{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T02:31:22Z","timestamp":1793413882400,"version":"4.5.0"},"reference-count":90,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T00:00:00Z","timestamp":1691539200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017638","name":"Ministry of Health of the Russian Federation","doi-asserted-by":"publisher","award":["056-00050-21-03"],"award-info":[{"award-number":["056-00050-21-03"]}],"id":[{"id":"10.13039\/501100017638","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Acinetobacter species other than A. baumannii are becoming increasingly more important as opportunistic pathogens for humans. The primary aim of this study was to assess the prevalence, species distribution, antimicrobial resistance patterns, and carbapenemase gene content of clinical Acinetobacter non-baumannii (Anb) isolates that were collected as part of a sentinel surveillance program of bacterial infections in hospitalized patients. The secondary aim was to evaluate the performance of MALDI-TOF MS systems for the species-level identification of Anb isolates. Methods: Clinical bacterial isolates were collected from multiple sites across Russia and Kazakhstan in 2016\u20132022. Species identification was performed by means of MALDI-TOF MS, with the Autobio and Bruker systems used in parallel. The PCR detection of the species-specific blaOXA-51-like gene was used as a means of differentiating A. baumannii from Anb species, and the partial sequencing of the rpoB gene was used as a reference method for Anb species identification. The susceptibility of isolates to antibiotics (amikacin, cefepime, ciprofloxacin, colistin, gentamicin, imipenem, meropenem, sulbactam, tigecycline, tobramycin, and trimethoprim\u2013sulfamethoxazole) was determined using the broth microdilution method. The presence of the most common in Acinetobacter-acquired carbapenemase genes (blaOXA-23-like, blaOXA-24\/40-like, blaOXA-58-like, blaNDM, blaIMP, and blaVIM) was assessed using real-time PCR. Results: In total, 234 isolates were identified as belonging to 14 Anb species. These comprised 6.2% of Acinetobacter spp. and 0.7% of all bacterial isolates from the observations. Among the Anb species, the most abundant were A. pittii (42.7%), A. nosocomialis (13.7%), the A. calcoaceticus\/oleivorans group (9.0%), A. bereziniae (7.7%), and A. geminorum (6.0%). Notably, two environmental species, A. oleivorans and A. courvalinii, were found for the first time in the clinical samples of patients with urinary tract infections. The prevalence of resistance to different antibiotics in Anb species varied from &lt;4% (meropenem and colistin) to 11.2% (gentamicin). Most isolates were susceptible to all antibiotics; however, sporadic isolates of A. bereziniae, A. johnsonii, A. nosocomialis, A. oleivorans, A. pittii, and A. ursingii were resistant to carbapenems. A. bereziniae was more frequently resistant to sulbactam, aminoglycosides, trimethoprim\u2013sulfamethoxazole, and tigecycline than the other species. Four (1.7%) isolates of A. bereziniae, A. johnsonii, A. pittii were found to carry carbapenemase genes (blaOXA-58-like and blaNDM, either alone or in combination). The overall accuracy rates of the species-level identification of Anb isolates with the Autobio and Bruker systems were 80.8% and 88.5%, with misidentifications occurring in 5 and 3 species, respectively. Conclusions: This study provides important new insights into the methods of identification, occurrence, species distribution, and antibiotic resistance traits of clinical Anb isolates.<\/jats:p>","DOI":"10.3390\/antibiotics12081301","type":"journal-article","created":{"date-parts":[[2023,8,9]],"date-time":"2023-08-09T10:08:59Z","timestamp":1691575739000},"page":"1301","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Acinetobacter Non-baumannii Species: Occurrence in Infections in Hospitalized Patients, Identification, and Antibiotic Resistance"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2010-7492","authenticated-orcid":false,"given":"Eugene","family":"Sheck","sequence":"first","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2223-193X","authenticated-orcid":false,"given":"Andrey","family":"Romanov","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Shapovalova","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elvira","family":"Shaidullina","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Martinovich","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natali","family":"Ivanchik","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Mikotina","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Skleenova","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Oloviannikov","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya","family":"Azizov","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Vityazeva","sequence":"additional","affiliation":[{"name":"Republican