{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T05:58:01Z","timestamp":1785563881677,"version":"3.56.0"},"reference-count":75,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T00:00:00Z","timestamp":1675814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Foundation Ireland (SFI)","award":["12\/RC\/2275_P2"],"award-info":[{"award-number":["12\/RC\/2275_P2"]}]},{"name":"Science Foundation Ireland (SFI)","award":["12\/RI\/2346 (3)"],"award-info":[{"award-number":["12\/RI\/2346 (3)"]}]},{"DOI":"10.13039\/501100001602","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["12\/RC\/2275_P2"],"award-info":[{"award-number":["12\/RC\/2275_P2"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["12\/RI\/2346 (3)"],"award-info":[{"award-number":["12\/RI\/2346 (3)"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"SFI Research Infrastructure Call 2012","doi-asserted-by":"publisher","award":["12\/RC\/2275_P2"],"award-info":[{"award-number":["12\/RC\/2275_P2"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001602","name":"SFI Research Infrastructure Call 2012","doi-asserted-by":"publisher","award":["12\/RI\/2346 (3)"],"award-info":[{"award-number":["12\/RI\/2346 (3)"]}],"id":[{"id":"10.13039\/501100001602","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The urgent need to combat antibiotic resistance and develop novel antimicrobial therapies has triggered studies on novel metal-based formulations. N-heterocyclic carbene (NHC) complexes coordinate transition metals to generate a broad range of anticancer and\/or antimicrobial agents, with ongoing efforts being made to enhance the lipophilicity and drug stability. The lead silver(I) acetate complex, 1,3-dibenzyl-4,5-diphenylimidazol-2-ylidene (NHC*) (SBC3), has previously demonstrated promising growth and biofilm-inhibiting properties. In this work, the responses of two structurally different bacteria to SBC3 using label-free quantitative proteomics were characterised. Multidrug-resistant Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) are associated with cystic fibrosis lung colonisation and chronic wound infections, respectively. SBC3 increased the abundance of alginate biosynthesis, the secretion system and drug detoxification proteins in P. aeruginosa, whilst a variety of pathways, including anaerobic respiration, twitching motility and ABC transport, were decreased in abundance. This contrasted the affected pathways in S. aureus, where increased DNA replication\/repair and cell redox homeostasis and decreased protein synthesis, lipoylation and glucose metabolism were observed. Increased abundance of cell wall\/membrane proteins was indicative of the structural damage induced by SBC3 in both bacteria. These findings show the potential broad applications of SBC3 in treating Gram-positive and Gram-negative bacteria.<\/jats:p>","DOI":"10.3390\/antibiotics12020348","type":"journal-article","created":{"date-parts":[[2023,2,8]],"date-time":"2023-02-08T03:01:45Z","timestamp":1675825305000},"page":"348","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Pseudomonas aeruginosa and Staphylococcus aureus Display Differential Proteomic Responses to the Silver(I) Compound, SBC3"],"prefix":"10.3390","volume":"12","author":[{"given":"Magdalena","family":"Piatek","sequence":"first","affiliation":[{"name":"SSPC Pharma Research Centre, Department of Biology, Maynooth University, W23 F2K8 Maynooth, Co. Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cillian","family":"O\u2019Beirne","sequence":"additional","affiliation":[{"name":"School of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoe","family":"Beato","sequence":"additional","affiliation":[{"name":"School of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8903-3137","authenticated-orcid":false,"given":"Matthias","family":"Tacke","sequence":"additional","affiliation":[{"name":"School of Chemistry, University College Dublin, D04 V1W8 Belfield, Dublin 4, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Kavanagh","sequence":"additional","affiliation":[{"name":"SSPC Pharma Research Centre, Department of Biology, Maynooth University, W23 F2K8 Maynooth, Co. Kildare, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"609459","DOI":"10.3389\/fmicb.2021.609459","article-title":"Futuristic Non-antibiotic Therapies to Combat Antibiotic Resistance: A Review","volume":"12","author":"Kumar","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_2","first-page":"277","article-title":"The Antibiotics Resistance Crisis Part 1: Causes and Threats","volume":"40","year":"2015","journal-title":"Pharm. Ther."