{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T16:17:18Z","timestamp":1784132238262,"version":"3.55.0"},"reference-count":110,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2023,2,26]],"date-time":"2023-02-26T00:00:00Z","timestamp":1677369600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Government of the Russian Federation","award":["075-15-2022-1124"],"award-info":[{"award-number":["075-15-2022-1124"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>LF3872 was isolated from the milk of a healthy lactating and breastfeeding woman. Earlier, the genome of LF3872 was sequenced, and a gene encoding unique bacteriocin was discovered. We have shown here that the LF3872 strain produces a novel thermolabile class III bacteriolysin (BLF3872), exhibiting antimicrobial activity against antibiotic-resistant Staphylococcus aureus strains. Sequence analysis revealed the two-domain structural (lysozyme-like domain and peptidase M23 domain) organization of BLF3872. At least 25% residues of this protein are expected to be intrinsically disordered. Furthermore, BLF3872 is predicted to have a very high liquid-liquid phase separation. According to the electron microscopy data, the bacterial cells of LF3872 strain form co-aggregates with the S. aureus 8325-4 bacterial cells. LF3872 produced bacteriolysin BLF3872 that lyses the cells of the S. aureus 8325-4 mastitis-inducing strain. The sensitivity of the antibiotic-resistant S. aureus collection strains and freshly isolated antibiotic-resistant strains was tested using samples from women with lactation mastitis; the human nasopharynx and oral cavity; the oropharynx of pigs; and the cows with a diagnosis of clinical mastitis sensitive to the lytic action of the LF3872 strain producing BLF3872. The co-cultivation of LF3872 strain with various antibiotic-resistant S. aureus strains for 24 h reduced the level of living cells of these pathogens by six log. The LF3872 strain was found to be able to co-aggregate with all studied S. aureus strains. The cell-free culture supernatant of LF3872 (CSLF3872) induced S. aureus cell damage and ATP leakage. The effectiveness of the bacteriolytic action of LF3872 strain did not depend on the origin of the S. aureus strains. The results reported here are important for the creation of new effective drugs against antibiotic-resistant strains of S. aureus circulating in humans and animals.<\/jats:p>","DOI":"10.3390\/antibiotics12030471","type":"journal-article","created":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T03:29:48Z","timestamp":1677468588000},"page":"471","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Limosilactobacillus fermentum 3872 That Produces Class III Bacteriocin Forms Co-Aggregates with the Antibiotic-Resistant Staphylococcus aureus Strains and Induces Their Lethal Damage"],"prefix":"10.3390","volume":"12","author":[{"given":"Vyacheslav M.","family":"Abramov","sequence":"first","affiliation":[{"name":"Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution \u201cThe Russian State Center for Animal Feed and Drug Standardization and Quality\u201d (FGBU VGNKI), 123022 Moscow, Russia"},{"name":"Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor V.","family":"Kosarev","sequence":"additional","affiliation":[{"name":"Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution \u201cThe Russian State Center for Animal Feed and Drug Standardization and Quality\u201d (FGBU VGNKI), 123022 Moscow, Russia"},{"name":"Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4859-7219","authenticated-orcid":false,"given":"Andrey V.","family":"Machulin","sequence":"additional","affiliation":[{"name":"Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center \u201cPushchino Scientific Center for Biological Research of Russian Academy of Science\u201d, Russian Academy of Science, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana V.","family":"Priputnevich","sequence":"additional","affiliation":[{"name":"Kulakov National Medical Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Health, 117997 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgenia I.","family":"Deryusheva","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, Federal Research Center \u201cPushchino Scientific Center for Biological Research of Russian Academy of Science\u201d, Russian Academy of Science, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina L.","family":"Nemashkalova","sequence":"additional","affiliation":[{"name":"Institute for Biological Instrumentation, 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Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander N.","family":"Panin","sequence":"additional","affiliation":[{"name":"Federal Service for Veterinary and Phytosanitary Surveillance (Rosselkhoznadzor) Federal State Budgetary Institution \u201cThe Russian State Center for Animal Feed and Drug Standardization and Quality\u201d (FGBU VGNKI), 123022 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0825-7930","authenticated-orcid":false,"given":"Vyacheslav G.","family":"Melnikov","sequence":"additional","affiliation":[{"name":"Gabrichevsky Research Institute for Epidemiology and Microbiology, 125212 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nataliya E.","family":"Suzina","sequence":"additional","affiliation":[{"name":"Skryabin Institute of Biochemistry and Physiology of Microorganisms, Federal Research Center \u201cPushchino Scientific Center for Biological Research of Russian Academy of Science\u201d, Russian Academy of Science, 142290 Pushchino, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9495-0178","authenticated-orcid":false,"given":"Ilia N.","family":"Nikonov","sequence":"additional","affiliation":[{"name":"Federal State Educational Institution of Higher Professional Education, Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. Skryabin, 109472 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina V.","family":"Selina","sequence":"additional","affiliation":[{"name":"Federal State Educational Institution of Higher Professional Education, Moscow State Academy of Veterinary Medicine and Biotechnology named after K.I. 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Fam. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1016\/j.meegid.2013.08.018","article-title":"Staphylococcus aureus: A pathogen with still unresolved issues","volume":"21","author":"Rasigade","year":"2014","journal-title":"Infect. Genet. Evol."},{"key":"ref_3","unstructured":"Mandell, G.L., Bennett, J.E., and Dolin, R. (2000). Bennett\u2019s-Principles and Practice of Infectious Diseases, Churchill Livingstone."},{"key":"ref_4","unstructured":"Prescott, J.F., Songer, J.G., and Thoen, C.O. (2010). Pathogenesis of Bacterial Infections in Animals, Wiley-Blackwell."},{"key":"ref_5","first-page":"611","article-title":"Appropriate prescribing of oral beta-lactam antibiotics","volume":"62","author":"Holten","year":"2000","journal-title":"Am. Fam. Physician"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"a025247","DOI":"10.1101\/cshperspect.a025247","article-title":"\u03b2-Lactams and \u03b2-Lactamase Inhibitors: An Overview","volume":"6","author":"Bush","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"833189","DOI":"10.3389\/fphar.2022.833189","article-title":"Pharmacodynamic Thresholds for Beta-Lactam Antibiotics: A Story of Mouse Versus Man","volume":"13","author":"Berry","year":"2022","journal-title":"Front. Pharmacol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"015","DOI":"10.30574\/gscarr.2021.9.2.0200","article-title":"Beta-lactam antibiotics and extended spectrum beta-lactamases","volume":"9","author":"Silago","year":"2021","journal-title":"GSC Adv. Res. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4471","DOI":"10.1007\/s00018-016-2302-2","article-title":"Morphological and ultrastructural changes in bacterial cells as an indicator of antibacterial mechanism of action","volume":"73","author":"Cushnie","year":"2016","journal-title":"Cell. Mol. Life. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.cmrp.2018.01.001","article-title":"Methicillin-resistant Staphylococcus aureus: An update on the epidemiology, treatment options and infection control","volume":"8","author":"Boswihi","year":"2018","journal-title":"Curr. Med. Res. Pract."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1265","DOI":"10.1172\/JCI18535","article-title":"Antimicrobial resistance: The example of Staphylococcus aureus","volume":"111","author":"Lowy","year":"2003","journal-title":"J. Clin. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3057","DOI":"10.1007\/s00018-010-0389-4","article-title":"Mobile genetic elements of Staphylococcus aureus","volume":"67","author":"Malachowa","year":"2010","journal-title":"Cell Mol. Life Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"255","DOI":"10.3201\/eid1302.060924","article-title":"Methicillin-resistant Staphylococcus aureus ST398 in Humans and Animals, Central Europe","volume":"13","author":"Witte","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1093\/jac\/dkm416","article-title":"Does antibiotic exposure increase the risk of methicillin-resistant Staphylococcus aureus (MRSA) isolation? A systematic review and meta-analysis","volume":"61","author":"Tacconelli","year":"2008","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1007\/s10156-010-0045-9","article-title":"Community-acquired methicillin-resistant Staphylococcus aureus: Community transmission, pathogenesis, and drug resistance","volume":"16","author":"Yamamoto","year":"2010","journal-title":"J. Infect. Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17916","DOI":"10.1038\/s41598-018-36206-5","article-title":"Comparison of community- and healthcare-associated methicillin-resistant Staphylococcus aureus isolates at a Chinese tertiary hospital, 2012\u20132017","volume":"8","author":"Peng","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1159\/000354201","article-title":"Community-Acquired Methicillin-Resistant Staphylococcus aureus: The New Face of an Old Foe?","volume":"22","author":"Udo","year":"2013","journal-title":"Med. Princ. Pract."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"18889","DOI":"10.1038\/s41598-020-76009-1","article-title":"Presence of egc-positive major clones ST 45, 30 and 22 among methicillin-resistant and methicillin-susceptible oral Staphylococcus aureus strains","volume":"10","author":"Kwapisz","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1086\/338568","article-title":"Risk Factors for the Transmission of Methicillin-Resistant Staphylococcus aureus in an Adult Intensive Care Unit: Fitting a Model to the Data","volume":"185","author":"Grundmann","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2976","DOI":"10.1001\/jama.290.22.2976","article-title":"Comparison of Community- and Health Care\u2013Associated Methicillin-Resistant Staphylococcus aureus Infection","volume":"290","author":"Naimi","year":"2003","journal-title":"JAMA"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1128\/JCM.44.1.108-118.2006","article-title":"Characterization of a Strain of Community-Associated Methicillin-Resistant Staphylococcus aureus Widely Disseminated in the United States","volume":"44","author":"Tenover","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"7687","DOI":"10.1073\/pnas.122108599","article-title":"The evolutionary history of methicillin-resistant Staphylococcus aureus (MRSA)","volume":"99","author":"Enright","year":"2002","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1093\/jac\/dkf009","article-title":"Risk factors associated with nosocomial methicillin-resistant Staphylococcus aureus (MRSA) infection including previous use of antimicrobials","volume":"49","author":"Graffunder","year":"2002","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"4289","DOI":"10.1128\/JCM.40.11.4289-4294.2002","article-title":"Dissemination of New Methicillin-Resistant Staphylococcus aureus Clones in the Community","volume":"40","author":"Okuma","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3926","DOI":"10.1128\/AAC.47.12.3926-3934.2003","article-title":"Evolutionary Models of the Emergence ofMethicillin-Resistant Staphylococcus aureus","volume":"47","author":"Robinson","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"978","DOI":"10.3201\/eid0908.030089","article-title":"Community-Acquired Methicillin-Resistant Staphylococcus aureus Carrying Panton-Valentine Leukocidin Genes: Worldwide Emergence","volume":"9","author":"Vandenesch","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2637","DOI":"10.1128\/AAC.48.7.2637-2651.2004","article-title":"Novel Type V Staphylococcal Cassette Chromosome mec Driven by a Novel Cassette Chromosome Recombinase, ccrC","volume":"48","author":"Ito","year":"2004","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"479","DOI":"10.47102\/annals-acadmedsg.V35N7p479","article-title":"Community-associated methicillin-resistant Staphylococcus aureus: Overview and local situation","volume":"35","author":"Wijaya","year":"2006","journal-title":"Ann. Acad. Med. Singap."},{"key":"ref_29","unstructured":"WHO (2022, November 27). Publishes List of Bacteria for Which New Antibiotics Are Urgently Needed. Available online: https:\/\/www.who.int\/news\/item\/27-02-2017-who-publishes-list-of-bacteria-for-which-new-antibiotics-are-urgently-needed."