{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T19:59:12Z","timestamp":1793908752996,"version":"4.8.0"},"reference-count":109,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T00:00:00Z","timestamp":1694649600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Indian Council of Agricultural Research (ICAR)"},{"name":"CGIAR Research Programme on Agriculture for Nutrition and Health"},{"name":"CGIAR research initiative One Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>The consumption of milk contaminated with antibiotic-resistant bacteria poses a significant health threat to humans. This study aimed to investigate the prevalence of Enterobacteriaceae producing \u03b2-lactamases (ESBL, MBL, and AmpC) in cow and buffalo milk samples from two Indian states, Haryana and Assam. A total of 401 milk samples were collected from dairy farmers and vendors in the specified districts. Microbiological assays, antibiotic susceptibility testing, and PCR-based genotyping were employed to analyze 421 Gram-negative bacterial isolates. The overall prevalence of \u03b2-lactamase genes was 10% (confidence interval (CI) (7\u201313)), with higher rates in Haryana (13%, CI (9\u201319)) compared to Assam (7%, CI (4\u201311)). The identified \u03b2-lactamase genes in isolates were blaCMY, blaMOX, blaFOX, blaEBC, and blaDHA, associated with AmpC production. Additionally, blaCTX-M1, blaSHV, and blaTEM were detected as ESBL producers, while blaVIM, blaIMP, blaSPM, blaSIM, and blaGIM were identified as MBL producers. Notably, Shigella spp. were the dominant \u03b2-lactamase producers among identified Enterobacteriaceae. This study highlights the presence of various prevalent \u03b2-lactamase genes in milk isolates, indicating the potential risk of antimicrobial-resistant bacteria in dairy products. The presence of \u03b2-lactam resistance raises concern as this could restrict antibiotic options for treatment. The discordance between genotypic and phenotypic methods emphasizes the necessity for comprehensive approaches that integrate both techniques to accurately assess antibiotic resistance. Urgent collaborative action incorporating rational and regulated use of antibiotics across the dairy value chain is required to address the global challenge of \u03b2-lactam resistance.<\/jats:p>","DOI":"10.3390\/antibiotics12091449","type":"journal-article","created":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T02:54:13Z","timestamp":1694746453000},"page":"1449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Analyses of Extended-Spectrum-\u03b2-Lactamase, Metallo-\u03b2-Lactamase, and AmpC-\u03b2-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9184-449X","authenticated-orcid":false,"given":"Tushar Kumar","family":"Dey","sequence":"first","affiliation":[{"name":"Department of Biosciences, International Livestock Research Institute, Nairobi 00100, Kenya"},{"name":"Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden"},{"name":"ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1175-0398","authenticated-orcid":false,"given":"Johanna Frida","family":"Lindahl","sequence":"additional","affiliation":[{"name":"Department of Biosciences, International Livestock Research Institute, Nairobi 00100, Kenya"},{"name":"Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden"},{"name":"Department of Clinical Sciences, Swedish University of Agricultural Sciences, 75007 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8608-6551","authenticated-orcid":false,"given":"\u00c5ke","family":"Lundkvist","sequence":"additional","affiliation":[{"name":"Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0195-9489","authenticated-orcid":false,"given":"Delia","family":"Grace","sequence":"additional","affiliation":[{"name":"Department of Biosciences, International Livestock Research Institute, Nairobi 00100, Kenya"},{"name":"Food and Markets Department, Natural Resources Institute, Chatham Maritime ME4 4TB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8120-224X","authenticated-orcid":false,"given":"Ram Pratim","family":"Deka","sequence":"additional","affiliation":[{"name":"International Livestock Research Institute, Regional Office for South Asia, New Delhi 110012, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajeswari","family":"Shome","sequence":"additional","affiliation":[{"name":"ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samiran","family":"Bandyopadhyay","sequence":"additional","affiliation":[{"name":"Eastern