{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:33:20Z","timestamp":1787027600768,"version":"build-2736575974"},"reference-count":67,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T00:00:00Z","timestamp":1701043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japanese Ministry of Health, Labor and Welfare","award":["21KA1004"],"award-info":[{"award-number":["21KA1004"]}]},{"name":"Japanese Ministry of Health, Labor and Welfare","award":["23fk0108604h0903"],"award-info":[{"award-number":["23fk0108604h0903"]}]},{"name":"Japanese Ministry of Health, Labor and Welfare","award":["23fk0108665h0301"],"award-info":[{"award-number":["23fk0108665h0301"]}]},{"name":"Japanese Ministry of Health, Labor and Welfare","award":["22K07067"],"award-info":[{"award-number":["22K07067"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["21KA1004"],"award-info":[{"award-number":["21KA1004"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["23fk0108604h0903"],"award-info":[{"award-number":["23fk0108604h0903"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["23fk0108665h0301"],"award-info":[{"award-number":["23fk0108665h0301"]}]},{"name":"Japan Agency for Medical Research and Development (AMED)","award":["22K07067"],"award-info":[{"award-number":["22K07067"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan","award":["21KA1004"],"award-info":[{"award-number":["21KA1004"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan","award":["23fk0108604h0903"],"award-info":[{"award-number":["23fk0108604h0903"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan","award":["23fk0108665h0301"],"award-info":[{"award-number":["23fk0108665h0301"]}]},{"name":"Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan","award":["22K07067"],"award-info":[{"award-number":["22K07067"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>A total of seventy VanA-type vancomycin-resistant enterococci (VRE) isolates obtained in Taiwan in the early 2000s were retrospectively characterized. Forty isolates were obtained from human patients and thirty from livestock. Of these VRE isolates, twenty-three (57.5%) of the human VRE and thirty (100%) of the livestock VRE were Enterococcus faecalis, and the remaining seventeen (42.5%) of the human VRE were E. faecium. Of the 53 E. faecalis isolates, twenty-two (96%) of the human VRE and thirty (100%) of the livestock VRE exhibited a high level of resistance to vancomycin and sensitivity to teicoplanin. They also had three amino acid substitutions in the N-terminal region of the deduced VanS sequence. The vancomycin resistance of all of the 22 human isolates, and 20 of the 30 livestock isolates, transferred to E. faecalis FA2-2 at a frequency of 10\u22125 to 10\u22123 per donor cell in broth. Each of the transconjugants responded to E. faecalis pheromone (i.e., E. faecalis FA2-2 culture filtrate), indicating that the conjugative plasmids were pheromone-responsive plasmids. Three of the conjugative plasmids originated from human isolates, and five plasmids from livestock isolates were corresponded and classified as type A plasmid. Two plasmids originated from human isolates and six plasmids from livestock isolates were corresponded and classified as type B plasmid. E. faecalis FA2-2 containing either the type A or type B plasmid responded to the synthetic pheromone cAD1. The type A and type B plasmids transferred between E. faecalis FA2-2 and JH2SS at a frequency of about 10\u22122 per donor cell and conferred vancomycin, bacitracin, and erythromycin resistances. The complete DNA sequence of the representative type A plasmid pTW9 (85,068 bp) showed that the plasmid carried a Tn1546-like element encoding vanA-type resistance, erythromycin resistance (ermB), and bacitracin resistance (bcrABDR). The plasmid contained the regulatory region found in the pheromone-responsive plasmid and encoded the genes traA, traD and iad1, which are the key negative regulatory elements, and traE1, a key positive regulator of plasmid pAD1, indicating that plasmid pTW9 was pAD1-type pheromone-responsive plasmid. PFGE analysis of SmaI-digested chromosomal DNAs showed that several E. faecalis strains harboring an identical type A pheromone-responsive plasmid were indistinguishable, and that these were identified both in human and livestock isolates, indicating the transmissions of the VRE strains between livestock and humans. These data showed that the multiple-drug-resistant pheromone-responsive conjugative plasmids have been widely spread in both human and livestock VRE, and there was high potential for transfers of VRE from food animals to humans in Taiwan in the early 2000s.<\/jats:p>","DOI":"10.3390\/antibiotics12121668","type":"journal-article","created":{"date-parts":[[2023,11,27]],"date-time":"2023-11-27T11:54:48Z","timestamp":1701086088000},"page":"1668","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["High Incidence of Multiple-Drug-Resistant Pheromone-Responsive Plasmids and Transmissions of VanA-Type Vancomycin-Resistant Enterococcus faecalis between Livestock and Humans in Taiwan"],"prefix":"10.3390","volume":"12","author":[{"given":"Haruyoshi","family":"Tomita","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Gunma, Japan"},{"name":"Laboratory of Bacterial Drug Resistance, Gunma University Graduate School of Medicine, Maebashi 371-8511, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jang-Jih","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 333, Taiwan"},{"name":"Department of Medicine, College of Medicine, Chang Gung University, Taoyuan 333, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyoshi","family":"Ike","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Gunma University Graduate School of Medicine, Maebashi 371-8511, Gunma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,27]]},"reference":[{"key":"ref_1","unstructured":"Gilmore, M.S., Clewell, D.B., Ike, Y., and Shankar, N. 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