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"4207","DOI":"10.1021\/jo400236f","article-title":"Overcoming resistance to \u03b2-Lactam antibiotics","volume":"78","author":"Worthington","year":"2013","journal-title":"J. Org. Chem."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"a025247","DOI":"10.1101\/cshperspect.a025247","article-title":"\u03b2-lactams and \u03b2-lactamase inhibitors: An overview","volume":"6","author":"Bush","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"3966","DOI":"10.1021\/acs.biochem.8b00495","article-title":"Peptidoglycan and Teichoic Acid Levels and Alterations in Staphylococcus aureus by Cell-Wall and Whole-Cell Nuclear Magnetic Resonance","volume":"57","author":"Romaniuk","year":"2018","journal-title":"Biochemistry"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"808","DOI":"10.1016\/j.bbapap.2008.11.005","article-title":"Outer membrane permeability and antibiotic resistance","volume":"1794","author":"Delcour","year":"2009","journal-title":"Biochim. Biophys. Acta-Proteins Proteom."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.ijmm.2011.10.001","article-title":"Structure and function of OprD protein in Pseudomonas aeruginosa: From antibiotic resistance to novel therapies","volume":"302","author":"Li","year":"2012","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Jubeh, B., Breijyeh, Z., and Karaman, R. (2020). Resistance of gram-positive bacteria to current antibacterial agents and overcoming approaches. Molecules, 25.","DOI":"10.3390\/molecules25122888"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3389\/fmicb.2010.00134","article-title":"A brief history of the antibiotic era: Lessons learned and challenges for the future","volume":"1","author":"Aminov","year":"2010","journal-title":"Front. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2499","DOI":"10.3390\/molecules15042499","article-title":"Synthesis and antimicrobial activity of novel Ag-N-Heterocyclic carbene complexes","volume":"15","year":"2010","journal-title":"Molecules"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Prencipe, F., Zanfardino, A., Di Napoli, M., Rossi, F., D\u2019errico, S., Piccialli, G., Mangiatordi, G.F., Saviano, M., Ronga, L., and Varcamonti, M. (2021). Silver (I) n-heterocyclic carbene complexes: A winning and broad spectrum of antimicrobial properties. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22052497"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3859","DOI":"10.1021\/cr800500u","article-title":"The medicinal applications of imidazolium carbene-metal complexes","volume":"109","author":"Hindi","year":"2009","journal-title":"Chem. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"mfaa011","DOI":"10.1093\/mtomcs\/mfaa011","article-title":"Continuous flow synthesis and antimicrobial evaluation of NHC* silver carboxylate derivatives of SBC3 in vitro and in vivo","volume":"13","author":"Piatek","year":"2021","journal-title":"Metallomics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1093","DOI":"10.1002\/cbic.202000707","article-title":"The Antibacterial Drug Candidate SBC3 is a Potent Inhibitor of Bacterial Thioredoxin Reductase","volume":"22","author":"Napolitano","year":"2021","journal-title":"ChemBioChem"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1089\/sur.2008.9941","article-title":"History of the medical use of silver","volume":"10","author":"Alexander","year":"2009","journal-title":"Surg. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1089\/sur.2011.097","article-title":"Use of silver in the prevention and treatment of infections: Silver review","volume":"14","author":"Politano","year":"2013","journal-title":"Surg. Infect."},{"key":"ref_17","first-page":"1523","article-title":"Proteomics applications in health: Biomarker and drug discovery and food industry","volume":"17","author":"Koushki","year":"2018","journal-title":"Iran. J. Pharm. Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/j.idc.2016.02.004","article-title":"Multidrug-Resistant Bacteria in the Community: Trends and Lessons Learned","volume":"30","author":"Paterson","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1093\/biohorizons\/hzn008","article-title":"Gene expression of Pseudomonas aeruginosa and MRSA within a catheter-associated urinary tract infection biofilm model","volume":"1","author":"Goldsworthy","year":"2008","journal-title":"Biosci. Horiz."