Regional Station, ICAR-Indian Veterinary Research Institute, Kolkata 700037, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh Kumar","family":"Goyal","sequence":"additional","affiliation":[{"name":"Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0268-9927","authenticated-orcid":false,"given":"Garima","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Biosciences, International Livestock Research Institute, Nairobi 00100, Kenya"},{"name":"Zoonosis Science Center, Department of Medical Biochemistry and Microbiology, Uppsala University, 75123 Uppsala, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibek Ranjan","family":"Shome","sequence":"additional","affiliation":[{"name":"ICAR-National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru 560064, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,14]]},"reference":[{"key":"ref_1","unstructured":"FAOSTAT (2023, April 27). Ministry of Fisheries, Animal Husbandry & Dairying, Available online: https:\/\/pib.gov.in\/PressReleaseIframePage.aspx?PRID=1897084."},{"key":"ref_2","unstructured":"FAO (2022, September 30). Dairy Production and Products. Food and Agriculture Organization of the United Nations, Available online: https:\/\/www.fao.org\/dairy-production-products\/products\/en\/."},{"key":"ref_3","unstructured":"Patil, B.R. (2006). Dynamics of Livestock Development in Gujarat, India: Experiences of an Indian NGO, Wageningen University and Research."},{"key":"ref_4","unstructured":"Staal, S.J., Pratt, A.N., and Jabbar, M.A. (2010). A Comparison of Dairy Policies and Development in South Asia and East Africa, ILRI."},{"key":"ref_5","unstructured":"Rao, C.K., Bachhman, F., Sharma, V., Venkataramaiah, P., Panda, J., and Rathinam, R. (2014). Smallholder Dairy Value Chain Development in India and Select States (Assam and Bihar): Situation Analysis and Trends. ILRI Proj. Rep., 10\u201332."},{"key":"ref_6","unstructured":"Deka, R.P., Bayan, B., Baltenweck, I., and Grace, D. (2019). Mapping of Informal Dairy Value Chain Actors in Selected Districts of Assam, The International Livestock Research Institute."},{"key":"ref_7","first-page":"1","article-title":"Mapping of Milk Processing Units in Organized Sector: A Case Study for Haryana","volume":"6","author":"Sharma","year":"2020","journal-title":"Int. J. Environ. Agric. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1089\/fpd.2014.1872","article-title":"Molecular Serotyping and Pathogenic Potential of Listeria Monocytogenes Isolated from Milk and Milk Products in Tamil Nadu, India","volume":"12","author":"Karthikeyan","year":"2015","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"EFSA Panel on Biological Hazards (BIOHAZ), Ricci, A., Allende, A., Bolton, D., Chemaly, M., Davies, R., Fern\u00e1ndez Esc\u00e1mez, P.S., Girones, R., Koutsoumanis, K., and Lindqvist, R. (2017). Risk for the Development of Antimicrobial Resistance (AMR) Due to Feeding of Calves with Milk Containing Residues of Antibiotics. EFSA J., 15, 4665.","DOI":"10.2903\/j.efsa.2017.4665"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"5649","DOI":"10.1073\/pnas.1503141112","article-title":"Global Trends in Antimicrobial Use in Food Animals","volume":"112","author":"Brower","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1057\/s41599-018-0152-2","article-title":"Pharming Animals: A Global History of Antibiotics in Food Production (1935\u20132017)","volume":"4","author":"Kirchhelle","year":"2018","journal-title":"Palgrave Commun."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"155","DOI":"10.2147\/IDR.S384776","article-title":"Is AMR in Dairy Products a Threat to Human Health? An Updated Review on the Origin, Prevention, Treatment, and Economic Impacts of Subclinical Mastitis","volume":"16","author":"Paramasivam","year":"2023","journal-title":"Infect. Drug Resist."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fvets.2017.00237","article-title":"Antimicrobial Resistance: Its Surveillance, Impact, and Alternative Management Strategies in Dairy Animals","volume":"4","author":"Sharma","year":"2018","journal-title":"Front. Vet. Sci."},{"key":"ref_14","unstructured":"Wolfenson, K.D.M. (2013). Coping with the Food and Agriculture Challenge: Smallholders\u2019 Agenda, Food and Agriculture Organisation of the United Nations."