{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:18:22Z","timestamp":1791566302423,"version":"4.3.4"},"reference-count":74,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T00:00:00Z","timestamp":1703376000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"University of Geneva Institutional Funds for Senior Clinical Researchers (DM)","award":["Dnr. 4-823\/2019"],"award-info":[{"award-number":["Dnr. 4-823\/2019"]}]},{"name":"University of Geneva Institutional Funds for Senior Clinical Researchers (DM)","award":["FoUI-978687"],"award-info":[{"award-number":["FoUI-978687"]}]},{"name":"Karolinska Institutet Strategic Funds (GNB)","award":["Dnr. 4-823\/2019"],"award-info":[{"award-number":["Dnr. 4-823\/2019"]}]},{"name":"Karolinska Institutet Strategic Funds (GNB)","award":["FoUI-978687"],"award-info":[{"award-number":["FoUI-978687"]}]},{"name":"KI\/SLL Styrgruppen f\u00f6r Odontologisk Forskning (SOF)","award":["Dnr. 4-823\/2019"],"award-info":[{"award-number":["Dnr. 4-823\/2019"]}]},{"name":"KI\/SLL Styrgruppen f\u00f6r Odontologisk Forskning (SOF)","award":["FoUI-978687"],"award-info":[{"award-number":["FoUI-978687"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Enterococcus faecalis, a leading multi-resistant nosocomial pathogen, is also the most frequently retrieved species from persistently infected dental root canals, suggesting that the oral cavity is a possible reservoir for resistant strains. However, antimicrobial susceptibility testing (AST) for oral enterococci remains scarce. Here, we examined the AST profiles of 37 E. faecalis strains, including thirty-four endodontic isolates, two vanA-type vancomycin-resistant isolates, and the reference strain ATCC-29212. Using Etest gradient strips and established EUCAST standards, we determined minimum inhibitory concentrations (MICs) for amoxicillin, vancomycin, clindamycin, tigecycline, linezolid, and daptomycin. Results revealed that most endodontic isolates were susceptible to amoxicillin and vancomycin, with varying levels of intrinsic resistance to clindamycin. Isolates exceeding the clindamycin MIC of the ATCC-29212 strain were further tested against last-resort antibiotics, with 7\/27 exhibiting MICs matching the susceptibility breakpoint for tigecycline, and 1\/27 reaching that of linezolid. Both vanA isolates confirmed vancomycin resistance and demonstrated resistance to tigecycline. In conclusion, while most endodontic isolates remained susceptible to first-line antibiotics, several displayed marked intrinsic clindamycin resistance, and MICs matched tigecycline\u2019s breakpoint. The discovery of tigecycline resistance in vanA isolates highlights the propensity of clinical clone clusters to acquire multidrug resistance. Our results emphasize the importance of implementing AST strategies in dental practices for continued resistance surveillance.<\/jats:p>","DOI":"10.3390\/antibiotics13010018","type":"journal-article","created":{"date-parts":[[2023,12,24]],"date-time":"2023-12-24T20:42:30Z","timestamp":1703450550000},"page":"18","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Profiling Antibiotic Susceptibility among Distinct Enterococcus faecalis Isolates from Dental Root Canals"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9220-3977","authenticated-orcid":false,"given":"Daniel","family":"Manoil","sequence":"first","affiliation":[{"name":"Division of Cariology and Endodontics, University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland"},{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine, Karolinska Institute, Campus Huddinge, 141 52 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0715-3079","authenticated-orcid":false,"given":"Ender Efe","family":"Cerit","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine, Karolinska Institute, Campus Huddinge, 141 52 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Karolinska University Hospital Huddinge, Karolinska Institute, Campus Huddinge, 141 52 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9772-8406","authenticated-orcid":false,"given":"St\u00e9phane","family":"Durual","sequence":"additional","affiliation":[{"name":"Biomaterials Laboratory, Division of Fixed Prosthodontics and Biomaterials, University Clinics of Dental Medicine, Faculty of Medicine, University of Geneva, 1211 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8747-3307","authenticated-orcid":false,"given":"Malin","family":"Brundin","sequence":"additional","affiliation":[{"name":"Division of Endodontics, Department of Odontology, Ume\u00e5 University, 901 87 Ume\u00e5, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-0653","authenticated-orcid":false,"given":"Georgios N.","family":"Belibasakis","sequence":"additional","affiliation":[{"name":"Division of Oral Health and Periodontology, Department of Dental Medicine, Karolinska Institute, Campus Huddinge, 141 52 Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,12,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1038\/s41579-018-0107-z","article-title":"Biofilm-associated infection by enterococci","volume":"17","author":"Chong","year":"2019","journal-title":"Nat. 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