Children\u2019s Hospital, 185000 Petrozavodsk, Republic of Karelia, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9436-8778","authenticated-orcid":false,"given":"Alyona","family":"Lavrinenko","sequence":"additional","affiliation":[{"name":"Shared Resource Laboratory, Karaganda Medical University, 100008 Karaganda, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8728-1113","authenticated-orcid":false,"given":"Roman","family":"Kozlov","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4633-9910","authenticated-orcid":false,"given":"Mikhail","family":"Edelstein","sequence":"additional","affiliation":[{"name":"Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, 214019 Smolensk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"D801","DOI":"10.1093\/nar\/gkab902","article-title":"TYGS and LPSN: A Database Tandem for Fast and Reliable Genome-Based Classification and Nomenclature of Prokaryotes","volume":"50","author":"Carbasse","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.1128\/CMR.00058-16","article-title":"Clinical and Pathophysiological Overview of Acinetobacter Infections: A Century of Challenges","volume":"30","author":"Wong","year":"2017","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1111\/2049-632X.12125","article-title":"Acinetobacter baumannii: Evolution of a Global Pathogen","volume":"71","author":"Antunes","year":"2014","journal-title":"Pathog. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ramirez, M.S., Bonomo, R.A., and Tolmasky, M.E. (2020). Carbapenemases: Transforming Acinetobacter baumannii into a Yet More Dangerous Menace. Biomolecules, 10.","DOI":"10.3390\/biom10050720"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1086\/595011","article-title":"Bad Bugs, No Drugs: No ESKAPE! An Update from the Infectious Diseases Society of America","volume":"48","author":"Boucher","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","unstructured":"World Health Organization (2023, July 17). Prioritization of Pathogens to Guide Discovery, Research and Development of New Antibiotics for Drug-Resistant Bacterial Infections, Including Tuberculosis. Available online: https:\/\/www.who.int\/publications\/i\/item\/WHO-EMP-IAU-2017.12."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"005018","DOI":"10.1099\/ijsem.0.005018","article-title":"Acinetobacter geminorum sp. nov., Isolated from Human Throat Swabs","volume":"71","author":"Wolf","year":"2021","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4105","DOI":"10.1099\/ijsem.0.001318","article-title":"Acinetobacter dijkshoorniae sp. nov., a Member of the Acinetobacter Calcoaceticus-Acinetobacter baumannii Complex Mainly Recovered from Clinical Samples in Different Countries","volume":"66","author":"Cosgaya","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1007\/s12275-011-0315-y","article-title":"Acinetobacter oleivorans sp. nov. Is Capable of Adhering to and Growing on Diesel-Oil","volume":"49","author":"Kang","year":"2011","journal-title":"J. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1099\/ijsem.0.002470","article-title":"Acinetobacter dijkshoorniae Is a Later Heterotypic Synonym of Acinetobacter Lactucae","volume":"68","author":"Dunlap","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"393","DOI":"10.1016\/j.resmic.2011.02.006","article-title":"Genotypic and Phenotypic Characterization of the Acinetobacter calcoaceticus\u2013Acinetobacter baumannii Complex with the Proposal of Acinetobacter pittii sp. nov. (Formerly Acinetobacter Genomic Species 3) and Acinetobacter nosocomialis sp. nov. (Formerly Acinetobacter Genomic Species 13TU)","volume":"162","author":"Nemec","year":"2011","journal-title":"Res. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Glover, J.S., Ticer, T.D., and Engevik, M.A. (2022). Profiling Antibiotic Resistance in  Acinetobacter calcoaceticus. Antibiotics, 11.","DOI":"10.3390\/antibiotics11070978"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104698","DOI":"10.1016\/j.micpath.2020.104698","article-title":"Updating the Repertoire of Cultured Bacteria from the Human Being","volume":"150","author":"Diakite","year":"2021","journal-title":"Microb. Pathog."