},{"key":"ref_15","unstructured":"WHO (2014). Antimicrobial Resistance: Global Report on Surveillance, WHO. Available online: https:\/\/apps.who.int\/iris\/handle\/10665\/112642."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/j.cvfa.2018.06.002","article-title":"Making Antibiotic Treatment Decisions for Clinical Mastitis","volume":"34","author":"Ruegg","year":"2018","journal-title":"Vet. Clin. Food Anim. Pract."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Berg\u0161pica, I., Kaprou, G., Alexa, E.A., Prieto, M., and Alvarez-Ord\u00f3\u00f1ez, A. (2020). Extended Spectrum \u03b2-Lactamase (ESBL) Producing Escherichia coli in Pigs and Pork Meat in the European Union. Antibiotics, 9.","DOI":"10.3390\/antibiotics9100678"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"263","DOI":"10.4103\/0974-777X.68531","article-title":"Extended-Spectrum \u03b2-Lactamases in Gram Negative Bacteria","volume":"2","author":"Rawat","year":"2010","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"2705","DOI":"10.1128\/AAC.05598-11","article-title":"Molecular Characterization of Extended-Spectrum-\u03b2-Lactamase- Producing and Plasmid-Mediated AmpC \u03b2-Lactamase-Producing Escherichia coli Isolated from Stray Dogs in South Korea","volume":"56","author":"Tamang","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_20","first-page":"15","article-title":"Carbapenem Resistance: Overview of the Problem and Future Perspectives","volume":"3","author":"Meletis","year":"2016","journal-title":"Ther. Adv. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1002\/phar.1636","article-title":"Infections Caused by Resistant Gram-negative Bacteria: Epidemiology and Management","volume":"35","author":"Kaye","year":"2015","journal-title":"Pharmacother. J. Hum. Pharmacol. Drug Ther."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11908-015-0510-9","article-title":"Carbapenem-Resistant Enterobacteriaceae Infections in Children","volume":"18","author":"Chiotos","year":"2016","journal-title":"Curr. Infect. Dis. Rep."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1030","DOI":"10.1093\/cid\/cis1043","article-title":"Public Health Risks of Enterobacterial Isolates Producing Extended-Spectrum \u03b2-Lactamases or AmpC \u03b2-Lactamases in Food and Food-Producing Animals: An EU Perspective of Epidemiology, Analytical Methods, Risk Factors, and Control Options","volume":"56","author":"Liebana","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1111\/jam.13169","article-title":"Colistin and Tigecycline Resistance in Carbapenemase-producing Gram-negative Bacteria: Emerging Resistance Mechanisms and Detection Methods","volume":"121","author":"Govinden","year":"2016","journal-title":"J. Appl. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Elshamy, A.A., Saleh, S.E., Alshahrani, M.Y., Aboshanab, K.M., Aboulwafa, M.M., and Hassouna, N.A. (2021). OXA-48 Carbapenemase-Encoding Transferable Plasmids of Klebsiella pneumoniae Recovered from Egyptian Patients Suffering from Complicated Urinary Tract Infections. Biology, 10.","DOI":"10.3390\/biology10090889"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1097\/NT.0000000000000108","article-title":"Raw Milk Consumption: Risks and Benefits","volume":"50","author":"Lucey","year":"2015","journal-title":"Nutr. Today"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1089\/fpd.2010.0730","article-title":"Impact of Antibiotic Use in Adult Dairy Cows on Antimicrobial Resistance of Veterinary and Human Pathogens: A Comprehensive Review","volume":"8","author":"Oliver","year":"2011","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"984","DOI":"10.14202\/vetworld.2019.984-993","article-title":"Antibiotic-resistant Escherichia coli and Salmonella spp. associated with dairy cattle and farm environment having public health significance","volume":"12","author":"Sobur","year":"2019","journal-title":"Vet. World"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"54359","DOI":"10.1007\/s11356-022-19495-2","article-title":"Epidemiological study on Listeria monocytogenes in Egyptian dairy cattle farms\u2019 insights into genetic diversity of multi-antibiotic-resistant strains by ERIC-PCR","volume":"29","author":"Elsayed","year":"2022","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Serweci\u0144ska, L. (2020). Antimicrobials and antibiotic-resistant bacteria: A risk to the environment and to public health. Water, 12.","DOI":"10.3390\/w12123313"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3878","DOI":"10.1038\/s41598-022-07845-6","article-title":"High