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Garvey, M. (2020). Bacteriophages and the One Health Approach to Combat Multidrug Resistance: Is This the Way?. Antibiotics, 9.","DOI":"10.3390\/antibiotics9070414"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Zalewska-Pi\u0105tek, B., and Pi\u0105tek, R. (2021). Bacteriophages as Potential Tools for Use in Antimicrobial Therapy and Vaccine Development. Pharmaceuticals, 14.","DOI":"10.3390\/ph14040331"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Broncano-Lavado, A., Santamar\u00eda-Corral, G., Esteban, J., and Garc\u00eda-Quintanilla, M. (2021). Advances in Bacteriophage Therapy against Relevant MultiDrug-Resistant Pathogens. Antibiotics, 10.","DOI":"10.3390\/antibiotics10060672"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1111\/j.1399-302X.2007.00420.x","article-title":"Purification of bacteriocin LS1 produced by human oral isolate Lactobacillus salivarius BGHO1","volume":"23","author":"Busarcevic","year":"2008","journal-title":"Oral Microbiol. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1590\/S1517-83822012000400019","article-title":"In vitro antagonistic growth effects of Lactobacillus fermentum and Lactobacillus salivarius and their fermentative broth on periodontal pathogens","volume":"43","author":"Chen","year":"2012","journal-title":"Braz. J. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1086\/652763","article-title":"Treatment of Infectious Mastitis during Lactation: Antibiotics versus Oral Administration of Lactobacilli Isolated from Breast Milk","volume":"50","author":"Arroyo","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"169","DOI":"10.3920\/BM2013.0036","article-title":"Probiotics for human lactational mastitis","volume":"5","author":"Arroyo","year":"2014","journal-title":"Benef. Microbes"},{"key":"ref_37","first-page":"2169","article-title":"Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis","volume":"4","author":"Soleimani","year":"2010","journal-title":"African J. Microbiol. Res."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Abramov, V.M., Kosarev, I.V., Machulin, A.V., Priputnevich, T.V., Chikileva, I.O., Deryusheva, E.I., Abashina, T.N., Donetskova, A.D., Panin, A.N., and Melnikov, V.G. (2022). Limosilactobacillus fermentum Strain 3872: Antibacterial and Immunoregulatory Properties and Synergy with Prebiotics against Socially Significant Antibiotic-Resistant Infections of Animals and Humans. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101437"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2489","DOI":"10.1128\/iai.57.8.2489-2494.1989","article-title":"Roles of alpha-toxin and beta-toxin in virulence of Staphylococcus aureus for the mouse mammary gland","volume":"57","author":"Bramley","year":"1989","journal-title":"Infect. Immun."},{"key":"ref_40","first-page":"1","article-title":"Potential probiotic-associated traits revealed from completed high quality genome sequence of Lactobacillus fermentum 3872","volume":"12","author":"Lehri","year":"2017","journal-title":"Stand. Genom. Sci."},{"key":"ref_41","unstructured":"G\u00fcll\u00fcce, M., Karaday\u0131, M., and Bar\u0131\u015f, \u00d6. (2013). Microbial Pathogens and Strategies for Combating Them: Science, Technology and Education, Formatex Research Center."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1058","DOI":"10.1016\/j.idairyj.2005.10.026","article-title":"Bacteriocins: Biological tools for bio-preservation and shelf-life extension","volume":"16","author":"Deegan","year":"2006","journal-title":"Int. Dairy J."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1038\/nrmicro2937","article-title":"Bacteriocins\u2014A viable alternative to antibiotics?","volume":"11","author":"Cotter","year":"2013","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"D227","DOI":"10.1093\/nar\/gkp971","article-title":"MEROPS: The peptidase database","volume":"38","author":"Rawlings","year":"2010","journal-title":"Nucleic Acids Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"272","DOI":"10.3389\/fphar.2015.00272","article-title":"Bacteriocins as Potential Anticancer Agents","volume":"6","author":"Kaur","year":"2015","journal-title":"Front. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"9552","DOI":"10.1073\/pnas.0803787105","article-title":"Crystal and cryoEM structural studies of a cell wall degrading enzyme in the bacteriophage \u03c629 tail","volume":"105","author":"Xiang","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e4496","DOI":"10.1002\/pro.4496","article-title":"Rapid prediction and analysis of protein intrinsic disorder","volume":"31","author":"Dayhoff","year":"2022","journal-title":"Protein Sci."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Horvath, A., Miskei, M., Ambrus, V., Vendruscolo, M., and Fuxreiter, M. (2020). Sequence-based prediction of protein binding mode landscapes. PLOS Comput. Biol., 16.","DOI":"10.1371\/journal.pcbi.1007864"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1042\/EBC20220052","article-title":"Biological soft matter: Intrinsically disordered proteins in liquid\u2013liquid phase separation and biomolecular condensates","volume":"66","author":"Fonin","year":"2022","journal-title":"Essays Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00018-022-04276-4","article-title":"Liquid\u2013liquid phase separation as an organizing principle of intracellular space: Overview of the evolution of the cell compartmentalization concept","volume":"79","author":"Antifeeva","year":"2022","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Bliven, S.E., Lafita, A., Rose, P.W., Capitani, G., Prli\u0107, A., and Bourne, P.E. (2019). Analyzing the symmetrical arrangement of structural repeats in proteins with CE-Symm. PLOS Comput. Biol., 15.","DOI":"10.1371\/journal.pcbi.1006842"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"716","DOI":"10.1016\/j.tibs.2019.03.005","article-title":"Stochasticity of Biological Soft Matter: Emerging Concepts in Intrinsically Disordered Proteins and Biological Phase Separation","volume":"44","author":"Turoverov","year":"2019","journal-title":"Trends Biochem. Sci."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"e1700193","DOI":"10.1002\/pmic.201700193","article-title":"Intrinsically Disordered Proteome of Human Membrane-Less Organelles","volume":"18","author":"Darling","year":"2018","journal-title":"Proteomics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.sbi.2016.10.015","article-title":"Intrinsically disordered proteins in overcrowded milieu: Membrane-less organelles, phase separation, and intrinsic disorder","volume":"44","author":"Uversky","year":"2017","journal-title":"Curr. Opin. Struct. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/j.cis.2016.05.012","article-title":"Protein intrinsic disorder-based liquid\u2013liquid phase transitions in biological systems: Complex coacervates and membrane-less organelles","volume":"239","author":"Uversky","year":"2017","journal-title":"Adv. Colloid Interface Sci."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"15","DOI":"10.1016\/j.febslet.2014.11.028","article-title":"Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates","volume":"589","author":"Uversky","year":"2015","journal-title":"FEBS Lett."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Chu, X., Sun, T., Li, Q., Xu, Y., Zhang, Z., Lai, L., and Pei, J. (2022). Prediction of liquid\u2013liquid phase separating proteins using machine learning. BMC Bioinform., 23.","DOI":"10.1186\/s12859-022-04599-w"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"2461","DOI":"10.1021\/acs.biochem.2c00499","article-title":"Sequence-based Prediction of the Cellular Toxicity Associated with Amyloid Aggregation within Protein Condensates","volume":"61","author":"Horvath","year":"2022","journal-title":"Biochemistry"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"167201","DOI":"10.1016\/j.jmb.2021.167201","article-title":"Sequence Determinants of the Aggregation of Proteins Within Condensates Generated by Liquid-liquid Phase Separation","volume":"434","author":"Vendruscolo","year":"2022","journal-title":"J. Mol. Biol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1128\/MMBR.62.1.181-203.1998","article-title":"Growth of the Stress-Bearing and Shape-Maintaining Murein Sacculus of Escherichia coli","volume":"62","year":"1998","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1753","DOI":"10.1080\/21505594.2017.1362533","article-title":"Lactobacillus fermentum 3872 as a potential tool for combatting Campylobacter jejuni infections","volume":"8","author":"Lehri","year":"2017","journal-title":"Virulence"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Ferraboschi, P., Ciceri, S., and Grisenti, P. (2021). Applications of Lysozyme, an Innate Immune Defense Factor, as an Alternative Antibiotic. Antibiotics, 10.","DOI":"10.3390\/antibiotics10121534"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1007\/s12038-010-0015-5","article-title":"Lysozymes in the animal kingdom","volume":"35","author":"Callewaert","year":"2010","journal-title":"J. Biosci."