{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T23:52:48Z","timestamp":1793145168043,"version":"4.5.0"},"reference-count":47,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T00:00:00Z","timestamp":1690329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n (MINCIENCIAS, Colombia)","award":["122284468008"],"award-info":[{"award-number":["122284468008"]}],"id":[{"id":"https:\/\/ror.org\/03fd5ne08","id-type":"ROR","asserted-by":"crossref"}]},{"name":"School of Medicine and Health Sciences (ABN122), Universidad del Rosario, Bogot\u00e1, Colombia","award":["122284468008"],"award-info":[{"award-number":["122284468008"]}],"id":[{"id":"https:\/\/ror.org\/0108mwc04","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Candidiasis is an opportunistic infection affecting immunosuppressed and hospitalized patients, with mortality rates approaching 40% in Colombia. The growing pharmacological resistance of Candida species and the emergence of multidrug-resistant Candida\u00a0auris are major public health problems. Therefore, different antimicrobial peptides (AMPs) are being investigated as therapeutic alternatives to control candidiasis effectively and safely. This work aimed to evaluate the in vitro antifungal activity of three synthetic AMPs, PNR20, PNR20-1, and 35409, against ATCC reference strains of Candida\u00a0albicans, Candida\u00a0glabrata, Candida\u00a0parapsilosis, Candida\u00a0krusei, and Candida\u00a0tropicalis, and clinical isolates of C.\u00a0auris. Antifungal susceptibility testing, determined by broth microdilution, showed that the AMPs have antifungal activity against planktonic cells of all Candida species evaluated. In C. auris and C. albicans, the peptides had an effect on biofilm formation and cell viability, as determined by the XTT assay and flow cytometry, respectively. Also, morphological alterations in the membrane and at the intracellular level of these species were induced by the peptides, as observed by transmission electron microscopy. In vitro, the AMPs had no cytotoxicity against L929 murine fibroblasts. Our results showed that the evaluated AMPs are potential therapeutic alternatives against the most important Candida species in Colombia and the world.<\/jats:p>","DOI":"10.3390\/antibiotics12081234","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T01:27:46Z","timestamp":1690421266000},"page":"1234","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["In Vitro Antifungal Activity of Three Synthetic Peptides against Candida auris and Other Candida Species of Medical Importance"],"prefix":"10.3390","volume":"12","author":[{"given":"Richar","family":"Torres","sequence":"first","affiliation":[{"name":"Faculty of Health Sciences, Universidad Colegio Mayor de Cundinamarca, Bogot\u00e1 110311, Colombia"},{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogot\u00e1 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3485-6934","authenticated-orcid":false,"given":"Adriana","family":"Barreto-Santamar\u00eda","sequence":"additional","affiliation":[{"name":"Receptor-Ligand Department Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogot\u00e1 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriela","family":"Ar\u00e9valo-Pinz\u00f3n","sequence":"additional","affiliation":[{"name":"Receptor-Ligand Department Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogot\u00e1 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7547-5172","authenticated-orcid":false,"given":"Carolina","family":"Firacative","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogot\u00e1 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":false,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogot\u00e1 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3029-118X","authenticated-orcid":false,"given":"Patricia","family":"Escand\u00f3n","sequence":"additional","affiliation":[{"name":"Microbiology Group, Instituto Nacional de Salud, Bogot\u00e1 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4751-2500","authenticated-orcid":false,"given":"Manuel Alfonso","family":"Patarroyo","sequence":"additional","affiliation":[{"name":"Molecular Biology and Immunology Department, Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Bogot\u00e1 111321, Colombia"},{"name":"Microbiology Department, Faculty of Medicine, Universidad Nacional de Colombia, Bogot\u00e1 111321, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-7077","authenticated-orcid":false,"given":"Juli\u00e1n E.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, School of Medicine and Health Sciences, Universidad de Rosario, Bogot\u00e1 111221, Colombia"},{"name":"Public Health Research Group, School of Medicine and Health Sciences, Universidad del Rosario, Bogot\u00e1 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e383","DOI":"10.1016\/S1473-3099(17)30316-X","article-title":"The global problem of antifungal resistance: Prevalence, mechanisms, and management","volume":"17","author":"Perlin","year":"2017","journal-title":"Lancet Infect. 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