{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T06:57:59Z","timestamp":1782284279043,"version":"3.54.5"},"reference-count":72,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,1,18]],"date-time":"2023-01-18T00:00:00Z","timestamp":1674000000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Deputyship for Research &amp; Innovation, Ministry of Education in Saudi Arabia","award":["IFP-2020-107"],"award-info":[{"award-number":["IFP-2020-107"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Staphylococcus aureus (S. aureus) is a serious infection-causing pathogen in humans and animal. In particular, methicillin-resistant S. aureus (MRSA) is considered one of the major life-threatening pathogens due to its rapid resistance to several antibiotics in clinical practice. MRSA strains have recently been isolated in a number of animals utilized in food production processes, and these species are thought to be the important sources of the spread of infection and disease in both humans and animals. The main objective of the current study was to assess the prevalence of drug-resistant S. aureus, particularly vancomycin-resistant S. aureus (VRSA) and MRSA, by molecular methods. To address this issue, a total of three hundred samples (200 meat samples from cattle and sheep carcasses (100 of each), 50 hand swabs, and 50 stool samples from abattoir workers) were obtained from slaughterhouses in Egypt provinces. In total, 19% S. aureus was isolated by standard culture techniques, and the antibiotic resistance was confirmed genotypically by amplification nucA gen. Characteristic resistance genes were identified by PCR with incidence of 31.5%, 19.3%, 8.7%, and 7% for the mecA, VanA, ermA, and tet L genes, respectively, while the aac6-aph gene was not found in any of the isolates. In this study, the virulence genes responsible for S. aureus\u2019 resistance to antibiotics had the highest potential for infection or disease transmission to animal carcasses, slaughterhouse workers, and meat products.<\/jats:p>","DOI":"10.3390\/antibiotics12020205","type":"journal-article","created":{"date-parts":[[2023,1,19]],"date-time":"2023-01-19T02:51:37Z","timestamp":1674096697000},"page":"205","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Molecular Basis of Methicillin and Vancomycin Resistance in Staphylococcus aureus from Cattle, Sheep Carcasses and Slaughterhouse Workers"],"prefix":"10.3390","volume":"12","author":[{"given":"Hanan A.","family":"Zaher","sequence":"first","affiliation":[{"name":"Food Hygiene and Control Department, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shimaa","family":"El Baz","sequence":"additional","affiliation":[{"name":"Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulaziz S.","family":"Alothaim","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman A.","family":"Alsalamah","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed Ibrahim","family":"Alghonaim","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullah S.","family":"Alawam","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University, Riyadh 11623, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6260-1229","authenticated-orcid":false,"given":"Mostafa M.","family":"Eraqi","sequence":"additional","affiliation":[{"name":"Department of Biology, College of Science in Zulfi, Majmaah University, Majmaah 11952, Saudi Arabia"},{"name":"Microbiology and Immunology Department, Veterinary Research Institute, National Research Centre, Dokki, Giza 12622, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,1,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"827965","DOI":"10.1155\/2014\/827965","article-title":"Staphylococcus aureus and Staphylococcal Food-Borne Disease: An Ongoing Challenge in Public Health","volume":"2014","author":"Kadariya","year":"2014","journal-title":"Biomed Res. Int."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1016\/j.meegid.2013.08.011","article-title":"Staphylococcus aureus in Veterinary Medicine","volume":"21","author":"Peton","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"3255","DOI":"10.2147\/IDR.S272733","article-title":"Methicillin-Resistant Staphylococcus aureus (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact","volume":"13","author":"Algammal","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nandhini, P., Kumar, P., Mickymaray, S., Alothaim, A.S., Somasundaram, J., and Rajan, M. (2022). Recent Developments in Methicillin-Resistant Staphylococcus aureus (MRSA) Treatment: A Review. Antibiotics, 11.","DOI":"10.3390\/antibiotics11050606"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"108901","DOI":"10.1016\/j.ijfoodmicro.2020.108901","article-title":"Resistance Profiling and Molecular Characterization of Staphylococcus aureus Isolated from Goats in Korea","volume":"336","author":"Mechesso","year":"2021","journal-title":"Int. J. Food Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2497","DOI":"10.2147\/IDR.S175967","article-title":"Methicillin-Resistant Staphylococcus aureus: Livestock-Associated, Antimicrobial, and Heavy Metal Resistance. Infect","volume":"11","author":"Dweba","year":"2018","journal-title":"Drug Resist."