},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Fischetti, V.A. (2018). Development of Phage Lysins as Novel Therapeutics: A Historical Perspective. Viruses, 10.","DOI":"10.3390\/v10060310"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Sutton, J.A.F., Carnell, O.T., Lafage, L., Gray, J., Biboy, J., Gibson, J.F., Pollitt, E.J.G., Tazoll, S.C., Turnbull, W., and Hajdamowicz, N.H. (2021). Staphylococcus aureus cell wall structure and dynamics during host-pathogen interaction. PLOS Pathog., 17.","DOI":"10.1371\/journal.ppat.1009468"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1111\/j.1574-695X.2011.00806.x","article-title":"Characterization ofStaphylococcus aureus strains involved in human and bovine mastitis","volume":"62","author":"Delgado","year":"2011","journal-title":"FEMS Immunol. Med Microbiol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"298","DOI":"10.3201\/eid1302.060989","article-title":"Postpartum Mastitis and Community-acquired Methicillin-resistant Staphylococcus aureus","volume":"13","author":"Reddy","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/S0749-0720(02)00079-8","article-title":"Management and treatment of staphylococcal mastitis","volume":"19","author":"Sears","year":"2003","journal-title":"Vet. Clin. North Am. Food Anim. Pract."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"384","DOI":"10.3389\/fped.2018.00384","article-title":"Maternal-Infant Correlation of Multidrug-Resistant Staphylococcus aureus Carriage: A Prospective Cohort Study","volume":"6","author":"Lin","year":"2018","journal-title":"Front. Pediatr."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Garbacz, K., Kwapisz, E., and Wierzbowska, M. (2019). Denture stomatitis associated with small-colony variants of Staphylococcus aureus: A case report. BMC Oral Health, 19.","DOI":"10.1186\/s12903-019-0910-6"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1097\/INF.0b013e31815819bb","article-title":"A Review of Community-Associated Methicillin-Resistant Staphylococcus aureus Skin and Soft Tissue Infections","volume":"27","author":"Gorwitz","year":"2008","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"e207","DOI":"10.1055\/s-0035-1563388","article-title":"Neonatal Presentation of an Air-Filled Neck Mass that Enlarges with Valsalva: A Case Report","volume":"5","author":"Patel","year":"2015","journal-title":"Am. J. Perinatol. Rep."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"1965","DOI":"10.3201\/eid1112.050428","article-title":"Methicillin-resistant Staphylococcus aureus in Pig Farming","volume":"11","author":"Voss","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.3201\/eid1409.071576","article-title":"Pigs as Source of Methicillin-Resistant Staphylococcus aureus CC398 Infections in Humans, Denmark","volume":"14","author":"Lewis","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.3201\/eid1507.080652","article-title":"Methicillin-Resistant Staphylococcus aureus ST398 in Swine Farm Personnel, Belgium","volume":"15","author":"Denis","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"19542","DOI":"10.2807\/ese.15.16.19542-en","article-title":"Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland, 2009","volume":"15","author":"Huber","year":"2010","journal-title":"Euro Surveill."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1017\/S0950268810000075","article-title":"Prevalence of livestock-associated MRSA in broiler flocks and risk factors for slaughterhouse personnel in The Netherlands","volume":"138","author":"Mulders","year":"2010","journal-title":"Epidemiol. Infect."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1800","DOI":"10.1017\/S0950268811002378","article-title":"MRSA-ST398 in livestock farmers and neighbouring residents in a rural area in Germany","volume":"140","author":"Bisdorff","year":"2012","journal-title":"Epidemiol. Infect."