{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:15:00Z","timestamp":1790806500886,"version":"4.1.0"},"reference-count":84,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:00:00Z","timestamp":1698105600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III of Spain (ISCIII)","doi-asserted-by":"publisher","award":["FIS project number PI20\/00356"],"award-info":[{"award-number":["FIS project number PI20\/00356"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Regional Development Fund (FEDER)","award":["FIS project number PI20\/00356"],"award-info":[{"award-number":["FIS project number PI20\/00356"]}]},{"name":"Consejer\u00eda de Industria, Innovaci\u00f3n y Empleo","award":["FIS project number PI20\/00356"],"award-info":[{"award-number":["FIS project number PI20\/00356"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Pseudomonas aeruginosa ST274 is an international epidemic high-risk clone, mostly associated with hospital settings and appears to colonize cystic fibrosis (CF) patients worldwide. To understand the relevant mechanisms for its success, the biological and genomic characteristics of 11 ST274-P.\u00a0aeruginosa strains from clinical and non-clinical origins were analyzed. The extensively drug-resistant (XDR\/DTR), the non-susceptible to at least one agent (modR), and the lasR-truncated (by ISPsp7) strains showed a chronic infection phenotype characterized by loss of serotype-specific antigenicity and low motility. Furthermore, the XDR\/DTR and modR strains presented low pigment production and biofilm formation, which were very high in the lasR-truncated strain. Their whole genome sequences were compared with other 14 ST274-P.\u00a0aeruginosa genomes available in the NCBI database, and certain associations have been primarily detected: blaOXA-486 and blaPDC-24 genes, serotype O:3, exoS+\/exoU\u2212 genotype, group V of type IV pili, and pyoverdine locus class II. Other general molecular markers highlight the absence of vqsM and pldA\/tleS genes and the presence of the same mutational pattern in genes involving two-component sensor-regulator systems PmrAB and CreBD, exotoxin A, quorum-sensing RhlI, beta-lactamase expression regulator AmpD, PBP1A, or FusA2 elongation factor G. The proportionated ST274-P. aeruginosa results could serve as the basis for more specific studies focused on better antibiotic stewardship and new therapeutic developments.<\/jats:p>","DOI":"10.3390\/antibiotics12111561","type":"journal-article","created":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T11:34:47Z","timestamp":1698147287000},"page":"1561","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Spread of Pseudomonas aeruginosa ST274 Clone in Different Niches: Resistome, Virulome, and Phylogenetic Relationship"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8763-9389","authenticated-orcid":false,"given":"Gabriela","family":"Chich\u00f3n","sequence":"first","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), C\/Piqueras 98, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3834-4891","authenticated-orcid":false,"given":"Mar\u00eda","family":"L\u00f3pez","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), C\/Piqueras 98, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3329-0203","authenticated-orcid":false,"given":"Mar\u00eda","family":"de Toro","sequence":"additional","affiliation":[{"name":"Plataforma de Gen\u00f3mica y Bioinform\u00e1tica, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), C\/Piqueras 98, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8090-4330","authenticated-orcid":false,"given":"Lidia","family":"Ruiz-Rold\u00e1n","sequence":"additional","affiliation":[{"name":"Joint Research Unit \u201cInfection and Public Health\u201d FISABIO-University of Valencia, Institute for Integrative Systems Biology I2SysBio (CSIC-UV), Av. de Catalunya 21, 46020 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2742-0980","authenticated-orcid":false,"given":"Beatriz","family":"Rojo-Bezares","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), C\/Piqueras 98, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2457-4258","authenticated-orcid":false,"given":"Yolanda","family":"S\u00e1enz","sequence":"additional","affiliation":[{"name":"\u00c1rea de Microbiolog\u00eda Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica de La Rioja (CIBIR), C\/Piqueras 98, 26006 Logro\u00f1o, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1093\/gbe\/evy259","article-title":"The Pseudomonas aeruginosa Pan-Genome Provides New Insights on Its Population Structure, Horizontal Gene Transfer, and Pathogenicity","volume":"11","author":"Freschi","year":"2018","journal-title":"Genome Biol. 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