{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T11:40:37Z","timestamp":1785498037160,"version":"3.56.0"},"reference-count":83,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T00:00:00Z","timestamp":1750809600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation (OPS MHESI), Thailand Science Research","award":["RGNS 65-187"],"award-info":[{"award-number":["RGNS 65-187"]}]},{"name":"Office of the Permanent Secretary, Ministry of Higher Education, Science, Research and Innovation (OPS MHESI), Thailand Science Research","award":["WU-COE-65-05"],"award-info":[{"award-number":["WU-COE-65-05"]}]},{"name":"Centre of Excellence in Innovation of Essential Oil and Bioactive Compounds","award":["RGNS 65-187"],"award-info":[{"award-number":["RGNS 65-187"]}]},{"name":"Centre of Excellence in Innovation of Essential Oil and Bioactive Compounds","award":["WU-COE-65-05"],"award-info":[{"award-number":["WU-COE-65-05"]}]},{"name":"One Health Research Centre, Walailak University, Thailand","award":["RGNS 65-187"],"award-info":[{"award-number":["RGNS 65-187"]}]},{"name":"One Health Research Centre, Walailak University, Thailand","award":["WU-COE-65-05"],"award-info":[{"award-number":["WU-COE-65-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background: Pyoderma is a superficial bacterial infection that is considered the formation of pus-containing lesions on the skin occurring in animals. Staphylococci, including Staphylococcus aureus and Staphylococcus pseudintermedius, that cause pyoderma in pet animals is a global health concern. The objectives of this study were to investigate antibiotic-resistant staphylococci isolated from pyoderma in dogs and cats and to analyse whole genome sequences of multidrug-resistant (MDR) staphylococci. Methods: A total of 56 pyoderma swabbing samples from 42 dogs and 14 cats located in Southern Thailand was collected to isolate staphylococci. Antibiotic susceptibility and antibiotic-resistant genes of staphylococcal isolates were investigated. Furthermore, the representative MDR isolates were investigated using whole genome sequence analysis. Results: 61 isolates were identified as staphylococci, which can be classified into 12 different species, mostly including 13 S. intermedius (13.26%), 13 S. saprophyticus (13.26%), 8 S. sciuri (8.16%), and Staphylococcus cohnii (8.16%). Remarkably, the main pyoderma-causing species that were isolated in this study were S. aureus (5.10%) and S. pseudintermedius (3.06%). Most staphylococci were resistant to penicillin G (30%), and the blaZ gene was found to be the highest prevalence of the resistance genes. Both MDR-S. aureus WU1-1 and MDR-S. pseudintermedius WU48-1 carried capsule-related genes as main virulence factor genes. Interestingly, MDR-S. pseudintermedius WU48-1 was resistant to seven antibiotic classes, which simultaneously carried blaZ, mecA, aac, dfrK, aph3, and tetM. Genes related to antibiotic efflux were the highest proportion of the mechanism found in both representatives. Remarkably, SCCmec cassette genes were found in both isolates; however, the mecA gene was found only in MDR-S. pseudintermedius WU48-1. In addition, these were mostly carried by macrolide- and tetracycline-resistance genes. Mobile gene transfer and horizontal gene transfer events frequently contain genes involved in the antibiotic target alteration mechanism. Conclusions: This study found that MDR staphylococci, especially S. aureus and S. pseudintermedius, are important in animals and owners in terms of One Health concern. The information on whole genome sequences of these MDR staphylococci, particularly antimicrobial resistance genes, mobile genetic elements, and horizontal gene transfer events, can help to understand gene transmission and be applied for antibiotic resistance surveillance in veterinary medicine.<\/jats:p>","DOI":"10.3390\/antibiotics14070643","type":"journal-article","created":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T03:53:02Z","timestamp":1750823582000},"page":"643","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Whole Genome Sequence Analysis of Multidrug-Resistant Staphylococcus aureus and Staphylococcus pseudintermedius Isolated from Superficial Pyoderma in Dogs and Cats"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0432-0998","authenticated-orcid":false,"given":"Phirabhat","family":"Saengsawang","sequence":"first","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruedeechanok","family":"Tanonkaew","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rungruedee","family":"Kimseng","sequence":"additional","affiliation":[{"name":"Sanders-Brown Center on Aging, College of Medicine, University of Kentucky, Lexington, KY 40506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veeranoot","family":"Nissapatorn","sequence":"additional","affiliation":[{"name":"Futuristic Science Research Center, School of Science, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phitchayapak","family":"Wintachai","sequence":"additional","affiliation":[{"name":"School of Science, Walailak University, Thasala, Nakhon Si Thammarat 80161, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8744-7045","authenticated-orcid":false,"given":"Manuel J.","family":"Rodr\u00edguez-Ortega","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Universidad de C\u00f3rdoba, Campus de Excelencia Internacional CeiA3, 14071 C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9048-3955","authenticated-orcid":false,"given":"Watcharapong","family":"Mitsuwan","sequence":"additional","affiliation":[{"name":"Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"One Health Research Center, Walailak University, Nakhon Si Thammarat 80160, Thailand"},{"name":"Center of Excellence in Innovation of Essential Oil and Bioactive Compounds, Walailak University, Nakhon Si Thammarat 80160, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"796","DOI":"10.1177\/03009858231176558","article-title":"Common Superficial and Deep Cutaneous Bacterial Infections in Domestic Animals: A Review","volume":"60","author":"Faccin","year":"2023","journal-title":"Vet. 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