times of the analytes were observed in DBS samples. The lower limits of quantification (LLOQ) for the antibiotics were between 0.25 and 2.0 \u03bcg\/mL, and satisfactory accuracies (intra\/inter-assay bias \u221216.7 to +13.6%) and precisions (intra\/inter-assay coefficient of variations 1.5\u201315.6%) were obtained for the analytes. As a proof of concept, the method was applied to DBS samples obtained from neonatal patients treated with ampicillin and piperacillin\/sulbactam. Conclusions: The DBS method is simple and robust, and it can be used in children with limited blood sampling.<\/jats:p>","DOI":"10.3390\/antibiotics12020199","type":"journal-article","created":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T02:31:11Z","timestamp":1674009071000},"page":"199","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["A Validated UHPLC\u2013MS\/MS Method to Quantify Eight Antibiotics in Quantitative Dried Blood Spots in Support of Pharmacokinetic Studies in Neonates"],"prefix":"10.3390","volume":"12","author":[{"given":"Qian","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lanyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yuan","sequence":"additional","affiliation":[{"name":"Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifan","family":"Xie","sequence":"additional","affiliation":[{"name":"Division of Biopharmaceutics and Pharmacokinetics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410013, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1002\/cpt.2180","article-title":"Variation in Target Attainment of Beta-Lactam Antibiotic Dosing Between International Pediatric Formularies","volume":"109","author":"Gastine","year":"2021","journal-title":"Clin. 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