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"14849","DOI":"10.1038\/s41598-020-71834-w","article-title":"Bidirectional alterations in antibiotics susceptibility in Staphylococcus aureus\u2014Pseudomonas aeruginosa dual-species biofilm","volume":"10","author":"Trizna","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"672975","DOI":"10.3389\/fmicb.2021.672975","article-title":"Surfaceome and Exoproteome Dynamics in Dual-Species Pseudomonas aeruginosa and Staphylococcus aureus Biofilms","volume":"12","author":"Reigada","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Turner, R.J. (2018). Is silver the ultimate antimicrobial bullet?. Antibiotics, 7.","DOI":"10.3390\/antibiotics7040112"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1831","DOI":"10.3389\/fmicb.2016.01831","article-title":"Mechanistic basis of antimicrobial actions of silver nanoparticles","volume":"7","author":"Dakal","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"3100","DOI":"10.1021\/bi801750u","article-title":"Characterization of peptidoglycan in fem-deletion mutants of methicillin-resistant staphylococcus aureus by solid-state NMR","volume":"48","author":"Sharif","year":"2009","journal-title":"Biochemistry"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"100","DOI":"10.3389\/fmicb.2019.00100","article-title":"Deprivation of the Periplasmic Chaperone SurA Reduces Virulence and Restores Antibiotic Susceptibility of Multidrug-Resistant Pseudomonas aeruginosa","volume":"10","author":"Klein","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.3389\/fmicb.2018.02153","article-title":"The role of outer membrane proteins and lipopolysaccharides for the sensitivity of escherichia coli to antimicrobial peptides","volume":"9","author":"Ebbensgaard","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_27","unstructured":"Farhana, A., and Khan, Y.S. (2020). Biochemistry, Lipopolysaccharide, StatPearls Publishing."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1128\/JB.05230-11","article-title":"Surface-localized spermidine protects the Pseudomonas aeruginosa: Outer membrane from antibiotic treatment and oxidative stress","volume":"194","author":"Johnson","year":"2012","journal-title":"J. Bacteriol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5395","DOI":"10.1128\/JB.183.18.5395-5401.2001","article-title":"Alginate overproduction affects pseudomonas aeruginosa biofilm structure and function","volume":"183","author":"Hentzer","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00086-22","DOI":"10.1128\/jb.00086-22","article-title":"Pseudomonas aeruginosa Initiates a Rapid and Specific Transcriptional Response during Surface Attachment","volume":"204","author":"Jones","year":"2022","journal-title":"J. Bacteriol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7563","DOI":"10.1073\/pnas.1502025112","article-title":"Type IV pili mechanochemically regulate virulence factors in Pseudomonas aeruginosa","volume":"112","author":"Persat","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1128\/IAI.70.1.1-4.2002","article-title":"Type IV pilus-dependent motility and its possible role in bacterial pathogenesis","volume":"70","author":"Shi","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"10723","DOI":"10.1021\/nn5044383","article-title":"Nanoscale adhesion forces of Pseudomonas aeruginosa type IV pili","volume":"8","author":"Beaussart","year":"2014","journal-title":"ACS Nano"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"2126","DOI":"10.1128\/JB.00032-13","article-title":"PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA","volume":"195","author":"Tammam","year":"2013","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1074\/jbc.M114.616904","article-title":"Pseudomonas aeruginosa minor pilins prime type IVa pilus assembly and promote surface display of the PilY1 adhesin","volume":"290","author":"Nguyen","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Marko, V.A., Kilmury, S.L.N., MacNeil, L.T., and Burrows, L.L. (2018). Pseudomonas aeruginosa type IV minor pilins and PilY1 regulate virulence by modulating FimS-AlgR activity. PLoS Pathog., 14.","DOI":"10.1371\/journal.ppat.1007074"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1038\/nrmicro2199","article-title":"The type III secretion system of Pseudomonas aeruginosa: Infection by injection","volume":"7","author":"Hauser","year":"2009","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1128\/CMR.00013-07","article-title":"Type III secretion systems and disease","volume":"20","author":"Coburn","year":"2007","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"148","DOI":"10.3389\/fpls.2019.00418","article-title":"Molecular evolution of Pseudomonas syringae type iii secreted effector proteins","volume":"10","author":"Dillon","year":"2019","journal-title":"Front. Plant Sci."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Rangel, S.M., Diaz, M.H., Knoten, C.A., Zhang, A., and Hauser, A.R. (2015). The Role of ExoS in Dissemination of Pseudomonas aeruginosa during Pneumonia. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1005163"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e0238122","DOI":"10.1128\/mbio.02381-22","article-title":"PopB-PcrV Interactions Are Essential for Pore Formation in the Pseudomonas aeruginosa Type III Secretion System Translocon","volume":"13","author":"Emma","year":"2022","journal-title":"MBio"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1046\/j.1365-2958.2002.03228.x","article-title":"ExsD is a negative regulator of the Pseudomonas aeruginosa type III secretion regulon","volume":"46","author":"McCaw","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"442","DOI":"10.3389\/fcimb.2017.00442","article-title":"Identification and characterization of an antibacterial type VI secretion System in the carbapenem-resistant strain Klebsiella pneumoniae HS11286","volume":"7","author":"Liu","year":"2017","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e991","DOI":"10.1002\/mbo3.991","article-title":"Type VI secretion system of Pseudomonas aeruginosa is associated with biofilm formation but not environmental adaptation","volume":"9","author":"Chen","year":"2020","journal-title":"Microbiologyopen"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S1534-5807(02)00295-2","article-title":"Pseudomonas aeruginosa anaerobic respiration in biofilms: Relationships to cystic fibrosis pathogenesis","volume":"3","author":"Yoon","year":"2002","journal-title":"Dev. Cell"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Masamba, P., and Kappo, A.P. (2021). Parasite survival and disease persistence in cystic fibrosis, schistosomiasis and pathogenic bacterial diseases: A role for universal stress proteins?. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms221910878"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7189","DOI":"10.1128\/JB.00600-08","article-title":"SpoT-triggered stringent response controls usp gene expression in Pseudomonas aeruginosa","volume":"190","author":"Boes","year":"2008","journal-title":"J. Bacteriol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1719","DOI":"10.1111\/j.1462-2920.2010.02252.x","article-title":"Anaerobic adaptation in Pseudomonas aeruginosa: Definition of the Anr and Dnr regulons","volume":"12","author":"Trunk","year":"2010","journal-title":"Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Arat, S., Bullerjahn, G.S., and Laubenbacher, R. (2015). A network biology approach to denitrification in Pseudomonas aeruginosa. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0118235"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3246","DOI":"10.1016\/j.jmb.2019.05.046","article-title":"Crystal Structure of Dihydro-Heme d1 Dehydrogenase NirN from Pseudomonas aeruginosa Reveals Amino Acid Residues Essential for Catalysis","volume":"431","author":"Adamczack","year":"2019","journal-title":"J. Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"26754","DOI":"10.1074\/jbc.M111.239855","article-title":"Crystal structure of the heme d 1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferases","volume":"286","author":"Storbeck","year":"2011","journal-title":"J. Biol. Chem."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"30753","DOI":"10.1074\/jbc.M114.603886","article-title":"NirN protein from Pseudomonas aeruginosa is a novel electron-bifurcating dehydrogenase catalyzing the last step of Heme d1 biosynthesis","volume":"289","author":"Adamczack","year":"2014","journal-title":"J. Biol. Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1128\/jb.179.1.235-242.1997","article-title":"Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: Sequencing and identification of a locus for heme d1 biosynthesis","volume":"179","author":"Kawasaki","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4310","DOI":"10.1128\/JB.00240-07","article-title":"The anaerobic regulatory network required for Pseudomonas aeruginosa nitrate respiration","volume":"189","author":"Schreiber","year":"2007","journal-title":"J. Bacteriol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"30","DOI":"10.3389\/fmicb.2018.00030","article-title":"Substrate binding protein DppA1 of ABC transporter DppBCDF increases biofilm formation in Pseudomonas aeruginosa by inhibiting Pf5 prophage lysis","volume":"9","author":"Lee","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"e01188-18","DOI":"10.1128\/mBio.01188-18","article-title":"Branching out: Alterations in bacterial physiology and virulence due to branched-chain amino acid deprivation","volume":"9","author":"Kaiser","year":"2018","journal-title":"MBio"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.cmet.2016.12.022","article-title":"Multi-dimensional Roles of Ketone Bodies in Fuel Metabolism, Signaling, and Therapeutics","volume":"25","author":"Puchalska","year":"2017","journal-title":"Cell Metab."