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"606","DOI":"10.1017\/S0950268809991567","article-title":"Methicillin-Resistant Staphylococcus aureus (MRSA) in Food Production Animals","volume":"138","author":"Vanderhaeghen","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1080\/10286020.2013.771344","article-title":"Liquiritigenin Prevents Staphylococcus aureus-Mediated Lung Cell Injury via Inhibiting the Production of \u03b1-Hemolysin","volume":"15","author":"Dai","year":"2013","journal-title":"J. Asian Nat. Prod. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2014.11.020","article-title":"Genetic Diversity and Virulence Potential of Staphylococcus aureus Isolates from Raw and Processed Food Commodities in Shanghai","volume":"195","author":"Song","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3762","DOI":"10.3382\/ps\/pey335","article-title":"Erratum: Corrigendum to \u201cCharacteristics of the Antimicrobial Resistance of Staphylococcus aureus Isolated from Chicken Mean Produced by Different Integrated Broiler Operations in Korea\u201d (Poultry Science (2018) 97 3 (962\u2013969))","volume":"97","author":"Kim","year":"2018","journal-title":"Poult. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1128\/microbiolspec.AME-0011-2019","article-title":"Molecular Epidemiology of Infectious Zoonotic and Livestock Diseases","volume":"8","author":"Gebreyes","year":"2020","journal-title":"Microbiol. Spectr."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-Resistant, Extensively Drug-Resistant and Pandrug-Resistant Bacteria: An International Expert Proposal for Interim Standard Definitions for Acquired Resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1038\/nrmicro2200","article-title":"Waves of Resistance: Staphylococcus aureus in the Antibiotic Era","volume":"7","author":"Chambers","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"346","DOI":"10.3181\/00379727-49-13559","article-title":"Observations on Resistance of Staphylococcus aureus to Action of Tyrothricin","volume":"49","author":"Rammelkamp","year":"1942","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Lade, H., Joo, H.S., and Kim, J.S. (2022). Molecular Basis of Non-\u03b2-Lactam Antibiotics Resistance in Staphylococcus aureus. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101378"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.meegid.2014.04.005","article-title":"MRSA Pediatric Clone Expressing ermC plus lnuA Genes Causing Nosocomial Transmission and Healthcare Workers Colonization in a Neonatal Intensive Care Unit","volume":"25","author":"Faccone","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"910","DOI":"10.1097\/00006454-200210000-00005","article-title":"Prospective Comparison of Risk Factors and Demographic and Clinical Characteristics of Community-Acquired, Methicillin-Resistant versus Methicillin-Susceptible Staphylococcus aureus Infection in Children","volume":"21","author":"Sattler","year":"2002","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/j.micpath.2019.01.006","article-title":"Poultry as a Vector for Emerging Multidrug Resistant Enterococcus Spp.: First Report of Vancomycin (van) and the Chloramphenicol\u2013Florfenicol (Cat-Fex-Cfr) Resistance Genes from Pigeon and Duck Faeces","volume":"128","author":"Osman","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1128\/AAC.01124-10","article-title":"Novel Apramycin Resistance Gene apmA in Bovine and Porcine Methicillin-Resistant Staphylococcus aureus ST398 Isolates. Antimicrob","volume":"55","author":"Kadlec","year":"2011","journal-title":"Agents Chemother."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2930","DOI":"10.1128\/AEM.07487-11","article-title":"Genotypes, Exotoxin Gene Content, and Antimicrobial Resistance of Staphylococcus aureus Strains Recovered from Foods and Food Handlers","volume":"78","author":"Mendoza","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3581","DOI":"10.1128\/JCM.01727-12","article-title":"Genetic Characterization of a Vancomycin-Resistant Staphylococcus aureus Isolate from the Respiratory Tract of a Patient in a University Hospital in Northeastern Iran","volume":"50","author":"Azimian","year":"2012","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1016\/j.ijmm.2013.02.006","article-title":"The Diversity of Antimicrobial Resistance Genes among Staphylococci of Animal Origin","volume":"303","author":"Wendlandt","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1186\/s12879-019-4547-5","article-title":"Molecular Characteristics and Virulence Gene Profiles of Staphylococcus