{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T22:32:06Z","timestamp":1787869926352,"version":"build-2784847793"},"reference-count":87,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T00:00:00Z","timestamp":1726185600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["PTDC\/BII-BIO\/31761\/2017"],"award-info":[{"award-number":["PTDC\/BII-BIO\/31761\/2017"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["10.54499\/UIDP\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDP\/04378\/2020"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["10.54499\/UIDB\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDB\/04378\/2020"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"Foundation for Science and Technology (FCT, Portugal)","award":["SFRH\/BPD\/124612\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/124612\/2016"]}]},{"name":"Research Unit on Applied Molecular Biosciences-UCIBIO","award":["PTDC\/BII-BIO\/31761\/2017"],"award-info":[{"award-number":["PTDC\/BII-BIO\/31761\/2017"]}]},{"name":"Research Unit on Applied Molecular Biosciences-UCIBIO","award":["10.54499\/UIDP\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDP\/04378\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences-UCIBIO","award":["10.54499\/UIDB\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDB\/04378\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences-UCIBIO","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"Research Unit on Applied Molecular Biosciences-UCIBIO","award":["SFRH\/BPD\/124612\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/124612\/2016"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy-i4HB","award":["PTDC\/BII-BIO\/31761\/2017"],"award-info":[{"award-number":["PTDC\/BII-BIO\/31761\/2017"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy-i4HB","award":["10.54499\/UIDP\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDP\/04378\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy-i4HB","award":["10.54499\/UIDB\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDB\/04378\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy-i4HB","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"Associate Laboratory Institute for Health and Bioeconomy-i4HB","award":["SFRH\/BPD\/124612\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/124612\/2016"]}]},{"name":"FCT\/MCTES","award":["PTDC\/BII-BIO\/31761\/2017"],"award-info":[{"award-number":["PTDC\/BII-BIO\/31761\/2017"]}]},{"name":"FCT\/MCTES","award":["10.54499\/UIDP\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDP\/04378\/2020"]}]},{"name":"FCT\/MCTES","award":["10.54499\/UIDB\/04378\/2020"],"award-info":[{"award-number":["10.54499\/UIDB\/04378\/2020"]}]},{"name":"FCT\/MCTES","award":["LA\/P\/0140\/2020"],"award-info":[{"award-number":["LA\/P\/0140\/2020"]}]},{"name":"FCT\/MCTES","award":["SFRH\/BPD\/124612\/2016"],"award-info":[{"award-number":["SFRH\/BPD\/124612\/2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Biotechnological active peptides are gaining interest in the cosmetics industry due to their antimicrobial, anti-inflammatory, antioxidant, and anti-collagenase (ACE) effects, as well as wound healing properties, making them suitable for cosmetic formulations. The antimicrobial activity of peptides (2\u201310 kDa) secreted by Saccharomyces cerevisiae Ethanol-Red was evaluated against dermal pathogens using broth microdilution and challenge tests. ACE was assessed using a collagenase activity colorimetric assay, antioxidant activity via spectrophotometric monitoring of nitrotetrazolium blue chloride (NBT) reduction, and anti-inflammatory effects by quantifying TNF-\u03b1 mRNA in lipopolysaccharides (LPS)-exposed dermal fibroblasts. Wound healing assays involved human fibroblasts, endothelial cells, and dermal keratinocytes. The peptides (2\u201310 kDa) exhibited antimicrobial activity against 10 dermal pathogens, with the Minimum Inhibitory Concentrations (MICs) ranging from 125 \u00b5g\/mL for Staphylococcus aureus to 1000 \u00b5g\/mL for Candida albicans and Streptococcus pyogenes. In the challenge test, peptides at their MICs reduced microbial counts significantly, fulfilling ISO 11930:2019 standards, except against Aspergillus brasiliensis. The peptides combined with Microcare\u24c7 SB showed synergy, particularly