{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T12:53:38Z","timestamp":1792155218238,"version":"4.4.0"},"reference-count":49,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2023,6,5]],"date-time":"2023-06-05T00:00:00Z","timestamp":1685923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Carnegie Corporation of New York","award":["RY89"],"award-info":[{"award-number":["RY89"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Beta-lactamase (\u03b2-lactamase)-producing Gram-negative bacteria (GNB) are of public health concern due to their resistance to routine antimicrobials. We investigated the antimicrobial resistance and occurrence of carbapenemases, extended-spectrum \u03b2-lactamases (ESBLs) and AmpCs among GNB from clinical sources. GNB were identified using matrix-assisted laser desorption\/ionization time of flight\u2013mass spectrometry (MALDITOF-MS). Antimicrobial susceptibility testing was performed via Kirby\u2013Bauer disk diffusion and a microscan autoSCAN system. \u03b2-lactamase genes were determined via multiplex polymerase chain reactions. Of the 181 archived GNB analyzed, Escherichia coli and Klebsiella pneumoniae constituted 46% (n = 83) and 17% (n = 30), respectively. Resistance to ampicillin (51%), third-generation cephalosporins (21%), and ertapenem (21%) was observed among the isolates, with 44% being multi-drug resistant (MDR). \u03b2-lactamase genes such as AmpCs ((blaFOX-M (64%) and blaDHA-M and blaEDC-M (27%)), ESBLs ((blaCTX-M (81%), other \u03b2-lactamase genes blaTEM (73%) and blaSHV (27%)) and carbapenemase ((blaOXA-48 (60%) and blaNDM and blaKPC (40%)) were also detected. One K. pneumoniae co-harbored AmpC (blaFOX-M and blaEBC-M) and carbapenemase (blaKPC and blaOXA-48) genes. blaOXA-48 gene was detected in one carbapenem-resistant Acinetobacter baumannii. Overall, isolates were resistant to a wide range of antimicrobials including last-line treatment options. This underpins the need for continuous surveillance for effective management of infections caused by these pathogens in our settings.<\/jats:p>","DOI":"10.3390\/antibiotics12061016","type":"journal-article","created":{"date-parts":[[2023,6,6]],"date-time":"2023-06-06T01:38:26Z","timestamp":1686015506000},"page":"1016","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Occurrence of Carbapenemases, Extended-Spectrum Beta-Lactamases and AmpCs among Beta-Lactamase-Producing Gram-Negative Bacteria from Clinical Sources in Accra, Ghana"],"prefix":"10.3390","volume":"12","author":[{"given":"Felicia A.","family":"Owusu","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noah","family":"Obeng-Nkrumah","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, School of Biomedical and Allied Health Sciences, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Esther","family":"Gyinae","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Korle-Bu Teaching Hospital, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarkodie","family":"Kodom","sequence":"additional","affiliation":[{"name":"University of Ghana Hospital, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhodalyn","family":"Tagoe","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blessing Kofi Adu","family":"Tabi","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4491-6902","authenticated-orcid":false,"given":"Nicholas T. K. D.","family":"Dayie","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Japheth A.","family":"Opintan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University of Ghana Medical School, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beverly","family":"Egyir","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,6,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00181-19","DOI":"10.1128\/CMR.00181-19","article-title":"Antimicrobial Resistance in ESKAPE Pathogens","volume":"33","author":"Forde","year":"2020","journal-title":"Clin. 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