aureus Isolates in Hainan, China","volume":"19","author":"Li","year":"2019","journal-title":"BMC Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1032","DOI":"10.1128\/JCM.38.3.1032-1035.2000","article-title":"Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance","volume":"38","author":"Mehrotra","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4465","DOI":"10.1128\/JCM.41.9.4465-4467.2003","article-title":"Use of Multiplex PCR to Identify Staphylococcus aureus Adhesins Involved in Human Hematogenous Infections","volume":"41","author":"Tristan","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1128\/jb.167.3.975-980.1986","article-title":"Molecular Cloning of the Gene of a Penicillin-Binding Protein Supposed to Cause High Resistance to \u03b2-Lactam Antibiotics in Staphylococcus aureus","volume":"167","author":"Matsuhashi","year":"1986","journal-title":"J. Bacteriol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"483","DOI":"10.3412\/jsb.55.483","article-title":"A New Mobile Genetic Element, Staphylococcal Cassette Chromosome Mec, Encodes Methicillin Resistance in Staphylococcus aureus","volume":"55","author":"Itou","year":"2000","journal-title":"Nippon. Saikingaku Zasshi. Jpn. J. Bacteriol."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Vijayaraj, R., Altaff, K., Lakshmanan, G., Jayaprakashvel, M., Mickymaray, S., Gunapriya, R., Palanisamy, M., and Alothaim, A.S. (2022). Inhibitory Activity of Chitin, (2-Acetamido-2-Deoxy-Hexopyranose) against Penicillin-Binding Proteins of Staphylococcus aureus. Coatings, 12.","DOI":"10.3390\/coatings12121854"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1128\/AAC.28.3.397","article-title":"Role of an Altered Penicillin-Binding Protein in Methicillin- and Cephem-Resistant Staphylococcus aureus","volume":"28","author":"Utsui","year":"1985","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Lade, H., and Kim, J.S. (2021). Bacterial Targets of Antibiotics in Methicillin-Resistant Staphylococcus aureus. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040398"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e01216-19","DOI":"10.1128\/AAC.01216-19","article-title":"Antimicrobial Resistance in Methicillin-Resistant Staphylococcus aureus to Newer Antimicrobial Agents","volume":"63","author":"Watkins","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_32","first-page":"443","article-title":"Enzymology of Bacterial Resistance","volume":"8","author":"Fisher","year":"2010","journal-title":"Compr. Nat. Prod. II Chem. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4580","DOI":"10.1128\/AAC.00346-09","article-title":"VanA-Type Vancomycin-Resistant Staphylococcus aureus","volume":"53","author":"Courvalin","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3062","DOI":"10.1016\/j.bbamem.2015.04.014","article-title":"Structural Variations of the Cell Wall Precursor Lipid II in Gram-Positive Bacteria\u2014Impact on Binding and Efficacy of Antimicrobial Peptides","volume":"1848","author":"Sahl","year":"2015","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3039","DOI":"10.1099\/00221287-133-11-3039","article-title":"The AacA-AphD Gentamicin and Kanamycin Resistance Determinant of Tn4001 from Staphylococcus aureus: Expression and Nucleotide Sequence Analysis","volume":"133","author":"Rouch","year":"1987","journal-title":"Microbiology"},{"key":"ref_36","first-page":"546","article-title":"Gentamicin Resistance in Staphylococcus aureus Isolated in Japan","volume":"30","author":"Ubukata","year":"1982","journal-title":"Chemotherapy"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3115","DOI":"10.1128\/JCM.39.9.3115-3121.2001","article-title":"Identification of Aminoglycoside-Modifying Enzymes by Susceptibility Testing: Epidemiology of Methicillin-Resistant Staphylococcus aureus in Japan","volume":"39","author":"Ida","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1128\/AAC.44.2.231-238.2000","article-title":"Correlation between the Resistance Genotype Determined by Multiplex PCR Assays and the Antibiotic Susceptibility Patterns of Staphylococcus aureus and Staphylococcus epidermidis","volume":"44","author":"Martineau","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1128\/AAC.35.7.1267","article-title":"Bacterial Resistance to Macrolide, Lincosamide, and Streptogramin Antibiotics by Target Modification. Antimicrob","volume":"35","author":"Leclercq","year":"1991","journal-title":"Agents Chemother."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1111\/j.1365-2958.1990.tb00696.x","article-title":"Inducible Erythromycin Resistance in Staphlyococci Is Encoded by a Member of the ATP-binding Transport Super-gene Family","volume":"4","author":"Ross","year":"1990","journal-title":"Mol. Microbiol."