{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:02:34Z","timestamp":1791482554331,"version":"4.3.4"},"reference-count":42,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:00:00Z","timestamp":1700179200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"S\u00e3o Paulo Research Foundation","doi-asserted-by":"publisher","award":["2020\/11054-8."],"award-info":[{"award-number":["2020\/11054-8."]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>To assess the effect of hygiene protocols and time on the physical\u2013mechanical properties and colony-forming units (CFU) of Candida albicans, Staphylococcus aureus, and Streptococcus mutans on 3D-printed denture resins (SmartPrint and Yller) with extrinsic pigmentation compared to conventional resin (CR). The protocols were evaluated: brushing (B), brushing and immersion in water (W), 0.25% sodium hypochlorite (SH), and 0.15% triclosan (T), simulating 0, 1, 3, and 5 years. The data were analyzed by ANOVA with repeated measurements, ANOVA (Three-way) and Tukey\u2019s post-test, generalized linear model with Bonferroni adjustment, and ANOVA (Two-way) and Tukey\u2019s post-test (\u03b1 = 0.05). The protocols influenced color (p = 0.036) and Knoop hardness (p &lt; 0.001). Surface roughness was influenced by protocols\/resin (p &lt; 0.001) and time\/resin (p = 0.001), and flexural strength by time\/protocols (p = 0.014). C. albicans showed interactions with all factors (p = 0.033). Staphylococcus aureus was affected by protocols (p &lt; 0.001). Streptococcus mutans exhibited no count for SH and T (p &lt; 0.001). Yller resin showed more color changes. The 3D-printed resins displayed lower microhardness, increased roughness, and decreased flexural strength compared to CR with all protocols in a simulated period of 5 years. The indication of printed resins should be restricted to less than 3 years.<\/jats:p>","DOI":"10.3390\/antibiotics12111630","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T00:49:47Z","timestamp":1700182187000},"page":"1630","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Effect of Hygiene Protocols on the Mechanical and Physical Properties of Two 3D-Printed Denture Resins Characterized by Extrinsic Pigmentation as Well as the Mixed Biofilm Formed on the Surface"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3108-8589","authenticated-orcid":false,"given":"Adriana Barbosa","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz Marcatto","family":"Tinelli","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena Mosconi","family":"Clemente","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2761-7660","authenticated-orcid":false,"given":"Beatriz de Camargo","family":"Poker","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0734-0652","authenticated-orcid":false,"given":"Viviane de C\u00e1ssia","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evandro","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1629-2207","authenticated-orcid":false,"given":"Cl\u00e1udia Helena","family":"Silva-Lovato","sequence":"additional","affiliation":[{"name":"Department of Dental Materials and Prosthesis, Ribeir\u00e3o Preto School of Dentistry, University of S\u00e3o Paulo, Caf\u00e9 Avenue S\/N, Ribeir\u00e3o Preto 14040-904, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"234","DOI":"10.4012\/dmj.2014-089","article-title":"Deterioration of polymethyl methacrylate dentures in the oral cavity","volume":"34","author":"Matsuo","year":"2015","journal-title":"Dent. 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