{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:17:01Z","timestamp":1782915421893,"version":"3.54.5"},"reference-count":70,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T00:00:00Z","timestamp":1689724800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"European Regional Development Fund of the European Union","doi-asserted-by":"publisher","award":["FIS PI17\/00474"],"award-info":[{"award-number":["FIS PI17\/00474"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"European Regional Development Fund of the European Union","doi-asserted-by":"publisher","award":["BP17-08"],"award-info":[{"award-number":["BP17-08"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Severo Ochoa","award":["FIS PI17\/00474"],"award-info":[{"award-number":["FIS PI17\/00474"]}]},{"name":"Severo Ochoa","award":["BP17-08"],"award-info":[{"award-number":["BP17-08"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Salmonella enterica serovar Derby (S. Derby) ranks fifth among nontyphoidal Salmonella serovars causing human infections in the European Union. S. Derby isolates (36) collected between 2006 and 2018 in a Spanish region (Asturias) from human clinical samples (20) as well as from pig carcasses, pork- or pork and beef-derived products, or wild boar (16) were phenotypically characterized with regard to resistance, and 22 (12 derived from humans and 10 from food-related samples) were also subjected to whole genome sequence analysis. The sequenced isolates belonged to ST40, a common S. Derby sequence type, and were positive for SPI-23, a Salmonella pathogenicity island involved in adherence and invasion of the porcine jejune enterocytes. Isolates were either susceptible (30.6%), or resistant to one or more of the 19 antibiotics tested for (69.4%). Resistances to tetracycline [tet(A), tet(B) and tet(C)], streptomycin (aadA2), sulfonamides (sul1), nalidixic acid [gyrA (Asp87 to Asn)] and ampicillin (blaTEM-1-like) were detected, with frequencies ranging from 8.3% to 66.7%, and were higher in clinical than in food-borne isolates. The fosA7.3 gene was present in all sequenced isolates. The most common phenotype was that conferred by the tet(A), aadA2 and sul1 genes, located within identical or closely related variants of Salmonella Genomic Island 1 (SGI1), where mercury resistance genes were also present. Diverse IncI1-I(\u03b1) plasmids belonging to distinct STs provided antibiotic [blaTEM-1, tet(A) and\/or tet(B)] and heavy metal resistance genes (copper and silver), while small pSC101-like plasmids carried tet(C). Regardless of their location, most resistance genes were associated with genetic elements involved in DNA mobility, including a class one integron, multiple insertion sequences and several intact or truncated transposons. By phylogenetic analysis, the isolates were distributed into two distinct clades, both including food-borne and clinical isolates. One of these clades included all SGI1-like positive isolates, which were found in both kinds of samples throughout the entire period of study. Although the frequency of S. Derby in Asturias was very low (0.5% and 3.1% of the total clinical and food isolates of S. enterica recovered along the period of study), it still represents a burden to human health linked to transmission across the food chain. The information generated in the present study can support further epidemiological surveillance aimed to control this zoonotic pathogen.<\/jats:p>","DOI":"10.3390\/antibiotics12071204","type":"journal-article","created":{"date-parts":[[2023,7,19]],"date-time":"2023-07-19T21:20:14Z","timestamp":1689801614000},"page":"1204","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Incidence and Genomic Background of Antibiotic Resistance in Food-Borne and Clinical Isolates of Salmonella enterica Serovar Derby from Spain"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0041-9237","authenticated-orcid":false,"given":"Xenia","family":"V\u00e1zquez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo (UO), 33006 Oviedo, Spain"},{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"},{"name":"Instituto de Productos L\u00e1cteos de Asturias (IPLA), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), 33300 Villaviciosa, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel","family":"Garc\u00eda-Fierro","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo (UO), 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7081-0936","authenticated-orcid":false,"given":"Javier","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Central de Asturias (HUCA), 33011 Oviedo, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red-Enfermedades Respiratorias, 30627 Madrid, Spain"},{"name":"Research & Innovation, Artificial Intelligence and Statistical Department, Pragmatech AI Solutions, 33001 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Bances","sequence":"additional","affiliation":[{"name":"Laboratorio