{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T20:26:11Z","timestamp":1783369571295,"version":"3.54.6"},"reference-count":53,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,5,12]],"date-time":"2024-05-12T00:00:00Z","timestamp":1715472000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"GRRC Program of Gyeonggi province","award":["GRRC-KyungHee2020(B04)"],"award-info":[{"award-number":["GRRC-KyungHee2020(B04)"]}]},{"name":"GRRC Program of Gyeonggi province","award":["2021R1A6A3A13046056"],"award-info":[{"award-number":["2021R1A6A3A13046056"]}]},{"DOI":"10.13039\/501100003725","name":"National R&amp;D Program through the National Research Foundation of Korea (NRF) funded by the Korea government (Ministry of Science and ICT)","doi-asserted-by":"publisher","award":["GRRC-KyungHee2020(B04)"],"award-info":[{"award-number":["GRRC-KyungHee2020(B04)"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National R&amp;D Program through the National Research Foundation of Korea (NRF) funded by the Korea government (Ministry of Science and ICT)","doi-asserted-by":"publisher","award":["2021R1A6A3A13046056"],"award-info":[{"award-number":["2021R1A6A3A13046056"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>(1) Background: Although Candida albicans accounts for the majority of fungal infections, therapeutic options are limited and require alternative antifungal agents with new targets; (2) Methods: A biofilm formation assay with RPMI1640 medium was performed with Liriope muscari extract. A combination antifungal assay, dimorphic transition assay, and adhesion assay were performed under the biofilm formation condition to determine the anti-biofilm formation effect. qRT-PCR analysis was accomplished to confirm changes in gene expression; (3) Results: L. muscari extract significantly reduces biofilm formation by 51.65% at 1.56 \u03bcg\/mL use and therefore increases susceptibility to miconazole. L. muscari extract also inhibited the dimorphic transition of Candida; nearly 50% of the transition was inhibited when 1.56 \u03bcg\/mL of the extract was treated. The extract of L. muscari inhibited the expression of genes related to hyphal development and extracellular matrix of 34.4% and 36.0%, respectively, as well as genes within the Ras1-cAMP-PKA, Cph2-Tec1, and MAP kinase signaling pathways of 25.58%, 7.1% and 15.8%, respectively, at 1.56 \u03bcg\/mL of L. muscari extract treatment; (4) Conclusions: L. muscari extract significantly reduced Candida biofilm formation, which lead to induced antifungal susceptibility to miconazole. It suggests that L. muscari extract is a promising anti-biofilm candidate of Candida albicans since the biofilm formation of Candida albicans is an excellent target for candidiasis regulation.<\/jats:p>","DOI":"10.3390\/antibiotics13050434","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T06:28:12Z","timestamp":1715668092000},"page":"434","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Inhibition of Candida albicans Biofilm Formation and Attenuation of Its Virulence by Liriope muscari"],"prefix":"10.3390","volume":"13","author":[{"given":"Jeonghoon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Medical Science of Meridian, College of Korean Medicine, Graduate School, Kyung Hee University, Kyungheedae-ro 6-gil, Dongdaemun-gu, Seoul 02447, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6804-745X","authenticated-orcid":false,"given":"Hyunchan","family":"Song","sequence":"additional","affiliation":[{"name":"Graduate School of Biotechnology, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si 17104, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0407-3018","authenticated-orcid":false,"given":"Kiyoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Graduate School of Biotechnology, Kyung Hee University, 1732, Deogyeong-daero, Giheung-gu, Yongin-si 17104, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/bs.apcsb.2020.12.004","article-title":"Reactive oxygen mediated apoptosis as a therapeutic approach against opportunistic Candida albicans","volume":"155","author":"Atriwal","year":"2021","journal-title":"Adv. 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