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"134","DOI":"10.3201\/eid2201.151298","article-title":"Severe Community-Acquired Bloodstream Infection with Acinetobacter ursingii in Person Who Injects Drugs","volume":"22","author":"Salzer","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4172","DOI":"10.1128\/AAC.02992-14","article-title":"Clinical Outcomes of Hospital-Acquired Infection with Acinetobacter nosocomialis and Acinetobacter pittii","volume":"58","author":"Chusri","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1445","DOI":"10.1128\/JCM.02467-09","article-title":"Incidence of Acinetobacter Species Other than A. baumannii among Clinical Isolates of Acinetobacter: Evidence for Emerging Species","volume":"48","author":"Turton","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"184","DOI":"10.2174\/1871526515666150826114745","article-title":"Acinetobacter lwoffii an Emerging Pathogen in Neonatal ICU","volume":"15","author":"Mittal","year":"2015","journal-title":"Infect. Disord. Drug Targets"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.jinf.2014.09.004","article-title":"Influence of ACB Complex Genospecies on Clinical Outcomes in a U.S. Hospital with High Rates of Multidrug Resistance","volume":"70","author":"Fitzpatrick","year":"2015","journal-title":"J. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1099\/jmm.0.069138-0","article-title":"Distribution of Carbapenem Resistance Determinants among Epidemic and Non-Epidemic Types of Acinetobacter Species in Japan","volume":"63","author":"Matsui","year":"2014","journal-title":"J. Med. Microbiol."},{"key":"ref_20","first-page":"49","article-title":"Reservoirs of Non-baumannii Acinetobacter Species","volume":"7","author":"Hamze","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"FSO395","DOI":"10.2144\/fsoa-2018-0127","article-title":"Accurate Identification of Clinically Important Acinetobacter spp.: An Update","volume":"5","author":"Vijayakumar","year":"2019","journal-title":"Future Sci. OA"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1128\/JCM.44.3.827-832.2006","article-title":"Sequencing of the RpoB Gene and Flanking Spacers for Molecular Identification of Acinetobacter Species","volume":"44","author":"Gundi","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1016\/j.diagmicrobio.2013.07.013","article-title":"Comparison of RpoB Gene Sequencing, 16S RRNA Gene Sequencing, GyrB Multiplex PCR, and the VITEK2 System for Identification of Acinetobacter Clinical Isolates","volume":"78","author":"Lee","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1128\/JCM.01021-06","article-title":"Identification of Acinetobacter baumannii by Detection of the BlaOXA-51-like Carbapenemase Gene Intrinsic to This Species","volume":"44","author":"Turton","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/prca.201500086","article-title":"Recent Development of Mass Spectrometry and Proteomics Applications in Identification and Typing of Bacteria","volume":"10","author":"Cheng","year":"2016","journal-title":"Proteomics Clin. Appl."},{"key":"ref_26","first-page":"e1","article-title":"MALDI-TOF\/MS Identification of Species from the Acinetobacter baumannii (Ab) Group Revisited: Inclusion of the Novel A. seifertii and A. dijkshoorniae Species","volume":"23","author":"Cosgaya","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1546","DOI":"10.1007\/s13361-018-1911-4","article-title":"Insight into Identification of Acinetobacter Species by Matrix-Assisted Laser Desorption\/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) in the Clinical Laboratory","volume":"29","author":"Li","year":"2018","journal-title":"J. Am. Soc. Mass. Spectrom."},{"key":"ref_28","first-page":"e000306","article-title":"Emergence, Molecular Mechanisms and Global Spread of Carbapenem-Resistant Acinetobacter baumannii","volume":"5","author":"Hamidian","year":"2019","journal-title":"Microb. Genom."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Li, P., Yang, C., Xie, J., Liu, N., Wang, H., Zhang, L., Wang, X., Wang, Y., Qiu, S., and Song, H. (2015). Acinetobacter calcoaceticus from a Fatal Case of Pneumonia Harboring BlaNDM-1 on a Widely Distributed Plasmid. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-0870-7"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1538","DOI":"10.1099\/jmm.0.000844","article-title":"Prevalence and Antimicrobial Susceptibilities of Acinetobacter baumannii and Non-baumannii Acinetobacters from Terengganu, Malaysia and Their Carriage of Carbapenemase Genes","volume":"67","author":"Rani","year":"2018","journal-title":"J. Med. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"520","DOI":"10.1016\/j.ijantimicag.2008.10.009","article-title":"Wide Dissemination of OXA-Type Carbapenemases in Clinical Acinetobacter spp. Isolates from South Korea","volume":"33","author":"Lee","year":"2009","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1093\/jac\/dkz080","article-title":"Identification of the Novel Class D \u03b2-Lactamase OXA-679 Involved in Carbapenem Resistance in Acinetobacter calcoaceticus","volume":"74","author":"Tietgen","year":"2019","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e02734-21","DOI":"10.1128\/spectrum.02734-21","article-title":"Carbapenem Resistance in Acinetobacter nosocomialis and Acinetobacter junii Conferred by Acquisition of BlaOXA-24\/40 and Genetic Characterization of the Transmission Mechanism between Acinetobacter Genomic Species","volume":"10","author":"Bou","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1667","DOI":"10.1099\/jmm.0.000845","article-title":"Mechanisms of Carbapenem Resistance in Acinetobacter pittii and Acinetobacter nosocomialis Isolates from Thailand","volume":"67","author":"Chatsuwan","year":"2018","journal-title":"J. Med. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"e12365","DOI":"10.1016\/j.heliyon.2022.e12365","article-title":"First Identification of Multidrug-Resistant Acinetobacter bereziniae Isolates Harboring BlaNDM-1 from Hospitals in South China","volume":"9","author":"Mo","year":"2023","journal-title":"Heliyon"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1399","DOI":"10.1089\/mdr.2019.0210","article-title":"Genomic and Clinical Characterization of IMP-1-Producing Multidrug-Resistant Acinetobacter bereziniae Isolates from Bloodstream Infections in a Brazilian Tertiary Hospital","volume":"26","author":"Tavares","year":"2020","journal-title":"Microb. Drug Resist."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2589","DOI":"10.1128\/AAC.01957-15","article-title":"Old Clinical Isolates of Acinetobacter seifertii in Brazil Producing OXA-58","volume":"60","author":"Matos","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1016\/j.nmni.2017.10.004","article-title":"First Case of Bacteraemia Due to Acinetobacter schindleri Harbouring BlaNDM-1 in an Immunocompromised Patient","volume":"21","author":"Palombarani","year":"2018","journal-title":"New Microbes New Infect."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"628","DOI":"10.1099\/jmm.0.000732","article-title":"Occurrence of IMP-1 in Non-baumannii Acinetobacter Clinical Isolates from Brazil","volume":"67","author":"Streling","year":"2018","journal-title":"J. Med. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.ijantimicag.2011.08.006","article-title":"Characteristics of Carbapenem-Resistant Acinetobacter spp. Other than Acinetobacter baumannii in South Korea","volume":"39","author":"Park","year":"2012","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"02502-19","DOI":"10.1128\/AAC.02502-19","article-title":"Co-Occurrence of Plasmid-Mediated Tigecycline and Carbapenem Resistance in Acinetobacter spp. from Waterfowls and Their Neighboring Environment","volume":"64","author":"Cui","year":"2020","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1111\/1348-0421.12601","article-title":"Species Distribution, Virulence Factors, and Antimicrobial Resistance of Acinetobacter spp. Isolates from Dogs and Cats: A Preliminary Study","volume":"62","author":"Kimura","year":"2018","journal-title":"Microbiol. Immunol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Baraka, A., Traglia, G.M., Monta\u00f1a, S., Tolmasky, M.E., and Ramirez, M.S. (2021). An Acinetobacter Non-baumannii Population Study: Antimicrobial Resistance Genes (ARGs). Antibiotics, 10.","DOI":"10.3390\/antibiotics10010016"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1099\/ijsem.0.000932","article-title":"Taxonomy of Haemolytic and\/or Proteolytic Strains of the Genus Acinetobacter with the Proposal of Acinetobacter courvalinii sp. nov. (Genomic Species 14 Sensu Bouvet & Jeanjean), Acinetobacter dispersus sp. nov. (Genomic Species 17), Acinetobacter modestus sp. nov., Acinetobacter proteolyticus sp. nov. and Acinetobacter vivianii sp. nov","volume":"66","author":"Nemec","year":"2016","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"104287","DOI":"10.1016\/j.micpath.2020.104287","article-title":"Genotypic, Phenotypic, and Pathogenic Characterization of the Soil Isolated Acinetobacter courvalinii","volume":"149","author":"Dey","year":"2020","journal-title":"Microb. Pathog."