{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:23:41Z","timestamp":1793021021507,"version":"4.5.0"},"reference-count":95,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T00:00:00Z","timestamp":1749168000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["RED-00132-16"],"award-info":[{"award-number":["RED-00132-16"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico (CNPq)","award":["RED 00131-23"],"award-info":[{"award-number":["RED 00131-23"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa de Minas Gerais","award":["RED-00132-16"],"award-info":[{"award-number":["RED-00132-16"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa de Minas Gerais","award":["RED 00131-23"],"award-info":[{"award-number":["RED 00131-23"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["RED-00132-16"],"award-info":[{"award-number":["RED-00132-16"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["RED 00131-23"],"award-info":[{"award-number":["RED 00131-23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Antimicrobial resistance is a major global health threat. Among the most problematic pathogens are carbapenem-resistant Acinetobacter baumannii and Klebsiella pneumoniae, which are significant causes of mortality in humans, particularly in the context of nosocomial infections. In companion animals, these bacteria have been reported mainly as colonizers of healthy animals or, less frequently, in community-acquired infections. However, no confirmed cases of healthcare-associated infections caused by these species have been documented in this population. This study reports the first confirmed fatal cases of infection with carbapenem-resistant A. baumannii and KPC-producing K. pneumoniae in dogs. Methods: Three hospitalized dogs developed infections associated with distinct anatomical devices, including a venous catheter, an endotracheal tube, and a Penrose drain. Bacterial isolation followed by antimicrobial susceptibility testing identified carbapenem-resistant A. baumannii and K. pneumoniae. The isolates were subsequently subjected to additional antimicrobial resistance tests and whole-genome sequencing (WGS). Results: WGS confirmed the presence of the OXA-23 carbapenemase gene in both A. baumannii isolates and the KPC-2 carbapenemase gene was detected in the K. pneumoniae strain. All three strains exhibited resistance to multiple antimicrobial classes, including \u03b2-lactams (amoxicillin-clavulanic acid, ampicillin, cephalotin, piperacillin-tazobactam, cefoxitin, ceftiofur, cefotaxime, ertapenem, imipenem and meropenem), aminoglycosides (gentamicin, neomycin), tetracyclines (doxycycline, tetracycline and oxytetracycline), fluoroquinolones (ciprofloxacin, enrofloxacin), and folate pathway antagonists (trimethoprim-sulfamethoxazole). Multilocus sequence typing identified two high-risk clones: K. pneumoniae ST340 (CC258) and A. baumannii ST15 (CC15). Single nucleotide polymorphism analysis confirmed a high degree of genetic similarity between these isolates and strains previously associated with human infections in Brazil. Conclusions: These findings provide the first evidence of fatal, healthcare-associated infections caused by these multidrug-resistant pathogens in dogs and underscore the need to strengthen surveillance and infection control practices in veterinary hospitals. Furthermore, the results raise concerns about the potential of companion animals to act as reservoirs for multidrug-resistant organisms of public health relevance.<\/jats:p>","DOI":"10.3390\/antibiotics14060584","type":"journal-article","created":{"date-parts":[[2025,6,6]],"date-time":"2025-06-06T09:02:03Z","timestamp":1749200523000},"page":"584","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Genomic Characterization of Carbapenem-Resistant Acinetobacter baumannii (OXA-23) and Klebsiella pneumoniae (KPC-2) Causing Hospital-Acquired Infections in Dogs"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6098-0907","authenticated-orcid":false,"given":"Isabela P\u00e1dua","family":"Zanon","sequence":"first","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0902-0000","authenticated-orcid":false,"given":"Jo\u00e3o Victor Ferreira","family":"Campos","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4672-9812","authenticated-orcid":false,"given":"Yasmin Gon\u00e7alves de","family":"Castro","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora Maria Soares de","family":"Melo","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1067-1882","authenticated-orcid":false,"given":"Fl\u00e1via Figueira","family":"Aburjaile","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7635-9656","authenticated-orcid":false,"given":"Bertram","family":"Brenig","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine, University of G\u00f6ttingen, 37077 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4775-2280","authenticated-orcid":false,"given":"Vasco","family":"Azevedo","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Biol\u00f3gicas (ICB), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0074-2488","authenticated-orcid":false,"given":"Rodrigo Ot\u00e1vio Silveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Medicina Veterin\u00e1ria Preventiva, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte 30720440, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Jiang, Y., Ding, Y., Wei, Y., Jian, C., Liu, J., and Zeng, Z. 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