{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,7]],"date-time":"2026-11-07T10:59:16Z","timestamp":1794049156030,"version":"4.8.0"},"reference-count":38,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,5,26]],"date-time":"2023-05-26T00:00:00Z","timestamp":1685059200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Antimicrobial resistance (AMR) has become a major healthcare concern having a rising incidence, especially in pediatric patients who are more susceptible to infections. The aim of our study was to analyze the bacterial species isolated from patients admitted to our tertiary hospital and their AMR profiles. We conducted a retrospective observational study by examining the bacterial cultures collected from pediatric patients admitted to our hospital over a period of one year. We identified the most common bacterial species from 1445 clinical isolates and their AMR patterns using standard microbiological techniques. Our analysis revealed that the most frequently isolated bacterial species were Escherichia coli (23.73%), Staphylococcus aureus (15.64%), Klebsiella species (12.04%), and Pseudomonas species (9.96%). Additionally, these species exhibited varying levels of resistance to commonly used antibiotics. Notably, we observed high rates of resistance among Gram-negative bacteria, including extended-spectrum beta-lactamase-producing Escherichia coli and Klebsiella species. Among Gram-positive bacteria, we observed a high level of methicillin-resistant Staphylococcus aureus. Our findings highlight the urgent need for effective antibiotic management programs and infection control measures to address the rising incidence of AMR in pediatric hospitals. Further research is needed to identify the mechanisms of resistance in these bacterial species and to develop new strategies for preventing and treating infections caused by antibiotic-resistant bacteria in pediatric patients.<\/jats:p>","DOI":"10.3390\/antibiotics12060966","type":"journal-article","created":{"date-parts":[[2023,5,27]],"date-time":"2023-05-27T16:19:37Z","timestamp":1685204377000},"page":"966","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Bacterial Species and Antibiotic Resistance\u2014A Retrospective Analysis of Bacterial Cultures in a Pediatric Hospital"],"prefix":"10.3390","volume":"12","author":[{"given":"Bianca","family":"Prajescu","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liana","family":"Gavriliu","sequence":"additional","affiliation":[{"name":"Department for Prevention of Healthcare-Associated Infections, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"},{"name":"Department of Infectious Disease, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9766-1558","authenticated-orcid":false,"given":"Mara Ioana","family":"Iesanu","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"},{"name":"Department of Physiology, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8628-3352","authenticated-orcid":false,"given":"Andreea","family":"Ioan","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7305-2192","authenticated-orcid":false,"given":"Anca Andreea","family":"Boboc","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"},{"name":"Department of Pediatrics, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Ro-mania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catalin","family":"Boboc","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felicia","family":"Galos","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Marie Curie Emergency Children\u2019s Hospital, 041451 Bucharest, Romania"},{"name":"Department of Pediatrics, Carol Davila University of Medicine and Pharmacy, 020021 Bucharest, Ro-mania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00048-19","DOI":"10.1128\/CMR.00048-19","article-title":"Antimicrobial Resistance Surveillance in Low- and Middle-Income Countries: Progress and Challenges in Eight South Asian and Southeast Asian Countries","volume":"33","author":"Gandra","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e51034","DOI":"10.15252\/embr.202051034","article-title":"Antibiotic resistance and persistence\u2014Implications for human health and treatment perspectives","volume":"21","author":"Huemer","year":"2020","journal-title":"EMBO Rep."},{"key":"ref_3","first-page":"11","article-title":"Systematic Review of Antibiotic Resistance Rates Among Gram-Negative Bacteria in Children with Sepsis in Resource-Limited Countries","volume":"4","author":"Bielicki","year":"2014","journal-title":"J. Pediatr. Infect. Dis. Soc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.jgar.2020.10.009","article-title":"Antibiotic resistance surveillance systems: A review","volume":"23","author":"Diallo","year":"2020","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"886","DOI":"10.1001\/archinte.1991.00400050040010","article-title":"Mechanisms of bacterial resistance to antibiotics","volume":"151","author":"Dever","year":"1991","journal-title":"Arch. Intern. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e00283-21","DOI":"10.1128\/Spectrum.00283-21","article-title":"Bacterial Epidemiology and Antimicrobial Resistance Profiles in Children Reported by the ISPED Program in China, 2016 to 2020","volume":"9","author":"Fu","year":"2021","journal-title":"Microbiol. Spectr."