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1252","DOI":"10.1128\/AAC.01304-07","article-title":"Acinetobacter Radioresistens as a Silent Source of Carbapenem Resistance for Acinetobacter spp.","volume":"52","author":"Poirel","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"620002","DOI":"10.3389\/fmicb.2021.620002","article-title":"AMRmap: An Interactive Web Platform for Analysis of Antimicrobial Resistance Surveillance Data in Russia","volume":"12","author":"Kuzmenkov","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"e01430-14","DOI":"10.1128\/genomeA.01430-14","article-title":"Draft Genome Sequence of Acinetobacter Oleivorans PF1, a Diesel-Degrading and Plant-Growth-Promoting Endophytic Strain Isolated from Poplar Trees Growing on a Diesel-Contaminated Plume","volume":"3","author":"Gkorezis","year":"2015","journal-title":"Genome Announc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1007\/s00284-020-02320-9","article-title":"Characterization of the Biodiesel Degrading Acinetobacter oleivorans Strain PT8 Isolated from the Fecal Material of a Painted Turtle (Chrysemys picta)","volume":"78","author":"Deems","year":"2021","journal-title":"Curr. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"138732","DOI":"10.1016\/j.chemosphere.2023.138732","article-title":"Characterization and Genome Analysis of Acinetobacter oleivorans S4 as an Efficient Hydrocarbon-Degrading and Plant-Growth-Promoting rhizobacterium","volume":"331","author":"Wang","year":"2023","journal-title":"Chemosphere"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"6667623","DOI":"10.1155\/2021\/6667623","article-title":"Comparison of Autof Ms1000 and Bruker Biotyper MALDI-TOF MS Platforms for Routine Identification of Clinical Microorganisms","volume":"2021","author":"Park","year":"2021","journal-title":"Biomed. Res. Int."},{"key":"ref_52","first-page":"280","article-title":"In Vitro Activity of Biapenem and Other Carbapenems against Russian Clinical Isolates of Pseudomonas aeruginosa, Acinetobacter spp., and Enterobacterales","volume":"23","author":"Kozlov","year":"2021","journal-title":"Klin. Mikrobiol. Antimikrobn. Himioter."},{"key":"ref_53","first-page":"171","article-title":"Antimicrobial Resistance, Carbapenemase Production, and Genotypes of Nosocomial Acinetobacter spp. Isolates in Russia: Results of Multicenter Epidemiological Study \u201cMARATHON 2015\u20132016\u201d","volume":"21","author":"Shek","year":"2019","journal-title":"Klin. Mikrobiol. Antimikrobn. Himioter."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"4762","DOI":"10.1128\/AAC.00084-14","article-title":"Resistance to Colistin Associated with a Single Amino Acid Change in Protein PmrB among Klebsiella pneumoniae Isolates of Worldwide Origin","volume":"58","author":"Jayol","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Brovedan, M., Repizo, G.D., Marchiaro, P., Viale, A.M., and Limansky, A. (2019). Characterization of the Diverse Plasmid Pool Harbored by the BlaNDM-1-Containing Acinetobacter bereziniae HPC229 Clinical Strain. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0220584"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"00117-16","DOI":"10.1128\/genomeA.00117-16","article-title":"Draft Genome Sequence of Acinetobacter bereziniae HPC229, a Carbapenem-Resistant Clinical Strain from Argentina Harboring BlaNDM-1","volume":"4","author":"Brovedan","year":"2016","journal-title":"Genome Announc."