prevalence of antibiotic resistance in pathogenic foodborne bacteria isolated from bovine milk","volume":"12","author":"Hassani","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Montso, K.P., Dlamini, S.B., Kumar, A., and Ateba, C.N. (2019). Antimicrobial resistance factors of extended-spectrum beta-lactamases producing Escherichia coli and Klebsiella pneumoniae isolated from Cattle Farms and Raw Beef in North-West Province, South Africa. Biomed Res. Int.","DOI":"10.1155\/2019\/4318306"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1016\/j.biortech.2013.12.007","article-title":"Prevalence and persistence of potentially pathogenic and antibiotic resistant bacteria during anaerobic digestion treatment of cattle manure","volume":"153","author":"Resende","year":"2014","journal-title":"Bioresour. Technol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1111\/j.1365-2672.2012.05244.x","article-title":"Milk Microbiome Signatures of Subclinical Mastitis-affected Cattle Analysed by Shotgun Sequencing","volume":"112","author":"Bhatt","year":"2012","journal-title":"J. Appl. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13099-016-0119-z","article-title":"Genome Sequence of OXA-23 Producing Acinetobacter baumannii IHIT7853, a Carbapenem-Resistant Strain from a Cat Belonging to International Clone IC1","volume":"8","author":"Ewers","year":"2016","journal-title":"Gut Pathog."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1007\/s00203-023-03524-w","article-title":"Antibiotic Resistance Profiles on Pathogenic Bacteria in the Brazilian Environments","volume":"205","author":"Campos","year":"2023","journal-title":"Arch. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12916-019-1314-9","article-title":"Population-level mathematical modeling of antimicrobial resistance: A systematic review","volume":"17","author":"Niewiadomska","year":"2019","journal-title":"BMC Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1007\/s11250-022-03077-y","article-title":"Udder and teat morphometry in relation to clinical mastitis in dairy cows","volume":"54","author":"Sinha","year":"2022","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1007\/s11250-017-1253-4","article-title":"Mastitis effects on reproductive performance in dairy cattle: A review","volume":"49","author":"Kumar","year":"2017","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1007\/s11250-014-0718-y","article-title":"A systematic review and meta-analysis of prevalence of subclinical mastitis in dairy cows in India","volume":"47","author":"Bangar","year":"2015","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.rvsc.2019.05.012","article-title":"Microbiological profiles in clinical and subclinical cases of mastitis in milking Jafarabadi buffalo","volume":"125","author":"Patel","year":"2019","journal-title":"Res. Vet. Sci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s11259-021-09838-8","article-title":"Bacteria from bovine clinical mastitis showed multiple drug resistance","volume":"46","author":"Awandkar","year":"2022","journal-title":"Vet. Res. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.3923\/pjbs.2013.1653.1661","article-title":"Trends in diagnosis and control of bovine mastitis: A review","volume":"16","author":"Deb","year":"2013","journal-title":"Pak. J. Biol. Sci."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.rvsc.2021.04.021","article-title":"Global and countrywide prevalence of subclinical and clinical mastitis in dairy cattle and buffaloes by systematic review and meta-analysis","volume":"136","author":"Krishnamoorthy","year":"2021","journal-title":"Res. Vet. Sci."},{"key":"ref_45","unstructured":"(2015). Performance Standards for Antimicrobial Susceptibility Testing. Standard No. CLSI M100-S25. Twenty-Fifth Informational Supplement."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"444","DOI":"10.1016\/j.bjm.2015.11.034","article-title":"Occurrence and Characteristics of Extended Spectrum Beta-Lactamases-Producing Enterobacteriaceae from Foods of Animal Origin","volume":"47","author":"Tekiner","year":"2016","journal-title":"Braz. J. Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1688","DOI":"10.1099\/jmm.0.000620","article-title":"OXA-48 and CTX-M-15 Extended-Spectrum Beta-Lactamases in Raw Milk in Lebanon: Epidemic Spread of Dominant Klebsiella pneumoniae Clones","volume":"66","author":"Diab","year":"2017","journal-title":"J. Med. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"3435","DOI":"10.1073\/pnas.1222743110","article-title":"Diverse and Abundant Antibiotic Resistance Genes in Chinese Swine Farms","volume":"110","author":"Zhu","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Kamaruzzaman, E.A., Aziz, S.A., Bitrus, A.A., Zakaria, Z., and Hassan, L. (2020). Occurrence and Characteristics of Extended-Spectrum \u03b2-Lactamase-Producing Escherichia Coli from Dairy Cattle, Milk, and Farm Environments in Peninsular Malaysia. Pathogens, 9.","DOI":"10.20944\/preprints202009.0485.v1"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Dey, T.K., Shome, B.R., Bandyopadhyay, S., Goyal, N.K., Lundkvist, \u00c5., Deka, R.P., Shome, R., Venugopal, N., Grace, D., and Sharma, G. (2023). Molecular Characterization of Methicillin-Resistant Staphylococci from the Dairy Value Chain in Two Indian States. Pathogens, 12.","DOI":"10.3390\/pathogens12020344"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"271","DOI":"10.3201\/eid0902.020026","article-title":"Dual Infection by Dengue Virus and Shigellas onnei in Patient Returning from India","volume":"9","author":"Charrel","year":"2003","journal-title":"Emerg. Infect. Dis."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1016\/j.ijfoodmicro.2014.11.013","article-title":"Molecular Characterization of Multidrug-Resistant Shigella spp. of Food Origin","volume":"194","author":"Ahmed","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"6362","DOI":"10.1002\/fsn3.2603","article-title":"Genetic Diversity and Antibiotic Resistance of Shigella spp. Isolates from Food Products","volume":"9","author":"Pakbin","year":"2021","journal-title":"Food Sci. Nutr."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1056036","DOI":"10.3389\/fimmu.2022.1056036","article-title":"Viral Infectious Diseases Severity: Co-Presence of Transcriptionally Active Microbes (TAMs) Can Play an Integral Role for Disease Severity","volume":"13","author":"Yadav","year":"2022","journal-title":"Front. Immunol."},{"key":"ref_55","unstructured":"Baylis, C., Uyttendaele, M., Joosten, H., and Davies, A. (2011). The Enterobacteriaceae and Their Significance to the Food Industry, ILSI Europe."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1590\/0074-0276140196","article-title":"Community-Acquired Invasive Liver Abscess Syndrome Caused by a K1 Serotype Klebsiella pneumoniae Isolate in Brazil: A Case Report of Hypervirulent ST23","volume":"109","author":"Coutinho","year":"2014","journal-title":"Mem\u00f3rias Inst. Oswaldo Cruz"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1016\/j.meegid.2016.07.032","article-title":"Molecular Signature of Extended Spectrum \u03b2-Lactamase Producing Klebsiella pneumoniae Isolated from Bovine Milk in Eastern and North-Eastern India","volume":"44","author":"Koovapra","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.ijfoodmicro.2016.08.036","article-title":"Extended-Spectrum \u03b2-Lactamase Producing Enterobacteriaceae in Bulk Tank Milk from German Dairy Farms","volume":"238","author":"Odenthal","year":"2016","journal-title":"Int. J. Food Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1016\/j.cmi.2017.01.013","article-title":"Extended-Spectrum \u03b2-Lactamase\/AmpC-and Carbapenemase-Producing Enterobacteriaceae in Animals: A Threat for Humans?","volume":"23","author":"Madec","year":"2017","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Shukla, S., Desai, S., Bagchi, A., Singh, P., Joshi, M., Joshi, C., Patankar, J., Maheshwari, G., Rajni, E., and Shah, M. (2023). Diversity and Distribution of \u03b2-Lactamase Genes Circulating in Indian Isolates of Multidrug-Resistant Klebsiella pneumoniae. Antibiotics, 12.","DOI":"10.20944\/preprints202301.0350.v1"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1128\/JCM.00219-10","article-title":"Extended-Spectrum-\u03b2-Lactamase, AmpC, and Carbapenemase Issues","volume":"48","author":"Thomson","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/j.mimet.2005.03.018","article-title":"Identification of plasmids by PCR-based replicon typing","volume":"63","author":"Carattoli","year":"2005","journal-title":"J. Microbiol. Methods"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/tbed.12119","article-title":"Detection of New Delhi Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase Genes in Escherichia coli Isolated from Mastitic Milk Samples","volume":"60","author":"Ghatak","year":"2013","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"143","DOI":"10.30954\/2277-940X.01.2020.21","article-title":"Isolation and Molecular Characterization of Extended Spectrum Beta Lactamase Producing Escherichia coli from Milk","volume":"10","author":"Paghdar","year":"2020","journal-title":"J. Anim. Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"200","DOI":"10.14202\/vetworld.2021.200-209","article-title":"Molecular Characterization of Extended-Spectrum \u03b2-Lactamase-Producing Escherichia coli Isolated from Postpartum Uterine Infection in Dairy Cattle in India","volume":"14","author":"Agrawal","year":"2021","journal-title":"Vet. World"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Deb, R., Chaudhary, P., and De, S. (2022). CRISPR\/Cas9 Cassette Targeting Escherichia coli BlaCTX-M Specific Gene of Mastitis Cow Milk Origin Can Alter the Antibiotic Resistant Phenotype for Cefotaxime. Anim. Biotechnol., 1\u20136.","DOI":"10.1080\/10495398.2022.2053695"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2150","DOI":"10.1007\/s13197-021-05226-x","article-title":"Occurrence of Multiple Drug-Resistant Shiga Toxigenic Escherichia coli in Raw Milk Samples Collected from Retail Outlets in South India","volume":"59","author":"Joseph","year":"2022","journal-title":"J. Food Sci. Technol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1089\/mdr.2018.0414","article-title":"Alliance of efflux pumps with \u03b2-lactamases in multidrug-resistant Klebsiella pneumoniae isolates","volume":"25","author":"Maurya","year":"2019","journal-title":"Microb. Drug Resist."},{"key":"ref_69","first-page":"1","article-title":"Detection of high prevalence of TEM. SHV\/CTX-M Genes in ESBL Producing and Multidrug Resistant Klebsiella pneumoniae and Klebsiella oxytoca","volume":"4","author":"Diagbouga","year":"2016","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_70","unstructured":"Hassoubah, S.A. (2023). Genetic analysis of multidrug-resistant and AmpC-producing Citrobacter freundii. Eur. Rev. Med. Pharmacol. Sci., 27."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.3389\/fmicb.2018.02299","article-title":"Prevalence and emergence of extended-spectrum cephalosporin-, carbapenem- and colistin-resistant gram negative bacteria of animal origin in the Mediterranean basin","volume":"9","author":"Dandachi","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Karkaba, A. (2017). Occurrence and distribution of extended spectrum \u03b2-lactamase and AmpC-\u03b2-lactamase-producing Enterobacteriaceae and methicillin resistant Staphylococcus aureus in companion animals in New Zealand: A thesis presented in partial fulfilment of the requirement. [Ph.D. Thesis, Massey University].","DOI":"10.1080\/00480169.2016.1271730"},{"key":"ref_73","unstructured":"Wawira, C.M. (2020). Phenotypic and Genotypic Characterization of Uropathogenic Escherichia Coli Isolates from Patients in Selected Hospitals in Kenya. [Ph.D. Thesis, Maseno University]."},{"key":"ref_74","first-page":"289","article-title":"High occurrence of blaCMY-1 AmpC lactamase producing Escherichia coli in cases of complicated urinary tract infection (UTI) from a tertiary health care centre in north India","volume":"136","author":"Taneja","year":"2012","journal-title":"Indian J. Med. Res."},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Matloko, K., Fri, J., Ateba, T.P., Molale-Tom, L.G., and Ateba, C.N. (2021). Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the bla ACC resistance determinant. PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0253647"},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"2716","DOI":"10.1128\/AAC.45.10.2716-2722.2001","article-title":"Evidence for transfer of CMY-2 AmpC \u03b2-lactamase plasmids between Escherichia coli and Salmonella isolates from food animals and humans","volume":"45","author":"Winokur","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1016\/j.plasmid.2018.09.010","article-title":"Plasmid-mediated resistance is going wild","volume":"99","author":"Dolejska","year":"2018","journal-title":"Plasmid"},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"585","DOI":"10.1089\/fpd.2014.1895","article-title":"Extended-spectrum \u03b2-lactamase (ESBL)\u2013producing Klebsiella pneumoniae in bulk tank milk from dairy farms in Indonesia","volume":"12","author":"Sudarwanto","year":"2015","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13756-017-0222-z","article-title":"High burden of antimicrobial resistance among gram negative bacteria causing healthcare associated infections in a critical care unit of Nepal","volume":"6","author":"Parajuli","year":"2017","journal-title":"Antimicrob. Resist. Infect. Control."