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1016\/j.vetmic.2011.09.010","article-title":"Quantification of transmission of livestock-associated methicillin resistant Staphylococcus aureus in pigs","volume":"155","author":"Broens","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Broens, E.M., Espinosa-Gongora, C., Graat, E.A., Vendrig, N., Van Der Wolf, P.J., Guardabassi, L., Butaye, P., Nielsen, J.P., De Jong, M.C., and Van De Giessen, A.W. (2012). Longitudinal study on transmission of MRSA CC398 within pig herds. BMC Vet. Res., 8.","DOI":"10.1186\/1746-6148-8-58"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1016\/j.vetmic.2007.07.021","article-title":"Transmission of methicillin-resistant Staphylococcus aureus strains between different kinds of pig farms","volume":"126","author":"Ikawaty","year":"2008","journal-title":"Vet. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1794","DOI":"10.1017\/S0950268811002391","article-title":"Farm-specific lineages of methicillin-resistant Staphylococcus aureus clonal complex 398 in Danish pig farms","volume":"140","author":"Larsen","year":"2012","journal-title":"Epidemiol. Infect."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1111\/j.1863-2378.2010.01340.x","article-title":"Longitudinal Investigation of Methicillin-Resistant Staphylococcus aureus in Piglets","volume":"58","author":"Weese","year":"2011","journal-title":"Zoonoses Public Health"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.vetmic.2012.09.014","article-title":"Cohort study for the presence of livestock-associated MRSA in piglets: Effect of sow status at farrowing and determination of the piglet colonization age","volume":"162","author":"Verhegghe","year":"2013","journal-title":"Vet. Microbiol."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1594","DOI":"10.1017\/S0950268810002888","article-title":"Experimental colonization of pigs with methicillin-resistant Staphylococcus aureus (MRSA): Insights into the colonization and transmission of livestock-associated MRSA","volume":"139","author":"Moodley","year":"2011","journal-title":"Epidemiol. Infect."},{"key":"ref_87","unstructured":"(2022, December 27). One Health. Available online: https:\/\/www.who.int\/health-topics\/one-health#tab=tab_1."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"981","DOI":"10.1046\/j.1365-2672.2003.01915.x","article-title":"Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92","volume":"94","author":"Kos","year":"2003","journal-title":"J. Appl. Microbiol."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"M89","DOI":"10.1111\/j.1750-3841.2007.00294.x","article-title":"Indigenous Dadih Lactic Acid Bacteria: Cell-Surface Properties and Interactions with Pathogens","volume":"72","author":"Collado","year":"2007","journal-title":"J. Food Sci."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"104420","DOI":"10.1016\/j.micpath.2020.104420","article-title":"Aggregation, adherence, anti-adhesion and antagonistic activity properties relating to surface charge of probiotic Lactobacillus brevis gp104 against Staphylococcus aureus","volume":"147","author":"Hojjati","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1007\/s12602-020-09659-2","article-title":"Probiotic Properties of Lactobacilli and Their Ability to Inhibit the Adhesion of Enteropathogenic Bacteria to Caco-2 and HT-29 Cells","volume":"13","author":"Fonseca","year":"2021","journal-title":"Probiotics Antimicrob. Proteins"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"1985","DOI":"10.1128\/IAI.66.5.1985-1989.1998","article-title":"Adherence of Human Vaginal Lactobacilli to Vaginal Epithelial Cells and Interaction with Uropathogens","volume":"66","author":"Boris","year":"1998","journal-title":"Infect. Immun."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1086\/318070","article-title":"Assessment of the Capacity of Lactobacillus to Inhibit the Growth of Uropathogens and Block Their Adhesion to Vaginal Epithelial Cells","volume":"183","author":"Osset","year":"2001","journal-title":"J. Infect. Dis."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"884","DOI":"10.1046\/j.1365-2672.2002.01759.x","article-title":"Characterization and selection of vaginal Lactobacillus strains for the preparation of vaginal tablets","volume":"93","author":"Mastromarino","year":"2002","journal-title":"J. Appl. Microbiol."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Younes, J.A., Van Der Mei, H.C., van den Heuvel, E., Busscher, H.J., and Reid, G. (2012). Adhesion Forces and Coaggregation between Vaginal Staphylococci and Lactobacilli. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0036917"},{"key":"ref_96","first-page":"357","article-title":"Bacteriocin production and resistance to drugs are advantageous features for Lactobacillus acidophilus La-14, a potential probiotic strain","volume":"34","author":"Todorov","year":"2011","journal-title":"New Microbiol."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1111\/jam.12459","article-title":"Evaluation of antipathogenic activity and adherence properties of human Lactobacillus strains for vaginal formulations","volume":"116","author":"Verdenelli","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/S0966-842X(02)00034-3","article-title":"Bacterial coaggregation: An integral process in the development of multi-species biofilms","volume":"11","author":"Rickard","year":"2003","journal-title":"Trends Microbiol."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/BF02094024","article-title":"Coaggregation of urogenital bacteria in vitro and in vivo","volume":"20","author":"Reid","year":"1990","journal-title":"Curr. Microbiol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/s10295-018-2008-6","article-title":"Class III bacteriocin Helveticin-M causes sublethal damage on target cells through impairment of cell wall and membrane","volume":"45","author":"Sun","year":"2018","journal-title":"J. Ind. Microbiol. Biotechnol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1128\/AEM.01631-09","article-title":"Biosynthesis and Degradation of H2O2 by Vaginal Lactobacilli","volume":"76","year":"2010","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/j.resmic.2017.04.001","article-title":"The role of lactic acid production by probiotic Lactobacillus species in vaginal health","volume":"168","author":"Tachedjian","year":"2017","journal-title":"Res. Microbiol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"129","DOI":"10.3920\/BM2014.0021","article-title":"Inhibition of Staphylococcus aureus by crude and fractionated extract from lactic acid bacteria","volume":"6","author":"Wong","year":"2015","journal-title":"Benef. Microbes"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/j.anireprosci.2005.11.004","article-title":"Inhibition of Staphylococcusaureus by H2O2-producing Lactobacillus gasseri isolated from the vaginal tract of cattle","volume":"96","author":"Otero","year":"2006","journal-title":"Anim. Reprod. Sci."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"01006-13","DOI":"10.1128\/genomeA.01006-13","article-title":"Draft Genome Sequence of Lactobacillus fermentum Strain 3872","volume":"1","author":"Karlyshev","year":"2013","journal-title":"Genome Announc."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"fnv068","DOI":"10.1093\/femsle\/fnv068","article-title":"Lactobacillus fermentum 3872 genome sequencing reveals plasmid and chromosomal genes potentially involved in a probiotic activity","volume":"362","author":"Lehri","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1385\/1-59259-584-7:531","article-title":"Protein Identification and Analysis Tools in the ExPASy Server","volume":"Volume 112","author":"Wilkins","year":"1999","journal-title":"2-D Proteome Analysis Protocols. Methods in Molecular Biology"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"33254","DOI":"10.1073\/pnas.2007670117","article-title":"Widespread occurrence of the droplet state of proteins in the human proteome","volume":"117","author":"Hardenberg","year":"2020","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"W337","DOI":"10.1093\/nar\/gkac386","article-title":"FuzDrop on AlphaFold: Visualizing the sequence-dependent propensity of liquid\u2013liquid phase separation and aggregation of proteins","volume":"50","author":"Hatos","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1146","DOI":"10.3168\/jds.S0022-0302(96)76468-8","article-title":"Growth Characteristics of Bifidobacteria in Infant Formulas","volume":"79","author":"Dubey","year":"1996","journal-title":"J. Dairy Sci."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/3\/471\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:42:50Z","timestamp":1760121770000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/3\/471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,2,26]]},"references-count":110,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2023,3]]}},"alternative-id":["antibiotics12030471"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12030471","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,2,26]]}}}