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1093\/jac\/dkt480","article-title":"Multiplex PCR to Detect the Genes Encoding Naturally Occurring Oxacillinases in Acinetobacter spp.","volume":"69","author":"Kamolvit","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4885","DOI":"10.1128\/JCM.43.9.4885-4888.2005","article-title":"Regional Occurrence of Plasmid-Mediated Carbapenem-Hydrolyzing Oxacillinase OXA-58 in Acinetobacter spp. in Europe","volume":"43","author":"Poirel","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1128\/AAC.50.2.756-758.2006","article-title":"Occurrence of OXA-58-like Carbapenemases in Acinetobacter spp. Collected over 10 Years in Three Continents","volume":"50","author":"Coelho","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1128\/AAC.00999-08","article-title":"Codetection of BlaOXA-23-like Gene (BlaOXA-133) and BlaOXA-58 in Acinetobacter Radioresistens: Report from the SENTRY Antimicrobial Surveillance Program","volume":"53","author":"Mendes","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1128\/AAC.50.1.399-400.2006","article-title":"OXA-58 and IMP-4 Carbapenem-Hydrolyzing Beta-Lactamases in an Acinetobacter junii Blood Culture Isolate from Australia","volume":"50","author":"Peleg","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"2699","DOI":"10.1128\/AAC.01624-09","article-title":"Molecular Characterization of Beta-Lactamase Genes and Their Genetic Structures in Acinetobacter Genospecies 3 Isolates in Taiwan","volume":"54","author":"Huang","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/jac\/dkv324","article-title":"Characterization of Acinetobacter johnsonii Isolate XBB1 Carrying Nine Plasmids and Encoding NDM-1, OXA-58 and PER-1 by Genome Sequencing","volume":"71","author":"Feng","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1016\/j.jgar.2018.11.015","article-title":"Whole-Genome Sequencing of an NDM-1- and OXA-58-Producing Acinetobacter towneri Isolate from Hospital Sewage in Sichuan Province, China","volume":"16","author":"Jiang","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e02633-18","DOI":"10.1128\/AAC.02633-18","article-title":"Emergence of IMP-34- and OXA-58-Producing Carbapenem-Resistant Acinetobacter colistiniresistens","volume":"63","author":"Suzuki","year":"2019","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.jgar.2022.09.006","article-title":"Comparative Analysis of IMP-4- and OXA-58-Containing Plasmids of Three Carbapenemase-Producing Acinetobacter ursingii Strains in the Netherlands","volume":"31","author":"Hendrickx","year":"2022","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.jgar.2019.08.011","article-title":"Detection of OXA-58-Producing Acinetobacter bereziniae in Brazil","volume":"19","author":"Romanin","year":"2019","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.ijantimicag.2015.11.008","article-title":"Emergence of ST119 Acinetobacter pittii Co-Harbouring NDM-1 and OXA-58 in Malaysia","volume":"47","author":"Ang","year":"2016","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.jgar.2018.04.006","article-title":"First Detection of an OXA-58 Carbapenemase-Producing Acinetobacter nosocomialis Clinical Isolate in the Balkan States","volume":"13","author":"Strateva","year":"2018","journal-title":"J. Glob. Antimicrob. Resist."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1086\/504356","article-title":"Dissemination of IMP-1 Metallo- Beta -Lactamase-Producing Acinetobacter Species in a Brazilian Teaching Hospital","volume":"27","author":"Tognim","year":"2006","journal-title":"Infect. Control Hosp. Epidemiol."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1016\/j.ijantimicag.2010.05.018","article-title":"Characteristics of Clinical Isolates of Acinetobacter Genomospecies 10 Carrying Two Different Metallo-Beta-Lactamases","volume":"36","author":"Lee","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1128\/AAC.06199-11","article-title":"Novel Plasmid and Its Variant Harboring Both a Bla(NDM-1) Gene and Type IV Secretion System in Clinical Isolates of Acinetobacter lwoffii","volume":"56","author":"Hu","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_73","doi-asserted-by":"crossref","unstructured":"Wang, Y., Wu, C., Zhang, Q., Qi, J., Liu, H., Wang, Y., He, T., Ma, L., Lai, J., and Shen, Z. (2012). Identification of New Delhi Metallo-\u03b2-Lactamase 1 in Acinetobacter lwoffii of Food Animal Origin. PLoS ONE, 7.","DOI":"10.1371\/annotation\/e33066ea-f500-4d34-8981-7cfd68497e73"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1111\/1469-0691.12013","article-title":"Regional Dissemination of Acinetobacter Species Harbouring Metallo-\u03b2-Lactamase Genes in Japan","volume":"19","author":"Yamamoto","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"e01076-20","DOI":"10.1128\/mSphere.01076-20","article-title":"Complete Genome Sequencing of Acinetobacter baumannii AC1633 and Acinetobacter nosocomialis AC1530 Unveils a Large Multidrug-Resistant