{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T16:29:28Z","timestamp":1787588968040,"version":"build-2736575974"},"reference-count":51,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>A. baumannii imposes a great burden on medical systems worldwide. Surveillance of trends of antibiotic resistance provides a great deal of information needed for antimicrobial stewardship programmes nationwide. Clinical data from long-term, continuous surveillance on trends of antibiotic resistance of A. baumannii in Slovakia is missing. One hundred and forty-nine samples of A. baumannii were isolated over a period of four years. A panel of 19 antibiotics from seven antibiotic categories were tested for the bacterium\u2019s susceptibility. Resistance results were evaluated, and the significance of patterns was estimated using simple linear regression analysis. All isolates were more than 85% resistant to at least 13 out of the 19 tested antibiotics. A significant rise in resistance was recorded for aminoglycosides and imipenem from 2019 to 2022. Colistin and ampicillin-sulbactam have been the only antibiotics maintaining more than 80% efficacy on the bacterium to date. A significant rise in extensively drug-resistant (XDR) strains among carbapenem-resistant (CR) isolates has been recorded. Multidrug-resistance (MDR) among all A. baumannii isolates and XDR among CR strains of the bacterium have risen significantly in the last four years.<\/jats:p>","DOI":"10.3390\/antibiotics12071200","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:47:38Z","timestamp":1689727658000},"page":"1200","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Moving toward Extensively Drug-Resistant: Four-Year Antimicrobial Resistance Trends of Acinetobacter baumannii from the Largest Department of Internal Medicine in Slovakia"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0991-1727","authenticated-orcid":false,"given":"Yashar","family":"Jalali","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Comenius University in Bratislava, 5th Department of Internal Medicine, University Hospital Bratislava, Ru\u017einov, \u0160pit\u00e1lska 24, 813 72, and Ru\u017einovsk\u00e1 4810\/6, 821 01 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1677-0590","authenticated-orcid":false,"given":"Adri\u00e1na","family":"Lipt\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Faculty of Medicine, Comenius University in Bratislava, \u0160pit\u00e1lska 24, 813 72 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Jalali","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Comenius University in Bratislava, 5th Department of Internal Medicine, University Hospital Bratislava, Ru\u017einov, \u0160pit\u00e1lska 24, 813 72, and Ru\u017einovsk\u00e1 4810\/6, 821 01 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juraj","family":"Payer","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Comenius University in Bratislava, 5th Department of Internal Medicine, University Hospital Bratislava, Ru\u017einov, \u0160pit\u00e1lska 24, 813 72, and Ru\u017einovsk\u00e1 4810\/6, 821 01 Bratislava, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ma, C., and McClean, S. (2021). Mapping Global Prevalence of Acinetobacter baumannii and Recent Vaccine Development to Tackle It. Vaccines, 9.","DOI":"10.3390\/vaccines9060570"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"586","DOI":"10.1016\/j.sjbs.2016.02.009","article-title":"Acinetobacter spp. as nosocomial pathogens: Epidemiology and resistance features","volume":"25","author":"Almasaudi","year":"2018","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1086\/500202","article-title":"The Epidemiology and Control of Acinetobacter baumannii in Health Care Facilities","volume":"42","author":"Fournier","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","unstructured":"Guillermo, T.-I. (2022). The Global Antimicrobial Resistance Epidemic, IntechOpen. Chapter 13."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Ko, S.-Y., Kim, N., Park, S.-Y., Kim, S.-Y., Shin, M., and Lee, J.-C. (2023). Acinetobacter baumannii under Acidic Conditions Induces Colistin Resistance through PmrAB Activation and Lipid A Modification. Antibiotics, 12.","DOI":"10.3390\/antibiotics12050813"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.jhin.2019.09.021","article-title":"Genetics, epidemiology, and clinical manifestations of multidrug-resistant Acinetobacter baumannii","volume":"104","author":"Nasr","year":"2020","journal-title":"J. Hosp. Infect."