{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:08:59Z","timestamp":1790791739052,"version":"4.1.0"},"reference-count":57,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,20]],"date-time":"2024-06-20T00:00:00Z","timestamp":1718841600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>This comprehensive review aimed (1) to characterize the sub- and supragingival microbiome in patients with biofilm-induced gingivitis (including experimental gingivitis), (2) to assess its stability and evolution over time, and (3) to assess the impact of biofilm control measures on this stability. An electronic search of the MEDLINE\u00ae\/PubMed\u00ae database until December 2023 was conducted. NCBI Taxonomy, eHOMD 16S rRNA Reference Sequence, and Tree Version 15.23 databases were used to standardize taxonomic nomenclature. Out of 89 papers initially retrieved, 14 studies were finally included: 11 using experimental gingivitis as a model and three randomized clinical trials evaluating the impact of biofilm control measures. Among them, five characterized the subgingival microbiome, nine the supragingival microbiome, and one both the sub- and supragingival microbiome. In addition, five studies evaluated the effect of toothpaste, and four studies evaluated the effect of mouth rinses. The diversity and structure of the microbiome differed significantly between patients with periodontal health and those with biofilm-induced gingivitis (including experimental gingivitis). Those differences were not reversed through conventional oral hygiene measures. Specific antiseptic agents, especially if delivered as mouth rinses, may have an impact on the supra- and subgingival microbiome in gingivitis.<\/jats:p>","DOI":"10.3390\/antibiotics13060571","type":"journal-article","created":{"date-parts":[[2024,6,21]],"date-time":"2024-06-21T03:44:49Z","timestamp":1718941489000},"page":"571","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Supra- and Subgingival Microbiome in Gingivitis and Impact of Biofilm Control: A Comprehensive Review"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7557-4023","authenticated-orcid":false,"given":"Margarita","family":"Iniesta","sequence":"first","affiliation":[{"name":"ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Section of Graduate Periodontology, Department of Dental Clinic Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7522-6408","authenticated-orcid":false,"given":"Viviane","family":"Vasconcelos","sequence":"additional","affiliation":[{"name":"Section of Graduate Periodontology, Department of Dental Clinic Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Sanz","sequence":"additional","affiliation":[{"name":"ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Section of Graduate Periodontology, Department of Dental Clinic Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5554-2777","authenticated-orcid":false,"given":"David","family":"Herrera","sequence":"additional","affiliation":[{"name":"ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Section of Graduate Periodontology, Department of Dental Clinic Specialties, Faculty of Dentistry, Complutense University of Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kozak, M., and Pawlik, A. (2023). The Role of the Oral Microbiome in the Development of Diseases. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065231"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S68","DOI":"10.1111\/jcpe.12940","article-title":"Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions","volume":"45","author":"Chapple","year":"2018","journal-title":"J. Clin. Periodontol."},{"key":"ref_3","first-page":"S5","article-title":"Principles in prevention of periodontal diseases: Consensus report of group 1 of the 11th European Workshop on Periodontology on effective prevention of periodontal and peri-implant diseases","volume":"42","author":"Tonetti","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"717","DOI":"10.1038\/nrmicro2873","article-title":"The keystone-pathogen hypothesis","volume":"10","author":"Hajishengallis","year":"2012","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"5041","DOI":"10.1128\/IAI.72.9.5041-5051.2004","article-title":"Porphyromonas gingivalis lipopolysaccharide contains multiple lipid A species that functionally interact with both toll-like receptors 2 and 4","volume":"72","author":"Darveau","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"905","DOI":"10.1111\/jcpe.13793","article-title":"Subgingival microbiome in periodontal health, gingivitis and different stages of periodontitis","volume":"50","author":"Iniesta","year":"2023","journal-title":"J. Clin. Periodontol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1038\/nri3785","article-title":"Periodontitis: From microbial immune subversion to systemic inflammation","volume":"15","author":"Hajishengallis","year":"2015","journal-title":"Nat. Rev. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S6","DOI":"10.1016\/S0300-5712(10)70004-X","article-title":"Clinical efficacy of antimicrobial mouthrinses","volume":"38","author":"Gunsolley","year":"2010","journal-title":"J. Dent."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"S106","DOI":"10.1111\/jcpe.12331","article-title":"Efficacy of adjunctive anti-plaque chemical agents in managing gingivitis: A systematic review and meta-analysis","volume":"42","author":"Serrano","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1111\/idh.12617","article-title":"Effects of antimicrobial mouthwashes on the human oral microbiome: Systematic review of controlled clinical trials","volume":"21","author":"Hassan","year":"2023","journal-title":"Int. J. Dent. Hyg."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1111\/prd.12362","article-title":"Microbial signatures of health, gingivitis, and periodontitis","volume":"86","author":"Abusleme","year":"2021","journal-title":"Periodontol. 2000"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1177\/0022034516668847","article-title":"Clinical, Immune, and Microbiome Traits of Gingivitis and Peri-implant Mucositis","volume":"96","author":"Schincaglia","year":"2017","journal-title":"J. Dent. Res."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e2012578118","DOI":"10.1073\/pnas.2012578118","article-title":"Human variation in gingival inflammation","volume":"118","author":"Bamashmous","year":"2021","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1038\/s43705-023-00229-5","article-title":"Suspension of oral hygiene practices highlights key bacterial shifts in saliva, tongue, and tooth plaque during gingival inflammation and resolution","volume":"3","author":"Hall","year":"2023","journal-title":"ISME Commun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1002\/JPER.22-0710","article-title":"Effects of a stabilized stannous fluoride dentifrice on clinical, immunomodulatory, and microbial outcomes in a human experimental gingivitis model","volume":"95","author":"Fine","year":"2023","journal-title":"J. Periodontol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1768","DOI":"10.1038\/ismej.2014.32","article-title":"Predictive modeling of gingivitis severity and susceptibility via oral microbiota","volume":"8","author":"Huang","year":"2014","journal-title":"ISME J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1038\/ijos.2016.18","article-title":"Cetylpyridinium chloride mouth rinses alleviate experimental gingivitis by inhibiting dental plaque maturation","volume":"8","author":"Teng","year":"2016","journal-title":"Int. J. Oral Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Nowicki, E.M., Shroff, R., Singleton, J.A., Renaud, D.E., Wallace, D., Drury, J., Zirnheld, J., Colleti, B., Ellington, A.D., and Lamont, R.J. (2018). Microbiota and Metatranscriptome Changes Accompanying the Onset of Gingivitis. mBio, 9.","DOI":"10.1128\/mBio.00575-18"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Huang, S., He, T., Yue, F., Xu, X., Wang, L., Zhu, P., Teng, F., Sun, Z., Liu, X., and Jing, G. (2021). Longitudinal Multi-omics and Microbiome Meta-analysis Identify an Asymptomatic Gingival State That Links Gingivitis, Periodontitis, and Aging. mBio, 12.","DOI":"10.1128\/mBio.03281-20"},{"key":"ref_20","first-page":"57","article-title":"Impact of Oral Hygiene Discontinuation on Supragingival and Salivary Microbiomes","volume":"3","author":"Grande","year":"2018","journal-title":"JDR Clin. Trans. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Al-Kamel, A., Baraniya, D., Al-Hajj, W.A., Halboub, E., Abdulrab, S., Chen, T., and Al-Hebshi, N.N. (2019). Subgingival microbiome of experimental gingivitis: Shifts associated with the use of chlorhexidine and N-acetyl cysteine mouthwashes. J. Oral Microbiol., 11.","DOI":"10.1080\/20002297.2019.1608141"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Kistler, J.O., Booth, V., Bradshaw, D.J., and Wade, W.G. (2013). Bacterial community development in experimental gingivitis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071227"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"24705","DOI":"10.1038\/srep24705","article-title":"Microbiota-based Signature of Gingivitis Treatments: A Randomized Study","volume":"6","author":"Huang","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Keijser, B.J.F., van den Broek, T.J., Slot, D.E., van Twillert, L., Kool, J., Thabuis, C., Ossendrijver, M., van der Weijden, F.A., and Montijn, R.C. (2018). The Impact of Maltitol-Sweetened Chewing Gum on the Dental Plaque Biofilm Microbiota Composition. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.00381"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Newman, B.A., Rosebrough, C.N., Tamashiro, R.A., Dias Ribeiro, A.P., Whitlock, J.A., Sidhu, G., Aukhil, I., Porral, D.Y., Progulske-Fox, A., and Myntti, M.F. (2022). A randomized controlled trial to evaluate the effectiveness of a novel mouth rinse in patients with gingivitis. BMC Oral Health, 22.","DOI":"10.1186\/s12903-022-02518-2"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1038\/nature14486","article-title":"Unusual biology across a group comprising more than 15% of domain Bacteria","volume":"523","author":"Brown","year":"2015","journal-title":"Nature"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.syapm.2013.09.007","article-title":"Multilocus analysis reveals diversity in the genus Tissierella: Description of Tissierella carlieri sp. nov. in the new class Tissierellia classis nov","volume":"37","author":"Alauzet","year":"2014","journal-title":"Syst. Appl. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J. Bacteriol."},{"key":"ref_29","unstructured":"The Forsyth Institute (2024, March 23). Genus: Haemophilus. Available online: https:\/\/www.homd.org\/taxa\/ecology?rank=genus&name=Haemophilus."},{"key":"ref_30","unstructured":"The Forsyth Institute (2024, April 10). Expanded Human Oral Microbiome Database (eHOMD). Available online: https:\/\/www.homd.org\/."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Bhandary, R., Venugopalan, G., Ramesh, A., Tartaglia, G.M., Singhal, I., and Khijmatgar, S. (2024). Microbial Symphony: Navigating the Intricacies of the Human Oral Microbiome and Its Impact on Health. Microorganisms, 12.","DOI":"10.3390\/microorganisms12030571"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.chom.2017.08.013","article-title":"Host Genetic Control of the Oral Microbiome in Health and Disease","volume":"22","author":"Gomez","year":"2017","journal-title":"Cell Host Microbe"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Mukherjee, C., Moyer, C.O., Steinkamp, H.M., Hashmi, S.B., Beall, C.J., Guo, X., Ni, A., Leys, E.J., and Griffen, A.L. (2021). Acquisition of oral microbiota is driven by environment, not host genetics. Microbiome, 9.","DOI":"10.1186\/s40168-020-00986-8"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"15779","DOI":"10.1038\/s41598-019-52115-7","article-title":"Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells","volume":"9","author":"Brown","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Szafra\u0144ski, S.P., Deng, Z.L., Tomasch, J., Jarek, M., Bhuju, S., Meisinger, C., K\u00fchnisch, J., Sztajer, H., and Wagner-D\u00f6bler, I. (2015). Functional biomarkers for chronic periodontitis and insights into the roles of Prevotella nigrescens and Fusobacterium nucleatum; a metatranscriptome analysis. NPJ Biofilms Microbiomes, 1.","DOI":"10.1038\/npjbiofilms.2015.17"},{"key":"ref_36","first-page":"S1","article-title":"Primary and secondary prevention of periodontal and peri-implant diseases: Introduction to, and objectives of the 11th European Workshop on Periodontology consensus conference","volume":"42","author":"Tonetti","year":"2015","journal-title":"J. Clin. Periodontol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1038\/ismej.2014.114","article-title":"The subgingival microbiome of clinically healthy current and never smokers","volume":"9","author":"Mason","year":"2015","journal-title":"ISME J."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2435","DOI":"10.1038\/ismej.2016.37","article-title":"Cigarette smoking and the oral microbiome in a large study of American adults","volume":"10","author":"Wu","year":"2016","journal-title":"ISME J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Mazurel, D., Carda-Di\u00e9guez, M., Langenburg, T., \u017diemyt\u0117, M., Johnston, W., Mart\u00ednez, C.P., Albalat, F., Llena, C., Al-Hebshi, N., and Culshaw, S. (2023). Nitrate and a nitrate-reducing Rothia aeria strain as potential prebiotic or synbiotic treatments for periodontitis. NPJ Biofilms Microbiomes, 9.","DOI":"10.1038\/s41522-023-00406-3"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"7067","DOI":"10.1128\/JB.182.24.7067-7069.2000","article-title":"Intergeneric communication in dental plaque biofilms","volume":"182","author":"Xie","year":"2000","journal-title":"J. Bacteriol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"16217","DOI":"10.1038\/s41598-017-16522-y","article-title":"A novel peptidic inhibitor derived from Streptococcus cristatus ArcA attenuates virulence potential of Porphyromonas gingivalis","volume":"7","author":"Ho","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"3902","DOI":"10.1128\/JCM.00072-09","article-title":"Negative