{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T00:58:36Z","timestamp":1793149116831,"version":"4.5.0"},"reference-count":62,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,8,23]],"date-time":"2024-08-23T00:00:00Z","timestamp":1724371200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Guangxi BaGui Scholars Program Foundation","award":["2019A50"],"award-info":[{"award-number":["2019A50"]}]},{"name":"the Guangxi BaGui Scholars Program Foundation","award":["32202850"],"award-info":[{"award-number":["32202850"]}]},{"name":"the Guangxi BaGui Scholars Program Foundation","award":["2021GXNSFAA196004"],"award-info":[{"award-number":["2021GXNSFAA196004"]}]},{"name":"the Guangxi BaGui Scholars Program Foundation","award":["2024BZRC013"],"award-info":[{"award-number":["2024BZRC013"]}]},{"name":"the National Natural Science Foundation of China","award":["2019A50"],"award-info":[{"award-number":["2019A50"]}]},{"name":"the National Natural Science Foundation of China","award":["32202850"],"award-info":[{"award-number":["32202850"]}]},{"name":"the National Natural Science Foundation of China","award":["2021GXNSFAA196004"],"award-info":[{"award-number":["2021GXNSFAA196004"]}]},{"name":"the National Natural Science Foundation of China","award":["2024BZRC013"],"award-info":[{"award-number":["2024BZRC013"]}]},{"name":"Guangxi Natural Science Foundation","award":["2019A50"],"award-info":[{"award-number":["2019A50"]}]},{"name":"Guangxi Natural Science Foundation","award":["32202850"],"award-info":[{"award-number":["32202850"]}]},{"name":"Guangxi Natural Science Foundation","award":["2021GXNSFAA196004"],"award-info":[{"award-number":["2021GXNSFAA196004"]}]},{"name":"Guangxi Natural Science Foundation","award":["2024BZRC013"],"award-info":[{"award-number":["2024BZRC013"]}]},{"name":"Guangxi University Natural Science and Technology Innovation Development Multiplier Program Project","award":["2019A50"],"award-info":[{"award-number":["2019A50"]}]},{"name":"Guangxi University Natural Science and Technology Innovation Development Multiplier Program Project","award":["32202850"],"award-info":[{"award-number":["32202850"]}]},{"name":"Guangxi University Natural Science and Technology Innovation Development Multiplier Program Project","award":["2021GXNSFAA196004"],"award-info":[{"award-number":["2021GXNSFAA196004"]}]},{"name":"Guangxi University Natural Science and Technology Innovation Development Multiplier Program Project","award":["2024BZRC013"],"award-info":[{"award-number":["2024BZRC013"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Necrotic enteritis (NE) is a critical disease affecting broiler health, with Clostridium perfringens as its primary pathogen. Polygonum hydropiper compound extract (PHCE), formulated based on traditional Chinese veterinary principles, contains primarily flavonoids with antibacterial, anti-inflammatory, and antioxidant properties. However, PHCE\u2019s efficacy against Clostridium perfringens-induced NE and its underlying mechanism remain unclear. This study employed network pharmacology and molecular docking to predict PHCE\u2019s potential mechanisms in treating NE, followed by determining its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Clostridium perfringens (C. perf). Subsequently, the effects of various PHCE doses on intestinal damage, antioxidant capacity, and inflammatory factors in C. perf-infected broilers were assessed. Network pharmacology and molecular docking suggested that PHCE\u2019s therapeutic mechanism for NE involves the NOD-like receptor thermal protein domain associated protein 3 (NLRP3) inflammasome signaling pathway, with flavonoids such as quercetin, kaempferol, and isorhamnetin as key active components. PHCE exhibited an MIC of 3.13 mg\/mL and an MBC of 12.5 mg\/mL against C. perf. High PHCE doses effectively reduced intestinal damage scores in both the jejunum and ileum, accompanied by attenuated intestinal pathological changes. Additionally, the high dose significantly increased superoxide dismutase (SOD) levels while decreasing malondialdehyde (MDA), hydrogen peroxide (H2O2), tumor necrosis factor-alpha (TNF-\u03b1), interleukin-1 beta (IL-1\u03b2), and interleukin-6 (IL-6) in the jejunum and ileum (p &lt; 0.01 or p &lt; 0.05). PHCE also modulated the expression of caspase-1, IL-1\u03b2, gasdermin D (GSDMD), and NLRP3 mRNA, key components of the NLRP3 inflammasome signaling pathway, in both intestinal segments. These findings collectively indicate that PHCE protects against C. perf-induced oxidative stress and inflammatory damage in NE. By enhancing antioxidant capacity, PHCE likely reduces oxidative stress and inflammatory responses, subsequently modulating NLRP3 inflammasome signaling pathway key factor expression. Overall, this research provides valuable insights into the protective mechanism of the herbal compound PHCE and its potential benefits for avian health.<\/jats:p>","DOI":"10.3390\/antibiotics13090793","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T04:48:25Z","timestamp":1724647705000},"page":"793","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Polygonum hydropiper Compound Extract Inhibits Clostridium perfringens-Induced Intestinal Inflammatory Response and Injury in Broiler Chickens by Modulating NLRP3 Inflammasome Signaling"],"prefix":"10.3390","volume":"13","author":[{"given":"Jinwu","family":"Zhang","sequence":"first","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunzi","family":"Peng","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maojie","family":"Lv","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shisen","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liji","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxun","family":"Feng","sequence":"additional","affiliation":[{"name":"College of Life Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingyi","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingjun","family":"Hu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6204-1826","authenticated-orcid":false,"given":"Jiakang","family":"He","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1924-9952","authenticated-orcid":false,"given":"Zhixun","family":"Xie","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9517-9802","authenticated-orcid":false,"given":"Meiling","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning 530004, China"},{"name":"Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1080\/21505594.2021.1886777","article-title":"Pathogenicity and virulence of Clostridium perfringens","volume":"12","author":"Navarro","year":"2021","journal-title":"Virulence"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"195","DOI":"10.1016\/S0168-1605(01)00680-8","article-title":"Clostridium perfringens and foodborne infections","volume":"74","author":"Brynestad","year":"2002","journal-title":"Int. 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