de Salud P\u00fablica, Direcci\u00f3n General de Salud P\u00fablica, Consejer\u00eda de Salud del Principado de Asturias, 33011 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Herrero-Fresno","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6225-6587","authenticated-orcid":false,"given":"John E.","family":"Olsen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosaura","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"},{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de Oviedo (UO), 33006 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7613-3745","authenticated-orcid":false,"given":"V\u00edctor","family":"Ladero","sequence":"additional","affiliation":[{"name":"Instituto de Productos L\u00e1cteos de Asturias (IPLA), Consejo Superior de Investigaciones Cient\u00edficas (CSIC), 33300 Villaviciosa, Spain"},{"name":"Grupo de Microbiolog\u00eda Molecular, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (IAPA), 33011 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5459-8325","authenticated-orcid":false,"given":"Vanesa","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorio de Referencia de Escherichia coli (LREC), Departamento de Microbiolox\u00eda e Parasitolox\u00eda, Facultade de Veterinaria, Campus Terra, Universidade de Santiago de Compostela, 27002 Lugo, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria de Santiago de Compostela, 15706 Santiago de Compostela, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3402-2908","authenticated-orcid":false,"given":"M. Rosario","family":"Rodicio","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, \u00c1rea de Microbiolog\u00eda, Universidad de Oviedo (UO), 33006 Oviedo, Spain"},{"name":"Grupo de Microbiolog\u00eda Traslacional, Instituto de Investigaci\u00f3n Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1086\/650733","article-title":"The global burden of nontyphoidal Salmonella gastroenteritis","volume":"50","author":"Majowicz","year":"2010","journal-title":"Clin. Infect. Dis."},{"key":"ref_2","unstructured":"WHO (2023, June 05). (World Health Organization): Salmonella (Non-Typhoidal)\u2014Fact Sheet. Available online: http:\/\/www.who.int\/mediacentre\/factsheets\/fs139\/en\/."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority, and European Centre for Disease Prevention and Control (2022). The European Union One Health 2021 Zoonoses Report. EFSA J., 20, e07666.","DOI":"10.2903\/j.efsa.2022.7666"},{"key":"ref_4","unstructured":"WHO (2023, June 05). (World Health Organization): Global Action Plan on Antimicrobial Resistance. World Health Organization. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241509763."},{"key":"ref_5","unstructured":"Grimont, P.A.D., and Weill, F.X. (2007). World Health Organization Collaborating Center for Reference and Research on Salmonella, Institut Pasteur. [9th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1128\/CMR.00088-17","article-title":"Mobile genetic elements associated with antimicrobial resistance","volume":"31","author":"Partridge","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/jac\/46.1.7","article-title":"Epidemic Salmonella typhimurium DT 104\u2014A truly international multiresistant clone","volume":"46","author":"Threlfall","year":"2000","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5725","DOI":"10.1128\/JB.183.19.5725-5732.2001","article-title":"Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona","volume":"183","author":"Boyd","year":"2001","journal-title":"J. Bacteriol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.2217\/fmb.10.122","article-title":"Salmonella genomic islands and antibiotic resistance in Salmonella enterica","volume":"5","author":"Hall","year":"2010","journal-title":"Future Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1016\/j.micinf.2005.12.028","article-title":"The genetics of Salmonella genomic island 1","volume":"8","author":"Mulvey","year":"2006","journal-title":"Microbes Infect."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5655","DOI":"10.1128\/AEM.00425-11","article-title":"Antimicrobial resistance and virulence determinants in European Salmonella genomic island 1-positive Salmonella enterica isolates from different origins","volume":"77","author":"Beutlich","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Cummins, M.L., Hamidian, M., and Djordjevic, S.P. (2020). Salmonella genomic island 1 is broadly disseminated within Gammaproteobacteriaceae. Microorganisms, 8.","DOI":"10.3390\/microorganisms8020161"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.ijfoodmicro.2011.08.020","article-title":"Diversity of Salmonella enterica serovar Derby isolated from pig, pork and humans in Germany","volume":"151","author":"Hauser","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1089\/fpd.2013.1537","article-title":"Genetic diversity and antimicrobial resistance profiles of Salmonella enterica serotype Derby isolated from pigs, pork, and humans in France","volume":"10","author":"Kerouanton","year":"2013","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1016\/j.vetmic.2006.08.001","article-title":"Phenotypic and genotypic differentiation of porcine Salmonella enterica subsp. enterica serovar Derby isolates","volume":"118","author":"Michael","year":"2006","journal-title":"Vet. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"694","DOI":"10.3201\/eid1105.041042","article-title":"Salmonella Derby clonal spread from pork","volume":"11","author":"Valdezate","year":"2005","journal-title":"Emerg. Infect. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1016\/j.fm.2017.08.017","article-title":"Evaluation of WGS based approaches for investigating a food-borne outbreak caused by Salmonella enterica serovar Derby in Germany","volume":"71","author":"Simon","year":"2018","journal-title":"Food Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"591929","DOI":"10.3389\/fmicb.2021.591929","article-title":"Salmonella Derby: A comparative genomic analysis of strains from Germany","volume":"12","author":"Hotzel","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Hayward, M.R., Jansen, V., and Woodward, M.J. (2013). Comparative genomics of Salmonella enterica serovars Derby and Mbandaka, two prevalent serovars associated with different livestock species in the UK. BMC Genom., 14.","DOI":"10.1186\/1471-2164-14-365"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Hayward, M.R., Petrovska, L., Jansen, V.A., and Woodward, M.J. (2016). Population structure and associated phenotypes of Salmonella enterica serovars Derby and Mbandaka overlap with host range. BMC Microbiol., 16.","DOI":"10.1186\/s12866-016-0628-4"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Sevellec, Y., Felten, A., Radomski, N., Granier, S.A., Le Hello, S., Petrovska, L., Mistou, M.Y., and Cadel-Six, S. (2019). Genetic diversity of Salmonella Derby from the poultry sector in Europe. Pathogens., 8.","DOI":"10.1128\/MRA.01597-18"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"889","DOI":"10.3389\/fmicb.2020.00889","article-title":"Source attribution study of sporadic Salmonella Derby cases in France","volume":"11","author":"Sevellec","year":"2020","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"891","DOI":"10.3389\/fmicb.2018.00891","article-title":"Polyphyletic nature of Salmonella enterica serotype Derby and lineage-specific host-association revealed by genome-wide analysis","volume":"9","author":"Sevellec","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Hayward, M.R., AbuOun, M., La Ragione, R.M., Tchorzewska, M.A., Cooley, W.A., Everest, D.J., Petrovska, L., Jansen, V.A., and Woodward, M.J. (2014). SPI-23 of S. Derby: Role in adherence and invasion of porcine tissues. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0107857"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"777817","DOI":"10.3389\/fmicb.2021.777817","article-title":"Long-read sequencing reveals evolution and acquisition of antimicrobial resistance and virulence genes in Salmonella enterica","volume":"12","author":"Li","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_26","unstructured":"CLSI (2019). Performance Standards for Antimicrobial Susceptibility Testing, CLSI Supplement M100."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1089\/1076629041310136","article-title":"Characterization and localization of drug resistance determinants in multidrug-resistant, integron-carrying Salmonella enterica serotype Typhimurium strains","volume":"10","author":"Guerra","year":"2004","journal-title":"Microb. Drug Resist."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3018","DOI":"10.1128\/AAC.43.12.3018","article-title":"Genetic characterization of antimicrobial resistance in Canadian isolates of Salmonella serovar Typhimurium DT104","volume":"43","author":"Ng","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.1128\/AAC.47.3.1169-1172.2003","article-title":"A new sulfonamide resistance gene (sul3) in Escherichia coli is widespread in the pig population of Switzerland","volume":"47","author":"Perreten","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6614","DOI":"10.1093\/nar\/gkw569","article-title":"NCBI prokaryotic genome annotation pipeline","volume":"44","author":"Tatusova","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1093\/bioinformatics\/btr039","article-title":"Easyfig: A genome comparison visualizer","volume":"27","author":"Sullivan","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"783","DOI":"10.2307\/2408678","article-title":"Confidence limits on phylogenies: An approach using the bootstrap","volume":"39","author":"Felsenstein","year":"1985","journal-title":"Evolution"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1128\/MMBR.63.3.507-522.1999","article-title":"Transposon Tn21, flagship of the