{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:30:22Z","timestamp":1760149822340,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T00:00:00Z","timestamp":1694563200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008061","name":"Fundaci\u00f3n Mutua Madrile\u00f1a","doi-asserted-by":"publisher","award":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"],"award-info":[{"award-number":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"]}],"id":[{"id":"10.13039\/100008061","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"ISCIII and the European Regional Development Fund (FEDER)","doi-asserted-by":"publisher","award":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"],"award-info":[{"award-number":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"Health Research Fund (FIS) of the Carlos III Health Institute (ISCIII)","doi-asserted-by":"publisher","award":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"],"award-info":[{"award-number":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004587","name":"ISCIII","doi-asserted-by":"publisher","award":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"],"award-info":[{"award-number":["FMM21\/01","PI21\/00344","ISCIII-MICINN","MSII18\/00008","FI22\/00022"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Based on previous studies by our group in which we demonstrated that dalbavancin loaded in bone cement had good elution capacity for the treatment of biofilm-related periprosthetic infections, we now assess the anti-biofilm activity of dalbavancin and compare it with that of vancomycin over a 3-month period. We designed an in vitro model in which we calculated the percentage reduction in log cfu\/mL counts of sonicated steel discs contaminated with staphylococci and further exposed to bone cement discs loaded with 2.5% or 5% vancomycin and dalbavancin at various timepoints (24 h, 48 h, 1 week, 2 weeks, 6 weeks, and 3 months). In addition, we tested the anti-biofilm activity of eluted vancomycin and dalbavancin at each timepoint based on a 96-well plate model in which we assessed the percentage reduction in metabolic activity. We observed a significant decrease in the dalbavancin concentration from 2 weeks of incubation, with sustained anti-biofilm activity up to 3 months. In the case of vancomycin, we observed a significant decrease at 1 week. The concentration gradually increased, leading to significantly lower anti-biofilm activity. The percentage reduction in cfu\/mL counts was higher for dalbavancin than for vancomycin at both the 2.5% and the 5% concentrations. The reduction in log cfu\/mL counts was higher for S. epidermidis than for S. aureus and was particularly more notable for 5% dalbavancin at 3 months. In addition, the percentage reduction in metabolic activity also decreased at 3 months in 5% dalbavancin and 5% vancomycin, with more notable values recorded for the latter.<\/jats:p>","DOI":"10.3390\/antibiotics12091445","type":"journal-article","created":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T09:57:56Z","timestamp":1694685476000},"page":"1445","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Efficacy of Dalbavancin as a Long-Acting Anti-Biofilm Agent Loaded in Bone Cement"],"prefix":"10.3390","volume":"12","author":[{"given":"Mar","family":"S\u00e1nchez-Somolinos","sequence":"first","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"D\u00edaz-Navarro","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Benjumea","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Traumatology, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Matas","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery and Traumatology, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8785-2278","authenticated-orcid":false,"given":"Javier","family":"Vaquero","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"Department of Orthopaedic Surgery and Traumatology, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"School of Medicine, Traumatology Department, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5706-5583","authenticated-orcid":false,"given":"Patricia","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"Department of Orthopaedic Surgery and Traumatology, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"School of Medicine, Traumatology Department, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"School of Medicine, Microbiology Department, Universidad Complutense de Madrid, 28040 Madrid, Spain"},{"name":"CIBER Enfermedades Respiratorias-CIBERES (CB06\/06\/0058), 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7588-1880","authenticated-orcid":false,"given":"Pablo","family":"Sanz-Ru\u00edz","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"Department of Orthopaedic Surgery and Traumatology, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"School of Medicine, Traumatology Department, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7607-5848","authenticated-orcid":false,"given":"Mar\u00eda","family":"Guembe","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"},{"name":"Instituto de Investigaci\u00f3n Sanitaria Gregorio Mara\u00f1\u00f3n, 28007 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2623","DOI":"10.1007\/s11999-017-5289-6","article-title":"Increased Mortality after Prosthetic Joint Infection in Primary THA","volume":"475","author":"Gundtoft","year":"2017","journal-title":"Clin. Orthop. Relat. Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2203","DOI":"10.1097\/CORR.0000000000001838","article-title":"What Are the Long-term Outcomes of Mortality, Quality of Life, and Hip Function after Prosthetic Joint Infection of the Hip? A 10-year Follow-up from Sweden","volume":"479","author":"Wildeman","year":"2021","journal-title":"Clin. Orthop. Relat. 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Transl."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1302\/2058-5241.4.180095","article-title":"Implant materials and prosthetic joint infection: The battle with the biofilm","volume":"4","author":"Davidson","year":"2019","journal-title":"EFORT Open Rev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1007\/s43465-022-00782-6","article-title":"Biofilms-What Should the Orthopedic Surgeon know?","volume":"57","author":"Dhillon","year":"2023","journal-title":"Indian. J. Orthop."