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"541340","DOI":"10.1155\/2014\/541340","article-title":"Multidrug Resistance: An Emerging Crisis","volume":"2014","author":"Tanwar","year":"2014","journal-title":"Interdiscip. Perspect. Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S128","DOI":"10.1093\/cid\/ciy657","article-title":"Current Status and Trends of Antibacterial Resistance in China","volume":"67","author":"Hu","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"87","DOI":"10.4103\/ijmr.IJMR_214_18","article-title":"Antimicrobial susceptibility profile & resistance mechanisms of Global Antimicrobial Resistance Surveillance System (GLASS) priority pathogens from India","volume":"149","author":"Veeraraghavan","year":"2019","journal-title":"Indian J. Med Res."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Martinez-Vega, R., Jauneikaite, E., Thoon, K.C., Chua, H.Y., Chua, A.H., Khong, W.X., Tan, B.H., Hong, J.L.G., Venkatachalam, I., and Tambyah, P.A. (2019). Risk factor profiles and clinical outcomes for children and adults with pneumococcal infections in Singapore: A need to expand vaccination policy?. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0220951"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1639","DOI":"10.1093\/jac\/dkaa065","article-title":"Progress towards a coordinated, national paediatric antimicrobial resistance surveillance programme: Staphylococcus aureus, enterococcal and Gram-negative bacteraemia in Australia","volume":"75","author":"Campbell","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1645","DOI":"10.2147\/IDR.S173867","article-title":"Antibiotic resistance: A rundown of a global crisis","volume":"11","author":"Aslam","year":"2018","journal-title":"Infect. Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Tsutsui, A., and Suzuki, S. (2018). Japan nosocomial infections surveillance (JANIS): A model of sustainable national antimicrobial resistance surveillance based on hospital diagnostic microbiology laboratories. BMC Heal. Serv. Res., 18.","DOI":"10.1186\/s12913-018-3604-x"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.ijid.2016.08.002","article-title":"Trends in antibiotic resistance among major bacterial pathogens isolated from blood cultures tested at a large private laboratory network in India, 2008\u20132014","volume":"50","author":"Gandra","year":"2016","journal-title":"Int. J. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Romandini, A., Pani, A., Schenardi, P.A., Pattarino, G.A.C., De Giacomo, C., and Scaglione, F. (2021). Antibiotic Resistance in Pediatric Infections: Global Emerging Threats, Predicting the Near Future. Antibiotics, 10.","DOI":"10.3390\/antibiotics10040393"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1097\/QCO.0000000000000371","article-title":"Antimicrobial-resistant Gram-negative infections in neonates","volume":"30","author":"Folgori","year":"2017","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_17","first-page":"675","article-title":"A Cross Sectional Study on Knowledge, Attitude and Practice of Medical Students Toward Antibiotic Resistance and its Prescription, Iran","volume":"8","author":"Jorak","year":"2014","journal-title":"Adv. Environ. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1723","DOI":"10.2147\/IDR.S169176","article-title":"Epidemiology of antibiotic resistance of blood culture in educational hospitals in Rasht, North of Iran","volume":"11","author":"Keihanian","year":"2018","journal-title":"Infect. Drug Resist."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2089","DOI":"10.2147\/IDR.S215329","article-title":"Evaluating the antimicrobial resistance patterns among major bacterial pathogens isolated from clinical specimens taken from patients in Mofid Children\u2019s Hospital, Tehran, Iran: 2013\u20132018","volume":"12","author":"Azimi","year":"2019","journal-title":"Infect. Drug Resist."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1179\/2047773215Y.0000000030","article-title":"Antimicrobial resistance: A global multifaceted phenomenon","volume":"109","author":"Prestinaci","year":"2015","journal-title":"Pathog. Glob. Health"},{"key":"ref_21","unstructured":"(2023, April 14). Re\u021beaua na\u021bional\u0103 de supraveghere a rezisten\u021bei bacteriene\u2014Acas\u0103. Available online: http:\/\/www.carss.cn\/."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Pierantoni, L., Andreozzi, L., Ambretti, S., Dondi, A., Biagi, C., Baccelli, F., and Lanari, M. (2021). Three-Year Trend in Escherichia coli Antimicrobial Resistance among Children\u2019s Urine Cultures in an Italian Metropolitan Area. Children, 8.","DOI":"10.3390\/children8070597"},{"key":"ref_23","first-page":"373","article-title":"Extended-Spectrum Beta-Lactamase Bacteria From Urine Isolates in Children","volume":"20","author":"Degnan","year":"2015","journal-title":"J. Pediatr. Pharmacol. Ther."