floating genome","volume":"63","author":"Liebert","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Rehman, M.A., Yin, X., Persaud-Lachhman, M.G., and Diarra, M.S. (2017). First detection of a fosfomycin resistance gene, fosA7, in Salmonella enterica serovar Heidelberg isolated from broiler chickens. Antimicrob. Agents Chemother., 61.","DOI":"10.1128\/AAC.00410-17"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"781306","DOI":"10.3389\/fmicb.2021.781306","article-title":"Chromosomally located fosA7 in Salmonella isolates from China","volume":"12","author":"Wang","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1286","DOI":"10.1093\/jac\/dkac061","article-title":"Comparison of the prevalence and molecular characteristics of fosA3 and fosA7 among Salmonella isolates from food animals in China","volume":"77","author":"Wang","year":"2022","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.plasmid.2010.05.005","article-title":"Complete genome sequence of the incompatibility group I1 plasmid R64","volume":"64","author":"Sampei","year":"2010","journal-title":"Plasmid."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/jb.132.2.734-737.1977","article-title":"Revised interpretation of the origin of the pSC101 plasmid","volume":"132","author":"Cohen","year":"1977","journal-title":"J. Bacteriol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"9415","DOI":"10.1093\/nar\/12.24.9415","article-title":"Complete sequence of pSC101","volume":"12","author":"Bernardi","year":"1984","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Nguyen, S.V., Harhay, D.M., Bono, J.L., Smith, T.P., Fields, P.I., Dinsmore, B.A., Santovenia, M., Kelley, C.M., Wang, R., and Bosilevac, J.M. (2016). Complete and closed genome sequences of 10 Salmonella enterica subsp. enterica serovar Anatum isolates from human and bovine sources. Genome Announc., 4.","DOI":"10.1128\/genomeA.00447-16"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority, and European Centre for Disease Prevention and Control (2021). The European Union One Health 2020 Zoonoses Report. EFSA J., 19, e06971.","DOI":"10.2903\/j.efsa.2021.6971"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Ito, R., Mustapha, M.M., Tomich, A.D., Callaghan, J.D., McElheny, C.L., Mettus, R.T., Shanks, R.M.Q., Sluis-Cremer, N., and Doi, Y. (2017). Widespread fosfomycin resistance in Gram-negative bacteria attributable to the chromosomal fosA gene. mBio., 8.","DOI":"10.1128\/mBio.00749-17"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1016\/j.jiac.2016.01.010","article-title":"Fosfomycin: Resurgence of an old companion","volume":"22","author":"Sastry","year":"2016","journal-title":"J. Infect. Chemother."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1123","DOI":"10.1111\/j.1365-2672.2012.05301.x","article-title":"Characterization of a mobile and multiple resistance plasmid isolated from swine manure and its detection in soil after manure application","volume":"112","author":"Rahube","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"653","DOI":"10.1093\/molbev\/msg074","article-title":"Determining the limits of the evolutionary potential of an antibiotic resistance gene","volume":"20","author":"Salipante","year":"2003","journal-title":"Mol. Biol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"6650","DOI":"10.1128\/JB.181.21.6650-6655.1999","article-title":"Activation of the cryptic aac(6\u2032)-Iy aminoglycoside resistance gene of Salmonella by a chromosomal deletion generating a transcriptional fusion","volume":"181","author":"Magnet","year":"1999","journal-title":"J. Bacteriol."},{"key":"ref_49","unstructured":"WHO (2019). Critically Important Antimicrobials for Human Medicine. License: CC BY-NC-SA 30 IGO, WHO."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1128\/AAC.49.2.862-863.2005","article-title":"High-level resistance to fluoroquinolones linked to mutations in gyrA, parC, and parE in Salmonella enterica serovar Schwarzengrund isolates from humans in Taiwan","volume":"49","author":"Baucheron","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.ijfoodmicro.2011.01.037","article-title":"Characterization of the quinolone resistance mechanism in foodborne Salmonella isolates with high nalidixic acid resistance","volume":"146","author":"Kim","year":"2011","journal-title":"Int. J. Food Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"V\u00e1zquez, X., Fern\u00e1ndez, J., Hern\u00e1ez, S., Rodicio, R., and Rodicio, M.R. (2022). Plasmid-mediated quinolone resistance (PMQR) in two clinical strains of Salmonella enterica serovar Corvallis. Microorganisms., 10.","DOI":"10.3390\/microorganisms10030579"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"11482","DOI":"10.1038\/s41598-021-90299-z","article-title":"Genomic analysis and phylogenetic position of the complex IncC plasmid found in the Spanish monophasic clone of Salmonella enterica serovar Typhimurium","volume":"11","author":"Ladero","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1611","DOI":"10.3201\/eid1210.060589","article-title":"Ciprofloxacin-resistant Salmonella Kentucky in travelers","volume":"12","author":"Weill","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.1017\/S095026881700036X","article-title":"Salmonella in the pork production chain and its impact on human health in the European Union","volume":"145","author":"Bonardi","year":"2017","journal-title":"Epidemiol. Infect."