{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T08:39:57Z","timestamp":1782549597143,"version":"3.54.5"},"reference-count":78,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T00:00:00Z","timestamp":1719014400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science Fund of the Republic of Serbia","award":["6066966"],"award-info":[{"award-number":["6066966"]}]},{"name":"Science Fund of the Republic of Serbia","award":["451-03-47\/2023-01\/200117"],"award-info":[{"award-number":["451-03-47\/2023-01\/200117"]}]},{"name":"PROMIS","award":["6066966"],"award-info":[{"award-number":["6066966"]}]},{"name":"PROMIS","award":["451-03-47\/2023-01\/200117"],"award-info":[{"award-number":["451-03-47\/2023-01\/200117"]}]},{"name":"InfoBomat","award":["6066966"],"award-info":[{"award-number":["6066966"]}]},{"name":"InfoBomat","award":["451-03-47\/2023-01\/200117"],"award-info":[{"award-number":["451-03-47\/2023-01\/200117"]}]},{"name":"Ministry of Science and Technological Development, Republic of Serbia","award":["6066966"],"award-info":[{"award-number":["6066966"]}]},{"name":"Ministry of Science and Technological Development, Republic of Serbia","award":["451-03-47\/2023-01\/200117"],"award-info":[{"award-number":["451-03-47\/2023-01\/200117"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Bovine mastitis, as a significant and prevalent health problem in dairy herds, is primarily attributed to bacterial infections. Yeasts, although considered relatively rare causative agents, have also been associated with mastitis in dairy cattle. Current mastitis treatment predominantly relies on antibiotics, with limited emphasis on antifungal treatment. However, mycotic mastitis is challenging to treat, since these fungi are often resistant to antibiotics and may even utilize them for energy. In the current research, the in vivo antimicrobial activity of the essential oil-based formulation (Phyto-Bomat), as a possible alternative mastitis treatment associated with yeasts, was studied. This study involved a total of 68 animals from two dairy farms with diagnosed mastitis, and three treatment groups were established: conventional antibiotic treatment, Phyto-Bomat treatment, and the combination of both. The findings suggest significant variations in the presence of Candida samples based on the treatment administered, and the most significant difference was noted in cows treated with the combination (Phyto-Bomat and antibiotics). Yet, it is important to note that the results reveal that, regardless of the treatment type, there are statistically significant differences in the presence of Candida samples across the examined time points. These results aim to provide valuable insights into the potential of EOs as an alternative therapy in bovine mastitis, specifically targeting yeasts. Such findings could offer new strategies in the mycotic mastitis control and reducing the occurrence of secondary infections following antibiotic treatment.<\/jats:p>","DOI":"10.3390\/antibiotics13070575","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T08:42:03Z","timestamp":1719218523000},"page":"575","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Clinical Evidence on Expansion of Essential Oil-Based Formulation\u2019s Pharmacological Activity in Bovine Mastitis Treatment: Antifungal Potential as Added Value"],"prefix":"10.3390","volume":"13","author":[{"given":"Dragana","family":"Tomani\u0107","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5373-5540","authenticated-orcid":false,"given":"Dragana D.","family":"Bo\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11221 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5781-6412","authenticated-orcid":false,"given":"Neboj\u0161a","family":"Kladar","sequence":"additional","affiliation":[{"name":"Center for Medical and Pharmaceutical Investigations and Quality Control, Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"},{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0402-3173","authenticated-orcid":false,"given":"Marko","family":"Samard\u017eija","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Api\u0107","sequence":"additional","affiliation":[{"name":"Scientific Veterinary Institute Novi Sad, Rumena\u010dki put 20, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3269-5815","authenticated-orcid":false,"given":"Jovan","family":"Baljak","sequence":"additional","affiliation":[{"name":"Center for Medical and Pharmaceutical Investigations and Quality Control, Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"},{"name":"Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0133-872X","authenticated-orcid":false,"given":"Zorana","family":"Kova\u010devi\u0107","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Trg Dositeja Obradovica 8, 21000 Novi Sad, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,22]]},"reference":[{"key":"ref_1","first-page":"3","article-title":"Bovine mycotic mastitis","volume":"47","author":"Krukowski","year":"2003","journal-title":"Folia Vet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.24099\/vet.arhiv.0116","article-title":"Bovine mastitis: A persistent and evolving problem requiring novel approaches for its control-a review","volume":"88","author":"Habrun","year":"2018","journal-title":"Vet. 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