{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:23:36Z","timestamp":1793021016217,"version":"4.5.0"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,3,24]],"date-time":"2023-03-24T00:00:00Z","timestamp":1679616000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The World Health Organization has recently identified three categories of pathogens, namely: critical, high, and medium priority, according to the need for new antibiotics. Critical priority pathogens include carbapenem-resistant microorganism (CPO) such as A. baumannii and P. aeruginosa, K. pneumoniae, and Enterobacter spp., whereas vancomycin-resistant E. faecium (VRE), methicillin and vancomycin-resistant S. aureus (MRSA) are in the high priority list. We compared the trend of antimicrobial resistants (AMRs) in clinical isolates, divided by year and bacteria spp., of samples obtained from nosocomial and community patients. Patient records were collected, including age, sex, site of infection, isolated organisms, and drug susceptibility patterns. From 2019 to 2022, a total of 113,635 bacterial isolates were tested, of which 11,901 resulted in antimicrobial resistants. An increase in the prevalence of several antibiotics resistant bacteria was observed. Specifically, the percentage of CPO cases increased from 2.62% to 4.56%, the percentage of MRSA increased from 1.84% to 2.81%, and the percentage of VRE increased from 0.58% to 2.21%. AMRs trend resulted in increases in CPO and MRSA for both community and nosocomial. Our work aims to highlight the necessity of preventive and control measures to be adopted in order to reduce the spread of multidrug-resistant pathogens.<\/jats:p>","DOI":"10.3390\/antibiotics12040651","type":"journal-article","created":{"date-parts":[[2023,3,27]],"date-time":"2023-03-27T04:05:40Z","timestamp":1679889940000},"page":"651","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Trends in Antibiotic Resistance of Nosocomial and Community-Acquired Infections in Italy"],"prefix":"10.3390","volume":"12","author":[{"given":"Paola","family":"Cerini","sequence":"first","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca Rita","family":"Meduri","sequence":"additional","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"},{"name":"Microbiology and Virology Laboratory, Department of Molecular Medicine, University of Rome \u201cLa Sapienza\u201d, 00185 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6336-814X","authenticated-orcid":false,"given":"Flaminia","family":"Tomassetti","sequence":"additional","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"},{"name":"Biochemistry Laboratory, Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella","family":"Polidori","sequence":"additional","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Brugneti","sequence":"additional","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"},{"name":"Microbiology and Virology Laboratory, Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1415-9808","authenticated-orcid":false,"given":"Eleonora","family":"Nicolai","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Bernardini","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"},{"name":"Department of Laboratory Medicine, \u201cTor Vergata\u201d University Hospital, Viale Oxford 81, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3463-0268","authenticated-orcid":false,"given":"Massimo","family":"Pieri","sequence":"additional","affiliation":[{"name":"Biochemistry Laboratory, Department of Experimental Medicine, University of Rome \u201cTor Vergata\u201d, 00133 Rome, Italy"},{"name":"Department of Laboratory Medicine, \u201cTor Vergata\u201d University Hospital, Viale Oxford 81, 00133 Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Broccolo","sequence":"additional","affiliation":[{"name":"Cerba Healthcare Italia srl, Viale Roma 190\/A, Guidonia Montecelio, 00012 Rome, Italy"},{"name":"Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, 73100 Lecce, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,3,24]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (WHO) (2017). Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections, Including Tuberculosis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2857","DOI":"10.1080\/10408398.2015.1077192","article-title":"Antimicrobial Resistance: A Global Emerging Threat to Public Health Systems","volume":"57","author":"Ferri","year":"2017","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1179\/2047773215Y.0000000030","article-title":"Antimicrobial Resistance: A Global Multifaceted Phenomenon","volume":"109","author":"Prestinaci","year":"2015","journal-title":"Pathog. Glob. Health"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"421","DOI":"10.4161\/viru.21282","article-title":"Intrinsic and Acquired Resistance Mechanisms in Enterococcus","volume":"3","author":"Hollenbeck","year":"2012","journal-title":"Virulence"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"S122","DOI":"10.1038\/nm1145","article-title":"Antibacterial Resistance Worldwide: Causes, Challenges and Responses","volume":"10","author":"Levy","year":"2004","journal-title":"Nat. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1050","DOI":"10.1126\/science.257.5073.1050","article-title":"Epidemiology of Drug Resistance: Implications for a Post-Antimicrobial Era","volume":"257","author":"Cohen","year":"1992","journal-title":"Science"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e00181-19","DOI":"10.1128\/CMR.00181-19","article-title":"Antimicrobial Resistance in ESKAPE Pathogens","volume":"33","author":"Forde","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_8","unstructured":"Ecdc (2022). Antimicrobial Resistance Surveillance in Europe, 2022\u20132020 Data."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Suay-Garc\u00eda, B., and P\u00e9rez-Gracia, M.T. (2019). Present and Future of Carbapenem-Resistant Enterobacteriaceae (CRE) Infections. Antibiotics, 8.","DOI":"10.3390\/antibiotics8030122"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1016\/j.ijmm.2013.02.007","article-title":"Community-Associated MRSA: What Makes Them Special?","volume":"303","author":"Otto","year":"2013","journal-title":"Int. J. Med. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1016\/j.mib.2012.08.003","article-title":"Global Epidemiology of Community-Associated Methicillin Resistant Staphylococcus Aureus (CA-MRSA)","volume":"15","author":"Mediavilla","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_12","first-page":"135","article-title":"Predominance of Gram-Negative Bacilli and Increasing Antimicrobial Resistance in Nosocomial Bloodstream Infections at a University Hospital in Southern Taiwan, 1996\u20132003","volume":"39","author":"Wu","year":"2006","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1159\/000346352","article-title":"One-Year Surveillance of ESKAPE Pathogens in an Intensive Care Unit of Monterrey, Mexico","volume":"58","author":"Flores","year":"2012","journal-title":"Chemotherapy"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Katchanov, J., Asar, L., Klupp, E.-M., Both, A., Rothe, C., K\u00f6nig, C., Rohde, H., Kluge, S., and Maurer, F.P. (2018). Carbapenem-Resistant Gram-Negative Pathogens in a German University Medical Center: Prevalence, Clinical Implications and the Role of Novel \u03b2-Lactam\/\u03b2-Lactamase Inhibitor Combinations. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0195757"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"225","DOI":"10.33073\/pjm-2019-024","article-title":"Patterns of Drug-Resistant Bacteria in a General Hospital, China, 2011\u20132016","volume":"68","author":"Mao","year":"2019","journal-title":"Pol. J. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1086\/524891","article-title":"The Epidemic of Antibiotic-Resistant Infections: A Call to Action for the Medical Community from the Infectious Diseases Society of America","volume":"46","author":"Spellberg","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1016\/j.cll.2004.05.008","article-title":"Major Factors Affecting the Emergence and Re-Emergence of Infectious Diseases","volume":"24","author":"Church","year":"2004","journal-title":"Clin. Lab. Med."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Mustafai, M.M., Hafeez, M., Munawar, S., Basha, S., Rabaan, A.A., Halwani, M.A., Alawfi, A., Alshengeti, A., Najim, M.A., and Alwarthan, S. (2023). Prevalence of Carbapenemase and Extended-Spectrum \u03b2-Lactamase Producing Enterobacteriaceae: A Cross-Sectional Study. Antibiotics, 12.","DOI":"10.3390\/antibiotics12010148"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1111\/j.1469-0691.2012.03815.x","article-title":"Identification and Screening of Carbapenemase-Producing Enterobacteriaceae","volume":"18","author":"Nordmann","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"S8","DOI":"10.1016\/S0924-8579(10)70004-2","article-title":"Emerging Carbapenemases: A Global Perspective","volume":"36","author":"Walsh","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1016\/j.idc.2021.07.002","article-title":"Vancomycin-Resistant Enterococci: Epidemiology, Infection Prevention, and Control","volume":"35","author":"Joshi","year":"2021","journal-title":"Infect. Dis. Clin. N. Am"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/j.jmii.2022.08.003","article-title":"Trend in Healthcare-Associated Infections Due to Vancomycin-Resistant Enterococcus at a Hospital in the Era of COVID-19: More than Hand Hygiene Is Needed","volume":"55","author":"Fukushige","year":"2022","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_23","unstructured":"Levitus, M., Rewane, A., and Perera, T.B. (2022). Vancomycin-Resistant Enterococci, StatPearls Publishing."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e897","DOI":"10.1016\/S2468-2667(22)00225-0","article-title":"The Burden of Bacterial Antimicrobial Resistance in the WHO European Region in 2019: A Cross-Country Systematic Analysis","volume":"7","author":"Mestrovic","year":"2022","journal-title":"Lancet Public Health"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1089\/mdr.2021.0109","article-title":"Prevalence of Antibiotic Resistance Over Time in a Third-Level University Hospital","volume":"28","author":"Scaglione","year":"2022","journal-title":"Microb. Drug Resist."