{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T00:52:29Z","timestamp":1793839949898,"version":"4.6.1"},"reference-count":76,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T00:00:00Z","timestamp":1727395200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Recovery and Resilience Plan","award":["ECS00000017"],"award-info":[{"award-number":["ECS00000017"]}]},{"name":"National Recovery and Resilience Plan","award":["CUP I53C22000780001"],"award-info":[{"award-number":["CUP I53C22000780001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Mycobacterial infections caused by tuberculous and non-tuberculous strains pose significant treatment challenges, especially among immunocompromised patients. Conventional antibiotic therapies often fail due to bacterial resistance, highlighting the need for alternative therapeutic strategies. Mycobacteriophages are emerging as promising candidates for the treatment of mycobacteria. This review comprehensively explores phage isolation, characterization, and clinical applications. Despite the need for more extensive in vitro and in vivo studies, existing evidence shows their efficacy against both sensitive and antibiotic-resistant mycobacterial strains, even under disease-mimicking conditions, particularly when used in cocktails to minimize resistance development. Mycobacteriophages can be engineered and evolved to overcome limitations associated with lysogeny and narrow host range. Furthermore, they exhibit activity in ex vivo and in vivo infection models, successfully targeting mycobacteria residing within macrophages. Delivery methods such as bacterial and liposomal vectors facilitate their entry into human cells. Considering the potential for phage-treatment-induced bacterial resistance, as described in this review, the combination of mycobacteriophages with antibiotics shows efficacy in countering mycobacterial growth, both in the laboratory setting and in animal models. Interestingly, phage-encoded products can potentiate the activity of relevant antibiotics. Finally, the application of phages in different compassionate cases is reported. The positive outcomes indicate that phage therapy represents a promising solution for the treatment of antibiotic-resistant mycobacteria.<\/jats:p>","DOI":"10.3390\/antibiotics13100926","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T09:16:10Z","timestamp":1727428570000},"page":"926","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mycobacteriophages and Their Applications"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5109-388X","authenticated-orcid":false,"given":"Andrea","family":"Bonacorsi","sequence":"first","affiliation":[{"name":"Department of Biology, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Ferretti","sequence":"additional","affiliation":[{"name":"Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0688-034X","authenticated-orcid":false,"given":"Mariagrazia","family":"Di Luca","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7036-1192","authenticated-orcid":false,"given":"Laura","family":"Rindi","sequence":"additional","affiliation":[{"name":"Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Kanabalan, R.D., Lee, L.J., Lee, T.Y., Chong, P.P., Hassan, L., Ismail, R., and Chin, V.K. (2021). Human Tuberculosis and Mycobacterium Tuberculosis Complex: A Review on Genetic Diversity, Pathogenesis and Omics Approaches in Host Biomarkers Discovery. Microbiol. Res., 246.","DOI":"10.1016\/j.micres.2020.126674"},{"key":"ref_2","unstructured":"WHO (2023). Global Tuberculosis Report 2023, World Health Organization."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1370","DOI":"10.5588\/ijtld.14.0120","article-title":"The Epidemiologic Relationship between Tuberculosis and Non-Tuberculous Mycobacterial Disease: A Systematic Review","volume":"18","author":"Brode","year":"2014","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.ccm.2023.07.001","article-title":"Environmental Sources and Transmission of Nontuberculous Mycobacteria","volume":"44","author":"Honda","year":"2023","journal-title":"Clin. Chest Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1164\/ajrccm.185.2.231","article-title":"Respiratory Outbreak of Mycobacterium abscessus Subspecies Massiliense in a Lung Transplant and Cystic Fibrosis Center","volume":"185","author":"Aitken","year":"2012","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1080\/17476348.2023.2283135","article-title":"A Narrative Review of Nontuberculous Mycobacterial Pulmonary Disease: Microbiology, Epidemiology, Diagnosis, and Management Challenges","volume":"17","author":"Hamed","year":"2023","journal-title":"Expert Rev. Respir. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Alsayed, S.S.R., and Gunosewoyo, H. (2023). Tuberculosis: Pathogenesis, Current Treatment Regimens and New Drug Targets. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24065202"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2000535","DOI":"10.1183\/13993003.00535-2020","article-title":"Treatment of Nontuberculous Mycobacterial Pulmonary Disease: An Official ATS\/ERS\/ESCMID\/IDSA Clinical Practice Guideline","volume":"56","author":"Daley","year":"2020","journal-title":"Eur. Respir. J."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Khawbung, J.L., Nath, D., and Chakraborty, S. (2021). Drug Resistant Tuberculosis: A Review. Comp. Immunol. Microbiol. Infect. Dis., 74.","DOI":"10.1016\/j.cimid.2020.101574"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Saxena, S., Spaink, H.P., and Forn-Cun\u00ed, G. (2021). Drug Resistance in Nontuberculous Mycobacteria: Mechanisms and Models. Biology, 10.","DOI":"10.3390\/biology10020096"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S423","DOI":"10.1093\/cid\/ciad515","article-title":"Use of Bacteriophages to Target Intracellular Pathogens","volume":"77","author":"Venturini","year":"2023","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2150449","DOI":"10.1080\/21505594.2022.2150449","article-title":"Pathogenicity and Virulence of Mycobacterium tuberculosis","volume":"14","author":"Rahlwes","year":"2023","journal-title":"Virulence"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"104665","DOI":"10.1016\/j.meegid.2020.104665","article-title":"Genomic and Proteomic Portrait of a Novel Mycobacteriophage SWU2 Isolated from China","volume":"87","author":"Gong","year":"2021","journal-title":"Infect. Genet. Evol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"105417","DOI":"10.1016\/j.meegid.2023.105417","article-title":"Characterization and Genome Analysis of G1 Sub-Cluster Mycobacteriophage Lang","volume":"109","author":"Lang","year":"2023","journal-title":"Infect. Genet. Evol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Nayak, T., Kakkar, A., Singh, R.K., Jaiswal, L.K., Singh, A.K., Temple, L., and Gupta, A. (2023). Isolation and Characterization of a Novel Mycobacteriophage Kashi-VT1 Infecting Mycobacterium Species. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1173894"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"170","DOI":"10.4103\/ijmy.ijmy_42_19","article-title":"Study of Characteristics of Mycobacteriophage\u2014A Novel Tool to Treat Mycobacterium spp","volume":"8","author":"Satish","year":"2019","journal-title":"Int. J. Mycobacteriol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"197884","DOI":"10.1016\/j.virusres.2020.197884","article-title":"Characterization and Genome Analysis of B1 Sub-Cluster Mycobacteriophage PDRPxv","volume":"279","author":"Sinha","year":"2020","journal-title":"Virus Res."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Cao Yao, J.C., Garcia Cehic, D., Quer, J., M\u00e9ndez, J.N., Gorr\u00edn, A.D., Hevia, L.G., and Fern\u00e1ndez, M.T.T. (2024). Complete Genome Sequences of Four Mycobacteriophages Involved in Directed Evolution against Undisputed Mycobacterium abscessus Clinical Strains. Microorganisms, 12.","DOI":"10.3390\/microorganisms12020374"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e00337-22","DOI":"10.1128\/jb.00337-22","article-title":"Mycobacterium Smegmatis: The Vanguard of Mycobacterial Research","volume":"205","author":"Sparks","year":"2023","journal-title":"J. Bacteriol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"784","DOI":"10.1093\/bioinformatics\/btw711","article-title":"PhagesDB: The Actinobacteriophage Database","volume":"33","author":"Russell","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s41030-022-00210-y","article-title":"Phage Therapy for Nontuberculous Mycobacteria: Challenges and Opportunities","volume":"9","author":"Hatfull","year":"2023","journal-title":"Pulm. Ther."},{"key":"ref_22","first-page":"e000079","article-title":"Evolutionary Interpretations of Mycobacteriophage Biodiversity and Host-Range through the Analysis of Codon Usage Bias","volume":"2","author":"Esposito","year":"2016","journal-title":"Microb. Genom."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"10-1128","DOI":"10.1128\/microbiolspec.GPP3-0026-2018","article-title":"Mycobacteriophages","volume":"6","author":"Hatfull","year":"2018","journal-title":"Microbiol. Spectr."