Plasmid Encoding the NDM-1 and OXA-58 Carbapenemases","volume":"6","author":"Alattraqchi","year":"2021","journal-title":"mSphere"},{"key":"ref_76","first-page":"106","article-title":"Detection of Co-Harboring OXA-58 and NDM-1 Carbapenemase Producing Genes Resided on a Same Plasmid from an Acinetobacter pittii Clinical Isolate in China","volume":"22","author":"Chen","year":"2019","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1007\/s13205-018-1310-3","article-title":"Molecular Characterization of Carbapenemases of Clinical Acinetobacter baumannii-calcoaceticus Complex Isolates from a University Hospital in Tunisia","volume":"8","author":"Cheikh","year":"2018","journal-title":"3 Biotech."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"8976","DOI":"10.1038\/srep08976","article-title":"\u201cRoar\u201d of BlaNDM-1 and \u201cSilence\u201d of BlaOXA-58 Co-Exist in Acinetobacter pittii","volume":"5","author":"Zhou","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"e4696","DOI":"10.1002\/jms.4696","article-title":"Evaluation of Three Sample Preparation Methods for the Identification of Clinical Strains by Using Two MALDI-TOF MS Systems","volume":"56","author":"Wang","year":"2021","journal-title":"J. Mass Spectrom."},{"key":"ref_80","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2017). Methods for the Identification of Cultured Microorganisms Using Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight Mass Spectrometry, CLSI. [1st ed.]. CLSI supplement M58."},{"key":"ref_81","doi-asserted-by":"crossref","unstructured":"Diancourt, L., Passet, V., Nemec, A., Dijkshoorn, L., and Brisse, S. (2010). The Population Structure of Acinetobacter baumannii: Expanding Multiresistant Clones from an Ancestral Susceptible Genetic Pool. PLoS ONE, 5.","DOI":"10.1371\/journal.pone.0010034"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1128\/JCM.43.9.4382-4390.2005","article-title":"Development of a Multilocus Sequence Typing Scheme for Characterization of Clinical Isolates of Acinetobacter baumannii","volume":"43","author":"Bartual","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"2333","DOI":"10.1099\/mic.0.026054-0","article-title":"Validation of Partial RpoB Gene Sequence Analysis for the Identification of Clinically Important and Emerging Acinetobacter Species","volume":"155","author":"Gundi","year":"2009","journal-title":"Microbiology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"W293","DOI":"10.1093\/nar\/gkab301","article-title":"Interactive Tree Of Life (ITOL) v5: An Online Tool for Phylogenetic Tree Display and Annotation","volume":"49","author":"Letunic","year":"2021","journal-title":"Nucleic Acids Res."},{"key":"ref_86","unstructured":"(2019). Susceptibility Testing of Infectious Agents and Evaluation of Performance of Antimicrobial Susceptibility Test Devices\u2014Part 1: Broth Micro-Dilution Reference Method for Testing the In Vitro Activity of Antimicrobial Agents against Rapidly Growing Aerobic Bacteria Involved in Infectious Diseases (Standard No. ISO 20776-1:2019)."},{"key":"ref_87","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2023, July 17). EUCAST Reading Guide for Broth Microdilution v. 4.0. Available online: https:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Disk_test_documents\/2022_manuals\/Reading_guide_BMD_v_4.0_2022.pdf."},{"key":"ref_88","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (2023, July 17). Breakpoint Tables for Interpretation of MICs and Zone Diameters. Version 13.1. Available online: http:\/\/www.eucast.org."},{"key":"ref_89","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2023). Performance Standards for Antimicrobial Susceptibility Testing, CLSI. [33rd ed.]. CLSI supplement M100."},{"key":"ref_90","first-page":"e000093","article-title":"Microreact: Visualizing and Sharing Data for Genomic Epidemiology and Phylogeography","volume":"2","author":"Abudahab","year":"2016","journal-title":"Microb. Genom."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/8\/1301\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:28:46Z","timestamp":1760128126000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/8\/1301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,9]]},"references-count":90,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["antibiotics12081301"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12081301","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,9]]}}}