{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:33:20Z","timestamp":1787027600705,"version":"build-2736575974"},"reference-count":28,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>In this study, we explored the incidence of double J (JJ) contamination of patients who underwent an endourological procedure for urinary stones and ureteral stenosis. We developed a prospective study between January 2019 and December 2021. Ninety-seven patients, 54 male and 43 female, were enrolled. Urine culture was taken during four steps: before stent insertion, a sample from selective renal pelvis catheterization, a sample two days after the JJ insertion and finally, after the stent removal procedure. At the time of the stent removal, 1 cm of proximal and distal ends were cut off and placed in the culture for bacterial evaluation. Cohen\u2019s kappa coefficient value (k) and concordance rates of microbiological culture results were evaluated. The study group comprised 56% of male patients. Proximal and distal stent cultures were positive in 81 and 78 patients. The concordance rate of microbiological cultures between proximal and distal double J stent is 88% (k 0.6). The most common pathogens isolated from urine and stent cultures were Enterococcus spp. in 52 cases and Klebsiella spp. in 27 cases.<\/jats:p>","DOI":"10.3390\/antibiotics12101512","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T11:58:57Z","timestamp":1696420737000},"page":"1512","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of Bacterial Stent Colonization: The Role of Urine and Device Microbiological Cultures"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5797-3351","authenticated-orcid":false,"given":"Gabriele","family":"Tulone","sequence":"first","affiliation":[{"name":"Urology Section, Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90100 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Costanzo","sequence":"additional","affiliation":[{"name":"Urology Section, Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90100 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7355-7625","authenticated-orcid":false,"given":"Nicola","family":"Pavan","sequence":"additional","affiliation":[{"name":"Urology Section, Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90100 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Giaimo","sequence":"additional","affiliation":[{"name":"Urology Section, Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90100 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2812-5553","authenticated-orcid":false,"given":"Francesco","family":"Claps","sequence":"additional","affiliation":[{"name":"Urology Clinic, Department of Medical, Surgical and Health Sciences, University of Trieste, 34127 Trieste, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa Maria Assunta","family":"Fasciana","sequence":"additional","affiliation":[{"name":"Department of Health Promotion, Maternal-Childhood, Internal Medicine of Excellence \u201cG. D\u2019Allesandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Giammanco","sequence":"additional","affiliation":[{"name":"Department of Health Promotion, Maternal-Childhood, Internal Medicine of Excellence \u201cG. D\u2019Allesandro\u201d, University of Palermo, 90127 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0021-0302","authenticated-orcid":false,"given":"Riccardo","family":"Bartoletti","sequence":"additional","affiliation":[{"name":"Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alchiede","family":"Simonato","sequence":"additional","affiliation":[{"name":"Urology Section, Department of Surgical, Oncological and Stomatological Sciences, University of Palermo, 90100 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1038\/nrurol.2014.340","article-title":"Ureteral stent-associated complications\u2014Where we are and where we are going","volume":"12","author":"Lange","year":"2014","journal-title":"Nat. Rev. Urol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1159\/000065563","article-title":"Early and Late Complications of Double Pigtail Ureteral Stent","volume":"69","author":"Damiano","year":"2002","journal-title":"Urol. Int."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1080\/003655901750292006","article-title":"Factors Affecting the Fate of Prolonged Forgotten ?J? Stents","volume":"35","author":"Kehinde","year":"2001","journal-title":"Scand. J. Urol. Nephrol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"84","DOI":"10.1089\/end.2020.0254","article-title":"Double Loop Ureteral Stent Encrustation According to Indwelling Time: Results of a European Multicentric Study","volume":"35","author":"Legrand","year":"2021","journal-title":"J. Endourol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1080\/08927014.2018.1562549","article-title":"Ureteral stent-associated infection and sepsis: Pathogenesis and prevention: A review","volume":"35","author":"Scotland","year":"2019","journal-title":"Biofouling"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1159\/000490621","article-title":"Observations of Bacterial Biofilm on Ureteral Stent and Studies on the Distribution of Pathogenic Bacteria and Drug Resistance","volume":"101","author":"Zhang","year":"2018","journal-title":"Urol. Int."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1089\/end.2011.0385","article-title":"Ureteral Stent Encrustation, Incrustation, and Coloring: Morbidity Related to Indwelling Times","volume":"26","author":"Kawahara","year":"2012","journal-title":"J. Endourol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1111\/ans.16414","article-title":"Association between ureteric stent dwell time and urinary tract infection","volume":"91","author":"Hanna","year":"2020","journal-title":"ANZ J. Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1089\/end.2004.18.891","article-title":"Bacteriology of Urinary Tract Infection Associated with Indwelling J Ureteral Stents","volume":"18","author":"Kehinde","year":"2004","journal-title":"J. Endourol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/j.jpurol.2020.11.004","article-title":"A survey of adult and pediatric urologists on current practice in antibiotic prophylaxis for ureteral stent removal","volume":"17","author":"Berrondo","year":"2020","journal-title":"J. Pediatr. Urol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"71","DOI":"10.4103\/UA.UA_158_17","article-title":"Characteristics of bacterial colonization after indwelling double-J ureteral stents for different time duration","volume":"10","author":"Manzoor","year":"2018","journal-title":"Urol. Ann."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1159\/000019927","article-title":"Bacterial Colonization of Ureteral Stents","volume":"36","author":"Riedl","year":"1999","journal-title":"Eur. Urol."},{"key":"ref_13","first-page":"81","article-title":"Rate of positive urine culture and double\u2013J catheters colonization on the basis of microorganism DNA analysis","volume":"67","author":"Szymkowiak","year":"2014","journal-title":"Central Eur. J. Urol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2077","DOI":"10.1007\/s10096-020-03953-1","article-title":"Urinary tract infection and antibiotic use around ureteral stent insertion for urolithiasis","volume":"39","author":"Souhail","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1005","DOI":"10.1148\/radiographics.22.5.g02se081005","article-title":"Complications of Ureteral Stent Placement","volume":"22","author":"Dyer","year":"2002","journal-title":"RadioGraphics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1089\/end.2005.19.170","article-title":"Impact of a Double-Pigtail Stent on Ureteral Peristalsis in the Porcine Model: Initial Studies Using a Novel Implantable Magnetic Sensor","volume":"19","author":"Venkatesh","year":"2005","journal-title":"J. Endourol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11934-018-0779-y","article-title":"Advances in Ureteral Stent Design and Materials","volume":"19","author":"Mosayyebi","year":"2018","journal-title":"Curr. Urol. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3899","DOI":"10.1016\/j.biomaterials.2011.02.013","article-title":"The biocompatibility and biofilm resistance of implant coatings based on hydrophilic polymer brushes conjugated with antimicrobial peptides","volume":"32","author":"Gao","year":"2011","journal-title":"Biomaterials"},{"key":"ref_19","unstructured":"(2019, April 06). World Health Organization. Antimicrobial Resistance Global Report on Surveillance. Available online: https:\/\/apps.who.int\/iris\/bitstream\/handle\/10665\/112642\/9789241564748_eng.pdf;jssionid=7CD2D037F35036393D8BC456B03B1991?sequence=1."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"100094","DOI":"10.1016\/j.xcrm.2020.100094","article-title":"Ureteral Stent Microbiota Is Associated with Patient Comorbidities but Not Antibiotic Exposure","volume":"1","author":"Al","year":"2020","journal-title":"Cell Rep Med."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1111\/bju.14832","article-title":"Recommended antibiotic prophylaxis regimen in retrograde intrarenal surgery: Evidence from a randomised controlled trial","volume":"124","author":"Zhao","year":"2019","journal-title":"BJU Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"441","DOI":"10.1016\/j.ucl.2015.06.003","article-title":"Update on Antibiotic Prophylaxis for Genitourinary Procedures in Patients with Artificial Joint Replacement and Artificial Heart Valves","volume":"42","author":"Mazur","year":"2015","journal-title":"Urol. Clin. N. Am."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"911","DOI":"10.1136\/jcp.54.12.911","article-title":"ACP Best Practice No 167: The laboratory diagnosis of urinary tract infection","volume":"54","author":"Graham","year":"2001","journal-title":"J. Clin. Pathol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jped.2019.10.006","article-title":"Urinary tract infection in pediatrics: An overview","volume":"96","author":"Silva","year":"2019","journal-title":"J. Pediatr."},{"key":"ref_25","unstructured":"Centers for Disease Control and Prevention (2020, October 13). Urinary Tract Infection (Catheter Associated Urinary Tract Infection [CAUTI] and Non-Catheter Associated Urinary Tract Infection [UTI] and Other Urinary System Infection (USI) Events), Available online: ttps:\/\/www.cdc.gov\/nhsn\/pdfs\/pscmanual\/7psccauticurrent.pdf."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"410","DOI":"10.1111\/j.1574-695X.2010.00686.x","article-title":"Microbial biofilms associated with biliary stent clogging","volume":"59","author":"Guaglianone","year":"2010","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Biswas, M.R., Alzubaidi, M.S., Shah, U., Abd-Alrazaq, A.A., and Shah, Z. (2021). A Scoping Review to Find out Worldwide COVID-19 Vaccine Hesitancy and Its Underlying Determinants. Vaccines, 9.","DOI":"10.3390\/vaccines9111243"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1093\/inthealth\/ihu010","article-title":"Cohen\u2019s kappa for capturing discrimination","volume":"6","author":"Chang","year":"2014","journal-title":"Int. Health"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/10\/1512\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:05:17Z","timestamp":1760130317000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/12\/10\/1512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,4]]},"references-count":28,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["antibiotics12101512"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics12101512","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,10,4]]}}}