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guanidine core has been one of the most studied functional groups in medicinal chemistry, and guanylation reactions are powerful tools for synthesizing this kind of compound. In this study, a series of five guanidine-core small molecules were obtained through guanylation reactions. These compounds were then evaluated against three different strains of Escherichia coli, one collection strain from the American Type Culture Collection (ATCC) of E. coli ATCC 35218, and two clinical extended-spectrum beta-lactamase (ESBL)-producing E. coli isolates (ESBL1 and ESBL2). Moreover, three different strains of Pseudomonas aeruginosa were studied, one collection strain of P. aeruginosa ATCC 27853, and two clinical multidrug-resistant isolates (PA24 and PA35). Among Gram-positive strains, three different strains of Staphylococcus aureus, one collection strain of S. aureus ATCC 29213, and two clinical methicillin-resistant S. aureus (MRSA1 and MRSA2) were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) experiments were reported, and the drop plate (DP) method was used to determine the number of viable suspended bacteria in a known beaker volume. The results from this assessment suggest that guanidine-core small molecules hold promise as therapeutic alternatives for treating infections caused by clinical Gram-negative and Gram-positive bacteria, highlighting the need for further studies to explore their potential. The results from this assessment suggest that the chemical structure of CAPP4 might serve as the basis for designing more active guanidine-based antimicrobial compounds, highlighting the need for further studies to explore their potential.<\/jats:p>","DOI":"10.3390\/antibiotics13070609","type":"journal-article","created":{"date-parts":[[2024,7,2]],"date-time":"2024-07-02T11:33:53Z","timestamp":1719920033000},"page":"609","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A New Guanidine-Core Small-Molecule Compound as a Potential Antimicrobial Agent against Resistant Bacterial Strains"],"prefix":"10.3390","volume":"13","author":[{"given":"Noelia","family":"Morata-Moreno","sequence":"first","affiliation":[{"name":"Department of Otorrinolaringology, Complejo Hospitalario Universitario, 02006 Albacete, 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Mol. Evol."},{"key":"ref_2","first-page":"134","article-title":"Novel drug candidates against antibiotic-resistant microorganisms: A review","volume":"27","author":"Lim","year":"2024","journal-title":"Iran. J. Basic Med. Sci."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Serweci\u0144ska, L. (2020). Antimicrobials and Antibiotic-Resistant Bacteria: A Risk to the Environment and to Public Health. Water, 12.","DOI":"10.3390\/w12123313"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4221","DOI":"10.1016\/j.bmcl.2017.08.027","article-title":"Antibiotic adjuvants\u2014A strategy to unlock bacterial resistance to antibiotics","volume":"27","year":"2017","journal-title":"Bioorg. Med. Chem. Lett."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1016\/j.cell.2007.03.004","article-title":"Molecular mechanisms of antibacterial multidrug resistance","volume":"128","author":"Alekshun","year":"2007","journal-title":"Cell"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Browne, K., Chakraborty, S., Chen, R., Willcox, M.D., Black, D.S., Walsh, W.R., and Kumar, N. (2020). A New Era of Antibiotics: The Clinical Potential of Antimicrobial Peptides. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21197047"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.phrs.2016.02.012","article-title":"Rosuvastatin: Beyond the cholesterol-lowering effect","volume":"107","author":"Cortese","year":"2016","journal-title":"Pharmacol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1210\/endrev\/bnaa023","article-title":"Cellular and Molecular Mechanisms of Metformin Action","volume":"42","author":"LaMoia","year":"2021","journal-title":"Endocr. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.2174\/1381612823666170316124500","article-title":"Glial Cell: A Potential Target for Cellular and Drug Based Therapy in Various CNS Diseases","volume":"23","author":"Ahmed","year":"2017","journal-title":"Curr. Pharm. Des."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1016\/j.jbspin.2018.07.004","article-title":"Methotrexate mechanism in treatment of rheumatoid arthritis","volume":"86","author":"Friedman","year":"2019","journal-title":"Jt. Bone Spine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"113293","DOI":"10.1016\/j.ejmech.2021.113293","article-title":"Antimicrobial drugs bearing guanidine moieties: A review","volume":"216","author":"Kim","year":"2021","journal-title":"Eur. J. Med. Chem."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"3406","DOI":"10.1039\/C4CS00013G","article-title":"Guanidines: From classical approaches to efficient catalytic syntheses","volume":"43","author":"Otero","year":"2014","journal-title":"Chem. Soc. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Del Campo-Balguer\u00edas, A., Parra-Cadenas, B., Nieto-Jimenez, C., Bravo, I., Ripoll, C., Poyatos-Racionero, E., Gancarski, P., Carrillo-Hermosilla, F., Alonso-Moreno, C., and Oca\u00f1a, A. (2023). Guanylation Reactions for the Rational Design of Cancer Therapeutic Agents. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms241813820"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2789","DOI":"10.1021\/om1003122","article-title":"Simple, Versatile, and Efficient Catalysts for Guanylation of Amines","volume":"29","author":"Otero","year":"2010","journal-title":"Organometallics"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Nam-Cha, S.H., Dom\u00ednguez-Jurado, E., Tinoco-Valencia, S.L., P\u00e9rez-Tanoira, R., Morata-Moreno, N., Alfaro-Ruiza, R., Lara-S\u00e1nchez, A., Esteban, J., Luj\u00e1n, R., and Alonso-Moreno, C. (2023). Synthesis, characterization, and antibacterial activities of a heteroscorpionate derivative platinum complex against methicillin-resistant Staphylococcus aureus. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1100947"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Hernando-Gozalo, M., Aguilera-Correa, J.J., Rescalvo-Casas, C., Seijas-Pereda, L., Garc\u00eda-Bertol\u00edn, C., de la Mata, F.J., S\u00e1nchez-Nieves, J., Cuadros, J., and P\u00e9rez-Tanoira, R. (2023). Study of the antimicrobial activity of cationic carbosilane dendrimers against clinical strains of multidrug-resistant bacteria and their biofilms. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1203991"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e01934-17","DOI":"10.1128\/JCM.01934-17","article-title":"CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial Susceptibility Tests","volume":"56","author":"Humphries","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"16306","DOI":"10.1038\/s41598-021-95609-z","article-title":"A novel bis(pyrazolyl)methane compound as a potential agent against Gram-positive bacteria","volume":"11","author":"Esteban","year":"2021","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/S0167-7012(00)00241-4","article-title":"How to optimize the drop plate method for enumerating bacteria","volume":"44","author":"Herigstad","year":"2001","journal-title":"J. Microbiol. Methods"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Vitt, A., Sofrata, A., Slizen, V., Sugars, R.V., Gustafsson, A., Gudkova, E.I., Kazeko, L.A., Ramberg, P., and Buhlin, K. (2015). Antimicrobial activity of polyhexamethylene guanidine phosphate in comparison to chlorhexidine using the quantitative suspension method. Ann. Clin. Microbiol. Antimicrob., 14.","DOI":"10.1186\/s12941-015-0097-x"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.mimet.2010.02.004","article-title":"Evaluation of diffusion and dilution methods to determine the antibacterial activity of plant extracts","volume":"81","author":"Klancnik","year":"2010","journal-title":"J. Microbiol. Methods"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Gomes, A.R., Varela, C.L., Pires, A.S., Tavares-da-Silva, E.J., and Roleira, F.M.F. (2023). Synthetic and natural guanidine derivatives as antitumor and antimicrobial agents: A review. Bioorg. Chem., 138.","DOI":"10.1016\/j.bioorg.2023.106600"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/j.cbi.2017.12.009","article-title":"Aminoguanidine hydrazones (AGH\u2019s) as modulators of norfloxacin resistance in Staphylococcus aureus that overexpress NorA efflux pump","volume":"280","author":"Dantas","year":"2018","journal-title":"Chem. Biol. Interact."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"354","DOI":"10.1080\/14756366.2019.1702654","article-title":"Synthesis, antibacterial and anticancer activity, and docking study of aminoguanidines containing an alkynyl moiety","volume":"35","author":"Deng","year":"2020","journal-title":"J. Enzyme. Inhib. Med. Chem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5860","DOI":"10.1128\/AAC.01016-13","article-title":"An FtsZ-targeting prodrug with oral antistaphylococcal efficacy in vivo","volume":"57","author":"Kaul","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"10633","DOI":"10.1021\/acsomega.0c00830","article-title":"Contribution of Organofluorine Compounds to Pharmaceuticals","volume":"5","author":"Inoue","year":"2020","journal-title":"ACS Omega"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"8251","DOI":"10.1038\/s41598-017-08749-6","article-title":"Identification, synthesis and biological activity of alkyl-guanidine oligomers as potent antibacterial agents","volume":"7","author":"Zamperini","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1363","DOI":"10.1016\/j.bmcl.2019.03.040","article-title":"Synthesis and characterization of polyaniline-drug conjugates as effective antituberculosis agents","volume":"29","author":"Kashyap","year":"2019","journal-title":"Bioorg. Med. Chem. 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