},{"key":"ref_41","first-page":"76","article-title":"Distribution of Genes Encoding Tetracycline Resistance and Aminoglycoside Modifying Enzymes in Staphylococcus aureus Strains Isolated from a Burn Center","volume":"26","author":"Emaneini","year":"2013","journal-title":"Ann. Burn. Fire Disasters"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/j.ijantimicag.2005.06.013","article-title":"Investigation of Erythromycin and Tetracycline Resistance Genes in Methicillin-Resistant Staphylococci","volume":"26","author":"Ardic","year":"2005","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.ijfoodmicro.2012.07.025","article-title":"Prevalence of Antibiotic Resistance in Coagulase-Negative Staphylococci from Spontaneously Fermented Meat Products and Safety Assessment for New Starters","volume":"159","author":"Marty","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1016\/j.ijbiomac.2018.11.005","article-title":"Synthesis, Characterization and Biomedical Applications of a Novel Schiff Base on Methyl Acrylate-Functionalized Chitosan Bearing p-Nitrobenzaldehyde Groups","volume":"122","author":"Kenawy","year":"2019","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1016\/j.ijfoodmicro.2011.12.032","article-title":"The Antibiotic Resistance Characteristics of Non-Typhoidal Salmonella enterica Isolated from Food-Producing Animals, Retail Meat and Humans in South East Asia","volume":"154","author":"Van","year":"2012","journal-title":"Int. J. Food Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3201\/eid1601.081467","article-title":"Public Health Threat of New, Reemerging, and Neglected Zoonoses in the Industrialized World","volume":"16","author":"Cutler","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"127","DOI":"10.3389\/fmicb.2012.00127","article-title":"Methicillin-Resistant Staphylococcus aureus Associated with Animals and Its Relevance to Human Health","volume":"3","author":"Pantosti","year":"2012","journal-title":"Front. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"222","DOI":"10.3389\/fmicb.2016.00222","article-title":"Antimicrobial Resistance, Biofilm Formation and mecA Characterization of Methicillin-Susceptible S. aureus and Non-S. aureus of Beef Meat Origin in Egypt","volume":"7","author":"Osman","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.foodcont.2018.08.030","article-title":"Prevalence and Characterization of Staphylococcus aureus and Staphylococcus argenteus in Chicken from Retail Markets in China","volume":"96","author":"Li","year":"2019","journal-title":"Food Control."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1089\/fpd.2017.2339","article-title":"Characterization of Staphylococcus aureus Isolated from Food Products in Western Algeria","volume":"15","author":"Chaalal","year":"2018","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1774","DOI":"10.4315\/0362-028X.JFP-11-479","article-title":"Antibiotic Resistance in Bacterial Pathogens from Retail Raw Meats and Food-Producing Animals in Japan","volume":"75","author":"Hiroi","year":"2012","journal-title":"J. Food Prot."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2157-7579.1000539","article-title":"Antimicrobial Resistance Profiles of Staphylococcus aureus Isolates along Asella Municipal Beef Abattoir Line, South Eastern Ethiopia","volume":"9","author":"Hassan","year":"2018","journal-title":"J. Vet. Sci. Technol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1459","DOI":"10.14202\/vetworld.2018.1459-1465","article-title":"Antibiotic Susceptibility Profile of Staphylococcus aureus Isolated from Sausages in Meknes, Morocco","volume":"11","author":"Filali","year":"2018","journal-title":"Vet. World"},{"key":"ref_54","first-page":"3","article-title":"Prevalence and Phenotypic Antibiotic Bioassay of Methicillin-Resistant Staphylococcus aureus in Raw Meats Sold at Various Retail Outlets in the Cape Coast Metropolis of Ghana","volume":"2","author":"Effah","year":"2018","journal-title":"Above Minim. Threshold. Again Retail. Outlet"},{"key":"ref_55","first-page":"49","article-title":"Bacteria Contamination of Raw Meat Sold in Abakaliki, Ebonyi State Nigeria","volume":"3","author":"Iroha","year":"2011","journal-title":"J. Public Health Epidemiol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Thwala, T., Madoroba, E., Basson, A., and Butaye, P. (2021). Prevalence and Characteristics of Staphylococcus aureus Associated with Meat and Meat Products in African Countries: A Review. Antibiotics, 10.","DOI":"10.3390\/antibiotics10091108"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"577707","DOI":"10.3389\/fmicb.2020.577707","article-title":"Methicillin-Resistant Staphylococcus aureus (MRSA) in Slaughter Houses and Meat Shops in Capital Territory of Pakistan During 2018\u20132019","volume":"11","author":"Sadiq","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"76","DOI":"10.21608\/mvmj.2021.76816.1061","article-title":"Characterization of Methicillin-Susceptible and Methicillin-Resistant Staphylococcus aureus from Healthy Cattle and Buffaloes in a Linked Community","volume":"22","author":"Gwida","year":"2021","journal-title":"Mansoura Vet. Med. J."