{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:52:55Z","timestamp":1778561575352,"version":"3.51.4"},"reference-count":60,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T00:00:00Z","timestamp":1722038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical University of Bialystok","award":["N\/ST\/ZB\/16\/005\/1164"],"award-info":[{"award-number":["N\/ST\/ZB\/16\/005\/1164"]}]},{"name":"Medical University of Bialystok","award":["N\/ST\/ZB\/17\/002\/1164"],"award-info":[{"award-number":["N\/ST\/ZB\/17\/002\/1164"]}]},{"name":"BTL Polska sp.z.o.o.","award":["N\/ST\/ZB\/16\/005\/1164"],"award-info":[{"award-number":["N\/ST\/ZB\/16\/005\/1164"]}]},{"name":"BTL Polska sp.z.o.o.","award":["N\/ST\/ZB\/17\/002\/1164"],"award-info":[{"award-number":["N\/ST\/ZB\/17\/002\/1164"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: This study aimed to evaluate alterations in the concentrations of matrix metalloproteinase-9 (MMP-9) and interleukin-8 (IL-8) within gingival crevicular fluid (GCF) extracted from the intrabony periodontal defect site before and after minimally invasive regenerative surgery, with or without supplemental laser application. The surgical procedure was performed using the modified minimally invasive surgical technique (M-MIST). Methods: Thirty-eight patients, each presenting with a single vertical defect, were randomly assigned to either the test (M-MIST + Er:YAG + Nd:YAG) or the control group (M-MIST). IL-8 and MMP-9 levels (primary outcomes of the study) were assessed prior to therapy, after 2 and 4 weeks, and 6 months following the surgical procedure by means of dedicated ELISA kits. Results: Both procedures were clinically effective as evidenced by probing depth (PD) reduction and clinical attachment level (CAL) gain at the 6-month follow-up. No statistical differences were observed in the levels of MMP-9 and IL-8 between the groups at any time point assessed. The changes in the level of MMP-9 and IL-8 over time were not statistically significant in any group. IL-8 was positively correlated with MMP-9 in the control group throughout the study and in the test group 2 weeks and 6 months post-op. Conclusions: Within the limitations of this study, the additional application of Er:YAG + Nd:YAG lasers alongside the M-MIST procedure did not enhance the clinical and biochemical treatment outcomes compared to M-MIST alone.<\/jats:p>","DOI":"10.3390\/antibiotics13080704","type":"journal-article","created":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T11:02:27Z","timestamp":1722337347000},"page":"704","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Matrix Metalloproteinase 9 (MMP-9) and Interleukin-8 (IL-8) in Gingival Crevicular Fluid after Minimally Invasive Periodontal Surgery with or without Er:YAG and Nd:YAG Laser Application"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3122-4990","authenticated-orcid":false,"given":"Ewa","family":"Doli\u0144ska","sequence":"first","affiliation":[{"name":"Department of Periodontal and Oral Mucosa Diseases, Medical University of Bialystok, ul. Waszyngtona 13, 15-269 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7473-3552","authenticated-orcid":false,"given":"Anna","family":"Skurska","sequence":"additional","affiliation":[{"name":"Department of Periodontal and Oral Mucosa Diseases, Medical University of Bialystok, ul. Waszyngtona 13, 15-269 Bialystok, Poland"},{"name":"Department of Integrated Dentistry, Medical University of Bialystok, ul. M. Sk\u0142odowskiej-Curie 24A, 15-276 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Violetta","family":"Dymicka-Piekarska","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, ul. Waszyngtona 15, 15-269 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6241-8283","authenticated-orcid":false,"given":"Robert","family":"Milewski","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Medical Informatics, Medical University of Bialystok, ul. Szpitalna 37, 15-295 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u0142gorzata","family":"Pietruska","sequence":"additional","affiliation":[{"name":"Department of Periodontal and Oral Mucosa Diseases, Medical University of Bialystok, ul. Waszyngtona 13, 15-269 Bialystok, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S74","DOI":"10.1002\/JPER.17-0719","article-title":"Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions","volume":"89","author":"Chapple","year":"2018","journal-title":"J. Periodontol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"S130","DOI":"10.1111\/jcpe.12944","article-title":"Age-dependent distribution of periodontitis in two countries:findings from NHANES 2009 to 2014 and SHIP-TREND 2008 to 2012","volume":"45","author":"Billings","year":"2018","journal-title":"J. Clin. 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