{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:50:04Z","timestamp":1789519804170,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,3]],"date-time":"2024-08-03T00:00:00Z","timestamp":1722643200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Clostridioides difficile is a widely distributed anaerobic pathogen. C. difficile infection is a serious problem in healthcare. Its biofilms have been found to exhibit biocorrosivity, albeit very little, but sufficient for it to correlate with biofilm growth\/health. This work demonstrated the use of a disposable electrochemical biofilm test kit using two solid-state electrodes (a 304 stainless steel working electrode, and a graphite counter electrode, which also served as the reference electrode) in a 10 mL serum vial. It was found that the C. difficile 630\u2206erm Adp-4 mutant had a minimum inhibitory concentration (MIC) for vancomycin twice that of the 630\u2206erm wild type strain in biofilm prevention (2 ppm vs. 1 ppm by mass) on 304 stainless steel. Glutaraldehyde, a commonly used hospital disinfectant, was found ineffective at 2% (w\/w) for the prevention of C. difficile 630\u2206erm wild type biofilm formation, while tetrakis(hydroxymethyl)phosphonium sulfate (THPS) disinfectant was very effective at 100 ppm for both biofilm prevention and biofilm killing. These antimicrobial efficacy data were consistent with sessile cell count and biofilm imaging results. Furthermore, the test kit provided additional transient biocide treatment information. It showed that vancomycin killed C. difficile 630\u2206erm wild type biofilms in 2 d, while THPS only required minutes.<\/jats:p>","DOI":"10.3390\/antibiotics13080728","type":"journal-article","created":{"date-parts":[[2024,8,5]],"date-time":"2024-08-05T15:45:22Z","timestamp":1722872722000},"page":"728","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A New Convenient Method to Assess Antibiotic Resistance and Antimicrobial Efficacy against Pathogenic Clostridioides difficile Biofilms"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6556-6633","authenticated-orcid":false,"given":"Lingjun","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Chemical & Biomolecular Engineering, Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bijay","family":"Gurung","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ohio University Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45071, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chris","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ohio University Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45071, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8548-1602","authenticated-orcid":false,"given":"Shaohua","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Ohio University Heritage College of Osteopathic Medicine, Ohio University, Athens, OH 45071, USA"},{"name":"Infectious and Tropical Disease Institute, Ohio University, Athens, OH 45071, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingyue","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Chemical & Biomolecular Engineering, Edison Biotechnology Institute, Ohio University, Athens, OH 45701, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kiersnowska, Z.M., Lemiech-Mirowska, E., Micha\u0142kiewicz, M., Sierocka, A., and Marczak, M. 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