correlation of distributions of Streptococcus cristatus and Porphyromonas gingivalis in subgingival plaque","volume":"47","author":"Wang","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Uranga, C.C., Arroyo, P., Duggan, B.M., Gerwick, W.H., and Edlund, A. (2020). Commensal Oral Rothia mucilaginosa Produces Enterobactin, a Metal-Chelating Siderophore. mSystems, 5.","DOI":"10.1128\/mSystems.00161-20"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Huang, S., Haiminen, N., Carrieri, A.P., Hu, R., Jiang, L., Parida, L., Russell, B., Allaband, C., Zarrinpar, A., and V\u00e1zquez-Baeza, Y. (2020). Human Skin, Oral, and Gut Microbiomes Predict Chronological Age. mSystems, 5.","DOI":"10.1128\/mSystems.00630-19"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1536","DOI":"10.1002\/JPER.20-0778","article-title":"Nitrate-rich diet alters the composition of the oral microbiota in periodontal recall patients","volume":"92","author":"Schlagenhauf","year":"2021","journal-title":"J. Periodontol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/j.archoralbio.2018.02.010","article-title":"Effect of sodium fluoride on oral biofilm microbiota and enamel demineralization","volume":"89","author":"Thurnheer","year":"2018","journal-title":"Arch. Oral Biol."},{"key":"ref_47","first-page":"S1","article-title":"Advanced oral antibacterial\/anti-inflammatory technology: A comprehensive review of the clinical benefits of a triclosan\/copolymer\/fluoride dentifrice","volume":"16","author":"Panagakos","year":"2005","journal-title":"J. Clin. Dent."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"4770","DOI":"10.1128\/AEM.69.8.4770-4776.2003","article-title":"Effects of a chlorhexidine gluconate-containing mouthwash on the vitality and antimicrobial susceptibility of in vitro oral bacterial ecosystems","volume":"69","author":"McBain","year":"2003","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1421","DOI":"10.1902\/jop.2009.090185","article-title":"Comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray","volume":"80","author":"Colombo","year":"2009","journal-title":"J. Periodontol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1038\/ismej.2017.119","article-title":"Exact sequence variants should replace operational taxonomic units in marker-gene data analysis","volume":"11","author":"Callahan","year":"2017","journal-title":"ISME J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"16321","DOI":"10.1038\/s41598-018-34294-x","article-title":"Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling","volume":"8","author":"Teng","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Gloor, G.B., Macklaim, J.M., Pawlowsky-Glahn, V., and Egozcue, J.J. (2017). Microbiome Datasets Are Compositional: And This Is Not Optional. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.02224"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"210","DOI":"10.1093\/bib\/bbx104","article-title":"A broken promise: Microbiome differential abundance methods do not control the false discovery rate","volume":"20","author":"Hawinkel","year":"2019","journal-title":"Brief. Bioinform."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1111\/j.1600-051X.2007.01170.x","article-title":"Subgingival microbial profiles in chronic periodontitis patients from Chile, Colombia and Spain","volume":"35","author":"Herrera","year":"2008","journal-title":"J. Clin. Periodontol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1111\/1348-0421.12759","article-title":"Measuring the subgingival microbiota in periodontitis patients: Comparison of the surface layer and the underlying layers","volume":"64","author":"Liu","year":"2020","journal-title":"Microbiol. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Oren, A., and Garrity, G.M. (2021). Valid publication of the names of forty-two phyla of prokaryotes. Int. J. Syst. Evol. Microbiol., 71.","DOI":"10.1099\/ijsem.0.005056"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1099\/ijsem.0.002593","article-title":"Proposal of the suffix-ota to denote phyla. Addendum to \u2018Proposal to include the rank of phylum in the International Code of Nomenclature of Prokaryotes\u2019","volume":"68","author":"Whitman","year":"2018","journal-title":"Int. J. Syst. Evol. Microbiol."}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/13\/6\/571\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:01:56Z","timestamp":1760108516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/13\/6\/571"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,20]]},"references-count":57,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["antibiotics13060571"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics13060571","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,20]]}}}