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez-L\u00f3pez, R., Solano-G\u00e1lvez, S.G., Ju\u00e1rez Vignon-Whaley, J.J., Abello Vaamonde, J.A., Padr\u00f3 Alonzo, L.A., Rivera Res\u00e9ndiz, A., Muleiro \u00c1lvarez, M., Vega L\u00f3pez, E.N., Franyuti-Kelly, G., and \u00c1lvarez-Hern\u00e1ndez, D.A. (2020). Acinetobacter baumannii Resistance: A Real Challenge for Clinicians. Antibiotics, 9.","DOI":"10.3390\/antibiotics9040205"},{"key":"ref_8","unstructured":"European Centre for Disease Prevention and Control (2021). Surveillance Atlas of Infectious Diseases."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Novovi\u0107, K., and Jov\u010di\u0107, B. (2023). Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology. Antibiotics, 12.","DOI":"10.3390\/antibiotics12030516"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Arefian, H., Hagel, S., Fischer, D., Scherag, A., Brunkhorst, F.M., Maschmann, J., and Hartmann, M. (2019). Estimating extra length of stay due to healthcare-associated infections before and after implementation of a hospital-wide infection control program. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0217159"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1001\/jamasurg.2018.3213","article-title":"Association of Hospital Length of Stay and Complications With Readmission After Open Pancreaticoduodenectomy","volume":"154","author":"Jiang","year":"2019","journal-title":"JAMA Surg."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"8006","DOI":"10.1038\/s41598-020-65019-8","article-title":"An Extended Duration of the Pre-Operative Hospitalization is Associated with an Increased Risk of Healthcare-Associated Infections after Cardiac Surgery","volume":"10","author":"Sulzgruber","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_13","first-page":"379","article-title":"Isolated carbapenem resistant bacteria, their multidrug resistant profile, percentage of healthcare associated infection and associated mortality, in hospitalized patients in a University Hospital in Bratislava","volume":"122","author":"Jalali","year":"2021","journal-title":"Bratisl. Lek. Listy"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"868","DOI":"10.1177\/003335490912400615","article-title":"Comparison of risk factors for recovery of Acinetobacter baumannii during outbreaks at two Kentucky hospitals, 2006","volume":"124","author":"Beavers","year":"2009","journal-title":"Public Health Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e764","DOI":"10.1016\/j.ijid.2010.02.2254","article-title":"A multicenter study of risk factors and outcome of hospitalized patients with infections due to carbapenem-resistant Acinetobacter baumannii","volume":"14","author":"Sheng","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Alrahmany, D., Omar, A.F., Alreesi, A., Harb, G., and Ghazi, I.M. (2022). Acinetobacter baumannii Infection-Related Mortality in Hospitalized Patients: Risk Factors and Potential Targets for Clinical and Antimicrobial Stewardship Interventions. Antibiotics, 11.","DOI":"10.3390\/antibiotics11081086"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1186\/s13756-015-0083-2","article-title":"Risk factors for hospital-acquired antimicrobial-resistant infection caused by Acinetobacter baumannii","volume":"4","author":"Ellis","year":"2015","journal-title":"Antimicrob. Resist. Infect. Control"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1016\/j.jmii.2019.03.004","article-title":"Clinical characteristics and outcomes of community and hospital-acquired Acinetobacter baumannii bacteremia","volume":"52","author":"Chusri","year":"2019","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"149","DOI":"10.4103\/0972-5229.128705","article-title":"Guidelines for prevention of hospital acquired infections","volume":"18","author":"Mehta","year":"2014","journal-title":"Indian J. Crit. Care Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1086\/517956","article-title":"Importation of Acinetobacter baumannii Into a Burn Unit: A Recurrent Outbreak of Infection Associated With Widespread Environmental Contamination","volume":"28","author":"Zanetti","year":"2007","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e01631-17","DOI":"10.1128\/AAC.01631-17","article-title":"Risk Factors and Outcomes Associated with Multidrug-Resistant Acinetobacter baumannii upon Intensive Care Unit Admission","volume":"62","author":"Blanco","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zeighami, H., Valadkhani, F., Shapouri, R., Samadi, E., and Haghi, F. (2019). Virulence characteristics of multidrug