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1590\/S1413-86702007000200020","article-title":"Risk factors for nosocomial bloodstream infection caused by multidrug resistant gram-negative bacilli in pediatrics","volume":"11","author":"Arnoni","year":"2007","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1128\/AAC.46.5.1481-1491.2002","article-title":"Bloodstream Infections by Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli and Klebsiella pneumoniae in Children: Epidemiology and Clinical Outcome","volume":"46","author":"Kim","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"942","DOI":"10.1542\/peds.2004-1289","article-title":"Risk Factors for and Outcomes of Bloodstream Infection Caused by Extended-Spectrum \u03b2-Lactamase\u2013Producing Escherichia coli and Klebsiella Species in Children","volume":"115","author":"Zaoutis","year":"2005","journal-title":"Pediatrics"},{"key":"ref_27","unstructured":"(2023, April 25). Consumul-de-antibiotice-Rezistenta-Microbiana-si-Infectii-Asociate-Asistentei-Medicale-in-Romania-2020\u2014Institutul Na\u021bional de S\u0103n\u0103tate Public\u0103, 30 September 2022, Available online: https:\/\/insp.gov.ro\/wpfb-file\/consumul-de-antibiotice-rezistenta-microbiana-si-infectii-asociate-asistentei-medicale-in-romania-2020-pdf\/."},{"key":"ref_28","unstructured":"European Centre for Disease Prevention and Control and World Health Organization (2022). Antimicrobial Resistance Surveillance in Europe: 2022: 2020 Data, Publications Office. Available online: https:\/\/data.europa.eu\/doi\/10.2900\/112339."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1186\/s13052-022-01262-1","article-title":"Epidemiology and antimicrobial susceptibility of Staphylococcus aureus in children in a tertiary care pediatric hospital in Milan, Italy, 2017\u20142021","volume":"48","author":"Ippolito","year":"2022","journal-title":"Ital. J. Pediatr."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Crandall, H., Kapusta, A., Killpack, J., Heyrend, C., Nilsson, K., Dickey, M., Daly, J.A., Ampofo, K., Pavia, A.T., and Mulvey, M.A. (2020). Clinical and molecular epidemiology of invasive Staphylococcus aureus infection in Utah children; continued dominance of MSSA over MRSA. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0238991"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1086\/502527","article-title":"Healthcare-Associated Staphylococcus aureus Bacteremia and the Risk for Methicillin Resistance: Is the Centers for Disease Control and Prevention Definition for Community-Acquired Bacteremia Still Appropriate?","volume":"26","author":"Lesens","year":"2005","journal-title":"Infect. Control. Hosp. Epidemiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1111\/imj.13642","article-title":"Healthcare-acquired infections: Prevention strategies","volume":"47","author":"Fernando","year":"2017","journal-title":"Intern. Med. J."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e62","DOI":"10.1093\/jpids\/piw092","article-title":"High Reported Rates of Antimicrobial Resistance in Indian Neonatal and Pediatric Blood Stream Infections","volume":"6","author":"Dharmapalan","year":"2017","journal-title":"J. Pediatr. Infect. Dis. Soc."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"738223","DOI":"10.3389\/fcimb.2021.738223","article-title":"Antibiotic Resistance and Virulence Profiles of Klebsiella pneumoniae Strains Isolated from Different Clinical Sources","volume":"11","author":"Gabasa","year":"2021","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"2475067","DOI":"10.1155\/2016\/2475067","article-title":"Mechanisms of Antimicrobial Resistance in ESKAPE Pathogens","volume":"2016","author":"Santajit","year":"2016","journal-title":"BioMed Res. Int."},{"key":"ref_36","unstructured":"Tacconelli, E. (2023, April 15). Global Priority List of Antibiotic-Resistant Bacteria to Guide Research, Discovery, and Development. Available online: https:\/\/policycommons.net\/artifacts\/1818147\/global-priority-list-of-antibiotic-resistant-bacteria-to-guide-research-discovery-and-development\/2555608\/."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Galo\u0219, F., Boboc, C., Ie\u0219anu, M.-I., Anghel, M., Ioan, A., Iana, E., Co\u0219oreanu, M.T., and Boboc, A.A. (2023). Antibiotic Resistance and Therapeutic Efficacy of Helicobacter pylori Infection in Pediatric Patients\u2014A Tertiary Center Experience. Antibiotics, 12.","DOI":"10.3390\/antibiotics12010146"},{"key":"ref_38","unstructured":"(2023, April 03). ECDC Country Visit to Romania to Discuss Antimicrobial Resistance Issues. Available online: https:\/\/www.ecdc.europa.eu\/en\/publications-data\/ecdc-country-visit-romania-discuss-antimicrobial-resistance-issues."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/6\/966\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:42:49Z","timestamp":1760125369000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/6\/966"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,5,26]]},"references-count":38,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2023,6]]}},"alternative-id":["antibiotics12060966"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12060966","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,5,26]]}}}