},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Bassetti, M., Vena, A., Sepulcri, C., Giacobbe, D.R., and Peghin, M. (2020). Treatment of bloodstream infections due to Gram-negative bacteria with difficult-to-treat resistance. Antibiotics., 9.","DOI":"10.3390\/antibiotics9090632"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1038\/s41581-019-0150-7","article-title":"Antimicrobial resistance in nephrology","volume":"15","author":"Wang","year":"2019","journal-title":"Nat. Rev. Nephrol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"33","DOI":"10.4103\/0974-2727.78561","article-title":"Comparison of Disc Diffusion Methods for the Detection of Extended-Spectrum Beta Lactamase-Producing Enterobacteriaceae","volume":"3","author":"Giriyapur","year":"2011","journal-title":"J. Lab. Physicians"},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"91396","DOI":"10.1371\/journal.pone.0091396","article-title":"Detection and occurrence of plasmid-mediated AmpC in highly resistant gram-negative rods","volume":"9","author":"Reuland","year":"2014","journal-title":"PLoS ONE"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pmed.1001974","article-title":"Antibiotic Resistance in India: Drivers and Opportunities for Action","volume":"13","author":"Laxminarayan","year":"2016","journal-title":"PLoS Med."},{"key":"ref_85","doi-asserted-by":"crossref","unstructured":"Silva, N., Carvalho, I., Currie, C., Sousa, M., Igrejas, G., and Poeta, P. (2019). Extended-Spectrum-\u03b2-Lactamase and Carbapenemase-Producing Enterobacteriaceae in Food-Producing Animals in Europe: An Impact on Public Health?. Antibiot. Drug Resist., 261\u2013273.","DOI":"10.1002\/9781119282549.ch12"},{"key":"ref_86","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23.","DOI":"10.3390\/molecules23040795"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1136\/bmjgh-2019-002104","article-title":"Tackling Antimicrobial Resistance in Low-Income and Middle-Income Countries","volume":"4","author":"Pokharel","year":"2019","journal-title":"BMJ Glob. Health"},{"key":"ref_88","first-page":"1590","article-title":"Quick Fix for Care, Productivity, Hygiene and Inequality: Reframing the Entrenched Problem of Antibiotic Overuse","volume":"4","author":"Willis","year":"2019","journal-title":"BMJ Glob. Health"},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"EFSA (2012). Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in Salmonella, Campylobacter and indicator Escherichia coli and Enterococcus spp. bacteria transmitted through food. Eur. Food Saf. Auth. J., 10, 2742.","DOI":"10.2903\/j.efsa.2012.2742"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1016\/bs.aambs.2020.03.001","article-title":"Antimicrobial Resistance Genes in Bacteria from Animal-Based Foods","volume":"112","author":"Ferrari","year":"2020","journal-title":"Adv. Appl. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"4022","DOI":"10.1016\/j.scitotenv.2009.04.010","article-title":"Antimicrobial Resistance of Listeria Monocytogenes Isolated from Dairy-Based Food Products","volume":"407","author":"Harakeh","year":"2009","journal-title":"Sci. Total Environ."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.tifs.2018.10.007","article-title":"Hazards of a \u2018Healthy\u2019 Trend? An Appraisal of the Risks of Raw Milk Consumption and the Potential of Novel Treatment Technologies to Serve as Alternatives to Pasteurization","volume":"82","author":"Alegbeleye","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_93","unstructured":"FDA (2022, December 22). Antimicrobials Sold or Distributed for Use in Food-Producing Animals, Available online: https:\/\/www.fda.gov\/files\/aboutfda\/published\/2015-Summary-Report-on-Antimicrobials-Sold-or-Distributed-for-Use-in-Food-Producing-Animals.pdf."