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1800516","DOI":"10.2807\/1560-7917.ES.2018.23.46.1800516","article-title":"Prevalence of Healthcare-Associated Infections, Estimated Incidence and Composite Antimicrobial Resistance Index in Acute Care Hospitals and Long-Term Care Facilities: Results from Two European Point Prevalence Surveys, 2016 to 2017","volume":"23","author":"Suetens","year":"2018","journal-title":"Euro Surveill."},{"key":"ref_27","unstructured":"Ecdc (2020). Antimicrobial Resistance in the EU\/EEA (EARS-Net)."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Rangel, K., Chagas, T.P.G., and De-Simone, S.G. (2021). Acinetobacter Baumannii Infections in Times of COVID-19 Pandemic. Pathogens, 10.","DOI":"10.3390\/pathogens10081006"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Stefanini, I., de Renzi, G., Foddai, E., Cordani, E., and Mognetti, B. (2021). Profile of Bacterial Infections in COVID-19 Patients: Antimicrobial Resistance in the Time of SARS-CoV-2. Biology, 10.","DOI":"10.3390\/biology10090822"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1045206","DOI":"10.3389\/fmicb.2022.1045206","article-title":"Carbapenem-Resistant Acinetobacter Baumannii: A Challenge in the Intensive Care Unit","volume":"13","author":"Jiang","year":"2022","journal-title":"Front. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Al Sulayyim, H.J., Ismail, R., Al Hamid, A., and Ghafar, N.A. (2022). Antibiotic Resistance during COVID-19: A Systematic Review. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph191911931"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1016\/j.cmi.2022.12.006","article-title":"Antibiotic Resistance Associated with the COVID-19 Pandemic: A Systematic Review and Meta-Analysis","volume":"29","author":"Langford","year":"2023","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Magnasco, L., Mikulska, M., Giacobbe, D.R., Taramasso, L., Vena, A., Dentone, C., Dettori, S., Tutino, S., Labate, L., and di Pilato, V. (2021). Spread of Carbapenem-Resistant Gram-Negatives and Candida Auris during the COVID-19 Pandemic in Critically Ill Patients: One Step Back in Antimicrobial Stewardship?. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010095"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Serweci\u0144ska, L. (2020). Antimicrobials and Antibiotic-Resistant Bacteria: A Risk to the Environment and to Public Health. Water, 12.","DOI":"10.3390\/w12123313"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Polidori, I., Marullo, L., Ialongo, C., Tomassetti, F., Colombo, R., di Gaudio, F., Calugi, G., Marrone, G., Noce, A., and Bernardini, S. (2022). Characterization of Gut Microbiota Composition in Type 2 Diabetes Patients: A Population-Based Study. Int. J. Environ. Res. Public Health, 19.","DOI":"10.3390\/ijerph192315913"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1186\/s13073-021-00843-9","article-title":"Microbiota Restoration Reduces Antibiotic-Resistant Bacteria Gut Colonization in Patients with Recurrent Clostridioides Difficile Infection from the Open-Label PUNCH CD Study","volume":"13","author":"Langdon","year":"2021","journal-title":"Genome Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1045","DOI":"10.1002\/bies.201500071","article-title":"Antibiotic Use and Abuse: A Threat to Mitochondria and Chloroplasts with Impact on Research, Health, and Environment","volume":"37","author":"Wang","year":"2015","journal-title":"Bioessays"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Rhedin, S., Elfving, K., and Berggren, A. (2021). Novel Biomarkers Differentiating Viral from Bacterial Infection in Febrile Children: Future Perspectives for Management in Clinical Praxis. Children, 8.","DOI":"10.3390\/children8111070"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Nicolai, E., Pieri, M., Gratton, E., Motolese, G., and Bernardini, S. (2021). Bacterial Infection Diagnosis and Antibiotic Prescription in 3 h as an Answer to Antibiotic Resistance: The Case of Urinary Tract Infections. Antibiotics, 10.","DOI":"10.3390\/antibiotics10101168"},{"key":"ref_40","first-page":"495","article-title":"Evaluation of BiesseBioscreen as a new methodology for bacteriuria screening","volume":"37","author":"Nicolai","year":"2014","journal-title":"New Microbiol."},{"key":"ref_41","first-page":"52","article-title":"A rapid, point-of-care antibiotic susceptibility test for urinary tract infections","volume":"69","author":"Toosky","year":"2020","journal-title":"J. Med. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/4\/651\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:02:48Z","timestamp":1760122968000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/4\/651"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3,24]]},"references-count":41,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2023,4]]}},"alternative-id":["antibiotics12040651"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12040651","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,3,24]]}}}