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"31876","DOI":"10.1038\/srep31876","article-title":"The ABC transporter YejABEF is required for resistance to antimicrobial peptides and the virulence of Brucella melitensis","volume":"6","author":"Wang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"16521","DOI":"10.1038\/s41598-017-16497-w","article-title":"Dynamics of Intact MexAB-OprM Efflux Pump: Focusing on the MexA-OprM Interface","volume":"7","author":"Travers","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1574-6968.1998.tb13122.x","article-title":"Functional replacement of OprJ by OprM in the MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa","volume":"165","author":"Gotoh","year":"1998","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wright, B.W., Kamath, K.S., Krisp, C., and Molloy, M.P. (2019). Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress. BMC Microbiol., 19.","DOI":"10.1186\/s12866-019-1441-7"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"17067","DOI":"10.1038\/nmicrobiol.2017.67","article-title":"Convergent evolution of a modified, acetate-driven TCA cycle in bacteria","volume":"2","author":"Kwong","year":"2017","journal-title":"Nat. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"7891","DOI":"10.1111\/febs.16234","article-title":"Cellular resource allocation strategies for cell size and shape control in bacteria","volume":"289","author":"Serbanescu","year":"2021","journal-title":"FEBS J."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"a025361","DOI":"10.1101\/cshperspect.a025361","article-title":"Bacterial protein synthesis as a target for antibiotic inhibition","volume":"6","author":"Arenz","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"mfac046","DOI":"10.1093\/mtomcs\/mfac046","article-title":"Exposure of Candida parapsilosis to the silver(I) compound SBC3 induces alterations in the proteome and reduced virulence","volume":"14","author":"Piatek","year":"2022","journal-title":"Metallomics"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.cbpa.2017.11.003","article-title":"Protein lipoylation: An evolutionarily conserved metabolic regulator of health and disease","volume":"42","author":"Rowland","year":"2018","journal-title":"Curr. Opin. Chem. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"e2022720118","DOI":"10.1073\/pnas.2022720118","article-title":"Staphylococcus aureus adapts to the host nutritional landscape to overcome tissue-specific branched-chain fatty acid requirement","volume":"118","author":"Teoh","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.chom.2017.09.004","article-title":"A Lipoylated Metabolic Protein Released by Staphylococcus aureus Suppresses Macrophage Activation","volume":"2","author":"Grayczyk","year":"2017","journal-title":"Cell Host Microbe"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"150","DOI":"10.1111\/mmi.13970","article-title":"Increased flexibility in the use of exogenous lipoic acid by Staphylococcus aureus","volume":"109","author":"Laczkovich","year":"2018","journal-title":"Mol. Microbiol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"381","DOI":"10.3389\/fchem.2019.00381","article-title":"Discovery of potential anti-infective therapy targeting glutamine synthetase in Staphylococcus xylosus","volume":"7","author":"Cui","year":"2019","journal-title":"Front. Chem."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"7193","DOI":"10.1039\/C9SC02032B","article-title":"Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis of: E. coli","volume":"10","author":"Wang","year":"2019","journal-title":"Chem. Sci."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"3331","DOI":"10.1038\/s41467-021-23659-y","article-title":"Multi-target mode of action of silver against Staphylococcus aureus endows it with capability to combat antibiotic resistance","volume":"12","author":"Wang","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1038\/s41467-021-21718-y","article-title":"Staphylococcus aureus induces an itaconate-dominated immunometabolic response that drives biofilm formation","volume":"12","author":"Tomlinson","year":"2021","journal-title":"Nat. Commun."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1074\/mcp.RA120.002059","article-title":"The Aspergillus fumigatus secretome alters the proteome of Pseudomonas aeruginosa to stimulate bacterial growth: Implications for co-infection","volume":"19","author":"Margalit","year":"2020","journal-title":"Mol. Cell. 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