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1016\/S2542-5196(17)30141-9","article-title":"Restricting the use of antibiotics in food-producing animals and its associations with antibiotic resistance in food-producing animals and human beings: A systematic review and meta-analysis","volume":"1","author":"Tang","year":"2017","journal-title":"Lancet Planet Health"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"812","DOI":"10.1126\/science.1185383","article-title":"Food Security: The Challenge of Feeding 9 Billion People","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"742","DOI":"10.4315\/0362-028X-61.6.742","article-title":"Antimicrobial drug residues in milk and meat: Causes, concerns, prevalence, regulations, tests, and test performance","volume":"61","author":"Mitchell","year":"1998","journal-title":"J. Food Prot."},{"key":"ref_97","unstructured":"Nyokabi, S.N. (2015). Biosecurity measures in meat and milk value chains: A study in Bura Sub-county, Kenya. [Ph.D. Thesis, University of Hohenheim]."},{"key":"ref_98","unstructured":"DAHD (2022, November 05). Department of Animal Husbandry, Dairying and Fisheries. Ministry of Agriculture. 20th Livestock Census, All India Report 2019. Available online: http:\/\/dahd.nic.in\/dahd\/writeData\/livestock."},{"key":"ref_99","first-page":"9","article-title":"Practical Issues in Calculating the Sample Size for Prevalence Studies","volume":"1","author":"Naing","year":"2006","journal-title":"Arch. Orofac. Sci."},{"key":"ref_100","unstructured":"Hudzicki, J. (2009). Kirby-Bauer Disk Diffusion Susceptibility Test Protocol Author Information. Am. Soc. Microbiol., 1\u201313. Available online: https:\/\/www.asm.org\/Protocols\/Kirby-Bauer-Disk-Diffusion-Susceptibility-Test-Pro."},{"key":"ref_101","first-page":"37","article-title":"Occurrence of Antibiotic Resistance in Bacteria Isolated from Milk of Dairy Cows in Small-Holder Farms in Juja Sub-County, Kenya","volume":"9","author":"Kagira","year":"2022","journal-title":"Asian J. Res. Anim. Vet. Sci."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1128\/AAC.49.8.3533-3537.2005","article-title":"Extended-Spectrum-\u03b2-Lactamase-Producing Escherichia coli Strains Isolated from Farm Animals from 1999 to 2002: Report from the Japanese Veterinary Antimicrobial Resistance Monitoring Program","volume":"49","author":"Kojima","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"2569","DOI":"10.1128\/JCM.41.6.2569-2576.2003","article-title":"Comparative Study Using Type Strains and Clinical and Food Isolates to Examine Hemolytic Activity and Occurrence of the Cyl Operon in Enterococci","volume":"41","author":"Semedo","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"5715","DOI":"10.1128\/JCM.42.12.5715-5721.2004","article-title":"Phenotypic and Molecular Detection of CTX-M-Beta-Lactamases Produced by E. coli and Klebsiella spp.","volume":"42","author":"Johann","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"544","DOI":"10.1128\/JCM.01728-06","article-title":"Rapid Detection and Identification of Metallo-\u03b2-Lactamase-Encoding Genes by Multiplex Real-Time PCR Assay and Melt Curve Analysis","volume":"45","author":"Mendes","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"2153","DOI":"10.1128\/JCM.40.6.2153-2162.2002","article-title":"Detection of plasmid-mediated AmpC \u03b2-lactamase genes in clinical isolates by using multiplex PCR","volume":"40","author":"Hanson","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"492","DOI":"10.4161\/epi.19742","article-title":"Histone H3K14 and H4K8 Hyperacetylation Is Associated with Escherichia coli Induced Mastitis in Mice","volume":"7","author":"Modak","year":"2012","journal-title":"Epigenetics"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"832","DOI":"10.56093\/ijans.v85i8.50931","article-title":"Duplex PCR for Specific Detection of Escherichia coli and Its Differentiation from Other Enterobacteriaceae","volume":"85","author":"Mitra","year":"2015","journal-title":"Indian J. Anim. Sci."},{"key":"ref_109","first-page":"138","article-title":"Differentiation of Klebsiella pneumoniae and K. oxytoca by Multiplex Polymerase Chain Reaction","volume":"9","author":"Chander","year":"2011","journal-title":"Int. J. Appl. Res. Vet. Med."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/9\/1449\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:51:19Z","timestamp":1760129479000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/9\/1449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,14]]},"references-count":109,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["antibiotics12091449"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12091449","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9,14]]}}}