{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:25:01Z","timestamp":1793021101212,"version":"4.5.0"},"reference-count":123,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T00:00:00Z","timestamp":1681430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Postgraduate Program in Microbiology of Universidade Estadual de Londrina"},{"name":"National Council for Scientific and Technological Development\u2014CNPq"},{"name":"Ph.D. scholarship Coordination for the Improvement of Higher Education Personnel (CAPES)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Resistant bacteria may kill more people than COVID-19, so the development of new antibacterials is essential, especially against microbial biofilms that are reservoirs of resistant cells. Silver nanoparticles (bioAgNP), biogenically synthesized using Fusarium oxysporum, combined with oregano derivatives, present a strategic antibacterial mechanism and prevent the emergence of resistance against planktonic microorganisms. Antibiofilm activity of four binary combinations was tested against enteroaggregative Escherichia coli (EAEC) and Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC): oregano essential oil (OEO) plus bioAgNP, carvacrol (Car) plus bioAgNP, thymol (Thy) plus bioAgNP, and Car plus Thy. The antibiofilm effect was accessed using crystal violet, MTT, scanning electron microscopy, and Chromobacterium violaceum anti-quorum-sensing assays. All binary combinations acted against preformed biofilm and prevented its formation; they showed improved antibiofilm activity compared to antimicrobials individually by reducing sessile minimal inhibitory concentration up to 87.5% or further decreasing biofilm metabolic activity and total biomass. Thy plus bioAgNP extensively inhibited the growth of biofilm in polystyrene and glass surfaces, disrupted three-dimensional biofilm structure, and quorum-sensing inhibition may be involved in its antibiofilm activity. For the first time, it is shown that bioAgNP combined with oregano has antibiofilm effect against bacteria for which antimicrobials are urgently needed, such as KPC.<\/jats:p>","DOI":"10.3390\/antibiotics12040756","type":"journal-article","created":{"date-parts":[[2023,4,14]],"date-time":"2023-04-14T05:29:29Z","timestamp":1681450169000},"page":"756","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Antibiofilm Effect of Biogenic Silver Nanoparticles Combined with Oregano Derivatives against Carbapenem-Resistant Klebsiella pneumoniae"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4012-9100","authenticated-orcid":false,"given":"Sara","family":"Scandorieiro","sequence":"first","affiliation":[{"name":"Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"},{"name":"Laboratory of Innovation and Cosmeceutical Technology, Department of Pharmaceutical Sciences, Center of Health Sciences, Hospital Universit\u00e1rio de Londrina, Londrina 86038-350, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franciele Maira M. B.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Dermatological, Infectious and Parasitic Diseases, Faculdade de Medicina de Sao Jose do Rio Preto, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mara C. L.","family":"Nogueira","sequence":"additional","affiliation":[{"name":"Department of Dermatological, Infectious and Parasitic Diseases, Faculdade de Medicina de Sao Jose do Rio Preto, S\u00e3o Jos\u00e9 do Rio Preto 15090-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano A.","family":"Panagio","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Mycology and Oral Microbiology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1556-5146","authenticated-orcid":false,"given":"Admilton G.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Microbial Biotechnology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"},{"name":"Laboratory of Electron Microscopy and Microanalysis, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nelson","family":"Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Institute of Biology, Universidade Estadual de Campinas, Campinas 13083-862, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerson","family":"Nakazato","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7170-2155","authenticated-orcid":false,"given":"Renata K. T.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,4,14]]},"reference":[{"key":"ref_1","unstructured":"(2023, February 21). Food and Drug Administration Generally Recognized as Safe (GRAS), Available online: https:\/\/www.fda.gov\/food\/food-ingredients-packaging\/generally-recognized-safe-gras."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/1477-3155-3-8","article-title":"Mechanistic Aspects of Biosynthesis of Silver Nanoparticles by Several Fusarium Oxysporum Strains","volume":"3","author":"Marcato","year":"2005","journal-title":"J. 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