{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:02:44Z","timestamp":1788346964056,"version":"build-2803163510"},"reference-count":71,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy in Cluj-Napoca, Romania","award":["32153\/8\/16.12.2024"],"award-info":[{"award-number":["32153\/8\/16.12.2024"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background\/Objectives: Agave amica (Medik.) Thiede &amp; Govaerts (Asparagaceae family) is an ornamental bulbous species, widely used for its fragrance but less studied as a medicinal species. This study is aimed at assessing the phenolic profile and selected biological properties of ethanolic extracts obtained from the aerial parts and bulbs of A. amica cultivated in Romania. Methods: The phenolic composition was characterized by spectrophotometric methods and LC\/MS analysis. The antioxidant activity was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity) and FRAP (Ferric reducing antioxidant power) tests, while the in vitro antimicrobial capacity was investigated by the agar-well diffusion, the broth microdilution, and the antibiofilm assays. Cytotoxicity was tested on a colorectal adenocarcinoma cell line (DLD-1) by a CCK-8 assay. Results: Both ethanolic extracts showed important polyphenol content and caffeic acid as their main compound. Significantly higher amounts of total polyphenols (44.25 \u00b1 1.08 mg\/g), tannins (12.55 \u00b1 0.34 mg\/g), flavonoids (9.20 \u00b1 0.19 mg\/g), caffeic acid derivatives (19.95 \u00b1 0.05 mg\/g), and also antioxidant activity (IC50 = 0.82 \u00b1 0.02 mg\/mL, and 79.75 \u00b1 1.80 \u00b5M TE\/g, respectively) were found for the aerial parts extract compared to the bulbs one (p &lt; 0.001). Notable anti-Candida albicans activity and moderate efficacy against Listeria monocytogenes and Staphylococcus aureus were displayed by both extracts against planktonic cells and biofilm. A dose-dependent cytotoxicity towards the colorectal adenocarcinoma cell line was recorded as well. Conclusions: This study brings novelty to the scientific literature by characterizing the phenolic profile and in vitro antimicrobial, antibiofilm, antioxidant, and antiproliferative activities of the ethanolic extracts obtained from A. amica, thus highlighting this herbal species\u2019s medicinal potential.<\/jats:p>","DOI":"10.3390\/antibiotics14070638","type":"journal-article","created":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T09:08:44Z","timestamp":1750669724000},"page":"638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Agave amica (Medik.) Thiede &amp; Govaerts (Asparagaceae)\u2014Insights into Its Valuable Phenolic Profile and In Vitro Antimicrobial, Antibiofilm, Antioxidative, and Antiproliferative Properties"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4181-0259","authenticated-orcid":false,"given":"Mihaela","family":"Niculae","sequence":"first","affiliation":[{"name":"Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5407-8001","authenticated-orcid":false,"given":"Daniela","family":"Hanganu","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Hattieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7660-9993","authenticated-orcid":false,"given":"Ilioara","family":"Oniga","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Hattieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergiu-Alexandru","family":"Burc\u0103","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Hattieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1333-5649","authenticated-orcid":false,"given":"Br\u00eendu\u0219a","family":"Tiperciuc","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, \u201cIuliu Ha\u021bieganu\u201d University of Medicine and Pharmacy, 41 V. Babe\u015f Street, 400012 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4098-6962","authenticated-orcid":false,"given":"Irina","family":"Ielciu","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Botany, Faculty of Pharmacy, \u201cIuliu Ha\u0163ieganu\u201d University of Medicine and Pharmacy, 400337 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7919-7381","authenticated-orcid":false,"given":"Emoke","family":"Pall","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 400372 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timea","family":"Bab","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Hattieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"},{"name":"PlantExtrakt Ltd., 407059 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4370-4668","authenticated-orcid":false,"given":"Ramona Flavia","family":"Burtescu","sequence":"additional","affiliation":[{"name":"PlantExtrakt Ltd., 407059 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihaela Andreea","family":"Sava","sequence":"additional","affiliation":[{"name":"Transylvania School of Botanic Art & Illustration, Cop\u0219a Mare, 557046 Sibiu, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8695-6123","authenticated-orcid":false,"given":"Daniela","family":"Benedec","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy, \u201cIuliu Hattieganu\u201d University of Medicine and Pharmacy, 400010 Cluj-Napoca, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"100081","DOI":"10.1016\/j.glmedi.2024.100081","article-title":"Antimicrobial Resistance: Impacts, Challenges, and Future Prospects","volume":"2","author":"Ahmed","year":"2024","journal-title":"J. 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