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.cell.2022.11.017","article-title":"Phage Therapy: From Biological Mechanisms to Future Directions","volume":"186","author":"Strathdee","year":"2023","journal-title":"Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1146\/annurev-virology-122019-070009","article-title":"Actinobacteriophages: Genomics, Dynamics, and Applications","volume":"7","author":"Hatfull","year":"2020","journal-title":"Annu. Rev. Virol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.virol.2012.09.026","article-title":"On the Nature of Mycobacteriophage Diversity and Host Preference","volume":"434","author":"Marinelli","year":"2012","journal-title":"Virology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e00973-21","DOI":"10.1128\/mBio.00973-21","article-title":"Toward a Phage Cocktail for Tuberculosis: Susceptibility and Tuberculocidal Action of Mycobacteriophages against Diverse Mycobacterium Tuberculosis Strains","volume":"12","author":"Dedrick","year":"2021","journal-title":"mBio"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e01421-20","DOI":"10.1128\/MRA.01421-20","article-title":"Genome Sequence of Mycobacterium Abscessus Phage phiT46-1","volume":"10","author":"Amarh","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e00155-21","DOI":"10.1128\/MRA.00155-21","article-title":"Genome Sequence of Mycobacterium Abscessus Phage phiT45-1","volume":"10","author":"Amarh","year":"2021","journal-title":"Microbiol. Resour. Announc."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1099\/jmm.0.46238-0","article-title":"Host Range of 14 Mycobacteriophages in Mycobacterium Ulcerans and Seven Other Mycobacteria Including Mycobacterium Tuberculosis\u2014Application for Identification and Susceptibility Testing","volume":"55","author":"Rybniker","year":"2006","journal-title":"J. Med. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1007\/s00203-014-0954-6","article-title":"Characterization and Classification of Bo4 as a Cluster G Mycobacteriophage That Can Infect and Lyse, M. Tuberculosis","volume":"196","author":"Gan","year":"2014","journal-title":"Arch. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.gene.2015.02.053","article-title":"Genomic and Proteomic Features of Mycobacteriophage SWU1 Isolated from China Soil","volume":"561","author":"Fan","year":"2015","journal-title":"Gene"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Kalapala, Y.C., Sharma, P.R., and Agarwal, R. (2020). Antimycobacterial Potential of Mycobacteriophage Under Disease-Mimicking Conditions. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.583661"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"4714","DOI":"10.1128\/JB.00305-09","article-title":"Acid Resistance in Mycobacterium tuberculosis","volume":"191","author":"Vandal","year":"2009","journal-title":"J. Bacteriol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.coviro.2021.12.009","article-title":"Endocytosis of Bacteriophages","volume":"52","author":"Miernikiewicz","year":"2022","journal-title":"Curr. Opin. Virol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1155","DOI":"10.1086\/343812","article-title":"Killing of Mycobacterium avium and Mycobacterium tuberculosis by a Mycobacteriophage Delivered by a Nonvirulent Mycobacterium: A Model for Phage Therapy of Intracellular Bacterial Pathogens","volume":"186","author":"Broxmeyer","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1089\/mdr.2006.12.1","article-title":"In Vivo Efficacy of Phage Therapy for Mycobacterium Avium Infection as Delivered by a Nonvirulent Mycobacterium","volume":"12","author":"Danelishvili","year":"2006","journal-title":"Microb. Drug Resist."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1517\/17425247.2015.1043125","article-title":"A First Step toward Liposome-Mediated Intracellular Bacteriophage Therapy","volume":"12","author":"Nieth","year":"2015","journal-title":"Expert Opin. Drug Deliv."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1007\/s10517-020-04887-6","article-title":"Bactericidal Activity of Liposomal Form of Lytic Mycobacteriophage D29 in Cell Models of Tuberculosis Infection In Vitro","volume":"169","author":"Lapenkova","year":"2020","journal-title":"Bull. Exp. Biol. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"e02924-23","DOI":"10.1128\/mbio.02924-23","article-title":"Bacteriophage Infection and Killing of Intracellular Mycobacterium abscessus","volume":"15","author":"Schmalstig","year":"2024","journal-title":"mBio"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1146\/annurev-virology-012423-110530","article-title":"Developing Phage Therapy That Overcomes the Evolution of Bacterial Resistance","volume":"10","author":"Antani","year":"2023","journal-title":"Annu. Rev. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Guti\u00e9rrez, A.V., Viljoen, A., Ghigo, E., Herrmann, J.-L., and Kremer, L. (2018). Glycopeptidolipids, a Double-Edged Sword of the Mycobacterium abscessus Complex. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01145"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1717","DOI":"10.1038\/s41564-023-01451-6","article-title":"Therapeutically Useful Mycobacteriophages BPs and Muddy Require Trehalose Polyphleates","volume":"8","author":"Wetzel","year":"2023","journal-title":"Nat. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"e03431-20","DOI":"10.1128\/mBio.03431-20","article-title":"Mycobacterium abscessus Strain Morphotype Determines Phage Susceptibility, the Repertoire of Therapeutically Useful Phages, and Phage Resistance","volume":"12","author":"Dedrick","year":"2021","journal-title":"mBio"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1335.e9","DOI":"10.1016\/j.cmi.2023.06.026","article-title":"The Problem of Mycobacterium abscessus Complex: Multi-Drug Resistance, Bacteriophage Susceptibility and Potential Healthcare Transmission","volume":"29","author":"Dedrick","year":"2023","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"6938","DOI":"10.1093\/nar\/gkac505","article-title":"Decoding Phage Resistance by Mpr and Its Role in Survivability of Mycobacterium smegmatis","volume":"50","author":"Seniya","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1046\/j.1365-2958.1996.6291342.x","article-title":"Characterization of a Mycobacterium smegmatis Gene That Confers Resistance to Phages L5 and D29 When Overexpressed","volume":"21","author":"Barsom","year":"1996","journal-title":"Mol. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"dmm049159","DOI":"10.1242\/dmm.049159","article-title":"Mycobacteriophage\u2013Antibiotic Therapy Promotes Enhanced Clearance of Drug-Resistant Mycobacterium abscessus","volume":"14","author":"Johansen","year":"2021","journal-title":"Dis. Models Mech."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Jiang, Z., Wei, J., Liang, Y., Peng, N., and Li, Y. (2020). Aminoglycoside Antibiotics Inhibit Mycobacteriophage Infection. Antibiotics, 9.","DOI":"10.1101\/2020.07.03.185546"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Catal\u00e3o, M.J., and Pimentel, M. (2018). Mycobacteriophage Lysis Enzymes: Targeting the Mycobacterial Cell Envelope. Viruses, 10.","DOI":"10.3390\/v10080428"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e04597-22","DOI":"10.1128\/spectrum.04597-22","article-title":"Mycobacteriophage D29 Lysin B Exhibits Promising Anti-Mycobacterial Activity against Drug-Resistant Mycobacterium tuberculosis","volume":"11","author":"Singh","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00253-022-12306-1","article-title":"Production, Characterization, and Application of Phage-Derived PK34 Recombinant Anti-Microbial Peptide","volume":"107","author":"Wang","year":"2023","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Li, Q., Zhou, M., Fan, X., Yan, J., Li, W., and Xie, J. (2016). Mycobacteriophage SWU1 Gp39 Can Potentiate Multiple Antibiotics against Mycobacterium via Altering the Cell Wall Permeability. Sci. Rep., 6.","DOI":"10.1038\/srep28701"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"8169","DOI":"10.1007\/s00253-016-7681-7","article-title":"Mycobacteriophage Putative GTPase-Activating Protein Can Potentiate Antibiotics","volume":"100","author":"Yan","year":"2016","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.tube.2019.01.002","article-title":"Mycobacteriophage ZoeJ: A Broad Host-Range Close Relative of Mycobacteriophage TM4","volume":"115","author":"Dedrick","year":"2019","journal-title":"Tuberculosis"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Marinelli, L.J., Piuri, M., Swigo\u0148ov\u00e1, Z., Balachandran, A., Oldfield, L.M., Van Kessel, J.C., and Hatfull, G.F. (2008). BRED: A Simple and Powerful Tool for Constructing Mutant and Recombinant Bacteriophage Genomes. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0003957"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1038\/nmeth996","article-title":"Recombineering in Mycobacterium Tuberculosis","volume":"4","author":"Hatfull","year":"2007","journal-title":"Nat. Methods"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hashemi Shahraki, A., and Mirsaeidi, M. (2021). Phage Therapy for Mycobacterium abscessus and Strategies to Improve Outcomes. Microorganisms, 9.","DOI":"10.3390\/microorganisms9030596"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Cebri\u00e1-Mendoza, M., Sanju\u00e1n, R., and Domingo-Calap, P. (2019). Directed Evolution of a Mycobacteriophage. Antibiotics, 8.","DOI":"10.3390\/antibiotics8020046"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Burrowes, B., Molineux, I., and Fralick, J. (2019). Directed in Vitro Evolution of Therapeutic Bacteriophages: The Appelmans Protocol. Viruses, 11.","DOI":"10.3390\/v11030241"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"ofad051","DOI":"10.1093\/ofid\/ofad051","article-title":"Application of Phage Therapy in a Case of a Chronic Hip-Prosthetic Joint Infection Due to Pseudomonas aeruginosa: An Italian Real-Life Experience and In Vitro Analysis","volume":"10","author":"Cesta","year":"2023","journal-title":"Open Forum Infect. Dis."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1998","DOI":"10.1093\/cid\/ciz782","article-title":"Eradication of a Multidrug-Resistant, Carbapenemase-Producing Klebsiella Pneumoniae Isolate Following Oral and Intra-Rectal Therapy with a Custom Made, Lytic Bacteriophage Preparation","volume":"70","author":"Corbellino","year":"2020","journal-title":"Clin. Infect. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Doub, J.B., Ng, V.Y., Lee, M., Chi, A., Lee, A., W\u00fcrstle, S., and Chan, B. (2022). Salphage: Salvage Bacteriophage Therapy for Recalcitrant MRSA Prosthetic Joint Infection. Antibiotics, 11.","DOI":"10.3390\/antibiotics11050616"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1860","DOI":"10.1016\/j.cell.2022.04.024","article-title":"Host and Pathogen Response to Bacteriophage Engineered against Mycobacterium abscessus Lung Infection","volume":"185","author":"Nick","year":"2022","journal-title":"Cell"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1093\/cid\/ciac453","article-title":"Phage Therapy of Mycobacterium Infections: Compassionate Use of Phages in 20 Patients with Drug-Resistant Mycobacterial Disease","volume":"76","author":"Dedrick","year":"2023","journal-title":"Clin. Infect. Dis."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1038\/s41591-019-0437-z","article-title":"Engineered Bacteriophages for Treatment of a Patient with a Disseminated Drug-Resistant Mycobacterium abscessus","volume":"25","author":"Dedrick","year":"2019","journal-title":"Nat. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1038\/s41591-021-01403-9","article-title":"Potent Antibody-Mediated Neutralization Limits Bacteriophage Treatment of a Pulmonary Mycobacterium abscessus Infection","volume":"27","author":"Dedrick","year":"2021","journal-title":"Nat. Med."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"ofac194","DOI":"10.1093\/ofid\/ofac194","article-title":"Nebulized Bacteriophage in a Patient with Refractory Mycobacterium abscessus Lung Disease","volume":"9","author":"Dedrick","year":"2022","journal-title":"Open Forum Infect. Dis."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"2313","DOI":"10.1038\/s41467-022-29689-4","article-title":"Bacteriophage Treatment of Disseminated Cutaneous Mycobacterium chelonae Infection","volume":"13","author":"Little","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1146\/annurev-med-080219-122208","article-title":"Phage Therapy for Antibiotic-Resistant Bacterial Infections","volume":"73","author":"Hatfull","year":"2022","journal-title":"Annu. Rev. Med."},{"key":"ref_71","doi-asserted-by":"crossref","unstructured":"Shield, C.G., Swift, B.M.C., McHugh, T.D., Dedrick, R.M., Hatfull, G.F., and Satta, G. (2021). Application of Bacteriophages for Mycobacterial Infections, from Diagnosis to Treatment. Microorganisms, 9.","DOI":"10.3390\/microorganisms9112366"},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Singla, S., Harjai, K., Katare, O.P., and Chhibber, S. (2016). Encapsulation of Bacteriophage in Liposome Accentuates Its Entry in to Macrophage and Shields It from Neutralizing Antibodies. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0153777"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"2084","DOI":"10.1007\/s11095-017-2213-4","article-title":"Anti-Tuberculosis Bacteriophage D29 Delivery with a Vibrating Mesh Nebulizer, Jet Nebulizer, and Soft Mist Inhaler","volume":"34","author":"Carrigy","year":"2017","journal-title":"Pharm. Res."},{"key":"ref_74","doi-asserted-by":"crossref","unstructured":"Reece, E., Bettio, P.H.D.A., and Renwick, J. (2021). Polymicrobial Interactions in the Cystic Fibrosis Airway Microbiome Impact the Antimicrobial Susceptibility of Pseudomonas Aeruginosa. Antibiotics, 10.","DOI":"10.3390\/antibiotics10070827"},{"key":"ref_75","doi-asserted-by":"crossref","unstructured":"Campo-P\u00e9rez, V., Juli\u00e1n, E., and Torrents, E. (2024). Interplay of Mycobacterium abscessus and Pseudomonas aeruginosa in Coinfection: Biofilm Dynamics and Host Immune Response. bioRxiv.","DOI":"10.1101\/2024.01.22.576702"},{"key":"ref_76","doi-asserted-by":"crossref","unstructured":"Recchia, D., Stelitano, G., Stamilla, A., Gutierrez, D.L., Degiacomi, G., Chiarelli, L.R., and Pasca, M.R. (2023). Mycobacterium Abscessus Infections in Cystic Fibrosis Individuals: A Review on Therapeutic Options. Int. J. Mol. Sci., 24.","DOI":"10.3390\/ijms24054635"}],"container-title":["Antibiotics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2079-6382\/13\/10\/926\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:05:12Z","timestamp":1760112312000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2079-6382\/13\/10\/926"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,27]]},"references-count":76,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2024,10]]}},"alternative-id":["antibiotics13100926"],"URL":"https:\/\/doi.org\/10.3390\/antibiotics13100926","relation":{},"ISSN":["2079-6382"],"issn-type":[{"value":"2079-6382","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,27]]}}}