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However, the underlying molecular mechanism responsible for the hVISA\/VISA phenotype is complex and not yet fully understood. We have previously characterized two ST100-MRSA-hVISA clinical isolates recovered before and after 40 days of vancomycin treatment (D1 and D2, respectively) and two in vitro VISA derivatives (D23C9 and D2P11), selected independently from D2 in the presence of vancomycin. This follow-up study was aimed at further characterizing these isogenic strains and obtaining their whole genome sequences to unravel changes associated with antibiotic resistance. It is interesting to note that none of these isogenic strains carry SNPs in the regulatory operons vraUTSR, walKR and\/or graXRS. Nonetheless, genetic changes including SNPs, INDELs and IS256 genomic insertions\/rearrangements were found both in in vivo and in vitro vancomycin-selected strains. Some were found in the downstream target genes of the aforementioned regulatory operons, which are involved in cell wall and phosphate metabolism, staphylococcal growth and biofilm formation. Some of the genetic changes reported herein have not been previously associated with vancomycin, daptomycin and\/or oxacillin resistance in S. aureus.<\/jats:p>","DOI":"10.3390\/antibiotics12020372","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T03:28:46Z","timestamp":1676258926000},"page":"372","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Comparative Genomics Identifies Novel Genetic Changes Associated with Oxacillin, Vancomycin and Daptomycin Susceptibility in ST100 Methicillin-Resistant Staphylococcus aureus"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-3318","authenticated-orcid":false,"given":"Sabrina","family":"Di Gregorio","sequence":"first","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-1996","authenticated-orcid":false,"given":"Mar\u00eda Sol","family":"Haim","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"},{"name":"Unidad Operativa Centro Nacional de Gen\u00f3mica y Bioinform\u00e1tica, ANLIS Dr. Carlos G. Malbr\u00e1n, Ciudad Aut\u00f3noma de Buenos Aires 1282, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngela Mar\u00eda Rosa","family":"Famiglietti","sequence":"additional","affiliation":[{"name":"Laboratorio de Bacteriolog\u00eda Cl\u00ednica, Hospital de Cl\u00ednicas Jos\u00e9 de San Mart\u00edn, Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0520-1744","authenticated-orcid":false,"given":"Jos\u00e9","family":"Di Conza","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5735-8471","authenticated-orcid":false,"given":"Marta","family":"Mollerach","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Bacteriolog\u00eda y Virolog\u00eda Molecular (IBaViM), Facultad de Farmacia y Bioqu\u00edmica, Universidad de Buenos Aires, Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"},{"name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas (CONICET), Ciudad Aut\u00f3noma de Buenos Aires 1113, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,2,11]]},"reference":[{"key":"ref_1","unstructured":"(2022, December 20). 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