{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:28:58Z","timestamp":1760059738207,"version":"build-2065373602"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,5]],"date-time":"2025-07-05T00:00:00Z","timestamp":1751673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"European Union","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}]},{"name":"Merck Sharp &amp; Dohme (MSD)","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}]},{"DOI":"10.13039\/501100004587","name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas","doi-asserted-by":"publisher","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Axencia Galega de Innovaci\u00f3n (GAIN), Conseller\u00eda de Innovaci\u00f3n, Conseller\u00eda de Econom\u00eda, Emprego e Industria","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}]},{"name":"Axencia Galega de Innovaci\u00f3n (GAIN), Conseller\u00eda de Innovaci\u00f3n, Conseller\u00eda de Emprego e Industria","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}]},{"name":"Juan Rod\u00e9s program","award":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"],"award-info":[{"award-number":["PI22\/01212","PI20\/00686","PI21\/00704","CB21\/13\/00055","IN607A","IN607D2021\/12","IN607D2024\/008","JR21\/00026"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Antibiotics"],"abstract":"<jats:p>Background and Objectives: Infections caused by non-tuberculous mycobacteria (NTM), including Mycobacterium abscessus complex (MABc), are increasing globally and are notoriously difficult to treat due to the intrinsic resistance of these bacteria to many common antibiotics. The aims of this study were to demonstrate the in vitro activity of imipenem\/relebactam against MABc clinical isolates and to determine any in vitro synergism between imipenem\/relebactam and other antimicrobials. Methods: A nationwide collection of 175 MABc clinical respiratory isolates obtained from 24 hospitals in Spain (August 2022\u2013April 2023) was studied. Fifteen different antimicrobial agents were comprised, including imipenem\/relebactam. MICs were determined according to CLSI criteria, and the synergism studies were performed with the selected clinical isolates. Results: Of the 175 isolates obtained, 110 were identified as M. abscessus subsp. abscessus (62.9%), 51 as M. abscessus subsp. massiliense (29.1%), and 14 as M. abscessus subsp. bolleti (8%). The antibiotics yielding the highest susceptibility rates were tigecycline, eravacycline, and omadacycline (100%); followed by imipenem\/relebactam and clofazimine (97.6%); and finally amikacin (94.6%). Only four isolates were resistant to imipenem\/relebactam, three of which were further characterized by WGS, revealing MABc mutations in BlaMab as well as D,D- and L,D-transpeptidades and mspA porin, which may play an important role in reduced susceptibility to imipenem\/relebactam, even though none were previously described or associated with resistance to \u03b2-lactams. Conclusions: Our data demonstrate that relebactam improved the anti-MABc activity of imipenem, representing a \u03b2-lactam for the treatment of MABc infections. Furthermore, imipenem\/relebactam demonstrated in vitro synergism with other anti-MABc treatments, thus supporting its use as part of dual regimens.<\/jats:p>","DOI":"10.3390\/antibiotics14070682","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T10:03:27Z","timestamp":1751882607000},"page":"682","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["In Vitro Susceptibility to Imipenem\/Relebactam and Comparators in a Multicentre Collection of Mycobacterium abscessus Complex Isolates"],"prefix":"10.3390","volume":"14","author":[{"given":"Alejandro","family":"Seoane-Est\u00e9vez","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Aja-Macaya","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC, CB21\/13\/00055), ISCIII, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1156-4199","authenticated-orcid":false,"given":"Andrea","family":"Garcia-Pose","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7989-4275","authenticated-orcid":false,"given":"Paula","family":"L\u00f3pez-Roa","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Hospital 12 de Octubre (Imas12), 28041 Madrid, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5903-0453","authenticated-orcid":false,"given":"Alba","family":"Ruedas-L\u00f3pez","sequence":"additional","affiliation":[{"name":"Instituto de Investigaci\u00f3n Hospital 12 de Octubre (Imas12), 28041 Madrid, Spain"},{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario 12 de Octubre, 28041 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9302-7813","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Gonzalez-Gal\u00e1n","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Hospital Universitario Virgen del Roc\u00edo, 41013 Sevilla, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8971-3167","authenticated-orcid":false,"given":"Jaime","family":"Esteban","sequence":"additional","affiliation":[{"name":"Departmento de Microbiolog\u00eda Cl\u00ednica, Instituto de Investigaci\u00f3n Sanitaria-Fundaci\u00f3n Jim\u00e9nez D\u00edaz, 28040 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4248-503X","authenticated-orcid":false,"given":"Jorge","family":"Arca-Su\u00e1rez","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC, CB21\/13\/00055), ISCIII, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mart\u00edn","family":"Pamp\u00edn","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alejandro","family":"Beceiro","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC, CB21\/13\/00055), ISCIII, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina","family":"Ovia\u00f1o","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A Coru\u00f1a, Sergas, Universidade da Coru\u00f1a (UDC) As Xubias, 15006 A Coru\u00f1a, Spain"},{"name":"CIBER de Enfermedades Infecciosas (CIBERINFEC, CB21\/13\/00055), ISCIII, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8837-0062","authenticated-orcid":false,"given":"Germ\u00e1n","family":"Bou","sequence":"additional","affiliation":[{"name":"Servicio de Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complejo Hospitalario Universitario A 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