against C. albicans and A. brasilensis. In vitro, the peptides inhibited collagenase activity by 41.8% and 94.5% at 250 and 1000 \u00b5g\/mL, respectively, and demonstrated antioxidant capacity. Pre-incubation with peptides decreased TNF-\u03b1 expression in fibroblasts, indicating anti-inflammatory effects. The peptides do not show to promote or inhibit the angiogenesis of endothelial cells, but are able to attenuate fibrosis, scar formation, and chronic inflammation during the final phases of the wound healing process. The peptides showed antimicrobial, antioxidant, ACE, and anti-inflammatory properties, highlighting their potential as multifunctional bioactive ingredients in skincare, warranting further optimization and exploration in cosmetic applications.<\/jats:p>","DOI":"10.3390\/antibiotics13090881","type":"journal-article","created":{"date-parts":[[2024,9,13]],"date-time":"2024-09-13T06:39:16Z","timestamp":1726209556000},"page":"881","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Evaluation of Biotechnological Active Peptides Secreted by Saccharomyces cerevisiae with Potential Skin Benefits"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3959-2993","authenticated-orcid":false,"given":"Elisabete Muchagato","family":"Maur\u00edcio","sequence":"first","affiliation":[{"name":"BIORG\u2014Bioengineering and Sustainability Research Group, Faculdade de Engenharia, Universidade Lus\u00f3fona, Av. Campo Grande 376, 1749-024 Lisbon, Portugal"},{"name":"CBIOS\u2014Research Center for Biosciences & Health Technologies, Universidade Lus\u00f3fona, Campo Grande 376, 1749-024 Lisbon, Portugal"},{"name":"Elisa C\u00e2mara, Lda, Dermocosm\u00e9tica, Centro Empresarial de Tala\u00edde, n\u00b07 e 8, 2785-723 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1760-6899","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Branco","sequence":"additional","affiliation":[{"name":"BIORG\u2014Bioengineering and Sustainability Research Group, Faculdade de Engenharia, Universidade Lus\u00f3fona, Av. Campo Grande 376, 1749-024 Lisbon, Portugal"},{"name":"Linking Landscape, Environment, Agriculture and Food (LEAF), Associated Laboratory TERRA, Instituto Superior de Agronomia, University of Lisbon, Tapada da Ajuda, 1349-017 Lisbon, Portugal"},{"name":"Unit of Bioenergy and Biorefinary, Laborat\u00f3rio Nacional de Energia e Geologia (LNEG), Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Luiza Barros","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"BIORG\u2014Bioengineering and Sustainability Research Group, Faculdade de Engenharia, Universidade Lus\u00f3fona, Av. Campo Grande 376, 1749-024 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8676-6562","authenticated-orcid":false,"given":"Catarina","family":"Roma-Rodrigues","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department Ci\u00eancias da Vida, NOVA School of Science and Technology, 2829-516 Caparica, Portugal"},{"name":"i4HB, Associate Laboratory\u2014Institute for Health and Bioeconomy, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1424-7229","authenticated-orcid":false,"given":"Katelene","family":"Lima","sequence":"additional","affiliation":[{"name":"Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Paula","family":"Duarte","sequence":"additional","affiliation":[{"name":"The Mechanical Engineering and Resource Sustainability Center (MEtRICs), Chemistry Department, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2054-4438","authenticated-orcid":false,"given":"Alexandra R.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"UCIBIO\u2014Applied Molecular Biosciences Unit, Department Ci\u00eancias da Vida, NOVA School of Science and Technology, 2829-516 Caparica, Portugal"},{"name":"i4HB, Associate Laboratory\u2014Institute for Health and Bioeconomy, Faculdade de Ci\u00eancias e Tecnologia, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9062-9827","authenticated-orcid":false,"given":"Helena","family":"Albergaria","sequence":"additional","affiliation":[{"name":"Unit of Bioenergy and Biorefinary, Laborat\u00f3rio Nacional de Energia e Geologia (LNEG), Estrada do Pa\u00e7o do Lumiar, 22, 1649-038 Lisbon, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Alves, A., Sousa, E., Kijjoa, A., and Pinto, M. 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