{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T14:29:34Z","timestamp":1780324174793,"version":"3.54.1"},"reference-count":95,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,17]],"date-time":"2024-09-17T00:00:00Z","timestamp":1726531200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Coordination for the Improvement of Higher Education Personnel\u2014CAPES","award":["88887.803735\/2023-00"],"award-info":[{"award-number":["88887.803735\/2023-00"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel\u2014CAPES","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordination for the Improvement of Higher Education Personnel\u2014CAPES","award":["302271\/2023-6"],"award-info":[{"award-number":["302271\/2023-6"]}]},{"name":"CAPES","award":["88887.803735\/2023-00"],"award-info":[{"award-number":["88887.803735\/2023-00"]}]},{"name":"CAPES","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CAPES","award":["302271\/2023-6"],"award-info":[{"award-number":["302271\/2023-6"]}]},{"DOI":"10.13039\/501100003593","name":"The National Council for Scientific and Technological Development\u2014CNPq","doi-asserted-by":"publisher","award":["88887.803735\/2023-00"],"award-info":[{"award-number":["88887.803735\/2023-00"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"The National Council for Scientific and Technological Development\u2014CNPq","doi-asserted-by":"publisher","award":["001"],"award-info":[{"award-number":["001"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"The National Council for Scientific and Technological Development\u2014CNPq","doi-asserted-by":"publisher","award":["302271\/2023-6"],"award-info":[{"award-number":["302271\/2023-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Onychomycosis is a common fungal nail infection for which new antifungals are needed to overcome antimicrobial resistance and the limitations of conventional treatments. This study reports the development of antifungal nail lacquers containing oregano essential oil (OEO), rosemary essential oil (REO), and biogenic silver nanoparticles (bioAgNPs). The formulations (F) were tested against dermatophytes using agar diffusion, ex vivo nail infection, and scanning electron microscopy techniques. They were evaluated for their pharmacotechnical characteristics and by FTIR-PAS to assess permeation across the nail. F-OEO and F-OEO\/bioAgNPs were promising candidates for the final nail lacquer formulation, as they permeated through the nail and showed antifungal efficacy against dermatophytes-contaminated nails after 5 days of treatment. Treated nails exhibited decreased hyphae and spores compared to the untreated control; the hyphae were atypically flattened, indicating loss of cytoplasmic content due to damage to the cytoplasmic membrane. The formulations were stable after centrifugation and thermal stress, maintaining organoleptic and physicochemical characteristics. Both F-OEO and F-OEO\/bioAgNPs had pH compatible with the nail and drying times (59\u201390 s) within the reference for nail lacquer. For the first time, OEO and bioAgNPs were incorporated into nail lacquer, resulting in a natural and nanotechnological product for onychomycosis that could combat microbial resistance.<\/jats:p>","DOI":"10.3390\/antibiotics13090892","type":"journal-article","created":{"date-parts":[[2024,9,18]],"date-time":"2024-09-18T01:52:31Z","timestamp":1726624351000},"page":"892","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Nail Lacquer Containing Origanum vulgare and Rosmarinus officinalis Essential Oils and Biogenic Silver Nanoparticles for Onychomycosis: Development, Characterization, and Evaluation of Antifungal Efficacy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4012-9100","authenticated-orcid":false,"given":"Sara","family":"Scandorieiro","sequence":"first","affiliation":[{"name":"Laboratory of Innovation and Cosmeceutical Technology, Department of Pharmaceutical Sciences, Center of Health Sciences, Hospital Universit\u00e1rio de Londrina, Robert Koch Avenue, 60, Londrina 86038-350, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia Rodrigues","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Innovation and Cosmeceutical Technology, Department of Pharmaceutical Sciences, Center of Health Sciences, Hospital Universit\u00e1rio de Londrina, Robert Koch Avenue, 60, Londrina 86038-350, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monique","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Physics, Center of Exact Sciences, Universidade Estadual de Maring\u00e1, Colombo Avenue, 5790, Maring\u00e1 87020-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5118-5370","authenticated-orcid":false,"given":"Lidiane Vizioli","family":"de Castro-Hoshino","sequence":"additional","affiliation":[{"name":"Department of Physics, Center of Exact Sciences, Universidade Estadual de Maring\u00e1, Colombo Avenue, 5790, Maring\u00e1 87020-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6017-2582","authenticated-orcid":false,"given":"Mauro Luciano","family":"Baesso","sequence":"additional","affiliation":[{"name":"Department of Physics, Center of Exact Sciences, Universidade Estadual de Maring\u00e1, Colombo Avenue, 5790, Maring\u00e1 87020-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8624-795X","authenticated-orcid":false,"given":"Gerson","family":"Nakazato","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR-445, Km 380, University Campus, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7170-2155","authenticated-orcid":false,"given":"Renata Katsuko Takayama","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR-445, Km 380, University Campus, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano Aparecido","family":"Panagio","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Mycology and Oral Microbiology, Department of Microbiology, Center of Biological Sciences, Universidade Estadual de Londrina, Celso Garcia Cid Road, PR-445, Km 380, University Campus, Londrina 86057-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6498-2806","authenticated-orcid":false,"given":"Audrey Alesandra Stinghen Garcia","family":"Lonni","sequence":"additional","affiliation":[{"name":"Laboratory of Innovation and Cosmeceutical Technology, Department of Pharmaceutical Sciences, Center of Health Sciences, Hospital Universit\u00e1rio de Londrina, Robert Koch Avenue, 60, Londrina 86038-350, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1111\/myc.12592","article-title":"Understanding the Formidable Nail Barrier: A Review of the Nail Microstructure, Composition and Diseases","volume":"60","author":"Baswan","year":"2017","journal-title":"Mycoses"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1080\/07853890.2022.2044511","article-title":"Optimal Diagnosis and Management of Common Nail Disorders","volume":"54","author":"Lee","year":"2022","journal-title":"Ann. 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