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1016\/j.tim.2006.02.006","article-title":"Co-selection of antibiotic and metal resistance","volume":"14","author":"Wright","year":"2006","journal-title":"Trends Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1089\/mdr.2017.0127","article-title":"Antibiotic resistance in Salmonella from retail foods of animal origin and its association with disinfectant and heavy metal resistance","volume":"24","author":"Deng","year":"2018","journal-title":"Microb. Drug Resist."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"S3","DOI":"10.1016\/j.ijfoodmicro.2010.01.021","article-title":"Food-borne diseases\u2014The challenges of 20 years ago still persist while new ones continue to emerge","volume":"139","author":"Newell","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.plasmid.2011.07.003","article-title":"Comparative genomics and phylogeny of the IncI1 plasmids: A common plasmid type among porcine enterotoxigenic Escherichia coli","volume":"66","author":"Johnson","year":"2011","journal-title":"Plasmid"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1093\/jac\/dkx488","article-title":"Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae","volume":"73","author":"Rozwandowicz","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1128\/AEM.02206-08","article-title":"Tetracycline resistome of the organic pig gut","volume":"75","author":"Kazimierczak","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1111\/j.1574-6968.1997.tb12770.x","article-title":"Characterisation of integrons and antibiotic resistance genes in Danish multiresistant Salmonella enterica Typhimurium DT104","volume":"157","author":"Sandvang","year":"1997","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1089\/107662901750152701","article-title":"Variation in clonality and antibiotic-resistance genes among multirresistant Salmonella enterica serotype Typhimurium phage-type U302 (MR U302) from humans, animals and foods","volume":"7","author":"Walker","year":"2001","journal-title":"Microb. Drug Resist."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/S0378-1135(00)00207-8","article-title":"Characterisation of streptomycin resistance determinants in Danish isolates of Salmonella Typhimurium","volume":"75","author":"Madsen","year":"2000","journal-title":"Vet. Microbiol."},{"key":"ref_65","first-page":"19","article-title":"Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable \u03b2-lactamases (TEM, SHV, CARB)","volume":"82","author":"Arlet","year":"1991","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2299","DOI":"10.1128\/AAC.45.8.2299-2303.2001","article-title":"Large drug resistance virulence plasmids of clinical isolates of Salmonella enterica serovar Choleraesuis","volume":"45","author":"Chu","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1006\/mcpr.2001.0363","article-title":"Multiplex PCR for the detection of tetracycline resistant genes","volume":"15","author":"Ng","year":"2001","journal-title":"Mol. Cell Probes."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1128\/jb.145.3.1365-1373.1981","article-title":"Rapid procedure for detection and isolation of large and small plasmids","volume":"145","author":"Kado","year":"1981","journal-title":"J. Bacteriol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1017\/S0022172400063609","article-title":"Characterization of plasmids conferring resistance to gentamicin and apramycin in strains of Salmonella typhimurium phage type 204c isolated in Britain","volume":"97","author":"Threlfall","year":"1986","journal-title":"J Hyg."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1028","DOI":"10.7164\/antibiotics.39.1028","article-title":"Plasmid pVA517C from Escherichia coli V517 is required for the expression of an antibiotic microcin","volume":"39","author":"Aguilar","year":"1986","journal-title":"J. Antibiot."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/7\/1204\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:14:49Z","timestamp":1760127289000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/7\/1204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,19]]},"references-count":70,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,7]]}},"alternative-id":["antibiotics12071204"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12071204","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,19]]}}}