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"463","DOI":"10.5455\/javar.2019.f369","article-title":"Prevalence and Phylogenetic Relationship among Methicillin- and Vancomycin-Resistant Staphylococci Isolated from Hospital\u2019s Dairy Food, Food Handlers, and Patients","volume":"6","author":"Mubarak","year":"2019","journal-title":"J. Adv. Vet. Anim. Res."},{"key":"ref_60","first-page":"82","article-title":"Vancomycin Resistance among Methicillin-Resistant Staphylococcus aureus Isolates from General Hospitals","volume":"10","author":"Ayoub","year":"2014","journal-title":"Kcmj"},{"key":"ref_61","first-page":"259","article-title":"Investigation of the Gene Carriage Rates for Staphylococcus aureus, MecA, VanA and Nuc Genes in the Nasal and Milk Specimens from the Sheep Caretakers with Sheep","volume":"27","author":"Akgul","year":"2021","journal-title":"Large Anim. Rev."},{"key":"ref_62","first-page":"1","article-title":"Vancomycin-Resistant Staphylococcus aureus Isolated from Camel Meat and Slaughterhouse Workers in Egypt","volume":"8","author":"Elhariri","year":"2019","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"630","DOI":"10.3201\/eid1304.060833","article-title":"MRSA Transmission between Cows and Humans","volume":"13","author":"Somogyi","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"975","DOI":"10.20546\/ijcmas.2018.702.121","article-title":"Occurrence and Distribution of Multiple Antibiotic Resistance Bacteria of Public Health Significance in Backwaters and Aquaculture Farm","volume":"7","author":"Pokhrel","year":"2018","journal-title":"Int. J. Curr. Microbiol. Appl. Sci."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"10969","DOI":"10.1007\/s11356-014-3887-3","article-title":"Multiple Antibiotic Resistance Indexing of Escherichia coli to Identify High-Risk Sources of Faecal Contamination of Water. Environ","volume":"22","author":"Titilawo","year":"2015","journal-title":"Sci. Pollut. Res."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"e12589","DOI":"10.1111\/jfs.12589","article-title":"Prevalence, Antimicrobial Susceptibility and Virulence Genes of Staphylococcus aureus Isolated from Pork Meat in Retail Outlets in India","volume":"39","author":"Savariraj","year":"2019","journal-title":"J. Food Saf."},{"key":"ref_67","doi-asserted-by":"crossref","unstructured":"Lozano, C., Gharsa, H., Ben Slama, K., Zarazaga, M., and Torres, C. (2016). Staphylococcus aureus in Animals and Food: Methicillin Resistance, Prevalence and Population Structure. a Review in the African Continent. Microorganisms, 4.","DOI":"10.3390\/microorganisms4010012"},{"key":"ref_68","unstructured":"International Organization for Standardization (2003). Microbiology of Food and Animal Feeding Stuffs\u2014Horizontal Method for the Enumeration of Coagulase-Positive Staphylococci (Staphylococcus aureus and Other Species), International Organization for Standardization. Available online: https:\/\/www.iso.org\/standard\/23036.html."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"M1291","DOI":"10.1111\/1750-3841.12893","article-title":"Species Diversity and Pheno- and Genotypic Antibiotic Resistance Patterns of Staphylococci Isolated from Retail Ground Meats","volume":"80","author":"Guran","year":"2015","journal-title":"J. Food Sci."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.clinmicnews.2019.11.002","article-title":"What\u2019s New from the CLSI Subcommittee on Antimicrobial Susceptibility Testing M100, 29th Edition","volume":"41","author":"Abbey","year":"2019","journal-title":"Clin. Microbiol. Newsl."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Zaher, H.A., Nofal, M.I., Hendam, B.M., Elshaer, M.M., Alothaim, A.S., and Eraqi, M.M. (2021). Prevalence and Antibiogram of Vibrio parahaemolyticus and Aeromonas hydrophila in the Flesh of Nile Tilapia, with Special Reference to Their Virulence Genes Detected Using Multiplex Pcr Technique. Antibiotics, 10.","DOI":"10.3390\/antibiotics10060654"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s12088-008-0056-8","article-title":"Detection of Aerolysin Gene in Aeromonas Hydrophila Isolated from Fish and Pond Water","volume":"48","author":"Singh","year":"2008","journal-title":"Indian J. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/2\/205\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:09:16Z","timestamp":1760119756000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/2\/205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,1,18]]},"references-count":72,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["antibiotics12020205"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12020205","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,1,18]]}}}