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1302\/2633-1462.44.BJO-2022-0155.R1","article-title":"Development of evidence-based guidelines for the treatment and management of periprosthetic hip infection","volume":"4","author":"Moore","year":"2023","journal-title":"Bone Jt. Open"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1007\/s12306-021-00734-7","article-title":"Management of prosthetic joint infections: A guidelines comparison","volume":"106","author":"Ometti","year":"2022","journal-title":"Musculoskelet. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"i29","DOI":"10.1093\/jac\/dku253","article-title":"Clinical guidelines in the management of prosthetic joint infection","volume":"69","author":"Minassian","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/cis803","article-title":"Diagnosis and management of prosthetic joint infection: Clinical practice guidelines by the Infectious Diseases Society of America","volume":"56","author":"Osmon","year":"2013","journal-title":"Clin Infect Dis."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Steadman, W., Chapman, P.R., Schuetz, M., Schmutz, B., Trampuz, A., and Tetsworth, K. (2023). Local Antibiotic Delivery Options in Prosthetic Joint Infection. Antibiotics, 12.","DOI":"10.3390\/antibiotics12040752"},{"key":"ref_12","first-page":"246","article-title":"Optimal timing for reimplantation in the setting of two stage revision for prosthetic joint infection","volume":"67","author":"Fraval","year":"2023","journal-title":"Rev. Esp. De Cir. Ortop. Y Traumatol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"S\u00e1nchez-Somolinos, M., D\u00edaz-Navarro, M., Benjumea, A., Tormo, M., Matas, J., Vaquero, J., Mu\u00f1oz, P., Sanz-Ru\u00edz, P., and Guembe, M. (2022). Determination of the Elution Capacity of Dalbavancin in Bone Cements: New Alternative for the Treatment of Biofilm-Related Peri-Prosthetic Joint Infections Based on an In Vitro Study. Antibiotics, 11.","DOI":"10.3390\/antibiotics11101300"},{"key":"ref_14","first-page":"1222","article-title":"The current and future off-label uses of dalbavancin: A narrative review","volume":"27","author":"Fiore","year":"2023","journal-title":"Eur. Rev. Med. Pharm. Sci."},{"key":"ref_15","first-page":"532","article-title":"Dalbavancin for treating prosthetic joint infections caused by Gram-positive bacteria: A proposal for a low dose strategy. A retrospective cohort study","volume":"32","year":"2019","journal-title":"Rev. Espa\u00f1ola Quimioter."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1016\/j.ijantimicag.2017.11.008","article-title":"Dalbavancin in the treatment of different gram-positive infections: A real-life experience","volume":"51","author":"Bouza","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"749685","DOI":"10.3389\/fmicb.2021.749685","article-title":"Biofilm-Related Infections in Gram-Positive Bacteria and the Potential Role of the Long-Acting Agent Dalbavancin","volume":"12","author":"Oliva","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1016\/j.ijantimicag.2017.12.011","article-title":"Dalbavancin in-vitro activity obtained against Gram-positive clinical isolates causing bone and joint infections in US and European hospitals (2011\u20132016)","volume":"51","author":"Pfaller","year":"2018","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e0035122","DOI":"10.1128\/spectrum.00351-22","article-title":"Role of Extracellular DNA in Dalbavancin Activity against Methicillin-Resistant Staphylococcus aureus (MRSA) Biofilms in Patients with Skin and Soft Tissue Infections","volume":"10","author":"Sivori","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_20","first-page":"114901","article-title":"In Vitro time-kill kinetics of dalbavancin against Staphylococcus spp. biofilms over prolonged exposure times","volume":"96","author":"Ceccherini","year":"2019","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1849","DOI":"10.1128\/AAC.04550-14","article-title":"Extended-Duration Dosing and Distribution of Dalbavancin into Bone and Articular Tissue","volume":"59","author":"Dunne","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2182","DOI":"10.1093\/jac\/dkaa163","article-title":"Efficacy of dalbavancin against MRSA biofilms in a rat model of orthopaedic implant-associated infection","volume":"75","author":"Silva","year":"2020","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"23425","DOI":"10.1038\/s41598-021-02709-x","article-title":"In Vitro additive effects of dalbavancin and rifampicin against biofilm of Staphylococcus aureus","volume":"11","author":"Jacob","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"106664","DOI":"10.1016\/j.ijantimicag.2022.106664","article-title":"Comparative efficacy of dalbavancin alone and with rifampicin against in vitro biofilms in a pharmacodynamic model with methicillin-resistant Staphylococcus aureus","volume":"60","author":"Benavent","year":"2022","journal-title":"Int. J. Antimicrob. Agents"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"3724","DOI":"10.1016\/j.arth.2020.06.045","article-title":"Is Dual Antibiotic-Loaded Bone Cement More Effective and Cost-Efficient Than a Single Antibiotic-Loaded Bone Cement to Reduce the Risk of Prosthetic Joint Infection in Aseptic Revision Knee Arthroplasty?","volume":"35","author":"Vaquero","year":"2020","journal-title":"J. Arthroplast."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/9\/1445\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:50:28Z","timestamp":1760129428000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/9\/1445"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,13]]},"references-count":25,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["antibiotics12091445"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12091445","relation":{},"ISSN":["2079-6382"],"issn-type":[{"type":"electronic","value":"2079-6382"}],"subject":[],"published":{"date-parts":[[2023,9,13]]}}}