resistant biofilm forming Acinetobacter baumannii isolated from intensive care unit patients. BMC Infect. Dis., 19.","DOI":"10.1186\/s12879-019-4272-0"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.ajic.2021.09.001","article-title":"Isolation of extensively drug resistant Acinetobacter baumannii from environmental surfaces inside intensive care units","volume":"50","author":"Ababneh","year":"2022","journal-title":"Am. J. Infect. Control"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Ceparano, M., Baccolini, V., Migliara, G., Isonne, C., Renzi, E., Tufi, D., De Vito, C., De Giusti, M., Trancassini, M., and Alessandri, F. (2022). Acinetobacter baumannii Isolates from COVID-19 Patients in a Hospital Intensive Care Unit: Molecular Typing and Risk Factors. Microorganisms, 10.","DOI":"10.3390\/microorganisms10040722"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Sirijatuphat, R., Thawornkaew, S., Ruangkriengsin, D., and Thamlikitkul, V. (2022). Colistin Monotherapy versus Colistin plus Sitafloxacin for Therapy of Carbapenem-Resistant Acinetobacter baumannii Infections: A Preliminary Study. Antibiotics, 11.","DOI":"10.3390\/antibiotics11121707"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Kogilathota Jagirdhar, G.S., Rama, K., Reddy, S.T., Pattnaik, H., Qasba, R.K., Elmati, P.R., Kashyap, R., Schito, M., and Gupta, N. (2023). Efficacy of Cefoperazone Sulbactam in Patients with Acinetobacter Infections: A Systematic Review of the Literature. Antibiotics, 12.","DOI":"10.3390\/antibiotics12030582"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Ungthammakhun, C., Vasikasin, V., and Changpradub, D. (2022). A Randomized Controlled Trial of Colistin Combined with Sulbactam: 9 g per Day versus 12 g per Day in the Treatment of Extensively Drug-Resistant Acinetobacter baumannii Pneumonia: An Interim Analysis. Antibiotics, 11.","DOI":"10.3390\/antibiotics11081112"},{"key":"ref_28","unstructured":"European Centre for Disease Prevention and Control (2021). Antimicrobial Consumption in the EU\/EEA (ESAC-Net)\u2014Annual Epidemiological Report for 2021."},{"key":"ref_29","unstructured":"European Centre for Disease Prevention and Control (2022). Antimicrobial resistance in the EU\/EEA (EARS-Net), Yearly."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: The WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3113","DOI":"10.2147\/IDR.S269971","article-title":"Carbapenemase Producers Among Extensive Drug-Resistant Gram-Negative Pathogens Recovered from Febrile Neutrophilic Patients in Egypt","volume":"13","author":"Mabrouk","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1186\/s12348-021-00268-w","article-title":"Virulence factors in multidrug (MDR) and Pan-drug resistant (XDR) Pseudomonas aeruginosa: A cross-sectional study of isolates recovered from ocular infections in a high-incidence setting in southern India","volume":"11","author":"Naik","year":"2021","journal-title":"J. Ophthalmic Inflamm. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e0197521","DOI":"10.1128\/aac.01975-21","article-title":"Treatment, Clinical Outcomes, and Predictors of Mortality among a National Cohort of Admitted Patients with Acinetobacter baumannii Infection","volume":"66","author":"Appaneal","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e00334-19","DOI":"10.1128\/AAC.00334-19","article-title":"Intercellular Transfer of Chromosomal Antimicrobial Resistance Genes between Acinetobacter baumannii Strains Mediated by Prophages","volume":"63","author":"Wachino","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Leungtongkam, U., Thummeepak, R., Tasanapak, K., and Sitthisak, S. (2018). Acquisition and transfer of antibiotic resistance genes in association with conjugative plasmid or class 1 integrons of Acinetobacter baumannii. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0208468"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"702","DOI":"10.1038\/s41467-023-36252-2","article-title":"Cross-protection and cross-feeding between Klebsiella pneumoniae and Acinetobacter baumannii promotes their co-existence","volume":"14","author":"Semenec","year":"2023","journal-title":"Nat. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Vijayakumar, S., John, J.J., Vasudevan, K., Mathur, P., Ray, P., Neeravi, A., Baskaran, A., Kirubananthan, A., Anandan, S., and Biswas, I. (2022). Genomic rearrangements of mobile genetic elements associated with carbapenem resistance of Acinetobacter baumannii. bioRxiv.","DOI":"10.1101\/2022.02.03.478955"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/j.mib.2018.04.004","article-title":"Klebsiella pneumoniae as a key trafficker of drug resistance genes from environmental to clinically important bacteria","volume":"45","author":"Wyres","year":"2018","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_39","first-page":"e000196","article-title":"Genetic diversity, mobilisation and spread of the yersiniabactin-encoding mobile element ICEKp in Klebsiella pneumoniae populations","volume":"4","author":"Lam","year":"2018","journal-title":"Microb. Genom."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Kore\u0148, J., Andrez\u00e1l, M., Drahovsk\u00e1, H., Huben\u00e1kov\u00e1, Z., Lipt\u00e1kov\u00e1, A., and Maliar, T. (2022). Next-Generation Sequencing of Carbapenem-Resistant Klebsiella pneumoniae Strains Isolated from Patients Hospitalized in the University Hospital Facilities. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111538"},{"key":"ref_41","first-page":"935","article-title":"Emergence of extended-spectrum \u03b2-lactamase (ESBL) and\/or carbapenemase producing Enterobacteriaceae (CPE) and their antimicrobial resistance","volume":"120","author":"Koren","year":"2019","journal-title":"Bratisl. Lek. Listy"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1093\/jac\/dkx322","article-title":"High incidence of pandrug-resistant Acinetobacter baumannii isolates collected from patients with ventilator-associated pneumonia in Greece, Italy and Spain as part of the MagicBullet clinical trial","volume":"72","author":"Nowak","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Huang, C., Chen, I., and Tang, T. (2022). Colistin Monotherapy versus Colistin plus Meropenem Combination Therapy for the Treatment of Multidrug-Resistant Acinetobacter baumannii Infection: A Meta-Analysis. J. Clin. Med., 11.","DOI":"10.3390\/jcm11113239"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"348","DOI":"10.3389\/fcimb.2018.00348","article-title":"Synergistic Activity of Niclosamide in Combination With Colistin Against Colistin-Susceptible and Colistin-Resistant Acinetobacter baumannii and Klebsiella pneumoniae","volume":"8","author":"Pachon","year":"2018","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"207","DOI":"10.4103\/ijp.ijp_1013_20","article-title":"Effect of combined colistin and meropenem against meropenem resistant Acinetobacter baumannii and Pseudomonas aeruginosa by checkerboard method: A cross sectional analytical study","volume":"53","author":"Gunalan","year":"2021","journal-title":"Indian J. Pharmacol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Katip, W., Uitrakul, S., and Oberdorfer, P. (2020). A Comparison of Colistin versus Colistin Plus Meropenem for the Treatment of Carbapenem-Resistant Acinetobacter baumannii in Critically Ill Patients: A Propensity Score-Matched Analysis. Antibiotics, 9.","DOI":"10.3390\/antibiotics9100647"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"EVIDoa2200131","DOI":"10.1056\/EVIDoa2200131","article-title":"Colistin Monotherapy versus Combination Therapy for Carbapenem-Resistant Organisms","volume":"2","author":"Kaye","year":"2023","journal-title":"NEJM Evid."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"193","DOI":"10.3389\/fcimb.2019.00193","article-title":"Drug Repurposing to Fight Colistin and Carbapenem-Resistant Bacteria","volume":"9","author":"Peyclit","year":"2019","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1016\/j.drudis.2016.05.015","article-title":"Drug combination therapy increases successful drug repositioning","volume":"21","author":"Sun","year":"2016","journal-title":"Drug Discov. Today"},{"key":"ref_50","unstructured":"Merck (2020, July 20). MALDI-MS Analysis of Proteins. Available online: https:\/\/www.sigmaaldrich.com\/life-science\/proteomics\/recombinant-protein-expression\/protocols\/maldims-analysis-of-proteins.html?gclid=Cj0KCQiAk53-BRD0ARIsAJuNhpvUwYCIMd5NxDbG8_w2Btapg0Mg0Ixf1ueMvjLxZgSP302NkYcR6CQaApLTEALw_wcB."},{"key":"ref_51","unstructured":"EUCAST (2023). Clinical Breakpoints\u2014Breakpoints and Guidance, EUCAST."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/7\/1200\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:14:20Z","timestamp":1760127260000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/7\/1200"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,18]]},"references-count":51,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["antibiotics12071200"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12071200","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,18]]}}}